LOCUS       EF712256                 592 bp    DNA     linear   GSS 02-JUL-2007
DEFINITION  Mus musculus C57BL/6N IST10020A5HMF1 gene trap embryonic stem cell
            insertion site sequence tag (IST), genomic survey sequence.
ACCESSION   EF712256
VERSION     EF712256.1
KEYWORDS    GSS.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Hansen,G.M., Markesich,D., Zhu,Q., Richter,L.J., Abuin,A.,
            Sands,A., Finnell,R.H. and Zambrowicz,B.P.
  TITLE     Large-Scale Gene Trapping in C57BL/6N Mouse Embryonic Stem Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 592)
  AUTHORS   Finnell,R.H. and McLeod,M.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2007) TIGM, 2121 W. Holcombe Blvd, Houston, TX
            77030, USA
COMMENT     Contact: Richard H. Finnell at Texas Institute for Genomic Medicine
            (TIGM)  Phone: 713-677-7429  Fax: 713-677-7790  Email:
            ncbigss@tigm.org  Website: www.tigm.org.
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Mus musculus"
                     /mol_type="genomic DNA"
                     /strain="C57BL/6N"
                     /db_xref="taxon:10090"
                     /clone="IST10020A5HMF1"
                     /sex="male"
                     /cell_line="Lex3.13 ES transgenic cell line harboring
                     insertion mutation"
                     /cell_type="embryonic stem cell"
                     /transgenic
                     /note="strain origin confirmed on the basis of 110 genetic
                     markers distributed across 19 autosomes and the X
                     chromosome (Charles River Laboratories); sequence tags are
                     derived from genomic sequence by inverse PCR (IPCR) and
                     represent the gene-trap vector insertion site; sequence
                     reads represent either the upstream (F) or downstream (R)
                     vector-genomic junction, amplified from circularized
                     genomic DNA"
     source          1..30
                     /organism="Gene trapping vector VICTR75"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:447634"
     repeat_region   <1..30
                     /rpt_type=long_terminal_repeat
BASE COUNT          132 a          118 c          181 g          161 t
ORIGIN      
        1 gattgactac ccgtcagcgg gggtctttca aagggaagca aaggctggaa agttgcccag
       61 ggccaggcag aggtcaaagg atatcagagg gcaagcatcc tgttctctaa tagccagtga
      121 cttccaaccc tgccggtagg cctttgctga ttcccaattt atagatgggg aaacagaggc
      181 tccaagttcc aggaacggtg gcaccatgct cagtgctgag tctgtcttac ttgcagactg
      241 gggtttagtt cacttctgag agtctgggag aaactgagcc ctctgaccaa ggggaggaag
      301 gggaaggtaa ggatgagggt gatcacaggt ggcggtcctc cccacctatg cattggtcag
      361 attagggtca gattagggtc tcagctctac catatatgct gggaatctct gggtgttttt
      421 gctttgtttt gaaggatttg tttattttta atgcgcattg gtgttttgcc tgcatgcatg
      481 tctgtgtgag ggtgtcagat cctctgaaaa tggagttaca gatggttgtg agctgccatg
      541 tgagtgctgg gaactgaact cgggaccttt ggaagagctg ctggtgctct ta
//