LOCUS DQ083928 8137 bp DNA linear BCT 28-JUN-2005 DEFINITION Pseudomonas fluorescens strain 2P24 phl 2,4-DAPG gene cluster, complete sequence. ACCESSION DQ083928 VERSION DQ083928.1 KEYWORDS . SOURCE Pseudomonas fluorescens ORGANISM Pseudomonas fluorescens Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas. REFERENCE 1 (bases 1 to 8137) AUTHORS Wei,H.L., Wang,Y. and Zhang,L.Q. TITLE Identificaion and characterization of biocontrol bacterial strain 2P24 and CPF-10 JOURNAL Zhi Wu Bing Li Xue Bao 31, 80-85 (2004) REFERENCE 2 (bases 1 to 8137) AUTHORS Zhou,H.Y., Zhang,L.Q. and Tang,W.H. TITLE Clone, sequence analysis of phl 2,4-DAPG gene cluster from Pseudomonas fluorescens 2P24 JOURNAL Unpublished REFERENCE 3 (bases 1 to 8137) AUTHORS Zhou,H.Y., Zhang,L.Q. and Tang,W.H. TITLE Direct Submission JOURNAL Submitted (02-JUN-2005) China Agriculture University, College of Agronomy and Biotechnology, Yuanmingyuan West Road No 2, Beijing, Haidian District 100094, China FEATURES Location/Qualifiers source 1..8137 /organism="Pseudomonas fluorescens" /mol_type="genomic DNA" /strain="2P24" /db_xref="taxon:294" misc_feature 1..8137 /note="phl 2,4-DAPG gene cluster" gene complement(1..678) /gene="phlH" CDS complement(1..678) /gene="phlH" /note="similar to the IfeR of Agrobacterium tumefaciens encoded by sequence deposited in GenBank Accession number AF039653; similar to members of the TetR/AcrR family" /codon_start=1 /transl_table=11 /product="PhlH" /protein_id="AAY86545.1" /translation="MDNAIGKPSLPRASRLDGQATRLQILEKAGELFAEQGLANTTSK QICERSQANSAAVNYHFVNKEGLYRAVLLEAHARLVQLETLVSLNERPGSPQDKLRAL ITVLVERLHNHPDGWALKVLTREVLSPSPEFEVVLKEQSFPKAHILRGLLGQIMNLPA DHPTTLRSAISVFAPCLFLLIAHQPLKQHVLQGLSLEPQGLIDHMMSYALGGLQAVAA TAHDAAS" gene 787..1707 /gene="phlG" CDS 787..1707 /gene="phlG" /note="similar to the phlG of Pseudomonas fluorescens strain CHA0, encoded by sequence deposited in GenBank Accession Number AF0207529" /codon_start=1 /transl_table=11 /product="PhlG" /protein_id="AAY86546.1" /translation="MLRLLTHYQGNIVMEARVMTPFTYFSLPMQKQFLANQQAVQGKP YAEFFRSKISVPLSAVEKIQQGPMPLADTLTPSIEDLNRMLAADFISEEAGYALLPGP MAYVQSRKFFPNCTAEMLKWWFMWHPLEAERYTLWFPYAHVENPCVHHERLSDTTLSF EESLYGNTFCASEYVGDRLMHLHIHFRDPCELGFCPDLYRESKIDGSVSALMSLAHEP QVPVSLMAHLFKECPEGLYLTSRYWVGSHPAMQRFPGAERAAQLLEESGLGEVELETL AYEFAVHDMCEFNHLASILPSLHAQFSGAK" gene complement(1727..2353) /gene="phlF" CDS complement(1727..2353) /gene="phlF" /note="transcriptional repressor of 2,4-DAPG biosynthesis" /codon_start=1 /transl_table=11 /product="PhlF" /protein_id="AAY86547.1" /translation="MARKPSRSSIGSLRSPHTHKAIIISAIETLKECGYSGLSIEAVA RRAGASKPTIYRWWGNKAALIAEVYESESEQIRKEPDKGSFKENLNFLLLNLWKVWRE TICGEAFRCVIAEAQLDPSTLPKLKDEFMERRRELPRKLVENAIQQGELPKDTSRELL LDMIFGFCWYRLLTEQLEVEADINEFTTLLLNGVLRPTSAAVQGAAEA" gene 2771..3853 /gene="phlA" CDS 2771..3853 /gene="phlA" /note="similar to acyl carrier synthases; 2,4-diacetylphloroglucinoln (DAPG) biosynthesis" /codon_start=1 /transl_table=11 /product="PhlA" /protein_id="AAY86548.1" /translation="MNKVGIVSYGAGIPVCRLKVDDVIQVWKNTDLSLVKGQLGVIER AVLQPDEDVITLGVLAAQRALDEAPPCSLEALYLGTCTNPYDSRASAAIILEMLGCGY DAFCADVQFAGKSGTSALQIAYALVASGMVGNALAVGADTINRNTAPGDLTESYAGAG AAALLLGTENVIAHFDASFSCAADVADNIRPQGDRYIRSGMGLGPDKNSIGLEDQTRR AASGLMAKIHAQADDFDYVVFQQNLVSTPYSLGKHLGFTKAQIEPGIYAQSVGDAGAA SPLLGLVNVLDQARPGERILVVSYGFGAGSDAIALTVTDAIEAYQKTNVPLRTQLEDK YYVDYRTSIKYEFKYLRPDYALTAYL" gene 3884..5080 /gene="phlC" CDS 3884..5080 /gene="phlC" /note="similar to thiolase portion of SCO-x" /codon_start=1 /transl_table=11 /product="PhlC" /protein_id="AAY86549.1" /translation="MCARRVAIVSAAYTPKPGSSRVRQTFKEMIVESAYQALNAIKMH PRELQAVAYGYHGEGISEYGGLGPTISDALGISPAPTFMSTANCTSSSVSFQMAHQMV ASGEYDIVLCGGFEKMTDHFNYAEYIGSSTECEYDYFLGISHTDAFALATAEYFEKYG YAGREADVLATFGRQMRIYAHNTPTATRYGVPIPSLETLKSSEACGSMLAWGEASGCA ILVAEHLVHRYTTQPVFVRGCAYTGVSHYFGTRYHNPTLQFPGLPKDVGMAVSANSLA CAEIAYKKAGITAKDIDVAQVYDLLGAGLIQMESMGVCGPGQAGDFVLEGGIALDGQL PLNTDGGNIGRGHASGCDGILHITELFRQLRGESNNQVKGARIGVSQSLGGYAAHNSV IVLSND" gene 5091..5531 /gene="phlB" CDS 5091..5531 /gene="phlB" /codon_start=1 /transl_table=11 /product="PhlB" /protein_id="AAY86550.1" /translation="MSLYPEQIHRMTAASMLREWREHGGKYRLEGSRCQDCEEIFFPR RTVCGACNSLNVEPYRCARTGTIQVSAHAENPILAAMGYGETVPRFMAMVRLDDGLVI ASEIVDVIDPRQVVSGAPVRMVIRKHVRESNLAWQYAYKFVLVK" gene 5650..6738 /gene="phlD" CDS 5650..6738 /gene="phlD" /note="similar to chalcone synthase and stilbene synthases; putative synthase" /codon_start=1 /transl_table=11 /product="PhlD" /protein_id="AAY86551.1" /translation="MFLTLLAFSRGLVMSTLCKPSLLFPQYKITQQQMIEHLEQLHDD HPRMALAKRMIQNTQVNERYLVLPIDELAVHTGFTHRSIAYEREARRMSSIAARQAIE NAGLTTDDIRMVAVTSCTGFMMPSLTAHLINDLGLRTSTVQLPIAQLGCVAGAAAINR ANDFASLAPDNHVLIVSLEFSSLCYQPQDTKLHAFISAALFGDAVSACVMRADDQASG FKIAKTGSYFLPDSEHYIKYDVKDSGFHFTLDKAVMNSIKDVAPMMEELNYETFNQHC AQNDFFIFHTGGREILDELVLQLDLEPGRVAQSRDSLSEAGNIASVVVFDVLKRQFDS GPVNGATGMLAAFGPGFTAEMAVGKWVA" gene 6866..8137 /gene="phlE" CDS 6866..8137 /gene="phlE" /note="putative integral membrane protein" /codon_start=1 /transl_table=11 /product="PhlE" /protein_id="AAY86552.1" /translation="MEMKYLVARPWGGYERRMITLLSLSFGLVGLDRFIIMPLFPVIM HDLALDYQDLSMLSAILAFAWGGSALFMGAAIRVLGTKKLLVLSITLLSLLAGASALI TSLVGLVLLRALMGVCEGAFTPVSIIVTDEVSQPCRRGLNLGIQQALFPIIGLCLGPL LAGVLLEVFGSWRAVFAIISLPGLLVAWYLYKTYQPTQAPSTASIARTSRSQWRTALS SGNVRLNIALMLCILTCQFVLCALLPSYLTDILHLSNFSMSMIISAIGLGGFFGQLVI PGLSDQLGRKPVVSICFMVSALLVGLLIISPALPWLLFLQLFFLSFINFSLICITVGP LTSESVPPSLLATATGLVVGCGEILGGGVAPVVAGYIAMTWGLTAILFLALAGSLTGG LLSLWLKEANPVFNGLKDYAPLSARLTLEDK" BASE COUNT 1755 a 2345 c 2171 g 1866 t ORIGIN 1 tcagcttgca gcatcgtgcg cagttgccgc cactgcctgg aggccgccaa gggcatagct 61 catcatgtgg tctatcaagc cctgcggctc gagactcaac ccctgcagta catgctgctt 121 tagcggctga tgggctataa gcaggaaaag acagggtgcg aagacgctga tggcgctgcg 181 caacgttgtc gggtgatccg ccggcaagtt catgatttgt ccaagcaagc cacgcaggat 241 gtgtgccttg ggaaacgatt gctccttgag caccacctca aactcgggag aaggcgaaag 301 cacttcgcgc gtgagcactt tcagcgccca accatcggga tgattgtgca gtcgctcgac 361 cagcacggta atgagcgcac gcaacttgtc ctgcggcgaa cctggccgct cgttaagcga 421 aaccagcgtt tccagttgca ccaaccgagc atgggcttcg agcaacactg cgcgatacag 481 gccctcttta ttcacgaagt gatagttcac cgccgcactg ttggcttgcg aacgttcaca 541 aatctgcttg ctggtggtgt tggccaaccc ctgctcggca aacaattcac ccgccttttc 601 cagaatctgc agccgcgttg cctgcccatc gagccgcgaa gcacgaggca gggaaggctt 661 gccaatggca ttgtccatga gctcatctct ttatagttct aattcaaatt tgacttttta 721 tttcagccta cctattttgg tcccgtcatt gtccctttac aacaccgcgt gaggacttcg 781 cagccgatgc tgcggcttct cacgcactat caaggaaata tcgtcatgga agcgcgcgta 841 atgacacctt tcacctactt ctcattgcca atgcagaagc agttcctggc caatcaacag 901 gccgttcaag gcaaacccta cgccgaattt ttccgctcga agatcagcgt gccgctcagt 961 gctgtcgaaa aaatacagca aggccccatg ccgctggctg acaccttgac gcccagtatt 1021 gaagacctca accgcatgct cgctgctgat ttcattagcg aagaggcagg ctacgccttg 1081 ctgccagggc cgatggctta tgtgcaaagc cgcaagtttt ttcccaactg cacagctgag 1141 atgctcaagt ggtggttcat gtggcatcca ttggaggctg aacgctacac cttgtggttt 1201 ccttatgccc atgtggaaaa cccctgcgtt catcacgagc gtctcagcga cactaccttg 1261 agctttgagg aaagcttgta tgggaatacc ttttgcgcct ctgagtacgt cggcgaccgt 1321 ctcatgcacc tacacattca tttcagggat ccctgcgagc tgggcttctg tccggatctg 1381 tatcgcgaat caaaaataga cggcagcgtc agcgccttga tgagcctggc ccacgaaccg 1441 caagtgccgg tgtcgctgat ggcgcacttg ttcaaggaat gcccggaagg tttgtacctg 1501 acgagccgtt actgggtcgg cagccaccct gccatgcagc gtttcccagg cgccgagcga 1561 gccgcccagc tcctggaaga aagcggcctg ggggaagtgg agttggaaac cctggcttat 1621 gaatttgcag tgcatgacat gtgcgagttc aatcacctgg ccagcatcct gccgagcctt 1681 catgcgcaat tcagtggcgc aaagtaagga caaccccacc cgataatcag gcttcggcgg 1741 cgccctgaac cgctgccgaa gtcgggcgca ataccccgtt cagcagaagt gtcgtgaact 1801 cattgatatc cgcttccacc tccaactgct cagtcaacag ccgataccag cagaacccaa 1861 agatcatgtc caaaagcagt tcacgcgacg tatcctttgg caactcgcct tgctgaatgg 1921 cattttccac cagcttgcga ggcaactccc tacgccgctc catgaattca tctttcagtt 1981 ttggcagcgt actgggatcg agttgggcct cagcgatgac gcaccgaaaa gcctccccgc 2041 aaatcgtttc tcgccagacc ttccatagat tgagcagcaa gaaattgagg ttctccttga 2101 aggagccttt atcaggctct ttgcgaatct gctcgctttc actctcgtag acctcggcga 2161 tcaaggctgc cttgttaccc caccaccgat agatggtcgg tttactcgcg ccggcacggc 2221 gagccaccgc ctcgatgctc aaccccgaat aaccgcactc cttaagggtt tctatggcgg 2281 aaataatgat cgctttatgc gtgtgagggc tcctcaatga gccaatggag ctccgagatg 2341 gtttacgggc cataattcaa aatctccaat atggcgcttg acaaaagaga ctcagggtat 2401 aggattctct cgaaaagcaa tgaaacggat cgtttcgttt tgtatgttta tttgatctca 2461 gcttctgctt agcagcgggc ctctggagcg cttattgcct ctatgtaggg aaatcggaga 2521 aggaaagtca taaaatttta acgcaacctt aacgatacgg cacgtttcgt atcatacata 2581 acaattgtac aagaataaac ccattgttca catgctgacc ggtaggttcc gccttgtgaa 2641 cctgcctatt tacgggcata ggagcgccag tatcactttg cgggcaccaa tagatagttt 2701 ctaaatattt catgcgcttg tcacggcccc cggccaacag gtgaaagaac ggaatctgga 2761 ggatgtacac atgaataagg taggaattgt gagctatggc gcgggtatcc cggtctgccg 2821 gctcaaggtg gacgacgtga tccaggtgtg gaaaaacacc gatctgagtt tggtaaaggg 2881 ccaattgggc gttattgaaa gggccgtcct gcaacccgac gaagatgtca taaccttggg 2941 tgtcctcgcc gcccaacgcg cgcttgacga ggcaccacct tgttccctcg aagcgcttta 3001 tctcggcacc tgcaccaatc cttatgactc ccgggcctcg gccgcaatca tcctggaaat 3061 gctcggctgc ggctatgacg ccttttgcgc agacgtgcag tttgccggca aatcaggcac 3121 cagcgccctc cagatcgcat acgccttggt agcgtcgggc atggtgggca acgctttggc 3181 ggtgggtgcc gatacgatca accgcaacac cgcccccggt gacttgacgg agtcctatgc 3241 cggagccggg gccgcggcct tgctgttggg gacagaaaat gtgatcgcgc attttgacgc 3301 aagtttttct tgcgcggcgg atgtcgctga caacatcagg cctcaagggg accgctatat 3361 ccgctcggga atgggattgg gcccggacaa gaacagtatc ggcctcgagg accagactcg 3421 ccgcgccgcc tcgggattga tggccaagat ccatgcgcaa gccgatgatt tcgattacgt 3481 cgtcttccaa caaaacctgg tgtcgacccc ctactctctc ggcaagcacc tgggattcac 3541 gaaggcccaa atcgaacctg gcatctacgc ccagagtgtc ggcgatgctg gagccgcgag 3601 tcctttactg ggcttggtca acgtactcga tcaagcgcgt cctggcgaaa gaatcctggt 3661 ggtttcctat ggcttcggtg cgggcagcga tgccatcgcc ttgaccgtca ccgatgccat 3721 cgaagcctat caaaagacca acgttccgct gcgcacgcag ctggaggata agtactacgt 3781 ggattacagg acgtcgatca agtacgagtt caaatacttg cggcccgact atgccctgac 3841 ggcctatctc tgattccaat cattcagcaa ggagcacctt tttatgtgcg cacgacgcgt 3901 tgctatcgtt tcggctgcct atacccctaa acctggcagt tccagagttc gccagacttt 3961 caaggagatg atcgtcgagt ccgcctacca ggcactcaac gccatcaaga tgcaccctcg 4021 cgaacttcag gcagttgcct atgggtacca tggtgaaggt atttctgaat acggaggtct 4081 gggcccaacc atctccgatg ccttggggat cagccctgcc cccacgttca tgagtacggc 4141 caactgcacc agcagttcgg tgtctttcca gatggctcac caaatggtcg cctcaggtga 4201 atacgacatc gttctgtgtg gcggctttga aaaaatgacc gatcacttca actatgccga 4261 gtacattggc tcgagcactg aatgtgaata cgattatttt ctaggcatct ctcacacaga 4321 cgccttcgcc ctggcgaccg ccgaatattt tgaaaagtac ggctatgcag gacgcgaagc 4381 ggatgtattg gccactttcg ggcggcagat gcgcatttat gcccataaca ctcccacggc 4441 tacccgttat ggcgtgccga tcccgtctct cgaaacactc aaaagcagcg aagcatgcgg 4501 ctccatgctg gcctggggtg aagccagcgg ttgcgccatc ctggttgccg aacaccttgt 4561 gcaccgctac acgacccaac cggtatttgt ccgtggctgt gcctacaccg gcgtgtcgca 4621 ttatttcggc acgcgctatc acaacccgac gttgcaattt ccggggctgc caaaagatgt 4681 cggcatggcg gtttccgcca actcgctggc ctgcgccgaa atcgcctaca aaaaagccgg 4741 tatcaccgca aaggatatcg atgtggcgca agtctacgac ttgctgggcg ccgggttgat 4801 tcaaatggag tccatgggcg tatgtggtcc aggccaggca ggcgactttg tattggaagg 4861 cggcatagcc cttgatgggc aacttcccct gaacactgac ggcggcaaca tcggacgcgg 4921 ccacgcttcc ggttgcgatg ggattttgca cattactgaa ttgttccggc agcttcgcgg 4981 cgagtccaac aatcaagtca aaggcgcgcg tattggcgtc tcgcaaagcc tgggcggtta 5041 cgccgcgcac aacagcgtca ttgtgctttc caacgattaa ggagccgacc atgtcccttt 5101 accctgaaca aattcaccgc atgaccgccg ccagcatgtt gcgcgaatgg cgcgaacacg 5161 gtggcaaata ccgcctcgaa ggcagccgat gccaggactg cgaagaaatc ttcttccctc 5221 ggcgcacagt ttgcggtgcg tgcaactcgt tgaatgtaga accgtatcgc tgcgcgcgca 5281 ccggcactat ccaggtcagc gcacacgccg agaaccccat tcttgcggcc atgggctacg 5341 gcgaaaccgt gccccgcttc atggcgatgg tgcgcctgga tgatggtctg gtgattgcct 5401 cggaaatagt tgacgtcatc gatccgcgac aagtcgtcag cggtgcgccc gtgcgcatgg 5461 tcattcgaaa acatgtccgg gaaagtaact tggcctggca atatgcctat aagtttgtat 5521 tggtgaaata acaaacttag acgccgccat accccgcgtt atcgataaca acagtcaagt 5581 ttttggcctc caagcccgtt gctttgattc tgtctttcag agtccagctt tgctgttatt 5641 gccttaaaca tgtttctaac tttattggct tttagccgag gacttgtcat gtctactctt 5701 tgcaaaccca gtctgctgtt cccgcaatat aagatcaccc agcaacaaat gatcgagcat 5761 ctagagcagt tgcatgatga tcacccgaga atggctcttg ccaaacgcat gattcaaaac 5821 acacaggtga acgaacgata cttggtcctg cccattgatg aactggcggt gcataccggc 5881 ttcacccatc gcagcattgc ttatgagcgc gaggcccgcc gcatgtcatc catcgcggcg 5941 cgccaagcca tcgagaatgc cgggctgacc accgacgaca ttcgaatggt cgctgtgaca 6001 tcgtgcacgg gtttcatgat gccttcgctg acggcccact tgatcaacga cctgggcctg 6061 cgaacatcga ccgtacaact gcctatcgct caattgggat gcgtggcagg cgctgcggcg 6121 atcaatcgag ccaatgactt cgccagcctg gcgccggaca accatgtgct catcgtctcc 6181 ctggagttct cgtcgctctg ctatcaaccc caggacacca agttgcacgc attcatctca 6241 gcggcattat tcggcgatgc ggtatcagcc tgcgtaatgc gagccgacga tcaggcgtct 6301 ggtttcaaga tcgccaagac ggggtcttac ttcctgcctg atagcgagca ctatattaaa 6361 tacgacgtca aagacagtgg ctttcacttc accctggaca aggcggtcat gaactccatt 6421 aaagatgtcg cacccatgat ggaggaattg aactacgaga ccttcaatca acattgcgct 6481 caaaatgact ttttcatctt ccatacaggc ggacgggaaa ttcttgatga actggtcctg 6541 caattggact tggaacccgg cagggtcgcg caatcccgcg acagtttgag cgaagccggg 6601 aacatcgcca gcgtggtggt cttcgatgtg ctcaagcgcc agttcgacag cggacctgtc 6661 aatggcgcga cgggcatgtt ggcggccttc ggcccgggtt tcaccgctga aatggccgtg 6721 ggcaagtggg tcgcctgaca gaccggataa aacaccagca cccagccagg ggcacacctg 6781 cccctggcta cgggcgtatt gatcgcaggc gttcaacctc gttcgagaga gcttcgaaag 6841 cgctagggaa agaaggggaa agaacatgga gatgaagtat ctggtcgctc gaccttgggg 6901 cggctatgaa agacgcatga tcacgctttt gtcgctgagc ttcgggctgg taggactgga 6961 caggttcatc atcatgccgc tgttcccggt gatcatgcat gacttggccc tggactatca 7021 ggacttgagc atgctgtcgg ccatcctggc atttgcatgg ggaggctcgg ccctgttcat 7081 gggtgccgcc atcagggtat tgggcaccaa gaagttgctg gtgctgtcga tcaccctgct 7141 atccctcctg gccggtgcgt ccgcattgat caccagcctg gtgggcctgg tgctcttgcg 7201 tgccttgatg ggcgtctgtg aaggtgcatt caccccggtc agtatcattg ttaccgatga 7261 agtctcgcag ccctgccgac gcggactcaa tctgggtatt caacaggcat tgttccccat 7321 catcggcctg tgcctcggac cgctgctggc gggcgtcttg ctggaagtgt tcggatcctg 7381 gcgtgccgtg ttcgccatca tttcgttacc cggcctgtta gtggcctggt atctctataa 7441 aacttaccaa cccacccagg cgcccagcac ggcgtcgatt gctcgcacct ccagaagcca 7501 gtggaggaca gccctgtcca gcggcaatgt gcggctcaat attgccttga tgctgtgcat 7561 cctgacgtgc cagttcgtgc tgtgtgccct gctccccagc tacctgaccg acattctgca 7621 cctgagcaat ttctccatgt ccatgatcat ttccgccatt ggcctgggtg gtttctttgg 7681 ccaactggtc attcccggct tgtccgacca actgggtcgc aagcccgtgg tttccatctg 7741 cttcatggtc agcgcattgt tagtgggctt attgatcatc agccctgcgc tgccctggct 7801 actgtttctt caattatttt tcttgtcctt catcaacttc agcctgatct gcataacggt 7861 tggcccgctg accagcgaat cagtaccgcc cagtttattg gccaccgcga cagggctggt 7921 ggtgggctgc ggagagatcc tgggcggagg cgtcgcgcct gtcgtcgccg gctacatcgc 7981 catgacgtgg ggcctcaccg caatcctgtt cctggcactg gccggaagtt tgaccggtgg 8041 gttgctgtcc ttgtggctca aagaggccaa cccggtcttc aacgggctca aggactatgc 8101 ccccctatcc gcacgcttga ccctggagga taagtaa //