LOCUS       DQ173300                2186 bp    DNA     linear   BCT 23-AUG-2006
DEFINITION  Escherichia coli InsB (insB) and InsA (insA) genes, complete cds;
            insertion sequence ISEcp, complete sequence; and beta-lactamase
            CMY-7 (blacmy-7) gene, complete cds.
ACCESSION   DQ173300
VERSION     DQ173300.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Hopkins,K.L., Batchelor,M.J., Liebana,E., Deheer-Graham,A.P. and
            Threlfall,E.J.
  TITLE     Characterisation of CTX-M and AmpC genes in human isolates of
            Escherichia coli identified between 1995 and 2003 in England and
            Wales
  JOURNAL   Int. J. Antimicrob. Agents 28 (3), 180-192 (2006)
   PUBMED   16879949
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Hopkins,K.L. and Threlfall,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-AUG-2005) Laboratory of Enteric Pathogens, Health
            Protection Agency Centre for Infections, 61 Colindale Avenue,
            London NW9 5HT, United Kingdom
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E154371"
                     /db_xref="taxon:562"
     gene            complement(28..531)
                     /gene="insB"
     CDS             complement(28..531)
                     /gene="insB"
                     /note="IS1 transposase component; similar to Escherichia
                     coli InsB in GenBank Accession Number AY009096"
                     /codon_start=1
                     /transl_table=11
                     /product="InsB"
                     /protein_id="AAZ99134.1"
                     /translation="MPGNCPHYGRWPQHDFTSLKKLRPQSVTSRIQPGSDVIVCAEMD
                     EQWGYVGAKSRQRWLFYAYDSLRKTVVAHVFGERTLATLERLLGLLSAFEVVVWMTDG
                     WPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHY
                     LNIKHYQ"
     gene            complement(450..725)
                     /gene="insA"
     CDS             complement(450..725)
                     /gene="insA"
                     /note="IS1 transposase component"
                     /codon_start=1
                     /transl_table=11
                     /product="InsA"
                     /protein_id="AAZ99135.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHLKNSGRSR"
     regulatory      821..826
                     /regulatory_class="minus_35_signal"
     regulatory      845..850
                     /regulatory_class="minus_10_signal"
     mobile_element  901..924
                     /mobile_element_type="insertion sequence:ISEcp"
     gene            1041..2186
                     /gene="blacmy-7"
     CDS             1041..2186
                     /gene="blacmy-7"
                     /note="class C beta-lactamase"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase CMY-7"
                     /protein_id="AAZ99136.1"
                     /translation="MMKKSLCCALLLTASFSTFAAAKTEQQIADIVNRTITPLMQEQA
                     IPGMAVAVIYQGKPYYFTWGKADIANNHPVTQQTLFELGSVSKTFNGVLGGDAIARGE
                     IKLSDPVTKYWPELTGKQWQGIRLLHLATYTAGGLPLQIPDDVRDKAALLHFYQNWQP
                     QWTPGAKRLYANSSIGLFGELAVKPSGMSYEEAMTRRVLQPLKLAHTWITVPQNEQKD
                     YAWGYREGKPVHVSPGQLDAEAYGVKSSVIDMARWVQANMDASHVQEKTLQQGIALAQ
                     SRYWRIGDMYQGLGWEMLNWPLKADSIINGSDSKVALAALPAVEVNPPAPAVKASWVH
                     KTGSTGGFGSYVAFVPEKNLGIVMLANKSYPNPVRVEAAWRILEKLQ"
BASE COUNT          564 a          570 c          553 g          499 t
ORIGIN      
        1 tatcattgca gcggtaatga ctctaactta ttgatagtgt tttatgttca gataatgccc
       61 gatgactttg tcatgcagct ccaccgattt tgagaacgac agcgacttcc gtcccagccg
      121 tgccaggtgc tgcctcagat tcaggttatg ccgctcaatt cgctgcgtat atcgcttgct
      181 gattacgtgc agctttccct tcaggcggga ttcatacagc ggccagccat ccgtcatcca
      241 taccacgacc tcaaaggccg acagcaggcc cagaagacgc tccagcgtgg ccaacgtgcg
      301 ttcaccgaat acgtgcgcaa caaccgtctt ccggagactg tcatacgcgt aaaacagcca
      361 gcgctggcgc gatttagccc cgacatagcc ccactgttcg tccatttccg cgcagacgat
      421 gacgtcactg cccggctgta tgcgcgaggt taccgactgc ggcctgagtt ttttaagtga
      481 cgtaaaatcg tgttgaggcc aacgcccata atgcgggcag ttgcccggca tccaacgcca
      541 ttcatggcca tatcaatgat tttctggtgc gtaccgggtt gagaagcggt gtaagtgaac
      601 tgcagttgcc atgttttacg gcagtgagag cagagatagc gctgatgtcc ggcggtgctt
      661 ttgccgttac gcaccacccc gtcagtagct gaacaggagg gacagctgat agaaacagaa
      721 gccactggag cacctcaaaa acaccatcat acactaaatc agtaagttgg caccattacc
      781 cattgcagca aagatgaaat caatgattta tcaaaaatga ttgaaaggtg gttgtaaata
      841 atgttacaat gtgtgagaag cagtctaaat tcttcgtgaa atagtgattt ttgaagctaa
      901 taaaaaacac acgtggaatt taggaaaaac ttatatctgc tgctaaattt aaccgtttgt
      961 caacacggtg caaatcaaac acactgattg cgtctgacgg gcccggacac ctttttgctt
     1021 ttaattacgg aactgatttc atgatgaaaa aatcgttatg ctgcgctctg ctgctgacag
     1081 cctctttctc cacatttgct gccgcaaaaa cagaacaaca gattgccgat atcgttaatc
     1141 gcaccatcac cccgttgatg caggagcagg ctattccggg tatggccgtt gccgttatct
     1201 accagggaaa accctattat ttcacctggg gtaaagccga tatcgccaat aaccacccag
     1261 tcacgcagca aacgctgttt gagctaggat cggttagtaa gacgtttaac ggcgtgttgg
     1321 gcggcgatgc tatcgcccgc ggcgaaatta agctcagcga tccggtcacg aaatactggc
     1381 cagaactgac aggcaaacag tggcagggta tccgcctgct gcacttagcc acctatacgg
     1441 caggcggcct accgctgcag atccccgatg acgttaggga taaagccgca ttactgcatt
     1501 tttatcaaaa ctggcagccg caatggactc cgggcgctaa gcgactttac gctaactcca
     1561 gcattggtct gtttggcgag ctggcggtga aaccctcagg aatgagttac gaagaggcaa
     1621 tgaccagacg cgtcctgcaa ccattaaaac tggcgcatac ctggattacg gttccgcaga
     1681 acgaacaaaa agattatgcc tggggctatc gcgaagggaa gcccgtacac gtttctccgg
     1741 gacaacttga cgccgaagcc tatggcgtga aatccagcgt tattgatatg gcccgctggg
     1801 ttcaggccaa catggatgcc agccacgttc aggagaaaac gctccagcag ggcattgcgc
     1861 ttgcgcagtc tcgctactgg cgtattggcg atatgtacca gggattaggc tgggagatgc
     1921 tgaactggcc gctgaaagct gattcgatca tcaacggcag cgacagcaaa gtggcattgg
     1981 cagcgcttcc cgccgttgag gtaaacccgc ccgcccccgc agtgaaagcc tcatgggtgc
     2041 ataaaacggg ctccactggt ggatttggca gctacgtagc cttcgttcca gaaaaaaacc
     2101 ttggcatcgt gatgctggca aacaaaagct atcctaaccc tgtccgtgtc gaggcggcct
     2161 ggcgcattct tgaaaagctg caataa
//