LOCUS DUMN01000220 5294 bp DNA linear ENV 25-APR-2020 DEFINITION MAG TPA_asm: Brucella intermedia isolate AS04akNAM_66 22191_AS04, whole genome shotgun sequence. ACCESSION DUMN01000220 DUMN01000000 VERSION DUMN01000220.1 DBLINK BioProject: PRJNA602310 BioSample: SAMN13893596 Sequence Read Archive: DRR066655, DRR066656, DRR066657, DRR066658, DRR066659, DRR066660, DRR066661, DRR066662, DRR066663, DRR066664, DRR066665, DRR066666, DRR066667, DRR066668 KEYWORDS WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA; TPA:assembly. SOURCE Brucella intermedia (anaerobic digester metagenome) ORGANISM Brucella intermedia Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Brucellaceae; Brucella/Ochrobactrum group; Brucella. REFERENCE 1 (bases 1 to 5294) AUTHORS Campanaro,S., Treu,L., Rodriguez-R,L.M., Kovalovszki,A., Ziels,R.M., Maus,I., Zhu,X., Kougias,P.G., Basile,A., Luo,G., Schluter,A., Konstantinidis,K.T. and Angelidaki,I. TITLE New insights from the biogas microbiome by comprehensive genome-resolved metagenomics of nearly 1600 species originating from multiple anaerobic digesters JOURNAL Biotechnol Biofuels 13, 25 (2020) PUBMED 32123542 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 5294) AUTHORS Campanaro,S. TITLE Direct Submission JOURNAL Submitted (28-JAN-2020) Department of Environmental Engineering, Technical University of Denmark, Bygningstorvet, Bygning 115, Kgs. Lyngby 2800, Denmark COMMENT The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Assembly-Data-START## Assembly Date :: JUN-2018 Assembly Method :: Megahit v. 1.1.1 Genome Representation :: Full Expected Final Version :: No Genome Coverage :: 114.443644783x Sequencing Technology :: Illumina HiSeq2000 ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Date :: 04/10/2020 17:18:30 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Method :: Best-placed reference protein set; GeneMarkS-2+ Annotation Software revision :: 4.11 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total) :: 5,125 CDSs (total) :: 5,077 Genes (coding) :: 4,953 CDSs (with protein) :: 4,953 Genes (RNA) :: 48 tRNAs :: 44 ncRNAs :: 4 Pseudo Genes (total) :: 124 CDSs (without protein) :: 124 Pseudo Genes (ambiguous residues) :: 0 of 124 Pseudo Genes (frameshifted) :: 50 of 124 Pseudo Genes (incomplete) :: 75 of 124 Pseudo Genes (internal stop) :: 17 of 124 Pseudo Genes (multiple problems) :: 18 of 124 ##Genome-Annotation-Data-END## FEATURES Location/Qualifiers source 1..5294 /organism="Brucella intermedia" /mol_type="genomic DNA" /submitter_seqid="22191_AS04" /isolate="AS04akNAM_66" /isolation_source="anaerobic digestion of organic wastes under variable temperature conditions and feedstocks" /db_xref="taxon:94625" /environmental_sample /metagenome_source="anaerobic digester metagenome" /note="metagenomic" gene 371..1792 /locus_tag="GXX48_07530" CDS 371..1792 /locus_tag="GXX48_07530" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_006471384.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="diguanylate cyclase" /protein_id="HHV67479.1" /translation="MDHSASKIEATWGQKTGLGTSQIEWIELVGGLPEQDSLFLRDLI GRHREQFVGVFYDRLMSNRDAATFLSQKMVQERLHNSLSDWLLKMFERTADDVDAFIR EQRKVGEVHARIDVPIHVVMQGARLLKNEIIRQLKLEVATREGLARLIVHVSNAIDIA IEIMSQSFVKDARESAQTDEAYRAFTLSHDFPLERESQRAALMEWSQSLLFNLYGNPD SPLHTLASSDFGLWLQHKADLMFSNSSTLENIRRNVEHIDNVLLPAINGARKDNPTEL AASLSLFQTKIGEVKFLLSELFQAAAAMETGRDPLTRALNRRFMPSILTREVRMASQK AMDLSVLMIDIDHFKNINDTYGHSSGDAVLQQVAETILSLCRPSDFVFRYGGEEFLVA LVEADEDKAFAIGEELRRQIAQQPMNLTDGARQSVTLSIGVAAFNGHPDYSHLVNAAD RALYRAKQAGRNRCEKEVPLAID" gene 1851..2618 /gene="lipB" /locus_tag="GXX48_07535" CDS 1851..2618 /gene="lipB" /locus_tag="GXX48_07535" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_002972950.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="lipoyl(octanoyl) transferase LipB" /protein_id="HHV67480.1" /translation="MTNSSVFPVSEIALPKPRERDALETRFLPQQADAPPVDWVVADG LTDYEEALSFMEARVQAIREGTANELVWLVEHPSLYTAGTSASASDLLAPDSLPVFNT GRGGEYTYHGPGQRVAYVMLDLKRRREDVRAFVTALEQWIIESLAEFNIKGERREDRV GVWVTRPEKPRLANGGMCEDKIAAIGIRLRRWVSFHGIAINVEPDLSHYSGIVPCGIS EHGVTSLVDLGLPVTMGDMDVALGKAFETVFGPRQLK" gene complement(2625..3554) /locus_tag="GXX48_07540" CDS complement(2625..3554) /locus_tag="GXX48_07540" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_006468924.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="EamA family transporter" /protein_id="HHV67481.1" /translation="MQSSATGGMEAHVKGMAIMVLSVLLLPLMDAIGKWLAMADHMPP ATVTFMRFFVQCWLMFFILLMVGGFAALRTQHLAGNLTRGALMGFGGLCFFTAVKYMP LADAMAVFFAEPLILTLFSAIFLKETVGWRRFSAVGIGLIGTMIVIQPSFEIFGAVSL LPLATAVTFAIYLILNRKYGAKESPLVMQFYAGVGGWMFAGLAMIYGTAAGLGDLSFG LPQGIQPWLLLLLLGTIGTVSHLMVVQAFKLAPASMLAPFQYLEIVNAVLVGLIVFGD FPTPSKWFGIAIIVGSGFYVFMRERRVKADAVA" gene 3862..>5294 /locus_tag="GXX48_07545" CDS 3862..>5294 /locus_tag="GXX48_07545" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_002966042.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="DNA topoisomerase IV subunit B" /protein_id="HHV67482.1" /translation="MDDSNDLFSRVVNNARAREQEQGRQSEKPVAPQPVAQPVAAPSL SVPKPVTAKAPPASSAGDDYNASSIRVLEGLEPVRLRPGMYIGGTDEKALHHLFAEVI DNSMDEAVAGHANFIEVSFDADGFVTVSDNGRGIPVDEHPQVPGKSTLEVIMTKLHAG GKFDGKAYETSGGLHGVGVSVVNALSDVLEVEVARNRRLYRQRFSRGIPQGGLEDVGE VHNRRGTRVRFHPDPDIFGKTAQFDPHRLYRMARSKAYLFGGVEIRWNCDPSLLDPKK ETPEKAVFHFPGGLKDYLEASLGKEHQVTREVFAGKTEKQGGHGAVEWAVSWFGGDGF VHSYCNTIPTGDGGTHEAGLRIALTRGLKAYAELTNNKRASIITTDDVMVSAAGMLSV FIREPEFVGQTKDKLATVEAQRIVENAIRDPFDHWLTASPQEASRLLEWVIDRADERL RRRQEKETVRKSATRKLRLPGKLADCT" BASE COUNT 1201 a 1522 c 1502 g 1069 t ORIGIN 1 agttagcgcg aaaagcacga gagccgcaga aaccaacttg aatctcattc cattttcctt 61 ttcagagctg tccagaattg gaatatcttg cccacaaata cctaggtcaa acgggcgagt 121 ggtaaagtca cttaatctta cggtgagcta tcaatctcga ccctgcccca tcaaacgggc 181 cgccgcacag gacctgcaat tttgctgggg tatttttaag agaatctgga tatcagaagc 241 caattgaatt cgcatgctat cccttgaagc aagggagaag ctgcagaatc tccaatctgc 301 aaagccgatc ctgcgccggt tcacgtccgg taacgggcgg agctttgcgg ctgtcgcaag 361 gagcaggaac atggatcatt cggcctctaa aatcgaagcc acatggggcc agaaaaccgg 421 tctgggtacg tcccagatcg aatggatcga actggtcggc ggcttgccgg agcaagattc 481 gctcttcctt cgcgacctga tcggacggca ccgtgagcag ttcgtcggtg tgttctatga 541 tcgtctgatg agcaatcgtg atgcggcaac tttcctgtcc cagaaaatgg tccaggagcg 601 cctgcacaac tcgctgagcg actggcttct gaaaatgttc gaacgaaccg ccgacgatgt 661 tgacgcattc attcgtgagc agcgcaaggt cggcgaagtt catgcccgca tcgacgtgcc 721 gatccatgtc gtcatgcagg gcgcgcgcct gctcaagaac gagatcattc gtcagttgaa 781 gctggaagtt gcaacacgcg agggtctggc gcgtctgatc gtgcatgtca gcaatgcaat 841 cgacattgcg attgaaatca tgagccagtc cttcgtgaaa gacgcgcgcg aaagtgccca 901 gaccgacgaa gcctatcggg cctttacgct gagccatgat tttccgcttg agcgagaatc 961 gcaacgtgcc gcactcatgg aatggagcca gtcgctcctt ttcaatctct atggcaatcc 1021 cgacagcccg cttcacacgc tggccagctc ggacttcgga ttgtggttgc agcacaaggc 1081 cgatctcatg ttctccaatt cctcgacgct ggaaaatatc cgccggaatg tagaacatat 1141 agacaatgtg ctccttccgg ccatcaacgg cgcccgcaag gataacccga ctgaactcgc 1201 tgcctccttg tcactgttcc agacaaagat cggggaagtg aagtttctgc tatccgaact 1261 ttttcaggcg gctgcggcca tggaaacggg ccgcgatccg ctgacccgcg cactcaatcg 1321 tcgtttcatg ccctcaatcc tgacccgcga agtgcgcatg gcgtcccaaa aggcaatgga 1381 tcttagcgtt ctgatgatcg atatcgatca cttcaagaac atcaacgaca cctacgggca 1441 cagcagtggc gatgcggttc ttcagcaggt ggccgaaacg atcctttcgc tctgccgtcc 1501 aagtgatttc gtgttccgct atggcgggga ggaatttctg gtcgcgcttg tcgaagccga 1561 cgaggacaag gcatttgcaa tcggcgagga actgcgcaga cagatagccc aacaacctat 1621 gaacctgacc gatggcgcac gtcagtctgt caccctgtca atcggtgttg cggcattcaa 1681 cggccatccg gattacagcc acctcgtgaa cgctgctgac cgggcgctct atcgcgccaa 1741 gcaagccggt cgcaatcgct gtgaaaagga agtgccgctc gcgattgatt gattgcagag 1801 gcattttgac atctggcggt tacgctgcaa aaccgccata tgctcccatc atgacgaata 1861 gctccgtttt ccccgtttcc gaaattgcct taccaaaacc gcgcgagcgc gacgcgttgg 1921 agacgcgttt cctgccgcaa caggcagatg caccgcctgt ggactgggtt gtcgcagacg 1981 ggctgacgga ctacgaggag gcactttcct tcatggaagc gcgtgtgcag gccattcggg 2041 aaggcactgc gaacgaactc gtttggctgg tcgaacatcc ctcgctttac acggcaggaa 2101 caagcgccag tgcaagcgac ctgctggcgc ccgacagtct gcccgtgttc aacacggggc 2161 gcggcgggga atatacctat cacgggccag gccagcgtgt tgcctatgtg atgctggacc 2221 tgaaacgccg ccgcgaggat gtgcgtgcct tcgtgacggc gctcgaacaa tggatcatcg 2281 agagcctggc cgagttcaac atcaaaggcg aacggcgaga ggatcgcgtt ggcgtatggg 2341 taacgcggcc tgaaaaaccc cgcctggcca atggcggcat gtgcgaagac aagatcgcgg 2401 ctatcggcat ccgtctgcgg cgctgggtga gcttccacgg catagccatc aatgtcgaac 2461 ctgatctcag ccattattcc ggcatcgtgc cttgcggcat ctccgaacat ggcgtcacca 2521 gccttgtcga tctggggttg ccggtaacga tgggcgacat ggacgtcgct ttgggaaagg 2581 cgttcgagac ggtattcggt ccgcgtcagc tcaaatagtt ctgctcaggc cactgcgtcg 2641 gccttcaccc ggcgctcgcg catgaaaaca tagaaacccg atccgacgat gattgcgata 2701 ccgaaccatt tcgatggcgt cgggaaatcg ccgaatacga tcaggccaac cagaaccgca 2761 ttgacgattt caagatactg gaacggcgcc agcatcgatg ccggtgcgag cttgaacgcc 2821 tgcacgacca tgaggtggct gacagtgccg atcgttccca gaagcaacag gagcagccag 2881 ggctggatgc cctgcggcag cccgaaactc aggtcgccaa gccccgccgc agttccatag 2941 atcatcgcga gccccgcaaa catccagccg cccacaccgg cataaaactg catgacaagc 3001 gggctttcct tcgcaccgta tttgcggttc aaaatgagat agatcgcaaa ggttacggcg 3061 gtcgcgagcg gcagaagcga gaccgcgccg aaaatctcga aactcggctg gattactatc 3121 atcgtcccga tgaggccgat accgacagcg ctgaatcttc tccagcctac tgtctccttc 3181 aggaaaatgg ccgaaaacag cgtcagaata agcggctcgg caaagaagac ggccatcgca 3241 tcggccagcg gcatatactt gactgccgta aagaagcaga gcccgccaaa ccccatcagc 3301 gcaccacgag tgaggttgcc tgccagatgc tgcgttcgca acgctgcaaa gccgccgacc 3361 atgagcagga tgaagaacat cagccagcac tgaacgaaga agcgcatgaa ggtcacggtt 3421 gccggaggca tatgatctgc catggcgagc cacttgccga tggcatccat cagcggcagc 3481 agaagcaccg agagcaccat gatcgccatg ccttttacgt gcgcttccat gccgcctgtc 3541 gcgctgctct gcatttcaaa ctctcggtat ctggaatgtc ccgctatggc tctatgcgaa 3601 ctgccgcagc gtttccatcc aatttgaccg gaattgcccc gcttgtttga ctttcgaccg 3661 ctttctggtg caaatcagtt ccgttctgga caaaaccctt tgaaaaagcc gtgcgcaaac 3721 ggaacgtaag gcgaacttta gtcgcctttc gcgagcgcgc gtggcatcat tcaagtgatt 3781 cgcgaacagt attttccggt ctttggcgag caggctcgac agcggcctcc tgaaattcag 3841 atagagagct tttcggaaca aatggacgat agcaacgatc tcttctcgag agtggtcaac 3901 aatgcccggg cccgagaaca ggaacagggg cgacagagcg aaaaacctgt ggcgccgcaa 3961 cctgttgcgc agccggtggc agcgccgtcg ctctctgttc caaagccggt aacggccaag 4021 gcgccgcctg cttcatcggc aggcgatgac tataatgctt cgtccatccg cgttctggag 4081 ggtctggaac ccgtacgcct gcgccccggc atgtatattg gcggcacgga cgaaaaggcg 4141 ctgcatcacc tctttgcgga agtcatcgac aactcgatgg acgaagcggt ggcaggacat 4201 gccaatttca tcgaagtcag tttcgatgcc gacggatttg tcaccgtctc ggacaatggg 4261 cgcggcattc cagtggacga acatcctcag gttccgggaa aatccacgct cgaagtcatc 4321 atgaccaagc tgcatgctgg cggtaaattc gacggcaagg cttatgaaac gtcaggcggt 4381 ctgcatggcg tgggtgtttc ggtggtgaat gccctttcgg acgtgcttga agtggaagtg 4441 gcacgcaatc gccgtcttta tcggcagcgt ttctcgcgcg gtattccgca gggcggactg 4501 gaagatgtcg gcgaagtgca taaccgtcgc ggcacccgtg tccgtttcca cccggaccct 4561 gatatcttcg gcaaaacggc gcagttcgat ccgcatcgtc tctatcgcat ggcgcgatca 4621 aaagcctatc tgttcggcgg ggttgaaatc cgctggaact gtgatccttc cctcctcgac 4681 ccgaagaagg agacgcctga aaaggcggtg ttccacttcc ccggcggcct gaaagactat 4741 ctggaggcat cgctcggcaa ggaacatcag gtcacgcgtg aggttttcgc aggcaagacc 4801 gaaaagcagg gtggccacgg cgcagttgaa tgggccgttt catggtttgg cggcgacggt 4861 ttcgtccact cctattgcaa caccatcccg accggcgatg gtggcaccca tgaggcaggc 4921 ctgcgcattg cgctgacacg cggcctgaaa gcctatgcgg aactgaccaa caataaacgc 4981 gcctcgatca ttaccactga cgacgtgatg gtgtcggcgg cgggaatgct gtcggtcttc 5041 atccgcgagc cggaatttgt tggccagacc aaggacaagc tggcgaccgt cgaagcgcag 5101 cgcatcgtgg aaaatgcaat ccgtgatccg ttcgatcact ggctgacggc ttctccgcag 5161 gaggcgtctc gccttctgga atgggtgatc gaccgcgcgg atgagcgttt gcgtcgccgt 5221 caggaaaagg aaactgtccg caaaagcgcg acgcgcaagc tgcgcctgcc gggcaagctt 5281 gccgattgca ccca //