LOCUS       EF555554                 888 bp    DNA     linear   BCT 30-MAY-2007
DEFINITION  Mycobacterium tuberculosis H37Rv polyphosphate kinase 2 gene,
            complete cds.
ACCESSION   EF555554
VERSION     EF555554.1
KEYWORDS    .
SOURCE      Mycobacterium tuberculosis H37Rv
  ORGANISM  Mycobacterium tuberculosis H37Rv
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis
            complex.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Kim,H.-Y. and Ravikumar,V.
  TITLE     Cloning and expression of polyphosphate kinase 2 from Mycobacterium
            tuberculosis H37Rv
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 888)
  AUTHORS   Kim,H.-Y. and Ravikumar,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2007) Department of Biochemistry, College of
            Oriental Medicine, Kyung Hee University, 1#, Hoegi-Dong,
            Dongdaemoon-Ku, Seoul 130-701, Korea
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Mycobacterium tuberculosis H37Rv"
                     /mol_type="genomic DNA"
                     /strain="H37Rv"
                     /type_material="type strain of Mycobacterium tuberculosis"
                     /db_xref="taxon:83332"
                     /PCR_primers="fwd_seq: gtggatataccatccgttgatgtgagc,
                     rev_seq: ccgggcgatcaacgtggccacatag"
     gene            1..888
                     /gene="ppk2"
     CDS             1..888
                     /gene="ppk2"
                     /function="converts GTP/ATP to poly P"
                     /note="virulance factor"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="ABQ58103.1"
                     /translation="MDIPSVDVSTATNDGASSRAKGHRSAAPGRRKISDAVYQAELFR
                     LQTEFVKLQEWARHSGARLVVIFEGRDGAGKGGAIKRITEYLNPRVARIAALPAPTDR
                     ERGQWYYQRYIAHLPAKGEIVLFDRSWYNRAGVEKVMGFCTPQEYVLFLRQTPIFEQM
                     LIDDGILLRKYWFSVSDAEQLRRFKARRNDPVRQWKLSPMDLESVYRWEDYSRAKDEM
                     MVHTDTPVSPWYVVESDIKKHARLNMMAHLLSTIDYADVEKPKVKLPPRPLVSGNYRR
                     PPRELSTYVDDYVATLIAR"
BASE COUNT          188 a          243 c          278 g          179 t
ORIGIN      
        1 gtggatatac catccgttga tgtgagcacc gcgaccaacg acggcgcgtc gtcgagagcg
       61 aagggacaca gatcagctgc gccgggccgg cgcaagatct ccgatgccgt ctaccaagcc
      121 gaattgtttc ggctgcaaac agaattcgtg aagctgcaag agtgggcccg gcattcgggt
      181 gcgcgtctgg tggtcatctt cgaaggccgt gacggagctg gtaagggtgg cgccatcaaa
      241 cggatcaccg agtacctcaa cccgcgagtg gctcgtatcg cggcattgcc cgcgccgacg
      301 gatcgcgagc ggggtcagtg gtactaccag cgttatattg cccatcttcc cgccaaaggt
      361 gagatagtgc tcttcgatcg gtcctggtac aaccgcgccg gtgtggagaa ggtcatgggt
      421 ttctgtacgc cgcaggagta tgtgttgttt ttgcggcaga ccccgatttt cgagcagatg
      481 ctgatcgatg acgggattct gcttcgcaaa tactggtttt cagtctcaga cgccgaacag
      541 ttgcgccgat tcaaggctcg acggaatgac ccggtccggc aatggaagct cagcccaatg
      601 gacctggaat cggtgtatcg gtgggaggac tattcgcgcg caaaggacga gatgatggtg
      661 cataccgaca cccccgtcag cccctggtac gtggtggagt ctgatatcaa aaagcacgcg
      721 cggctgaaca tgatggccca cttgctgtcc acgattgact acgcagatgt ggaaaagcca
      781 aaggtcaagc tgccgccacg gccgcttgtg agtggcaact acaggcgtcc gccccgcgag
      841 ttgtcgacat acgtcgacga ctatgtggcc acgttgatcg cccggtga
//