LOCUS DR991999 652 bp mRNA linear EST 04-AUG-2005
DEFINITION Mdlr7009P02.g1 Apple_EST_Mdlr Malus domestica cDNA similar to
pir|T00044 vacuolar sorting receptor protein homolog PV72 -
cucurbit dbj|BAA25079.1| PV72 [Cucurbita cv. Kurokawa Amakuri],
mRNA sequence.
ACCESSION DR991999
VERSION DR991999.1
DBLINK BioSample: SAMN00176548
KEYWORDS EST.
SOURCE Malus domestica (apple)
ORGANISM Malus domestica
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
Pentapetalae; rosids; fabids; Rosales; Rosaceae; Amygdaloideae;
Maleae; Malus.
REFERENCE 1 (bases 1 to 652)
AUTHORS Korban,S., Vodkin,L., Liu,L., Gasic,K., Gonzales,O., Hernandez,A.,
Aldwinckle,H., Malnoy,M., Carroll,N., Goldsbrough,P., Orvis,K.,
Clifton,S., Pape,D., Marra,M., Hillier,L., Martin,J., Wylie,T.,
Dante,M., Theising,B., Bowers,Y., Gibbons,M., Ritter,E., Ronko,I.,
Tsagareishvili,R., Kennedy,S., Waterston,R. and Wilson,R.
TITLE Apple Functional Genomics grant - NSF 0321702
JOURNAL Unpublished
COMMENT Contact: Schuyler S. Korban
Apple Functional Genomics grant - NSF 0321702
Washington University School of Medicine
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
Tel: 314 286 1800
Fax: 314 286 1810
Email: submissions@watson.wustl.edu
Library material provided by S. Korban/H. Aldwinckle/ M. Malnoy
Library constructed by D.O.Gonzalez/L.Vodkin Library sequenced by
Washington University Genome Sequencing Center
This trace has been recalled with phred
original value before phred recall for SL was 114
original value before phred recall for SR was 886.
FEATURES Location/Qualifiers
source 1..652
/organism="Malus domestica"
/mol_type="mRNA"
/cultivar="GoldRush"
/db_xref="taxon:3750"
/tissue_type="Leaves challenged with oblique banded leaf
roller (Choristoneura rosaceana)"
/clone_lib="SAMN00176548 Apple_EST_Mdlr"
/lab_host="DH10B ampicillin resistant"
/note="Vector: pBluescript II SK (+); Site_1: XhoI;
Site_2: EcoRI; Total RNA was extracted from freeze dried
leaf tissue, using the method described by Wang and Vodkin
(Plant Molecular Biology Reporter 12:132-145, 1994).
Poly(A)+ mRNA was isolated from total RNA using the
PolyATtract mRNA Isolation system III (Promega). The
library was prepared using the Stratagene pBluescript II
XR cDNA library construction kit. Complementary DNA was
synthesized from mRNA using a Poly (dT) sequence with a
XhoI restriction site. EcoRI adapters were ligated to the
blunt ended cDNA fragments followed by restriction with
XhoI. The cDNA insert is protected from XhoI digestion via
methylation during first strand cDNA synthesis. The cDNA
fragments were directionally cloned into the EcoRI-XhoI
restriction site of the pBluescript vector. The ligated
cDNA fragments were transformed into E. coli ElectroMax
DH10B host cells. Transformation efficiency: 4.0E+04
Average insert Size by PCR: 1320 bp"
BASE COUNT 176 a 113 c 195 g 168 t
ORIGIN
1 cacgagggcc atctgtgctg gtttccaaga gaccactgag ccctctgttt gtttaagtga
61 agatgtacaa acaaatgagt gtttagagaa caatggaggt tgctggcatg acaaagctgc
121 taacattacc gcatgcaggg atacattccg cggaagagtg tgcgaatgcc ctattgtgca
181 aggcgtaaag tttgttggtg atggttatac tcactgtgaa gcttcaggag cattacgttg
241 tggaataaac aatggaggtt gttggaagga aacccgatct ggcaggactt actctgcatg
301 tcgtgatgac catacaaatg gttgcagttg tccaccagga ttcaagggtg atggtgagaa
361 aacatgtgaa gatgttgatg agtgcaagga gaaaacggct tgccaatgtt cacaatgcaa
421 atgcaagaat acttggggaa gttacgagtg cagttgcggt ggcggtttac tgtacatgcg
481 agaaaatgat gcatgtatag gtaaaaacgg aagtggttct gggtctagct ggggcctaat
541 ttgggttatc attctctgct tgggtgctgt cggagttggg ggcttcgcag tttacaagta
601 ccgaatccgg agatacatgg attcggagat ccgggcaatc atggcgcagt ac
//