LOCUS DUGL01000145 12343 bp DNA linear ENV 11-JUN-2020 DEFINITION TPA_asm: Methanoregulaceae archaeon isolate UBA10516 contig_8490, whole genome shotgun sequence. ACCESSION DUGL01000145 DUGL01000000 VERSION DUGL01000145.1 DBLINK BioProject: PRJNA593905 BioSample: SAMN13192961 Sequence Read Archive: SRR1313281, SRR1313282, SRR1313285, SRR1313283, SRR1313287, SRR1313284 KEYWORDS WGS; Third Party Data; TPA; TPA:assembly. SOURCE Methanoregulaceae archaeon (hydrocarbon metagenome) ORGANISM Methanoregulaceae archaeon Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia; Methanomicrobiales; Methanoregulaceae. REFERENCE 1 (bases 1 to 12343) AUTHORS Rinke,C., Chuvochina,M., Mussig,A.J., Chaumeil,P.-A., Waite,D.W., Whitman,W.B., Parks,D.H. and Hugenholtz,P. TITLE A rank-normalized archaeal taxonomy based on genome phylogeny resolves widespread incomplete and uneven classifications JOURNAL bioRxiv, doi 10.1101/2020.03.01.972265 (2020) REFERENCE 2 (bases 1 to 12343) AUTHORS Rinke,C. TITLE Direct Submission JOURNAL Submitted (20-FEB-2020) Australian Centre for Ecogenomics, School of Chemistry and Molecular Biosciences, The University of Queensland, Cooper Road, Brisbane, QLD 4072, Australia COMMENT The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Assembly-Data-START## Assembly Method :: CLC NGS Cell v. 4.4.1 Genome Representation :: Full Expected Final Version :: Yes Genome Coverage :: 0.51x Sequencing Technology :: Illumina HiSeq 2000 ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Date :: 03/15/2020 22:19:13 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Method :: Best-placed reference protein set; GeneMarkS-2+ Annotation Software revision :: 4.11 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total) :: 1,888 CDSs (total) :: 1,860 Genes (coding) :: 1,777 CDSs (with protein) :: 1,777 Genes (RNA) :: 28 tRNAs :: 27 ncRNAs :: 1 Pseudo Genes (total) :: 83 CDSs (without protein) :: 83 Pseudo Genes (ambiguous residues) :: 33 of 83 Pseudo Genes (frameshifted) :: 48 of 83 Pseudo Genes (incomplete) :: 19 of 83 Pseudo Genes (internal stop) :: 2 of 83 Pseudo Genes (multiple problems) :: 19 of 83 ##Genome-Annotation-Data-END## FEATURES Location/Qualifiers source 1..12343 /organism="Methanoregulaceae archaeon" /mol_type="genomic DNA" /submitter_seqid="contig_8490" /isolate="UBA10516" /isolation_source="hydrocarbon" /db_xref="taxon:2485498" /environmental_sample /geo_loc_name="Canada: North Alberta" /lat_lon="57.02 N 111.55 W" /metagenome_source="hydrocarbon metagenome" /note="metagenomic" gene 103..507 /locus_tag="HA256_05575" CDS 103..507 /locus_tag="HA256_05575" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_011306519.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="pyridoxamine 5-phosphate oxidase" /protein_id="HIH27432.1" /translation="MVMLTAEMKETFGKIKLFPVATASKSGVPNVAPIAFVMLVSDDT IWLADNYMNKTLANVKENPQIAIYLWEPETKKCFQIKGKVEVRTSGPDFEKMQAMVKG KKPDAPAKSLFVMKVTEVFSCAPGPGAGKKVL" gene complement(1033..1239) /locus_tag="HA256_05580" CDS complement(1033..1239) /locus_tag="HA256_05580" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_012618374.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="histone" /protein_id="HIH27433.1" /translation="MADLPIAAIVRIAKKSGAERVGHDAATVLLEKTEAYIAQLTREA NKLAMHAGRKTLKEEDVELAAMSA" gene complement(1337..2524) /locus_tag="HA256_05585" CDS complement(1337..2524) /locus_tag="HA256_05585" /EC_number="6.3.4.5" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_011447190.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="argininosuccinate synthase" /protein_id="HIH27434.1" /translation="MGKGTVVMAYSGGLDTSICIPLLKERYDYDRVVTVAVNVGQRDE EIAVATEKGEKFADTHYTIDAREKFVNAYLFPAIRANGSYEGYPMGTALARPLIAEEV VRVALHEGAKTVAHGCTGKGNDQLRFDFIFRIAGLEVIAPIRELNLTRDWEIGYAKEH DIPVPVAREKPWSIDENLWSRSIEGGRLEDPAYHPPEEIYGWTVAPEKAPDTPEVITL DFLKGVPAGMNGDAMSGLDLIQGLNLIAGRHGIGRSNMIEDRILGLKAREIYEHPAAT VILAAHADLEHLTLTRQELAFKRMVDDKWSELAYMGLVHEPLFHDLNAFITQSQEKVS GTVDLMLYKGCCRVLGRSSPEGVYSDDLVSFDSTTLDQCHAIGVSAYYGIQARLIEER KKR" gene complement(2695..5862) /gene="carB" /locus_tag="HA256_05590" CDS complement(2695..5862) /gene="carB" /locus_tag="HA256_05590" /EC_number="6.3.5.5" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_004038405.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="carbamoyl-phosphate synthase large subunit" /protein_id="HIH27435.1" /translation="MPKNPEIRTVLLIGSGPIQIGQAAEFDFSGTQACRALREEGVRV ILVNSNPATIQTDPETADVIYVEPIRAEIIEKIIERERPDGILSGMGGQTGLNMTAEL AERGALRDVRILGTPLEAIYQGEDREKFRTLMEQIGEPVPKSMILQSIDQVDTALETV GLPAIIRPAYTLGGSGGGIAHTRDELLRIVEIGLSRSRIRQVLVEQSVVGWKEIEFEV MRDAADTCIIVCGMENVDAMGIHTGESVVVAPILTLRDDEFQLLRSASIKIIRALDVQ GGCNIQFAYKDGDYRVIEVNPRVSRSSALASKATGYPIARVAAKIAIGLRLDEITNSV TGCTPASFEPAIDYIVVKVPRWPFDKFKNADRSLTTAMKSTGEVMAIGRCVEEAFKKA IRSLDTDVYSHTNGNEIRMILSRPTDERFPTLFDAFRAGFTLTEIQNLTDIAPFFLEK IRNIVELETRLTAGPCKDDILLAKIYGFTDTELEALTGIGAAEIRNLSGDPVYKMVDT CAAEFPATTPYFYSTRGDTCEIPANNRQKVLILGSGPIRIGQGIEFDYCTVHAVKALR DEHVEVHIVNNNPETVSTDFDTSDRLFFEPMSLEDIMNILTKDSYDGVMVQFGGQNAV NLAMPIHEALQQRGMKTRILGTSPDSMDVAEDRDRFSELLRDLDIPSPPNSSAYSIEE ARTKAAAIGYPVLVRPSYVLGGRAMEIVHDEIELEGYMKEAVRVSRHHPVLIDSFLQN AIELDIDAVCDGEEVLIGGIMEHIEEAGVHSGDSACVIPTQSLSPAVLSTVREYTTRI ALGLGVVGLVNLQMAVKDDTVFILEANPRASRTVPFVSKATGIPLAKIAAKVMIGKKL RDLGVHEPRIGHTAVKEVLLPFNKIPGVDTVLSPEMKSTGEVMGIDYDFGRAYYKACI SADNELPVRGNIFISVISGQKDEVVPIARKLRSLGLSLYGTSGTVEYLSQAGIDANLV RKVQEGSPNVIDLLRRGEIRLIINTPSDKQSRQDHYQIMRAAVDYGVPYITTVQAARA AALAIEAIQKEEITIEPLAHYHHAG" gene complement(5864..6919) /gene="carA" /locus_tag="HA256_05595" CDS complement(5864..6919) /gene="carA" /locus_tag="HA256_05595" /EC_number="6.3.5.5" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_011843833.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="glutamine-hydrolyzing carbamoyl-phosphate synthase small subunit" /protein_id="HIH27436.1" /translation="MKAVLGLEDGTFVPGVGFGAEGECAGELVFTTQMSGYMEALTDP SYHGQILMFTFPTIGNYGVDHQNFQHPRVWAGGCIAREICAIPEIGPSVSDYFADCGL PGIQGVDTRMLTIKTRVQGTLRAALVVGDDDGEYAVTCARNVPPISDCDLIPAVSCTR PYHLEGSGKRVAVLDLGIKKNMILSLQARNADIHVFPHDTTPDQVAACRPDALFISNG PGDPVHAAHAIRCVSELAGTLPIYGICMGIQVCGLALGGNTYKMKFGHRGSNQPVRYK DGSIYITTQNHGFAVAADSLPEGCRVLYANVNDGSLEGFENEYLDITCVQFHPEAHGG PRDTEIPFFDTMFRRLD" gene 7189..7737 /locus_tag="HA256_05600" CDS 7189..7737 /locus_tag="HA256_05600" /EC_number="4.1.1.19" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007315338.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="arginine decarboxylase, pyruvoyl-dependent" /protein_id="HIH27437.1" /translation="MVVPSKIFFTKGAGVHRDRLASFELALRKAGIEKYNLVYVSSIF PPNCRLVTVDEGSRLLEPGDITYCVMAKNDTNEPNRLISAAIGLALPKEAQEYGYLSE HHAYGETADKTGDYAEDLAATMLATTLGIEFDINKAWQEREQIYKASGKIIKTTNICQ SAQGDKNGLWTTVIAAAVFISD" gene 7838..8536 /locus_tag="HA256_05605" CDS 7838..8536 /locus_tag="HA256_05605" /inference="COORDINATES: ab initio prediction:GeneMarkS-2+" /note="Derived by automated computational analysis using gene prediction method: GeneMarkS-2+." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="HIH27438.1" /translation="MTGTWIRVAGVLLLLVMISLSGCVEVPKGSTDATGQAGNSGDTR GGTGPGATATQAPVTGFLTPATPFPAETTPFQTMSWTRFPDESPVHVEYVALYYTVVA FRNTRTAYSYNLVYAPLVIDICIKPNMTTRTIWYEDRLGKTTERTETITTISPNAWFE VTVRDSSSGNIVAKEGFARLYSVDTAKSLVIRSPGNYLIEFTGNDITAEIQIRVQRAE NQTGASLRTLSCRP" gene complement(8507..9133) /locus_tag="HA256_05610" CDS complement(8507..9133) /locus_tag="HA256_05610" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014866746.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="molybdenum cofactor guanylyltransferase" /protein_id="HIH27439.1" /translation="MRSAIVLVGGEGRRVNGMEKYFFVFQGKTFIERLISSLEPVVDE IILVAKDEEHCERFACIDGVRCVHDIRRGIGPIGGLHAGVHASRGDLLFVCACDMPCV STRIVAALFDALGAYDAAIPSWSEDMLEPLHAVYRREPLLAYLDDHISLSLREMVKNL DATYVPVDQFRSLDPGLISFVNINKLEDMGDLLPADHPESGPAGERPE" gene complement(9399..9854) /locus_tag="HA256_05615" CDS complement(9399..9854) /locus_tag="HA256_05615" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_012106661.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="methylated-DNA--[protein]-cysteine S-methyltransferase" /protein_id="HIH27440.1" /translation="MEMNTGSARFGLWYVHVWWAGNVIYQVRFSRLLLEGPVPVTIAR YLAGKVVDLSVHEPGALPPGEIYARIYEEVRRIPYGETVTYGDIARRAGTSPRVVGQA MRRNMVPLVVPCHRVVSQSGIGGFTPDPAIKEDLLTMERKGKRRSRQTS" gene complement(9845..11851) /locus_tag="HA256_05620" CDS complement(9845..11851) /locus_tag="HA256_05620" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_015286126.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="ATP-dependent DNA helicase" /protein_id="HIH27441.1" /translation="MEKLDEYFPYASYRPHQREMLARVAQCAREGGIAMIDAPTGSGK SSVIAALLSERSGRRIFVAVRTISQLNTFIRELELIRKRKRQISYSYLIGKRSLCLLP GAGDVYRRCEGVKAISTNLMRERAEQGSLVPAKDPLITQQIRKLDPEHPVLCPYYIRS RVFVSAEGSGLRMIPSQALKAKAERVSRELARADELRDIGAETCPYEMLLLAAQRSDV VIVNYHHLFNEDIRDQLYGSLGVEPGDAMLLIDEAHNCGEVMTGIMDVRIGEAVLVQA ERELSGIRRHLKSAAAIVQIIQNIGSFMQGLRNSCETEDWFDPAIFDRMVVRGSLYAD MSGVVEDLMQISEHIREKNMMAGEYATSATETLNEFLFRISCSSTDPAYLTLYRKDDT DIVLEVRNIDPSERLRDLVTAHHCTVLISGTLSPLESYRKYYFGDLPAVTCSLPNMFP KKNRLVLAAGDITTAFSMRENRENRDRTARYITTFSQLKGNVAVYFPSYQILDTYAQM LAHPLARRNLIIEPKDARDAGEALRTFLSLPAQGKSGILFAVAGGKWSEGLDYRGELL SGAMVIGLPLAPYNRVRQMTIDYYRQRFGAEGEFISYTLPALNRAQQAIGRVLRTPEE RGVLVLGDRRFLEPRVKTGLPPWIRGELVECNAESFSGAVKRWK" gene 11908..>12343 /locus_tag="HA256_05625" CDS 11908..>12343 /locus_tag="HA256_05625" /EC_number="4.3.2.2" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_011447855.1" /note="Catalyzes two discrete reactions in the de novo synthesis of purines: the cleavage of adenylosuccinate and succinylaminoimidazole carboxamide ribotide; Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="adenylosuccinate lyase" /protein_id="HIH27442.1" /translation="MAIHPIDFRYGTPEMRAVFSEENRFLSIVQAEIALARAGADFDL IPPVAASQIKENALAASLARAKEIEEEINHDMMAIVRAISEVCGDAGGYVHYGATSND ILDTATGLQLKEALGILDAKLHCLLAVLVCRSRETRHLVCAAR" BASE COUNT 2514 a 3709 c 3497 g 2623 t ORIGIN 1 tccctgccag aagaattctc cctttcccgg ataccggcaa tcggaaagaa ataaaacagg 61 cgagtcctat tggactacta tatctgaatc caggtgatac ccatggtcat gctgactgcc 121 gaaatgaaag agacctttgg gaagatcaag ctcttcccgg tcgccaccgc atcgaagagc 181 ggggtaccaa acgttgcccc gatcgcattt gtcatgctcg tatccgatga tacgatctgg 241 cttgccgaca actacatgaa caagacgctt gccaatgtca aggagaaccc gcagatcgcc 301 atctacctct gggagccgga gacgaaaaag tgcttccaga tcaaggggaa ggtggaggtc 361 aggacgagcg ggcctgactt tgagaagatg caggcaatgg tgaaagggaa gaagcccgac 421 gccccggcaa aatcgctctt cgtgatgaag gtcaccgagg tcttttcctg tgcgcccggt 481 cccggcgccg ggaagaaagt gctgtaacac cgggagtatc cggacttccg tacctttttt 541 tacccgcccc cctgttgttt ttgagaagag ggattctcgc ccacacaaga gagcgaagag 601 cgattttttc tgacaccact gttcccccct tccccgtggc aacgctcatg gcgctggaac 661 cccgcgagcc cttatgtccg ctgttcagca cgggaaagcg taacctggcc ggaatacgtg 721 cgaatgggcc gctggttgcg agccctgccc ccccatgaaa ctccaatgct ccggaagggg 781 tttcataccg gcggtggatc gcatctccct cacctctgtt attcgccagg agttcccccc 841 taagagacag cgaaacagat catcctgtca ggatactcca catctcccgc gcgattgcag 901 tcctggaata taatccctgc ccgcgagcag ggttacggcg gaaacgacac gggaagatcc 961 aggcaggaaa ttgccggaga agaaaggaaa gaacaggaga gttcgaatga aagacgaaag 1021 agatggggga actcaggcgg acattgccgc gagttccacg tcctcctcct tcagtgtttt 1081 tcgtcctgca tgcatcgcca gcttgtttgc ctcacgggtg agctgggcaa tgtacgcttc 1141 agtcttctcc aaaagcacgg tagccgcatc gtgtccgaca cgctctgcgc cactcttctt 1201 ggcaatccgc acgattgccg caataggtaa atctgccacc ttattacctc aatgtaatga 1261 ggaaaatagt aatatttata ccttgtgtaa tttcgggcct gttttttcag ggcctgggaa 1321 aaaggtgggc atccggttac cgctttttcc gctcctcaat gagccgtgcc tggatcccat 1381 aatatgccga caccccgatc gcatggcact gatcgagtgt cgtactgtca aaggagacca 1441 ggtcgtccga gtagactcct tcaggcgagg accgccccag cacccggcag cagcccttgt 1501 agagcatgag atccaccgtt cccgatacct tctcctggga ctgggtaatg aaagcgttca 1561 ggtcatgaaa gagcggttcg tggacgagac ccatgtacgc gagctcggac cacttgtcgt 1621 ctaccatccg cttgaaggcg agctcctggc gggtgagggt gaggtgctcg agatcggcat 1681 gggcggccag gatgacggtt gctgccgggt gctcgtagat ctcccgggct ttcaggccaa 1741 ggatgcggtc ctcgatcatg ttggaccgcc cgatcccgtg ccgcccggca atcaggttca 1801 acccctggat aaggtcaagc ccgctcatgg catctccgtt catgcctgcg ggcacccctt 1861 tcaggaagtc aagggtgatc acctcaggcg tgtccggtgc cttttccgga gcgaccgtcc 1921 acccatagat ctcctcggga ggatggtacg cagggtcttc gagccgtccg ccctcgatac 1981 tccggctcca gaggttctcg tcgatgctcc agggcttctc ccgtgctacc ggcaccggga 2041 tgtcatgctc ctttgcgtat cctatctccc agtcccgcgt caggttgagc tcgcggatcg 2101 gcgcgataac ctcgaggccg gcgatccgga agatgaaatc gaaccggagc tggtcattgc 2161 ccttgcccgt gcaaccatgc gcgaccgtct ttgccccttc atggagggcg acccgtacca 2221 cttcctctgc gatgagcgga cgggcaagag cggtgcccat cgggtaaccc tcgtaggagc 2281 cgtttgcccg gatggcgggg aagaggtacg cattcacgaa cttctcccgc gcatcgatgg 2341 tgtagtgggt atcggcgaac ttctctcctt tctctgttgc gactgcgatc tcctcgtctc 2401 tctggccgac attcacagcg acagttacga cccggtcgta gtcgtaccgc tctttcagga 2461 gcgggatgca aattgaggtg tcaaggccgc ctgaatatgc catgacaaca gttcctttac 2521 ccatttttta ttgctccgta ctctgcccgg aagggcatat tgtacctact acatgccaca 2581 tcgcggataa taatactacc agaaatctcc gggaataaca cccctcccgg gaccacccgg 2641 catacccgga tggattcgcc tgcatcatac ccttttggaa aaatgggggg atagttaccc 2701 tgcgtgatgg tagtgtgcga gcggctcgat ggtgatctct tctttctgga tcgcctcgat 2761 agcaagggct gccgcccgtg ccgcctggac cgtggtgata tagggcaccc cgtaatcgac 2821 ggctgcccgc atgatctggt agtggtcctg ccgggactgc ttgtcagacg gggtgttgat 2881 gatcagccgg atctcgccgc gccggaggag atcgatcacg ttcggcgacc cctcctgcac 2941 cttacgcacg agattcgcat cgatacctgc ctgggagagg tactcgaccg tccctgaggt 3001 cccatagagg gagagaccga gagaccggag cttccgggca atcgggacaa cctcgtcctt 3061 ctgccctgag atgaccgaga tgaagatgtt tccgcggacc ggcagctcgt tatcggccga 3121 tatgcaggcc ttgtagtacg ccctcccgaa atcataatcg atccccatga cctcgcccgt 3181 gctcttcatc tcggggctga gcaccgtgtc cacacccggg atcttgttga aggggaggag 3241 cacctctttg actgccgtat gacctatccg gggctcatgg accccgaggt cccgcagctt 3301 cttcccgatc atcaccttcg ccgcgatctt tgcgagggga ataccggtgg ctttcgagac 3361 gaacgggacc gtcctgctcg cccgcgggtt tgcctcaagg ataaagaccg tatcgtcctt 3421 taccgccatc tggaggttga cgagccccac cactccaagc cctaacgcga tccgggtggt 3481 gtattcccgt acagttgaaa ggacggccgg ggagagcgac tgggtcggta tcacgcacgc 3541 cgaatccccg gagtggactc cagcctcctc gatatgctcc atgattccac caatcagcac 3601 ctcctccccg tcacagacgg catcgatatc cagctctatg gcgttctgga ggaacgaatc 3661 aatcagtacc gggtggtgcc tgctcacccg taccgcttcc ttcatgtatc cctcaagctc 3721 gatctcgtcg tgcacgatct ccatcgcccg gccccccagc acataggacg ggcggacgag 3781 gaccgggtac ccgatcgcgg cagcctttgt ccgggcctct tcgatcgagt atgccgagct 3841 gttgggcggg ctcgggatat ccaggtcccg caggagctcg gagaaccggt cccggtcctc 3901 ggcaacatcc atcgagtcgg gagacgtgcc gagtatccgg gtcttcatac cccgctgctg 3961 cagcgcctcg tgtatcggca ttgccaggtt caccgcgttc tggccgccga actggaccat 4021 gaccccgtca tatgaatcct tcgtgaggat attcatgatg tcctcgagtg acatcggctc 4081 gaagaagagc cggtccgagg tgtcgaaatc ggttgagacc gtctcggggt tgttgttgac 4141 gatatggacc tctacgtgtt cgtccctgag cgccttcact gcatgtaccg tgcagtagtc 4201 aaactcgatc ccctgcccga tcctgatcgg accggatccc agaatgagaa ccttctgccg 4261 gttgtttgcc gggatctcgc aggtatcgcc ccgggttgag tagaaatacg gcgtggtggc 4321 aggaaattct gcagcacagg tatccaccat cttgtatacg ggatccccgg agaggttccg 4381 gatctcggct gcaccgatcc cggtcaacgc ctcgagctcg gtgtccgtga acccatagat 4441 cttggcgagg agaatgtcat ccttgcacgg accggctgta agccgggtct caagctcgac 4501 gatattcctt attttttcaa ggaagaacgg tgcaatatcg gtgagattct gtatctcggt 4561 gagggtaaat cctgccctga acgcatcgaa gagtgtcggg aaccgctcat cggtcgggcg 4621 ggagaggatc atccgtatct cgtttccgtt ggtatggctg tatacgtcgg tatcaaggga 4681 gcggatggct tttttgaacg cctcttcgac gcatcggccg atcgccatca cctcgcctgt 4741 gcttttcatt gcggtcgtga ggctccggtc cgcattcttg aacttgtcaa acggccagcg 4801 gggaaccttg accacgatgt aatcgatcgc gggttcgaac gatgccgggg tgcaccccgt 4861 gaccgagttg gtgatctcat ccagccggag tccgatcgca atttttgcag cgacccgtgc 4921 tatcgggtac ccggtggcct ttgatgcgag tgcacttgaa cgggacactc tcgggttcac 4981 ctcgatgacc cggtagtcgc cgtccttata cgcgaactgg atgttgcacc ctccctgcac 5041 atcgagggcg cggatgatct tgatggaagc gctccgcagg agctggaact cgtcgtcccg 5101 cagtgtcagg atcggcgcaa ccaccacgct ctccccggta tggatgccca tggcatcgac 5161 attctccata ccgcagacga tgatgcaggt atctgcagcg tcccgcatca cctcaaactc 5221 gatctccttc catccgacaa cgctctgttc gacgagcacc tggcggatcc gcgatcggga 5281 gagccctatc tctacaatcc gcaggagctc gtcccgggta tgcgcgatcc ctccgccgga 5341 accgccgagt gtgtatgccg gccggatgat ggccgggagc ccgaccgttt caagagcagt 5401 atcaacctgg tcgatcgact gcaggatcat gctctttggc accggctccc cgatctgctc 5461 catcagggtc cggaacttct cacggtcctc tccctggtag atcgcctcga gcggggtccc 5521 gaggatccgg acatccctga gtgcgccccg ctcggcaagc tcggcggtca tgttcaggcc 5581 ggtctggccg cccatccctg agaggatacc gtcgggtctc tcccgctcga tgatcttctc 5641 gatgatctcg gcccggatgg gctccacgta gatcacatcg gcggtctcgg gatcggtctg 5701 gatggtagca gggtttgagt tgacaaggat gacccggacc ccctcctcgc ggagcgcacg 5761 gcatgcctgt gttcccgaga agtcgaactc tgcagcctgc ccgatctgga tagggcctga 5821 accgataagg aggacggtcc tgatctccgg atttttgggc atttcagtca agcctccgga 5881 acatggtatc aaagaacgga atttccgtat cacgcgggcc gccgtgggcc tcggggtgga 5941 actggacaca ggtgatatcc aggtactcgt tctcgaatcc ctcgagcgac ccgtcgttca 6001 catttgcgta cagaacccgg cacccttcag gcagtgaatc ggcggctacc gcaaagccgt 6061 ggttctgggt ggtgatgtag atgctcccgt ccttgtacct gaccggctgg ttgctccccc 6121 ggtgcccgaa tttcatctta tacgtattcc ctcccagggc aaggccgcag acctggatcc 6181 ccatgcagat cccgtatatc gggagtgtac cggcgagctc gctcacgcag cggatggcat 6241 gtgcggcatg gaccggatcg ccgggaccat tgctgatgaa gagggcatca ggccggcagg 6301 ccgccacctg gtcgggagtg gtatcatggg gaaagacatg gatgtcagca ttccgggcct 6361 gcagggagag gatcatattc ttctttattc ccaggtcaag cacagcaacg cgtttccccg 6421 acccctccag gtgatagggt ctggtgcagg agactgccgg tatcagatcg cagtccgata 6481 ttggaggtac gtttcgtgca caggtaaccg cgtactcacc gtcatcatcc ccaacgacca 6541 gtgcggcccg gagggtaccc tggacccggg tcttgatggt cagcatcctc gtatcaaccc 6601 cctggatccc cgggagcccg caatcggcaa agtaatctga cactgacggc ccaatttcag 6661 ggatggcgca gatctcgcgc gcaatacagc cccctgccca gacccgcggg tgctggaagt 6721 tctgatggtc taccccgtag ttgccgatcg tcgggaaggt gaacatcagg atctgcccat 6781 ggtaactcgg gtcggtcagc gcctccatgt acccggacat ctgggtggta aagaccagtt 6841 cccccgcaca ttccccctct gcaccgaaac caacacctgg cacaaacgtg ccatcctcaa 6901 gacccaggac cgccttcatg attccatacc tattgggtat gattacgttt aataggtagc 6961 ggagaagcgt acaacacggg cgctgtgacc aggcagtgac tcgtgccctc aggatttctc 7021 tggctttcgt atatccggca tatgcgatta atttttgaat tcagaaatta ttttttgata 7081 aatttcaata gtatcgcgaa ttataagatt aaaaatgata tgggcgcttt tacatgcata 7141 gaatcgggaa taaaaaaagt taagttggaa tactgcacca tggttatcat ggttgtacca 7201 tcaaaaatct ttttcacgaa gggcgcaggc gtccaccggg accgtcttgc gtcgtttgag 7261 ctcgccctgc gaaaggcggg gatcgagaag tacaatctcg tctatgtctc gagcattttt 7321 cccccgaact gcagattagt aacggtcgat gaaggatcac gtttgcttga gccaggggat 7381 atcacctact gcgtcatggc aaaaaatgat acaaacgagc cgaaccgcct tatttctgcg 7441 gcaatcgggc tcgccctccc caaagaggca caggaatacg ggtacctctc cgagcaccat 7501 gcttacggcg aaacagcaga taaaaccgga gactatgccg aggaccttgc cgcaacgatg 7561 cttgcaacca cgctcgggat cgaatttgat atcaacaagg catggcagga gcgtgagcag 7621 atctacaagg cgagcggcaa gatcatcaag actacgaaca tctgccagtc agcacagggt 7681 gacaagaacg ggctctggac aacagtcata gctgctgcgg tattcatctc ggattaatgg 7741 ggctttttca cctttttctc tctgcgctat cgcctgtttg tggatcctcc cctgattgac 7801 agaaccaata aatagacagg aacaccatcc tccagtaatg acgggaacat ggatccgtgt 7861 cgccggggtc ctcctcctcc tggtgatgat atccctctcg gggtgcgttg aagtgccgaa 7921 aggaagtacc gatgccaccg ggcaggcagg gaacagcggt gatacaaggg gcggaacggg 7981 gcctggtgcc accgcgaccc aggctccggt taccgggttc cttacccccg ccaccccatt 8041 ccccgcagaa acgacccctt tccagactat gtcgtggaca cgcttccccg acgagtcccc 8101 ggtccatgtt gagtatgttg ctctctacta tacggtagtc gcgttccgga atacccggac 8161 tgcatacagc tacaaccttg tctatgcgcc acttgtgatc gatatctgca tcaaaccgaa 8221 catgaccacc cggaccatct ggtatgagga ccggttgggg aaaacgacgg aacgcaccga 8281 gaccatcacc acgataagcc caaacgcctg gttcgaggtg actgttcgtg acagttcaag 8341 cgggaatatc gttgcgaagg aagggtttgc ccggctctac agcgtggata ccgcaaaatc 8401 cctggttatc aggagtccgg ggaactacct gatcgagttt accgggaatg atatcacggc 8461 cgagatccag atccgggtcc aacgggcaga gaaccagacg ggcgcttcac tccggacgct 8521 ctcctgcagg ccctgattca gggtgatcag ctggaaggag atcccccata tcctcgagct 8581 tgttgatatt gacaaaactg atcaggcccg ggtcaaggga tcggaactgg tccaccggaa 8641 cataggtggc atcgagattc ttcaccattt cacggaggga gagcgagatg tgatcgtcga 8701 gatatgccag gaggggctcg cgccggtaca ccgcatggag cggctcaagc atatcctcac 8761 tccacgaggg gattgcagca tcgtacgcac ccagtgcatc aaacagggct gcaacaatcc 8821 gggtgctgac gcagggcata tcacaggcgc agacgaagag caggtcgccc ctgctggcat 8881 ggactccggc gtgaagtccg ccaatcgggc cgatacccct cctgatgtca tggacacacc 8941 gcaccccgtc aatacaggca aaccgctcgc agtgctcctc atcctttgct acgaggataa 9001 tctcgtctac aaccggttcc agggaactga tgaggcgctc gatgaaggtc tttccctgga 9061 agacgaagaa gtacttctcc ataccgttca cccggcgtcc ctccccgccg accagtacga 9121 ttgcagacct catagccccc attctccctc tcctctcacc ggatgtccct cccctggtcg 9181 tgcctgaacc cccatggccg ggtcacgctg ctgccccggg gttcatgctg acccctgcca 9241 catcccgggc cgctccccgg gatcagggtg gcccctggta tgattcctgc aggccgcaaa 9301 gcgttcatag ggtcgtttgt tcaatccctg taccacagcc cgatgcagcc actcacgtgt 9361 ggcggtaaaa cgtctggtaa tccgtcctct tatcagggtt aggaggtttg tctacttcgt 9421 ctttttccct tgcgttccat ggtgagcagg tcctccttta tcgcggggtc cggggtgaac 9481 cccccgatcc ccgattgcga gacaacccga tggcagggta ccacgagggg gaccatgttc 9541 ctccgcatcg cctggccgac cacccgcggg cttgtccccg cccgcctggc aatatcccca 9601 taggtcacgg tctctccgta ggggatgcgc cggacttctt cgtagatcct ggcatatatc 9661 tcgccgggag gcagggcccc tggttcatgt actgagaggt ctaccacctt cccggcaagg 9721 taccgggcga tcgtgaccgg gaccggaccc tcgagcagta atcgtgagaa ccgcacctgg 9781 tagatcacgt tgcccgccca ccagacatgg acataccaga gcccgaaacg ggccgacccg 9841 gtattcattt ccatcgtttc accgccccgg aaaaggattc agcgttacat tccacgagct 9901 ccccgcgtat ccagggcgga aggccggtct ttaccctcgg ctcgaggaac cgtcggtccc 9961 cgagcacgag gacgccccgc tcttccgggg tgcgaagcac cctcccgatg gcctgctggg 10021 cccggttcag ggcagggagg gtgtagctga taaactcgcc ttccgccccg aatcgctgcc 10081 ggtagtagtc gatggtcatc tgcctgaccc ggttgtaggg tgcgagcggg agaccgatca 10141 ccatcgcccc ggagaggagc tcgccccggt aatcgaggcc ttcgctccac ttgccgccag 10201 cgacggcaaa gaggatcccg gattttccct gggccgggag agaaaggaag gtgcgcagtg 10261 cctccccggc atcccgtgcg tctttcggct ctatgataag gttcctccgt gcaagggggt 10321 gggcaagcat ctgcgcgtac gtatccagga tctggtagga cggaaaatag acggcaacat 10381 tccctttcag ctgggagaac gtcgttatgt agcgggcagt gcggtcccgg ttctcacggt 10441 tctcccgcat ggagaacgcg gtcgtgatgt ccccggcggc gagaaccagt cggttttttt 10501 tcgggaacat gttcgggagc gagcaggtca cggcaggaag atcgccaaaa tagtacttcc 10561 ggtaactctc caacggtgag agcgtgcccg agataaggac ggtgcagtga tgtgccgtga 10621 ccaggtcccg caatcgttcc gacgggtcga tgttccggac ctccagtaca atatctgtat 10681 cgtctttccg gtacagggtc aggtaggccg ggtcggtgga cgagcaggat atccggaaga 10741 gaaactcgtt cagggtctcg gtagcgctcg tcgcatactc cccggccatc atgttcttct 10801 ccctgatgtg ctcgctgatc tgcataaggt cttcgacaac gccggacata tctgcgtaga 10861 gcgatccccg taccaccatc cggtcaaaga tggccgggtc aaaccagtct tcggtctcgc 10921 acgagttccg gagcccctgc atgaacgacc cgatattttg gatgatctgg acgattgccg 10981 cggcactctt gaggtgacgg cgaatgccgg acagctcccg ttctgcctgg acgagcaccg 11041 cttcaccgat ccggacgtcc atgatcccgg tcatcacctc cccgcagttg tgcgcctcgt 11101 cgatcagcag catggcgtcc cccggctcga ccccgaggga accgtagagc tggtcgcgga 11161 tatcctcgtt gaagaggtgg tggtagttga caatcacgac gtcagagcgc tgggcggcga 11221 gcaggagcat ctcgtagggg caggtctctg ccccgatatc cctgagctcg tcagcccgtg 11281 ccagttcccg ggagacccgc tcggcctttg ccttcagtgc ctgggacggg atcatccgga 11341 gacctgagcc ttcggcggac acaaagaccc tgctcctgat gtagtagggg cagagcaccg 11401 ggtgttcggg atccagtttc cggatctgct gcgtgatcag ggggtccttt gccgggacga 11461 gcgatccctg ctcagcccgt tcgcgcatca ggttcgtaga gatcgccttg accccttcac 11521 atcggcggta cacatccccc gcacccggga gaaggcagag gctccgtttc ccgatcaggt 11581 aggagtagga gatctggcgt ttcctcttcc ggatcagttc aagttccctg atgaacgtgt 11641 tcagctggct gatggtccgg accgcaacaa agatcctcct ccctgaccgt tcggagagga 11701 gtgcggcgat cacgctcgat ttcccgctcc cggtgggtgc atcgatcatc gcgattcccc 11761 cctcacgtgc gcactgcgcc acccgtgcaa gcatctcccg ctggtggggg cggtacgatg 11821 catacgggaa atactcgtcc agcttctcca tcatctagta attggtccgg actgtttttt 11881 attcttccag aggagagatc actctacatg gcaatccatc ccattgactt ccgctatggc 11941 accccggaga tgcgggcagt cttctccgag gagaaccggt ttctctccat cgtgcaggcc 12001 gagatcgcgc tcgcgagggc cggtgcggat tttgacctca tcccgccggt tgccgccagc 12061 cagataaaag agaatgccct ggcagcctct cttgcccgcg cgaaagagat agaggaagag 12121 atcaaccatg acatgatggc gatcgtgcga gcaatttcag aggtgtgcgg tgatgcaggg 12181 ggctatgtcc actacggcgc gacctcaaac gatatcctcg atacggcaac cggtctccag 12241 ctgaaagaag cccttgggat cctggatgcg aagctgcatt gcctcctcgc cgtcctggta 12301 tgccggagcc gcgagacacg gcacctggtc tgtgcagccc gga //