LOCUS DUOG01000120 14135 bp DNA linear ENV 25-APR-2020 DEFINITION MAG TPA_asm: Acholeplasmataceae bacterium isolate AS08sgBPME_156 305338_AS08, whole genome shotgun sequence. ACCESSION DUOG01000120 DUOG01000000 VERSION DUOG01000120.1 DBLINK BioProject: PRJNA602310 BioSample: SAMN13893783 Sequence Read Archive: SRR2917896, SRR2917897, SRR2917898 KEYWORDS WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA; TPA:assembly. SOURCE Acholeplasmataceae bacterium (anaerobic digester metagenome) ORGANISM Acholeplasmataceae bacterium Bacteria; Tenericutes; Mollicutes; Acholeplasmatales; Acholeplasmataceae. REFERENCE 1 (bases 1 to 14135) AUTHORS Campanaro,S., Treu,L., Rodriguez-R,L.M., Kovalovszki,A., Ziels,R.M., Maus,I., Zhu,X., Kougias,P.G., Basile,A., Luo,G., Schluter,A., Konstantinidis,K.T. and Angelidaki,I. TITLE New insights from the biogas microbiome by comprehensive genome-resolved metagenomics of nearly 1600 species originating from multiple anaerobic digesters JOURNAL Biotechnol Biofuels 13, 25 (2020) PUBMED 32123542 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 14135) AUTHORS Campanaro,S. TITLE Direct Submission JOURNAL Submitted (28-JAN-2020) Department of Environmental Engineering, Technical University of Denmark, Bygningstorvet, Bygning 115, Kgs. Lyngby 2800, Denmark COMMENT The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Assembly-Data-START## Assembly Date :: JUN-2018 Assembly Method :: Megahit v. 1.1.1 Genome Representation :: Full Expected Final Version :: No Genome Coverage :: 210.5200622525x Sequencing Technology :: Illumina HiSeq 2500 ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Date :: 04/10/2020 15:39:48 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Method :: Best-placed reference protein set; GeneMarkS-2+ Annotation Software revision :: 4.11 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total) :: 1,736 CDSs (total) :: 1,685 Genes (coding) :: 1,670 CDSs (with protein) :: 1,670 Genes (RNA) :: 51 rRNAs :: 2, 2 (5S, 16S) complete rRNAs :: 2 (5S) partial rRNAs :: 2 (16S) tRNAs :: 40 ncRNAs :: 7 Pseudo Genes (total) :: 15 CDSs (without protein) :: 15 Pseudo Genes (ambiguous residues) :: 0 of 15 Pseudo Genes (frameshifted) :: 3 of 15 Pseudo Genes (incomplete) :: 11 of 15 Pseudo Genes (internal stop) :: 1 of 15 CRISPR Arrays :: 2 ##Genome-Annotation-Data-END## FEATURES Location/Qualifiers source 1..14135 /organism="Acholeplasmataceae bacterium" /mol_type="genomic DNA" /submitter_seqid="305338_AS08" /isolate="AS08sgBPME_156" /isolation_source="anaerobic digestion of organic wastes under variable temperature conditions and feedstocks" /db_xref="taxon:1898209" /environmental_sample /metagenome_source="anaerobic digester metagenome" /note="metagenomic" gene 95..1060 /locus_tag="GXZ35_07785" CDS 95..1060 /locus_tag="GXZ35_07785" /inference="COORDINATES: protein motif:HMM:NF013528.1,HMM:NF014342.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="bifunctional oligoribonuclease/PAP phosphatase NrnA" /protein_id="HHU81206.1" /translation="MLQKIFDKIKEYNRIIIHRHIRPDGDCIGSQFGLKYIIQATYPE KEVYAVGHDVPEYLSYIGQGDQVSNDLYEGALVIVVDTATTGRIFDERYKNGDYLIKI DHHDDSEDYGDIIWVDVKAPATSAMIAKFYYTFKDQLVLSKEAARALFSGMVTDTGRF KYGNLTGDTFRYAGEMIDAEIDIDGLYANLYVKDAAVYKLQGYTYSNFKMTDNGVSYI YMTKKIMKKYGIAPDEAANLVNTLDSIKGSLIWVAFVDQPDNSIRVRLRSRFVPINDI GRKYNGGGHLRAAGATLYHKSEIKSILKDLDQRIKAFKDENKGVQ" gene 1061..1789 /gene="surE" /locus_tag="GXZ35_07790" CDS 1061..1789 /gene="surE" /locus_tag="GXZ35_07790" /EC_number="3.1.3.5" /EC_number="3.1.3.6" /inference="COORDINATES: protein motif:HMM:TIGR00087.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="5'/3'-nucleotidase SurE" /protein_id="HHU81207.1" /translation="MKVLVVNDDGIESIGLKLLVEKLIPYAEEIMVVAPTYEQSAQSH SLTVRRGIKLTNHEPLLDRIKTYSIDGSPADCVKFAYNHLKYDFDIVFSGVNNGLNLG DDIIYSGTVAGASEAAFMHRKGIAFSSPKNDFSGFVKYFDQIMDYLLKSKVYQNSLVL NINIPTVAKGINITHQGHHPYNTEFVLKDDNLFYSIGKYDEIENDENSDIFSFNAGYI SITPVTVNRTDLTSFKDNHELIKF" gene 1845..2027 /locus_tag="GXZ35_07795" CDS 1845..2027 /locus_tag="GXZ35_07795" /inference="COORDINATES: protein motif:HMM:NF019085.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="HHU81208.1" /translation="MLKFIKWMLKSILLGVVIIFVFNIIGVYLNLNIPVNVWTIIIVG ILKVPGLIMLLILSII" gene 2048..2434 /locus_tag="GXZ35_07800" CDS 2048..2434 /locus_tag="GXZ35_07800" /inference="COORDINATES: protein motif:HMM:NF020711.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="DUF1934 domain-containing protein" /protein_id="HHU81209.1" /translation="MKKEVMVNFLIEATDGSSVTFMTPGTLIDYEDHKELTFIEENDA KLETTVSIYPAHLVIERTGDLKMKMEFKIGQTTYVKMKTNFKFELTMACVTHSLLIEP NLIDVIYQTDTDRENNITHHLRIKFE" gene 2483..3037 /gene="rpoE" /locus_tag="GXZ35_07805" CDS 2483..3037 /gene="rpoE" /locus_tag="GXZ35_07805" /EC_number="2.7.7.6" /inference="COORDINATES: protein motif:HMM:TIGR04567.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="DNA-directed RNA polymerase subunit delta" /protein_id="HHU81210.1" /translation="MATKESDFSLIEIAYKLLSKKRKPQLLDDIIKDVMSTKGYNVKE AKEIAPQFILDFQTCGDFIYCGDGRWDLKERQPTSVLDKDGSDYDYEDDEDVKKNELK EDDFIRIHKDLDVIDDEDEEDQLGDEETEEDDLAAEFETLEDDEDDLDFDDDEDEDLD LDDEEDPLEHLIFDEDEDEEEEEE" gene 3201..4802 /locus_tag="GXZ35_07810" CDS 3201..4802 /locus_tag="GXZ35_07810" /EC_number="6.3.4.2" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_017436903.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="CTP synthase" /protein_id="HHU81211.1" /translation="MKQNTKFIFVTGGVVSSLGKGIVASSLGRLLKNRGLKVFMQKFD PYINYDPGLMSPYQHGEVYVTEDGAETDLDLGHYERFIDEFLSKNSNITTGKIYKKVI DKERNGEYNGKTVQVIPHITDEIKEYLIKAAESSGADVVITEIGGTVGDIEGLPFLEA IRQARRDFGYHNTLYIHITLVPFLKAANEIKTKPTQHSVKELRSLGIQPDVIVLRSEV SVPKEQKEKIALMCDVDKEAVIESVDQKILYELPNKLKEQRLDDIVCAHLKIDTPAAD MTEWNKMINQIKKAKDSVRIAIVGKYVALQDAYISVVESLRHAGYIYGKKIDLKWVDA DTITKDNCEEMFGDVKGILVPGGFGERGLEGKIQAATYARARKVPYFGICLGMHIALI DIARNLCKLEDANSEEADNESKNKIIIELPKKERKDNFEKKLRLGSCEAVLEEGSKVY EAYQEKVILERHRHRFHFNNKYRSLFEQNGVVFSGNDREASIVETMELKNHPWFVGCQ FHPEFKSRPNRPHPLFKAFIESCIK" gene 4892..6127 /locus_tag="GXZ35_07815" CDS 4892..6127 /locus_tag="GXZ35_07815" /inference="COORDINATES: protein motif:HMM:NF014069.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="HD domain-containing protein" /protein_id="HHU81212.1" /translation="MDEIKVFRDPVYGYISVESEIIWDLVNTREFQRLRKIHQLGGVA YVFHTANHTRFDHSLGTYHIASRLVKEIEGLKELLEERGVTLFLVTSLLHDIGHGSFS HAFEEAFDINHENYTIKVILGDTEVNKVLRKHDPNLPQDVASIIAKEGRFPLLESLIS SQLDSDRLDYLVRDTYFTGATYGHIDTDRIIRIMRVIDHSVVYKASGIHAIEDYLMSR FHMYWQVYFHPVGRSFEVMLNKLYLRIKELYNEGFVFSGLVLKLIDFMNNPDAISKYL ILNDYLIMSMVDELAEKNIDDILYHLANRISGRRLFKYVINNDVEHIYAQFEQELKNK GYNPNYYLHVDNVKQIVYNNKYSIEKADSIRILTENGEIVPIHEYSEIVKSLVYAAGK SEIRLFYDKELVDPSKEGN" gene 6127..6984 /gene="rsmA" /locus_tag="GXZ35_07820" CDS 6127..6984 /gene="rsmA" /locus_tag="GXZ35_07820" /EC_number="2.1.1.182" /inference="COORDINATES: protein motif:HMM:NF001349.0" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="ribosomal RNA small subunit methyltransferase A" /protein_id="HHU81213.1" /translation="MRPSDINKLLERYNLKAKKHLGQNFLSDQNYINKIVDSASIDKN TAVIEIGPGLGSLTQKLVEKAGFVLCYEIDSDMANIIKDTIEAPNLVLINQDFLRANV FDDIKEYLGQYEEIILVANLPYYITTAILIKILEDRLPIKKMIVMMQKEVADRLSGNP KTKEYNSLSVLVQYYTNVKRLFEVAPDAFIPRPEVVSTVLELEKKISIETKPLNEEFF LKFNRLIFSSRRKTISNNIAKGLNIDKKIIAETLVENGFRADIRAEALSVADIIKLSD LFYQIKKED" gene 7038..7805 /locus_tag="GXZ35_07825" CDS 7038..7805 /locus_tag="GXZ35_07825" /inference="COORDINATES: protein motif:HMM:NF017401.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="HHU81214.1" /translation="MFEIGQKVFIKGDRNIYIIDNIVNDDYVLTGYSYRVIKRTVKDN IIPIEENDIIIEDQKNERYKNKVTSLPRGIKKNKILFGRVLHIDGDQEYLKRCLNLYK ELGIPAEGIYIKENQMSKSIGELVDTLTPDIIVITGHDGYNKKGLNDLDNYENTKNFI DAIRVVRRKFNNNEVVIIAGACGSHFEALIGSGANFASSPKRVNTHTYDPAIVAIKVA STSYNRLIDFNDIIKYIENGRDAIGGIETNGKMRLIL" gene 7818..8669 /gene="ispE" /locus_tag="GXZ35_07830" CDS 7818..8669 /gene="ispE" /locus_tag="GXZ35_07830" /EC_number="2.7.1.148" /inference="COORDINATES: protein motif:HMM:TIGR00154.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase" /protein_id="HHU81215.1" /translation="MIKAYAKVNLRLKVVGKRPNGYHDLEMINISVDLSDSLSIKKNK SGDIIIRCNNPLLPTDDNNIVHKVVKYIKETYQISDGFEIMIDKKIPIGAGLGGGSSD AAAMINWFNNEYRLNLSEDALIGLALKFGADVPYCLYGQPAIVTGIGEEINFIKLNLP KYLLLITPPINVSTKEIFNDYDRLISKENSDLNYRTNIYKKQKNNELMENDLMEVTFN KHPILEKMKRFLESLSKRKVMMSGSGPSLFCLFDSLNEANQIKDQYQERYPNHYINVT QIINVEK" gene 8693..9208 /locus_tag="GXZ35_07835" CDS 8693..9208 /locus_tag="GXZ35_07835" /inference="COORDINATES: protein motif:HMM:NF025675.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="YcxB family protein" /protein_id="HHU81216.1" /translation="MEVKFTQLVGKEDIYNYNMFFFTKKSKMMYFSYIVGFLSLGLGI YYLIKGEIPVGIICILLFAYSVFLHRLFTKWQIRKMIDRKTDIDNMTVFVEIDEEGFT YSNEEENTGNKLLWDNVLELYDYKNYWYIYYAPNRAVFVRKSDCPNVNSVETMLKEKL SYRYKTVKARD" gene 9334..9588 /gene="spoVG" /locus_tag="GXZ35_07840" CDS 9334..9588 /gene="spoVG" /locus_tag="GXZ35_07840" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_003756429.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="septation regulator SpoVG" /protein_id="HHU81217.1" /translation="MEITDVRIRKIEGQTRLKAIASITIDNAFAVHELRIIEGGNGYF VAMPSRKTQNNVFRDIAHPINSETRQMIEKVVLDKFNEAE" gene 9725..10531 /locus_tag="GXZ35_07845" CDS 9725..10531 /locus_tag="GXZ35_07845" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_004616481.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="transketolase" /protein_id="HHU81218.1" /translation="MQSLQKVAKEIRKLTARTIASIGAGHIGGSLSIVDVLTVLYFKH MNVDPKNPKMAGRDRLIVSKGHAGPGVYATLAYKGFFPIEELDTLNKIGTNLPSHCDM NRTKGIDMTTGSLGQGISCAVGIAIASRLARDNAHIYAIVGDGESQEGQVWEAAMYAS QQKLDNLIVFLDYNKYQLDGSTCDINDLGNVVDKWKSFGFNTVYIDGHNLEEIDKAIV NAKAHTGSPSMIILNTVKGKGISFAEGAGADSHSMPISKDQLQQAYKELE" gene 10543..11460 /locus_tag="GXZ35_07850" CDS 10543..11460 /locus_tag="GXZ35_07850" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007439791.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="transketolase family protein" /protein_id="HHU81219.1" /translation="MEMRQMLAKTLAEIMEKNDKIVVIDADLSKPNGTSVLYSKFPDR CFNVGIAEQNMMGIAAGIASYGYIPVVVTFTPFATRRPCDQLTVSVAYAKQNVKIIGT DPGLTAELNGGTHMSVEDVGIARSIPEILIYDAVDPLQLGQALPQIIDYEGAAYVRMP RKYAESVLDENYKFDLFKADILREGEDITVFATSIMVKESLVAAEILAQEGIDVEVVN VHTFKPLDEETIINSLKKTKKAIVCENHNIIGGLFSAISELVVQKYPVPIRPLGIKDR FGQVGKLPELMKEYEMTADDVVKLVKSMF" gene 11481..12413 /locus_tag="GXZ35_07855" CDS 11481..12413 /locus_tag="GXZ35_07855" /inference="COORDINATES: protein motif:HMM:NF016983.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="YcjF family protein" /protein_id="HHU81220.1" /translation="MNKKGKFFWILIGIGLFIIFVLMIVSSVIEVGERLRSFGQVVEI TFYVLCAFVFYFLIVNPIRIILFSPSFSIQTTFDKDNGKSKKVYKKVALNMLKNPDLS EEDKELLKEGLKDRTELKHQLQHVFDHSIKNSLNKIIIKNAKTVMVSTAISQNGRLDL FTVMAVNIKMIKELVVKCGFRPSFKNLGKLTANVFVTALIAEGLEDLDFNEIFPNSTK NFLSDIPFIKPIASSIMQGISNALLTIRVGIVTRKYLFSDGKEITKQEVRKYALKESL KILPVVIKQSLSVFPDRIKKMFTPKKHANEAESN" gene 12461..13426 /locus_tag="GXZ35_07860" CDS 12461..13426 /locus_tag="GXZ35_07860" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_006784949.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="ribose-phosphate pyrophosphokinase" /protein_id="HHU81221.1" /translation="MAIINGVKVKIFTLNSNPALAKEIADYVGIELSKCEISRFADGE VQIDIPETVRGHSVFVVQSTNKPVNENYMELLIMIDALKRASARSINVIMPYYGYSRQ DRKAAARQPISAKLVADLLQAAGANRVICMDLHADQIQGFFNIPIDNFVALPILADYF VQQEIKDIVVVSPDHGGTTRARRLANVFDAPIAIVDKRRPKPNVAEVMNIIGDVKGKN AIIIDDMIDTAGTITLAATALKKFGAKEVYACCTHPILSDPAIERLANSDIKEVITTN TIALDETKKISKIKQLSVAKLFGQGILNIIDDRPVSDLFRYNHKH" gene 13499..13735 /locus_tag="GXZ35_07865" CDS 13499..13735 /locus_tag="GXZ35_07865" /inference="COORDINATES: ab initio prediction:GeneMarkS-2+" /note="Derived by automated computational analysis using gene prediction method: GeneMarkS-2+." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="HHU81222.1" /translation="MVCKRCKTEINGALQDCPFCEIPIVEYKIKKVITRRNVVVDTSI IKTYKLASKMLKKISSNETAYKINCYKDAYFLNI" gene 14053..>14135 /locus_tag="GXZ35_07870" rRNA 14053..>14135 /locus_tag="GXZ35_07870" /product="16S ribosomal RNA" BASE COUNT 5160 a 1936 c 2681 g 4358 t ORIGIN 1 ggccatttaa acgccagtgg cgcaactatt gatagttttg aagttgccga taaattattg 61 aaagatttag ataaattaat gataggtgaa gaaaatgtta caaaaaatat ttgataaaat 121 taaagaatat aatcggatta ttatccatcg tcatattcgc cctgatgggg attgtattgg 181 aagtcaattt ggattaaagt atattattca agcgacttat ccagaaaaag aagtatatgc 241 tgtagggcac gatgttcctg aataccttag ctatattgga caaggtgatc aagtaagtaa 301 tgatctctat gaaggagcat tagtaattgt tgtcgataca gcaaccactg gtagaatctt 361 tgatgaacgt tataaaaatg gtgattattt aattaaaatt gatcatcatg atgattcaga 421 agattatggt gatattattt gggttgatgt taaagcccca gcaactagtg ctatgattgc 481 taaattttat tatactttca aagaccaatt agttttaagc aaagaagctg ctcgtgcttt 541 attttctgga atggtaaccg atactggccg atttaagtac ggtaatctta ctggtgatac 601 cttccgttat gctggcgaaa tgattgatgc agaaattgat attgatggtt tatatgctaa 661 tctctatgtt aaagatgctg ctgtttataa acttcaagga tatacttatt ctaattttaa 721 gatgactgat aatggtgtat catatatata catgactaaa aagattatga aaaagtatgg 781 aattgctcca gatgaagctg ctaatcttgt taatactctt gatagtatta aaggatcatt 841 aatatgggta gcatttgttg atcaaccaga taattcaata cgagttcgtc tccgttcgcg 901 ttttgtgcca ataaacgata ttggtagaaa gtataatggt ggtggacatc taagagctgc 961 tggtgctact ttatatcata agagtgaaat caagagtatc ttaaaagatc ttgatcaacg 1021 aattaaagcg ttcaaagatg aaaacaaagg agtccaatag atgaaggtat tggtcgtaaa 1081 tgatgatgga attgagtcaa ttggactaaa attgctagtt gaaaaattaa ttccttatgc 1141 tgaagaaata atggtggtgg caccaactta tgaacagtct gctcaaagtc attcattaac 1201 tgttcgaaga ggtataaaac taaccaatca tgaaccatta ctagatagaa tcaaaaccta 1261 ctcaatcgat ggttccccag cagattgtgt caagtttgct tacaaccatt taaaatatga 1321 ttttgatatt gtattttccg gcgtcaataa tggacttaat ctaggtgatg atatcatata 1381 ttctggaacc gtagccggag caagtgaggc agcctttatg caccgtaaag gaatagcttt 1441 ttcaagtcct aaaaatgatt ttagtggttt tgttaaatac tttgatcaaa taatggacta 1501 cttattaaaa agtaaagtat atcaaaatag tttggtgtta aatatcaaca tcccaacggt 1561 tgcgaaggga attaatatta ctcatcaagg acatcatcct tacaacacgg aattcgtttt 1621 aaaagatgat aatctattct attcaattgg gaaatacgat gaaatagaaa atgatgaaaa 1681 cagtgatatc ttcagtttta acgctggata tatatcaatt acaccggtaa cggttaatcg 1741 tactgatttg actagcttta aggataacca tgaactaatc aagttttaaa gcatgaatgg 1801 tctatttgga cattcttact catatttatt attaggtgat ggttatgtta aagtttataa 1861 aatggatgct aaaatctatt ttattaggag ttgttattat ttttgtcttt aatataattg 1921 gagtgtatct taacctgaat attccagtta atgtttggac aataatcatt gttggaatct 1981 taaaggttcc cggcttaata atgttgctaa tcttatcaat tatttaaata ggagaatatg 2041 ttatttcatg aaaaaagaag taatggtaaa ctttttaata gaagctactg atggtagttc 2101 ggtaacattt atgactccag gaacactgat cgattatgaa gatcataaag aactaacttt 2161 cattgaagaa aatgatgcta aactagaaac gactgtttcg atctatccgg ctcatttagt 2221 tattgaacgc acgggcgatt tgaaaatgaa aatggagttt aaaattggac aaactaccta 2281 tgttaagatg aaaaccaatt ttaagtttga attgacgatg gcttgtgtga cgcacagttt 2341 attgattgaa ccgaacttga tcgatgttat ttatcaaaca gatactgatc gagaaaataa 2401 tataactcat catctacgaa taaaatttga gtaaaagtaa taatgttaaa aatttaaggt 2461 aatatatata tggaggaata atatggcgac taaagaaagt gatttttcac taatagagat 2521 tgcttataaa ttgttaagta agaaaagaaa accacaatta ttagacgaca ttataaaaga 2581 tgtaatgtca accaagggct ataatgttaa agaagccaaa gaaatcgctc ctcaattcat 2641 attagatttc caaacatgtg gagactttat ttattgtgga gacggaagat gggacttgaa 2701 agaacgtcaa ccgacatcag tacttgataa agacggtagc gattatgatt atgaagacga 2761 tgaagatgtt aagaaaaacg aacttaaaga agatgacttc atcagaattc ataaagatct 2821 tgacgttatt gatgatgagg atgaagaaga tcaacttgga gatgaagaaa ctgaagaaga 2881 tgatttagct gctgaatttg aaactttaga agatgatgaa gatgatctag actttgacga 2941 tgatgaagat gaagacctag atttggatga tgaagaagac cctctggaac acttaatatt 3001 tgatgaagac gaagatgagg aagaagagga agaataaaac tttataaaaa tcgtggttaa 3061 aaatattaac atctgatata atttaaaaga gcctaaaata actctcgtag cagagttatt 3121 ttttatttat ggtggtatcc atcaattgat ggtatgggct ctcctttata aattaataat 3181 aaatagcgag gagtgcaaac atgaaacaaa atactaaatt tatttttgtc actggtggag 3241 ttgtttccag tttaggtaaa ggaattgttg cttcaagttt agggcgatta ttaaagaatc 3301 gcgggttaaa agtctttatg caaaagtttg acccatatat taattatgac ccgggtctaa 3361 tgtcacccta tcaacacggg gaagtttatg ttactgaaga tggggcggaa actgatttag 3421 acttagggca ttatgaaaga tttattgatg agtttttatc taaaaattct aatattacaa 3481 ctggaaagat atataaaaaa gttatcgata aagaacggaa tggcgaatac aatggtaaaa 3541 cggtacaagt tattcctcat attaccgacg aaattaaaga atatttaatt aaagcagcgg 3601 agtcttctgg agctgatgta gttattactg aaatcggagg aacagtgggt gatattgagg 3661 gactaccatt cctggaggca attagacaag ctcgacgtga ttttggttat cataatactt 3721 tatatataca cattacattg gtaccattct taaaagcagc aaatgagatt aaaaccaaac 3781 ctactcaaca tagtgttaaa gaactacgta gtttaggaat tcaaccagat gtaattgttc 3841 ttagaagcga agtatccgtg ccaaaagaac aaaaagaaaa aatagcatta atgtgtgacg 3901 ttgataaaga agcagtaatc gaatcagttg atcaaaaaat cttatatgaa ctacctaata 3961 aacttaaaga acaacgtcta gatgatattg tttgtgccca cctaaaaata gatacaccag 4021 cagctgatat gactgagtgg aataaaatga ttaatcaaat caaaaaggca aaagattcag 4081 ttcgaatagc gattgttgga aaatatgtgg cgttacaaga tgcctatata tcagttgtag 4141 agagtttgcg tcatgcagga tatatatatg gaaagaaaat tgacctaaaa tgggttgatg 4201 ctgatactat caccaaagat aattgcgaag aaatgtttgg tgatgtgaaa gggatactag 4261 ttcccggtgg ttttggtgaa aggggacttg aaggaaaaat tcaagccgca acctacgcca 4321 gagctcgaaa agtgccttac tttggaattt gtttaggaat gcatatcgct ttaatagata 4381 tagcaagaaa cctctgtaaa ttagaggacg ctaactctga agaagctgat aacgaaagca 4441 aaaataaaat aattattgaa ttaccgaaaa aagaaagaaa agataatttt gagaaaaagt 4501 taagattagg ttcatgtgag gcagttcttg aagaaggatc aaaagtttat gaagcatatc 4561 aggaaaaagt aattcttgaa agacatcgcc atcgcttcca ttttaacaat aaatatcgta 4621 gcctatttga acaaaatggt gtagtctttt cgggtaatga tagagaggcc agtatagtgg 4681 aaacgatgga actaaaaaac catccatggt ttgttggatg tcaattccac ccggaattta 4741 aatctcgtcc aaatcgccca catccgttat ttaaggcctt tattgaaagt tgtattaaat 4801 aaaaatggat tttattgtat aaatttattg aagattggta atgtgtgtta taatgggtat 4861 gaaaaatatt gaaaaaagat tggagtttaa tatggatgaa ataaaagttt ttcgtgatcc 4921 ggtctatgga tatatatcag tggaaagtga aattatctgg gatttagtaa acacccgtga 4981 atttcaaaga ttacggaaga ttcatcaatt aggtggagta gcttatgtat ttcatacggc 5041 caaccatacc cgttttgacc atagtttagg gacatatcat attgcctctc gattagttaa 5101 agaaatcgaa ggattaaaag aattattaga agaaagagga gttacccttt ttcttgtgac 5161 aagtttactt catgatattg gtcacggttc attctctcat gcctttgagg aggcctttga 5221 tattaatcat gaaaactaca ccattaaagt tatcttgggt gatactgaag taaataaagt 5281 tttaagaaaa catgatccta acttaccaca agatgttgct tctattatcg caaaagaagg 5341 tagattccct ttacttgaat ccttaatatc tagtcaatta gatagtgatc gtttggatta 5401 tttagtaaga gatacttact ttaccggagc gacctatgga catattgata ccgatcgaat 5461 aattcgcatt atgagagtaa ttgatcactc ggttgtctat aaagccagtg gtattcatgc 5521 cattgaagat tatttgatga gtagatttca tatgtactgg caagtatatt tccatccagt 5581 cggacgaagc tttgaagtaa tgttaaataa attatattta agaattaaag aattatataa 5641 tgaaggcttt gtttttagtg gtctagtatt aaagttaatt gactttatga ataatccaga 5701 tgctataagc aagtatctta ttttaaatga ctatttgatt atgtcaatgg ttgacgaact 5761 tgctgagaaa aacattgatg atatcttata tcatctagca aatagaatct ccggaagaag 5821 acttttcaaa tatgtaatta ataatgatgt tgaacatata tatgcacaat ttgaacaaga 5881 actaaaaaac aaagggtata atccaaatta ttaccttcat gtcgataatg taaagcaaat 5941 tgtttataat aataaatatt cgattgaaaa ggccgacagc attcgaattt taaccgagaa 6001 cggtgaaatt gtaccaattc atgaatattc tgaaattgtc aaaagtctcg tatatgcggc 6061 tgggaaatcg gaaattagac tgttttatga taaagaacta gttgacccta gcaaggaagg 6121 taattaatgc gtccctctga tattaataaa ttgttagaaa gatataactt aaaggccaaa 6181 aaacatcttg gccaaaactt tctcagtgat caaaattata ttaataaaat cgttgattcc 6241 gcttcaattg ataaaaacac agcagtaatt gaaattggac cgggacttgg ttcccttact 6301 caaaaactag tagaaaaagc tggttttgtg ctctgttatg aaattgatag cgatatggca 6361 aacatcataa aagataccat tgaagcacca aatctagtac ttattaatca agatttctta 6421 agggctaatg tattcgatga tattaaggaa tatctaggtc aatatgaaga aattatttta 6481 gtagctaatc ttccttatta tatcaccaca gccatcctaa taaagatatt agaagatcgt 6541 ttaccgatca aaaaaatgat tgtgatgatg caaaaagaag tagctgatcg attaagtggg 6601 aatccaaaga ccaaagaata taattcgttg agcgtattgg ttcaatatta taccaatgta 6661 aaaaggttgt ttgaggttgc gccagatgct tttataccac gaccagaggt tgtaagcaca 6721 gtattggaat tagaaaaaaa gatatcgatt gagacaaaac cattaaatga agaattcttc 6781 ttaaagttta atcgactaat ctttagcagt cgccgaaaaa cgataagtaa taatatcgcc 6841 aaaggattga atatcgataa aaagataatt gctgagacac ttgtagaaaa tggttttcgt 6901 gctgacataa gagcggaagc cttatctgtt gctgatatta ttaaattaag tgatcttttt 6961 tatcaaataa aaaaagaaga ctaattttta tttcttaatc aaaaaatact tcatataatt 7021 ataaagaggt gttttttgtg tttgaaatag gacaaaaagt ttttattaaa ggcgaccgaa 7081 acatatatat aattgataat attgtcaatg atgattatgt cttgactggt tattcttatc 7141 gtgtgattaa aagaaccgta aaagataata tcatccctat tgaagaaaat gatattatta 7201 tcgaagatca aaaaaacgaa cgttacaaaa ataaagttac atctttaccg cgaggcatta 7261 aaaaaaacaa gatacttttt gggcgcgtct tacatattga tggcgatcaa gagtacttga 7321 aaagatgctt aaatctatat aaagagttag gcataccagc ggaaggtata tatataaaag 7381 aaaatcaaat gtctaaatcg attggcgaat tagttgatac attaacaccg gacatcattg 7441 taataacagg tcatgacggc tataataaaa aaggactaaa tgatttagat aactatgaaa 7501 atactaaaaa ctttattgat gcgattagag tagtcagaag aaagtttaac aataatgaag 7561 tagtaataat tgcgggagct tgtggttctc actttgaggc tttgattggc agcggggcta 7621 actttgcctc tagtccgaaa agagtaaata ctcatacata tgaccctgcc atagtcgcaa 7681 ttaaagtagc ttctacttca tataatcgat taattgattt taatgacatt ataaaatata 7741 ttgaaaatgg tcgcgatgcg atcggtggga ttgaaacaaa cggtaaaatg cgtcttattt 7801 tataagttgg tgaagatatg ataaaagcat atgcaaaggt gaatttgagg cttaaagtag 7861 ttggaaaaag gcctaatggc tatcatgact tagaaatgat aaatataagt gttgatttat 7921 cagatagctt aagcattaaa aagaataaaa gtggagatat tattattaga tgtaataacc 7981 cgcttcttcc aactgatgat aataatattg tccataaagt agtaaaatat atcaaagaaa 8041 cctatcaaat atctgatgga tttgaaatta tgattgataa aaaaatcccc attggtgctg 8101 gcctgggtgg gggaagtagt gacgcagcag ccatgattaa ttggtttaat aatgaatatc 8161 gactgaatct ttccgaagat gctttgattg gactagccct aaagtttggt gctgatgttc 8221 cctactgtct atatggacaa cccgcaatag tcaccgggat tggtgaggaa attaacttca 8281 ttaaacttaa tttacccaaa tatttattgc ttataacccc accaataaat gtgtctacta 8341 aggaaatttt caatgattat gatcgtttaa tttctaagga aaatagtgat ttaaattatc 8401 gaactaatat atataaaaag caaaaaaata atgaattaat ggaaaatgac ttgatggagg 8461 taacctttaa taagcatcca atcttggaaa aaatgaaaag attcttagaa tcattatcta 8521 aaaggaaagt aatgatgagt ggtagtggtc ctagtttatt ttgcttattt gatagtttaa 8581 atgaagctaa tcaaattaaa gatcaatatc aagaaagata cccaaatcac tatattaatg 8641 ttacccaaat tatcaatgtt gaaaaatagt taaaaaaagt atggaggtta atatggaagt 8701 aaaatttact caattggtcg gaaaagaaga tatttataat tataatatgt tctttttcac 8761 aaaaaaatct aagatgatgt actttagcta tatcgtcggt ttcttatcac taggattagg 8821 aatttactat cttattaaag gtgaaatacc agtaggcatc atttgtatcc tgttgtttgc 8881 ctattcagta ttcttacatc gtttatttac caaatggcaa attagaaaaa tgattgatcg 8941 aaagactgat atcgataata tgacagtctt tgttgaaatc gatgaagaag gcttcaccta 9001 tagtaacgaa gaagaaaaca ccggtaataa attattgtgg gataatgttt tagaactata 9061 tgattataaa aactattggt atatctatta tgcaccaaat cgtgctgttt ttgttcgaaa 9121 atctgattgt cctaatgtaa attcggttga aacaatgcta aaagaaaaat tgtcataccg 9181 ttacaaaaca gtaaaagctc gtgattaacg agcttatttt ttataaaatt aaagaaaaat 9241 ataaaaaaat gtaaaaaaac gatataattt attgcaaaaa gcgttttcat gtggtataat 9301 atggatgatc gaccttgaaa ggcaggtatt gttatggaaa ttacagacgt aaggatcaga 9361 aaaattgaag gccaaactag attaaaagct atcgcatcaa ttacaattga caacgctttt 9421 gcggttcatg aattgagaat tattgaaggc ggaaatggtt attttgtagc gatgcctagt 9481 cgaaagacac aaaacaacgt gttcagagat attgcgcatc caatcaattc agaaacgcgt 9541 caaatgatcg aaaaggtcgt ccttgacaag tttaacgaag ctgaatagga aaaggctaaa 9601 aatgctaggc aactagcgtt tttgcttgta tacactccct tatgggagtt ttttatttat 9661 tattatttcc attttaattt ttaaaaggag tcaaatagtg tataatattg ttagaggtga 9721 aaatatgcaa tcattacaaa aagtagcaaa agaaattagg aagttgactg cgcgcacaat 9781 tgcatcaatt ggagccggtc acataggcgg tagtttatcc atcgttgatg tcctaaccgt 9841 tttgtatttt aaacatatga atgtcgaccc taaaaaccca aaaatggcag ggcgtgaccg 9901 gttaattgtg tcaaaaggtc atgctggtcc aggtgtttat gctacccttg cttataaggg 9961 attcttccca attgaggaat tagatacctt aaataagata ggcactaact tacctagcca 10021 ttgcgatatg aatcgaacta aaggtattga tatgacaact ggctctttag gccaaggaat 10081 atcttgtgca gttgggattg cgattgcatc cagacttgct agagacaatg ctcatatata 10141 tgcaattgtt ggtgatggcg aaagtcaaga aggtcaagtg tgggaagctg caatgtatgc 10201 cagccaacaa aagcttgata atttaattgt ctttttagat tataataaat atcaattaga 10261 tggtagtact tgtgatatta atgatttagg taatgtcgtt gataagtgga agagtttcgg 10321 atttaacact gtatatatcg atggtcacaa tttagaagaa atcgataagg caatagttaa 10381 cgctaaagct catactggta gtccgagtat gattatctta aataccgtaa aaggtaaagg 10441 tattagtttt gctgaaggtg ctggagccga ttctcacagt atgccaatta gtaaagatca 10501 acttcaacaa gcctataagg aattagaata ggagggacaa ctatggaaat gagacaaatg 10561 ttggctaaaa cattagccga aattatggag aagaacgata agattgtagt tatagatgcc 10621 gatttatcca aaccaaacgg aacttctgtt ctatattcaa agtttccaga tcgttgtttt 10681 aatgttggaa ttgctgaaca aaatatgatg ggaatagctg ctgggattgc tagttatggt 10741 tatattcctg tagttgtaac ttttacacct tttgcaacta gacgtccttg tgaccaatta 10801 acagtatcgg ttgcttatgc aaagcaaaat gttaaaataa ttggaactga cccaggctta 10861 actgctgaac ttaatggtgg tacacatatg agtgttgagg atgttggtat tgctcgtagt 10921 attcctgaaa tactaatcta tgatgcggtt gatccacttc aattaggaca agctttacct 10981 caaataattg attacgaggg ggctgcatat gtaagaatgc cacgtaaata tgctgagagt 11041 gtactcgatg aaaattataa atttgactta tttaaagctg acatcttaag agaaggagaa 11101 gacataactg tatttgctac aagcattatg gttaaagaat cgctagtagc agctgaaatc 11161 ttggctcaag aaggaattga tgttgaagta gtcaacgttc atactttcaa gccgttagat 11221 gaagaaacaa ttattaatag tttaaagaag actaaaaaag ccatcgtttg tgaaaaccac 11281 aatataattg gtggactatt ctcagccatc agtgaattgg tagttcaaaa atatccggtt 11341 ccgattcgac cacttggaat taaagatcgt ttcggccaag tcggaaaact accagaatta 11401 atgaaagaat acgaaatgac tgcagatgat gttgtaaaac tagttaaatc aatgttttaa 11461 taaaaaagag ggaaaagagt atgaataaaa aagggaaatt cttttggatt ttaataggaa 11521 ttggtctatt tattatcttt gtcttaatga ttgttagtag tgtaatagag gttggagaac 11581 gtttaagaag ttttggacaa gtagttgaaa ttacctttta tgtgctatgt gcttttgtct 11641 tttatttctt aatagttaac ccaattagaa ttattttgtt ttctccatct tttagcattc 11701 aaacaacctt tgataaagat aatggtaaga gcaaaaaagt atacaaaaaa gtggcactaa 11761 acatgcttaa aaatcctgat ttaagtgaag aggataaaga gttattaaaa gaaggattga 11821 aagaccgcac tgaattaaaa catcaattac aacatgtctt tgatcatagt attaaaaata 11881 gtttaaataa aataataata aagaatgcca aaacagtaat ggtgtcaacc gccatttcgc 11941 aaaatggtcg gttagattta tttactgtta tggcagttaa tattaaaatg attaaagaat 12001 tagtggtgaa atgtggcttc cgtccatctt ttaaaaatct tggaaaactc acagcaaacg 12061 tctttgtgac ggctttgatt gctgaaggat tggaagatct agactttaat gagattttcc 12121 cgaatagtac aaaaaacttt ttatcggaca tcccttttat taaacctatt gctagttcaa 12181 ttatgcaagg tatatctaat gcattactga cgattagagt tgggatagta accagaaaat 12241 acttattctc tgatggaaaa gaaataacaa aacaagaagt aagaaaatat gctctcaaag 12301 aatcacttaa aatattgcca gtagttatta aacaaagcct tagtgttttc ccggatcgaa 12361 ttaaaaagat gttcactccc aaaaaacatg caaatgaagc tgaaagcaat tagcgttaaa 12421 ccctatatta aataaatatt aagaaagatg gtggatccta atggcaatta ttaatggagt 12481 aaaagtaaaa atctttactc tgaattctaa ccctgctctt gccaaagaga ttgctgatta 12541 tgtagggata gaattatcaa aatgcgaaat ttctcgattt gcagacggtg aagttcaaat 12601 cgatatccct gagaccgttc gtggacacag tgtgtttgtt gttcaatcaa caaataaacc 12661 agttaacgaa aactacatgg aacttttaat tatgattgat gcccttaaac gagcatcagc 12721 acgatcaatt aatgttatta tgccgtatta tggctactca cgccaagacc gaaaagcagc 12781 tgctcgccaa ccgatctcag caaaacttgt tgctgactta ctacaggctg caggagctaa 12841 tcgggttatc tgcatggatt tacacgctga tcagatccaa ggtttcttta atataccaat 12901 tgataacttt gtggcgttac caatattggc ggactatttt gttcaacaag aaattaaaga 12961 tattgtagtt gtatcaccag accatggtgg aacaaccaga gccagaagac ttgctaatgt 13021 ttttgatgct ccgattgcca ttgttgataa gcgaagaccg aagccgaacg tggccgaagt 13081 aatgaatatt attggggatg ttaagggtaa aaatgcgatc attatcgatg acatgattga 13141 cactgcggga actatcacac ttgcagccac tgctctcaaa aaatttgggg ctaaagaagt 13201 ttatgcatgt tgtactcatc cgatcttatc agatcctgct attgaaagat tggctaactc 13261 tgatataaag gaagtaataa caactaatac aattgctttg gatgaaacca aaaagataag 13321 taaaatcaaa caactatcag ttgcaaaatt atttggtcaa ggaatcttaa atataataga 13381 tgaccgtcca gtcagtgatt tatttagata taatcacaaa cattaaaaat ctttgcataa 13441 aataaagaaa tttggaaacc ttaaaaaata gggtttaaaa attaacgggg gaaagagtat 13501 ggtatgtaaa agatgtaaaa cagaaatcaa tggtgctctt caagattgcc ctttctgtga 13561 aattccaatt gtggaataca aaataaaaaa agtaattact agaagaaatg ttgttgttga 13621 cacatcaatt ataaaaactt ataaactagc atcaaaaatg ttgaagaaga tatctagtaa 13681 tgagactgcc tataaaatca actgttacaa agatgcatat ttcttaaaca tttagtaacc 13741 atttgcttta attattaata taataataag caagtgaaaa taaaggacaa aaaaagttca 13801 tttttacttg acacagaaag cacaaagtag tataatatac aaggttgcgc aaaatctgtg 13861 caatcttgta tttttaatat acacacaaat cataatccca taaataggta aataaaaaat 13921 gctgaatttc ttgacaaaag aaaacctatt tagtataata tagcatgctg aatatgattt 13981 ttagcaaaac tgattatttt ggattaatca aggtctttga aaactgaaca gaacaggtgg 14041 agcaggtaaa actttttatt gagagtttga tcctggctca ggattaacgc tggcggcatg 14101 cctaatacat gcaagtcgaa cgagagtagc aatac //