LOCUS DNQV01000089 11405 bp DNA linear ENV 07-SEP-2018 DEFINITION TPA_asm: Clostridiaceae bacterium isolate UBA10997 contig_1304, whole genome shotgun sequence. ACCESSION DNQV01000089 DNQV01000000 VERSION DNQV01000089.1 DBLINK BioProject: PRJNA417962 BioSample: SAMN08018685 Sequence Read Archive: SRR6482779 KEYWORDS WGS; Third Party Data; TPA; TPA:assembly. SOURCE Clostridiaceae bacterium (fermentation metagenome) ORGANISM Clostridiaceae bacterium Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae. REFERENCE 1 (bases 1 to 11405) AUTHORS Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A., Chaumeil,P.A. and Hugenholtz,P. TITLE A standardized bacterial taxonomy based on genome phylogeny substantially revises the tree of life JOURNAL Nat. Biotechnol. (2018) In press PUBMED 30148503 REMARK Publication Status: Available-Online prior to print REFERENCE 2 (bases 1 to 11405) AUTHORS Parks,D.H. TITLE Direct Submission JOURNAL Submitted (04-APR-2018) School of Chemistry and Molecular Biosciences, University of Queensland, Chemistry Bld, Cooper Road, St Lucia, Brisbane, Queensland 4072, Australia COMMENT Annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (released 2013). Information about the Pipeline can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Assembly-Data-START## Assembly Method :: CLC de novo assembler v. 4.4.1 Expected Final Version :: yes Genome Coverage :: 312.11x Sequencing Technology :: Illumina HiSeq 2000 ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Date :: 04/27/2018 21:05:32 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline Annotation Method :: Best-placed reference protein set; GeneMarkS+ Annotation Software revision :: 4.5 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total) :: 2,237 CDS (total) :: 2,194 Genes (coding) :: 2,160 CDS (coding) :: 2,160 Genes (RNA) :: 43 tRNAs :: 40 ncRNAs :: 3 Pseudo Genes (total) :: 34 Pseudo Genes (ambiguous residues) :: 2 of 34 Pseudo Genes (frameshifted) :: 10 of 34 Pseudo Genes (incomplete) :: 19 of 34 Pseudo Genes (internal stop) :: 6 of 34 Pseudo Genes (multiple problems) :: 3 of 34 CRISPR Arrays :: 2 ##Genome-Annotation-Data-END## FEATURES Location/Qualifiers source 1..11405 /organism="Clostridiaceae bacterium" /mol_type="genomic DNA" /isolate="UBA10997" /isolation_source="fermentation" /db_xref="taxon:1898204" /environmental_sample /note="metagenomic; derived from metagenome: fermentation metagenome" gene complement(35..277) /locus_tag="DDW58_05060" CDS complement(35..277) /locus_tag="DDW58_05060" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_008907833.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="acid-soluble spore protein" /protein_id="HBG38605.1" /translation="MAAGQSGSGRTQLIPESHSKLDDMKYEIAGELGIPVYQGSEDYW GSIPSRDCGRVGGSMVKRMIAMAEEEIANGKMPNKI" gene 671..>1079 /locus_tag="DDW58_05065" CDS 671..>1079 /locus_tag="DDW58_05065" /inference="COORDINATES: protein motif:HMM:PF01522.19" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="deacetylase" /protein_id="HBG38606.1" /translation="MKVIFVPKKVLNNIILVLIMVLISITYSLTDGYKYANVFLKSQR EIPIYSVDTNEKKISLTFDVAQDEGYIDEILNILDANNIRATFFIVGDWVDNYPGKVK EIYDKGHEIGNHSNSHPHFSKIQPEKMKQEILIL" assembly_gap 1080..1374 /estimated_length=295 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene <1375..1632 /locus_tag="DDW58_05070" CDS <1375..1632 /locus_tag="DDW58_05070" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_008907834.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="deacetylase" /protein_id="HBG38607.1" /translation="IQWDVDSIDWKDPGKEYIYNKMINNTGNGSILLFHNYAKDTPEV LDSIIKELKRKGFEFVKISDLIYKDNYYIDNIGRQKKILNN" gene 1723..2310 /locus_tag="DDW58_05075" CDS 1723..2310 /locus_tag="DDW58_05075" /inference="COORDINATES: ab initio prediction:GeneMarkS+" /note="Derived by automated computational analysis using gene prediction method: GeneMarkS+." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="HBG38608.1" /translation="MIKIGVIGSRGKTKVCSIIKDKLLKYNKKIIIANGQIENIGEVS HIDCDTDIVIYIFYKEQYQIIKDKVKLDILIQTAFDDEDHQYFNMLQDIISLIKKDGY IIFDSDFSEKISFYCENIYPITYGLNSKSTVTASSIDDTDKLQFSYCLQRGIYTISNE IIKPFEKPFIESGSSDEILYYLAALTCILIIDYKF" gene 2401..3084 /locus_tag="DDW58_05080" CDS 2401..3084 /locus_tag="DDW58_05080" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_012620885.1" /note="binds to single stranded DNA and may facilitate the binding and interaction of other proteins to DNA; Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="single-stranded DNA-binding protein" /protein_id="HBG38609.1" /translation="MIDGLMATNKVYAEGKVISELVFSHEMYGEGFYNFKIEIPRLSN TSDILPVTVSERLMVAVDLSIGKMISVEGQLRSYNKFVEGSNRLILTVFARDIKNLEE ISKSPNQIFIDGYICKPPIYRTTPFGREITDLLVAVNRPYNKSDYIPAIAWGRNAKFS SNLEVGTHIKLWGRIQSREYEKKVSEENIIKRVAYEVSISKMECCDKDEKESVEEIEG TEETDEIDE" gene complement(3205..3550) /locus_tag="DDW58_05085" /pseudo CDS complement(3205..3550) /locus_tag="DDW58_05085" /inference="COORDINATES: similar to AA sequence:RefSeq:YP_001255645.1" /note="frameshifted; incomplete; missing start; Derived by automated computational analysis using gene prediction method: Protein Homology." /pseudo /codon_start=1 /transl_table=11 /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase" assembly_gap 3551..3917 /estimated_length=367 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene complement(<3918..>4063) /locus_tag="DDW58_05090" CDS complement(<3918..>4063) /locus_tag="DDW58_05090" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_008907837.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=2 /transl_table=11 /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase" /protein_id="HBG38610.1" /translation="EIAKYIREAKKSTPVKAYIEGNLNGVYEAPIDLYGSDGFYVAFG ENTD" assembly_gap 4064..4277 /estimated_length=214 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene complement(4383..5549) /locus_tag="DDW58_05095" CDS complement(4383..5549) /locus_tag="DDW58_05095" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007505074.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="aminotransferase A" /protein_id="HBG38611.1" /translation="MEELINKRVKEIQISGIRKFYNMTLKYPDVISLTLGQPDFNTPE HVKEAGIKAINANKTKYTENPGLLELRKEISKYIKLKYGMDYDPYNEIIVTNGASEGI DSTLRTILDEGSEVILPGPVYPGYEPIIKMCGAVPKYIDTRKNNFKITAEDIGKNITS KTRCIILPYPSNPTGAVLTKDEVQKIADVLRDREIFVLSDEIYSELVYDRNHFSIASI PGMKEKTIVINGLSKSHSMTGWRIGFILGPSYLTKHIVKVHQYNATCASSISQYAALE AVKNGSDDPLAMKNEYKKRRDYIFNRLINMGFEVAKPDGAFYIFPSISKFNMNSFEFA SKLLEKQRVAVVPGDAFSSYGEGYIRLSFACSMDTLNEALNRIERFINIDSINL" gene complement(5665..6414) /locus_tag="DDW58_05100" CDS complement(5665..6414) /locus_tag="DDW58_05100" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_008907844.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="4-hydroxy-tetrahydrodipicolinate reductase" /protein_id="HBG38612.1" /translation="MNILLHGCNGRMGQVLTRLIPEEDDMNIAAGVDSDVNKFKNSYP VYNSLKQVKEKFDLLIDFSNHTAIENILNFGLSKKVPLVICTTGFTDEEKNKMLEASK QIPIFNSSNMSLGVNLLISLSKQAAQVLGSDYDIEIIEKHHNQKLDSPSGTALMIADA INKTLGNNSNYVYGRHSKTSKRDKNEIGIHSVRGGGVVGEHEVLFLGSGDVIEIKHSA ISRDVFGYGAIKAARFIIDKNPKLYSMDDLI" gene complement(6425..7306) /locus_tag="DDW58_05105" CDS complement(6425..7306) /locus_tag="DDW58_05105" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_008907845.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="4-hydroxy-tetrahydrodipicolinate synthase" /protein_id="HBG38613.1" /translation="MSVFTGSGVAIVTPFNKEGVDFEKLGELLEWHIKEKTDAIIICG TTGESSTMSLDEKKETIKYTVNKVNGRIPVIAGTGSNNTKAAVEMSVWAESIGVDALL LITPYYNKTTQRGCIEHFKAIANSVTKPIIIYNVPGRTGLNLLPQTLYELSKIKNIVA VKEASSNIDQISEIARLCGDNLDIYSGNDNETIPIMSLGGKGVISVLANILPRDTHDL CQKFLDGDLKGARDMQLKLLPLMNALFIETNPIPVKTAMNLMGMNVGHLRLPLVDMSE KNLEALKKEMINYGIKL" gene complement(7381..8364) /locus_tag="DDW58_05110" CDS complement(7381..8364) /locus_tag="DDW58_05110" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_012103547.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="aspartate-semialdehyde dehydrogenase" /protein_id="HBG38614.1" /translation="MGVKVAVVGATGLVGRKILEVLQEKNFPIDKLYLFASQKSAGKT MMFKDKEYTVEELKEDSFDRGIDIALFSAGASTSLKFAPIASQKGCIVVDNSSAWRMD KNVPLVVPEVNPEDISWNKGIIANPNCSTIQAVVALKPLNDKFNIRRIVYSTYQAVSG AGQVGYSDLVNGVKGEQPKKFPYPIAYNVLPHIDSFMENGYTKEEIKMVNETRKILHN DSLRITATTARVPVFYGHCESINIEFEKSFDMKDIFSTLNSAPGVIVYDDVKNQIYPM PITVEGKDEVYVGRIRRDESVDSGINIWVVADNIRKGAATNAVQIAQLLLK" gene 8609..8839 /locus_tag="DDW58_05115" CDS 8609..8839 /locus_tag="DDW58_05115" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_008907851.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="spore protein" /protein_id="HBG38615.1" /translation="MTTPFKKVVKAKIKAKKELSPVEKERELIKYEIAEELGLKDRVE KYGWSGLTAEETGRIGGIMTKMNKDAGRRWND" gene complement(8840..9793) /locus_tag="DDW58_05120" CDS complement(8840..9793) /locus_tag="DDW58_05120" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_006316463.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="mannose-6-phosphate isomerase" /protein_id="HBG38616.1" /translation="MEPLFFDPIYKSIIWGGRNLERIFNRSLPEGKVAESWEISNHGK DNSIITNGEFKGKSLNELLNKFGSKLVGKKCSSERFPLLIKLIDANDKLSVQVHPDNE YAKIHDNDLGKTEMWYIIDAKPNAKLICGVKSGTTKKQFEEAIKNNTLNEYLNYVDVK KGDTIFIPSGTVHAILDGIVIAEIQQNSDTTYRVYDWGRVGKDGKPRELHVEKALDVI NFEYKGSVEKVSTKKEEGFEHTSLINCKFFNVDKIHVFNSYKDKTDGSTFFAYTCVEG NGKLKYKDNYFDIKGGTSFLIPADNLEFEIAGNLQLLKSYI" gene complement(9941..10198) /locus_tag="DDW58_05125" CDS complement(9941..10198) /locus_tag="DDW58_05125" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_009242210.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="HPr family phosphocarrier protein" /protein_id="HBG38617.1" /translation="MQKFIYTITDPQGLHARPAGLLVKCAQKCTSSVQMAANGHSADA KSILAVMGLGVTKNNEITFSVEGASEAKDAAALKDFCEKNL" gene complement(10256..>11405) /locus_tag="DDW58_05130" CDS complement(10256..>11405) /locus_tag="DDW58_05130" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_004165378.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=2 /transl_table=11 /product="PTS fructose transporter subunit IIBC" /protein_id="HBG38618.1" /translation="PTRAHTYSSSKEVTKETEKEKKESSGDSAGKTAMKQLMNGVSYM IPFVVVGGLFIAISISIGGKPTAKGMVIPPGAWDKVNQIGGIGFNLMIPILAGYIAYA ISGRAALAPAMISAVVANSKEILGTSAGTGFLGAIFVGYLTGYLVKWMNSWKIPRSLK PIMPIFVIPLLGTAAVSAVLILFLGAPISWLMTALNSALTFLSKDPVTAIPLGLLLGA MVAFDMGGPVNKVAFLFGTASIVGGTPQIMGAVACAIPVPPLAMGLATLIDKKCFNEE ERAAGIPALLMGLIGITEGAIPYAACDPKHVMPSIIVGSSVASALGMVLGITDIVPHG GPIVGFLGATNSLPLFLLTIAAGTVISTLMVIALKGIKQKKEFASKVV" BASE COUNT 3344 a 1855 c 1696 g 3634 t ORIGIN 1 gggtgttaac attttatgtg ttaacaccct aaaattaaat tttatttggc atttttccat 61 tggcaatttc ttcttctgcc attgctatca tgcgtttaac cattgagcca ccaactctgc 121 cgcaatccct tgatggtata cttccccagt aatcttcaga accttgatat actggtatac 181 caagttctcc tgcaatttca tatttcatat catcaagttt agaatgagac tctggtatta 241 actgagttct acctgagcca ctttgtcctg ctgccatatt tattcctcct tatgtagttt 301 tatttactat taatttgtgc atagttttta atattatgct agtaatttaa tggaaacagg 361 attatttttc tctgttgtga gtttttttag ttatctttgg attaccttca ttttgggctt 421 tttgtgagcg gctttcccgc ccttttggat ggtcagtttt ttcaccatca gaaagagcaa 481 gctccatatt gtacttttta aaagtctcgg gatttaattt tttatgagac attttgtttc 541 ctccttaata aatagtaaaa tttaaatatt attaacctgt acatttttat acaaaactat 601 aaacaactta tgaataaaaa aatgaaaaaa tatatagata taaataaaga aataaataag 661 agggatatca ttgaaggtta tttttgttcc taaaaaagta ttaaataata ttatccttgt 721 tcttattatg gttcttatat ctattactta ttcactaaca gatggatata aatatgctaa 781 tgtttttttg aaatcacaaa gggaaatacc aatttattct gtggatacaa atgaaaaaaa 841 gatttcttta acttttgatg tagctcaaga tgaaggatat attgatgaaa tattgaatat 901 ccttgatgca aacaatataa gagccacttt ttttattgta ggtgactggg ttgataacta 961 tcctggaaaa gttaaggaga tatatgataa agggcatgaa attggaaacc actcaaattc 1021 acatcctcat ttttcaaaaa tacagcctga aaaaatgaaa caggaaatac tgatattaan 1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnattcaa 1381 tgggatgtag attcaattga ttggaaggat cctggtaagg aatatattta taataaaatg 1441 ataaataata caggtaatgg atctatttta ttattccata attatgctaa ggatactcca 1501 gaagttttag attcaataat aaaggagcta aaaaggaaag ggttcgagtt tgtaaaaata 1561 agtgatctta tttataagga taattattat attgataata taggaaggca aaaaaaaata 1621 cttaataatt aggctgttta ttaaagatat gccgtataat gaataattaa actattttgt 1681 gcaaattata agatgaaaaa gataaggttt tgaggtgaaa ctatgattaa aataggggta 1741 ataggaagca gagggaaaac gaaagtatgc agcattataa aggacaaatt attaaaatat 1801 aataagaaaa ttataatagc aaatggtcaa attgaaaata ttggtgaggt aagccatata 1861 gactgtgata cagatatagt aatttatatt ttttataagg agcaatatca aataattaag 1921 gataaagtca agcttgatat attaattcaa actgcatttg atgatgaaga tcatcaatat 1981 tttaatatgc ttcaagatat tatatcttta attaaaaaag acggctatat tatttttgat 2041 tcagatttta gcgaaaagat aagcttttat tgcgaaaata tataccctat aacctatgga 2101 ttaaatagta aatctacagt aactgcttca agtattgatg atactgataa gcttcaattt 2161 agctattgcc ttcaaagagg aatctataca atttccaatg aaataataaa gcccttcgaa 2221 aagcctttta tagaaagtgg aagttctgat gaaatattgt attaccttgc agcacttact 2281 tgtattttaa taatagatta taaattttaa aaaacaatca taaaattgat tgtagagtaa 2341 tatttataat ttagaattca ttattaataa ttattggaat acgaagggag agggtgggaa 2401 atgattgacg gattaatggc tactaataag gtttatgcag aaggtaaagt aatctcggag 2461 ttggtattta gtcatgagat gtatggggag ggtttttata attttaaaat tgaaattcca 2521 agattaagca atacttcaga tattttacca gttacagtat ctgaaagact catggttgca 2581 gtggatttgt ctattggcaa aatgataagt gttgagggac agttaagatc ttacaataaa 2641 tttgtagagg gcagcaatag attaatactg actgtatttg cacgggatat aaaaaactta 2701 gaagaaattt caaagagccc aaaccaaatt tttattgacg gctatatttg taaaccccca 2761 atttatagga ctacaccttt tggaagggaa attactgatc ttttagttgc agttaatagg 2821 ccctacaata agtctgatta catacccgct attgcatggg gtagaaatgc taaattttca 2881 agtaatcttg aagttgggac ccatataaag ctttggggga gaatacaaag cagggaatat 2941 gagaaaaaag tttcagaaga aaatattatt aaaagagttg cttatgaagt ttctatttca 3001 aagatggaat gttgtgataa ggatgaaaaa gaatcagttg aggagataga gggtacagag 3061 gaaacagatg agattgatga atgattttta aggagctgtt gtactaagaa tagatactac 3121 aatatattgt gctaacttta aatttgcaaa tcaatatatt gtatgtaaat tccttagtac 3181 aacagctcct catactgata taaattacct tcttaagtct tccattattt tagttttatt 3241 ttttgttttt aagtcaacaa tttttgtaac ttttgcagga gctccagcta ctacaactcc 3301 gctgtcaaca ttcttggtta ctatagcacc agctgccaca actgaacctt tgccaattct 3361 aacaccttca agaactcact gcatttgctc caattaaaac attatcctca attataacag 3421 ggttgctgct tggcggctct aatactcctg ctattactgc gcctgcgcct acgtgcacat 3481 ttttccctat cttacctctt gctccaacta ctgcattcat gtcaaccatt gaattttcac 3541 caatttctgc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3901 nnnnnnnnnn nnnnnnntat cagtattttc tccaaaagca acataaaatc catcacttcc 3961 atataaatca atgggagctt cataaactcc atttaaattt ccttctatat atgcttttac 4021 tggagtagat tttttagctt ctcttatata ttttgcaatt tcannnnnnn nnnnnnnnnn 4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4261 nnnnnnnnnn nnnnnnnatc atattatcac tcctacaata cctataatga ttaaacgaaa 4321 aatttagttt taattactaa taaactaagg accaaaaaaa aatctttttt ttaaatatat 4381 cattataaat ttatactgtc aatattgatg aacctttcta ttctgtttaa agcttcattg 4441 agggtatcca tagaacatgc aaaggacaat cttatatagc cttctccata ggatgaaaaa 4501 gcatctccgg ggacaactgc aaccctttgc ttttctaaaa gtttacttgc aaactcaaaa 4561 gagttcatat tgaattttga aatagatggg aatatataaa aagctccatc aggctttgca 4621 acttcaaagc ccatgtttat aagcctatta aaaatataat cccttctttt tttatattca 4681 tttttcatag ctaagggatc atcagagcca tttttcactg cttctaaagc ggcatactga 4741 cttatagatg aagcacaagt agcattatac tgatgtactt taacaatgtg tttggttaaa 4801 taagaaggac caagtataaa gccaattctc cagcctgtca ttgaatgaga ctttgaaagc 4861 ccatttatta caatggtttt ttctttcatt cctggaatcg atgcaataga aaagtgattt 4921 ctatcatata caagttcact gtatatttca tctgatagta caaaaatttc cctatccctt 4981 aatacatctg caattttttg aacctcatcc tttgttagta ctgctcctgt tgggtttgaa 5041 gggtagggga gtattataca cctagttttt gatgttatat tttttcctat atcctcagct 5101 gttattttaa aattgttttt tcttgtatca atatatttag gaacagcacc acacatttta 5161 attataggtt catatccagg ataaactgga cctggaagaa ttacttccga gccttcatcc 5221 aatatagttc ttagggtact gtcaatgcct tcactggcac cattagttac tattatttca 5281 ttataaggat cataatccat gccatacttt aattttatat acttggatat ttccttcctt 5341 aattcaagca aaccagggtt ttcagtatat tttgttttat ttgcattaat tgctttaatt 5401 cctgcctctt taacatgttc aggggtatta aaatcaggtt gacctaaggt taaggaaata 5461 acatcaggat attttaatgt catattgtaa aattttctta ttcccgaaat ttgaatttcc 5521 tttactcttt tgtttataag ttcctccata taaacaatcc tttcttttat ccaatggcta 5581 catccgtaat accctgccta aggcgaggta ttacggatac tacacccatg ggttcttcta 5641 agataaaaga cccacaatgg ggtcttaaat caaatcatcc atactatata atttagggtt 5701 cttatcaata ataaaccttg cagcttttat agcaccataa ccaaaaacat ctcttgaaat 5761 agcactatgc ttgatttcaa ttacatctcc tgatcctaaa aataatactt catgttctcc 5821 aacaactccg ccgcctctta cagaatgtat tccaatttca tttttatctc ttttcgaagt 5881 ttttgaatgt cttccataaa cataatttga attgtttcct aaagttttat ttatagcgtc 5941 agcaatcatg agggcagttc cgcttggaga atcaagcttt tgattgtgat gcttctcaat 6001 tatttctata tcataatcag agcctaaaac ttgtgcagcc tgttttgata gagaaattaa 6061 aagatttact ccaagagaca tatttgagga attaaaaatt ggaatttgtt ttgaagcctc 6121 taacatttta tttttttctt catctgtaaa accagtcgta caaatgacta aagggacttt 6181 tttacttaat ccgaaattta gtatattttc aattgctgta tgatttgaaa agtcaattaa 6241 taaatcaaat ttttccttaa cctgttttaa actattataa acaggatagg aattcttaaa 6301 tttatttaca tcgctgtcaa ctcctgcagc aatattcata tcatcctctt cagggatgag 6361 tcttgttaat acctgcccca ttctaccatt acatccatga agtaaaatat tcatactttc 6421 acccctaaag cttgattcca taattaatca tttccttttt aagagcctca aggtttttct 6481 cagacatatc cacaagcggg agtctaagat gtccaacatt cattcccata aggttcatgg 6541 cagttttaac tggtattgga ttggtttcaa tgaacagggc attcataagc ggtaaaagtt 6601 taagctgcat atcacgtgct ccttttaagt ccccatctaa aaacttttgg cacaagtcgt 6661 gggtatccct tggcaatata tttgcaagta ctgaaataac accttttccg cctaatgaca 6721 ttattggaat tgtttcattg tcattgccag agtatatatc taagttatca ccgcaaagcc 6781 ttgcaatttc ggaaatctga tcgatattag agcttgcttc cttaacagca acaatatttt 6841 taatttttga aagctcatac aaggtttgag gtaaaagatt aaggcctgtt cttcctggta 6901 cattgtaaat tatgattggc tttgtcacgc tgtttgcaat tgccttaaag tgttctatgc 6961 agcccctttg agtagtcttg ttgtagtaag gcgttattaa taggagggcg tcgactccaa 7021 tactttcagc ccacacactc atttcaacag ctgcctttgt attgttagag cctgttcctg 7081 caataacagg aattcttcca ttgaccttat taactgtata tttaatggtt tctttttttt 7141 catcaaggga cattgtagaa gattctcctg tagtaccgca aattattata gcatcagttt 7201 tttcttttat atgccattct aaaagttccc caagcttttc aaagtcaact ccttctttat 7261 taaaaggagt aacaattgca acaccagaac cagtaaatac actcatatat aaccatcctt 7321 tcttaaatta tatttgtttt attatatgct ttgagattaa atatataaaa ctgcaagcaa 7381 ttattttaat agtaactgag ctatttgaac tgcatttgta gcagcgcctt ttcttatgtt 7441 atctgccact acccaaatat taattccact atcaacgctt tcatcacgtc ttattcttcc 7501 aacataaact tcatcttttc cttcaacagt aattggcata ggataaattt gattttttac 7561 atcatcatat acaataactc caggtgctga atttaatgtt gaaaaaatat ccttcatatc 7621 aaagcttttt tcaaattcaa tattgatgga ttcacagtgc ccataaaata caggtaccct 7681 ggcagtggtt gctgtaattc ttaaactatc attatgaagt atttttcttg tttcattaac 7741 cattttaatt tcttccttgg tgtatccatt ttccataaat gaatctatgt gtggaagtac 7801 attataggca attgggtaag gaaacttttt gggctgttct ccttttacac catttacaag 7861 gtctgaatat ccaacttggc ctgctcctga aacagcttgg tatgtggagt atacaatcct 7921 tctaatatta aatttatcat ttagcggctt taaggctacc acagcttgaa tagtagagca 7981 gttaggattt gcaattatac ccttattcca gcttatatcc tcagggttaa cttcaggtac 8041 aacaagggga acatttttat ccattctcca tgcactgctg ttgtctacaa cgatacatcc 8101 cttttgtgat gctattggtg caaattttaa acttgttgat gcgcctgctg aaaatagtgc 8161 aatatctatt cctctatcaa aagaatcctc ttttagttct tctactgtat attccttatc 8221 tttaaacatc atagtttttc ctgcggattt ttgtgaggca aataagtaaa gcttatcaat 8281 aggaaagttc ttttcttgta aaacttcaag aatctttctt ccaactaatc cagtggcgcc 8341 tactactgca actttaactc ccattttaac tcctccttat taatataatt tattaataaa 8401 tgtttaaaaa atttacatta taatgagatt ttcaagtatt tatttattgt aatgtattat 8461 agtattatat tgtattatag cataaaaatt ttaattttta taaatgaatt tgcaggacta 8521 aaacttatca ttaagaaata ttaaattact aaataatcaa tgaataaaaa acatatattt 8581 acagataata atttttggtg atagtaatat gacaactcca tttaaaaaag tggtaaaagc 8641 aaaaataaaa gcaaagaagg agctctcacc tgtagaaaag gaaagagagc tcataaaata 8701 tgaaattgca gaagaattag ggcttaagga cagggtggaa aaatacggct ggagtggact 8761 tacagcagag gaaacgggca ggattggcgg aataatgacg aaaatgaata aggatgcagg 8821 cagaaggtgg aatgactagt taaatataag attttaaaag ctgtaagttt ccagcaattt 8881 caaattctaa attgtccgct ggaattaaaa agcttgttcc accttttata tcaaaataat 8941 tatccttata tttcagtttt ccattgcctt ctacgcatgt ataggcaaaa aatgtgctgc 9001 catctgtttt atccttgtag ctattaaata catgtatttt gtcaacatta aagaatttac 9061 agtttatgag gcttgtatgt tcaaagcctt cttctttttt tgttgaaact ttttcaactg 9121 agcctttgta ttcaaaattt attacatcca gtgccttttc tacatgaagc tccctgggct 9181 tgccatcttt gcctacacgt ccccagtcat aaactctata ggtagtatca ctgttttgct 9241 gtatttctgc tataactatt ccatctaaaa tagcatgaac agtaccagat ggaataaaaa 9301 ttgtatctcc ctttttaaca tccacataat ttagatattc atttagtgta ttgtttttta 9361 ttgcttcttc aaattgtttt tttgtagttc cactttttac accacaaata agtttggcat 9421 taggtttagc atcaatgatg taccacattt ctgtttttcc taaatcattg tcgtgtattt 9481 ttgcatattc attatcagga tgtacttgaa cagaaagctt atcgtttgca tcaattaatt 9541 taattaaaag aggaaatctt tctgatgagc attttttgcc aacaagttta ctgccaaatt 9601 tattaagaag ttcattgaga cttttaccct taaactcacc atttgtgatt atgctgttat 9661 ctttgccgtg attgcttatt tcccagcttt ctgctacctt cccttcaggt aaacttctat 9721 taaatatcct ttctaaattt ctgccacccc atattatact tttatatatt ggatcaaaaa 9781 ataatggttc catgtttatc tcctttcaaa acttatgctt taattataat ctatcacatt 9841 atcataagaa tacaaaaaag ctacgcaaaa aatgcgcagc tttttcttct ttttatttta 9901 gccgttttct tttttgttct gcaaagggga aagcagttcc ttacaggttc ttttcgcaga 9961 agtccttcaa tgctgcagca tccttggcct cactggcgcc ttccacagaa aaggtaattt 10021 cattgttttt cgtaacaccg aggcccatga ctgctaaaat actctttgcg tctgcactgt 10081 gaccattggc agccatctgt acagaagatg tacacttctg tgcgcattta actaaaagtc 10141 cagctggacg agcgtgaagg ccctgcggat cggtaatggt atagatgaat ttctgcatat 10201 caaccacttc tattctataa atataaaatt tgtttattaa aaattgagct ttcaattaga 10261 ctactttgct tgcgaattct ttcttctgtt ttatgccttt tagggctata accatcagcg 10321 tggaaataac tgtgcccgca gcaatcgtta gtaggaacag tggtagactg ttggttgcac 10381 ccaagaaacc gacaattgga ccgccatgcg gaactatatc tgtaatgcca agaaccatgc 10441 ctagtgccga agcaacactg ctgccaacga taatgctggg catgacatgc tttgggtcac 10501 aggcagcata cggaatggca ccttcagtaa tgccaataag tcccatcagc agtgctggaa 10561 tgccagcagc acgttcttct tcattaaaac atttcttatc gattagcgtt gccaatccca 10621 ttgccaatgg aggcactgga atagcacacg caactgcacc cataatctgc ggtgtacctc 10681 caacaatgga ggctgtacca aataggaatg caaccttgtt gactgggcca cccatatcga 10741 aagcaaccat agcaccaagc agcagaccaa gcggaatggc tgttactgga tctttagaca 10801 ggaaagtcag tgcactgttc agggcagtca tcagccagga aattggtgca ccaaggaaca 10861 gaatcaatac ggccgaaact gctgctgttc ccaaaagggg aattacaaaa attggcataa 10921 ttggtttcag gctccttggt atcttccaac tgttcatcca tttgactaga taaccggtca 10981 ggtagccaac aaaaatagcg ccgaggaatc cggtgccggc ggatgtgcca agaatttcct 11041 tgctgtttgc aaccactgcg gaaatcatgg ccggtgctag tgctgcacga ccagagattg 11101 cataagcaat atagcccgcc agaatcggaa tcatcagatt aaagccaatg ccacctatct 11161 ggtttacttt atcccaagcc cccggtggaa tgaccatgcc ctttgctgtg ggcttaccgc 11221 cgatggaaat ggatatagcg ataaacaggc caccgacaac gacgaatgga atcatgtagg 11281 aaacaccgtt catcagctgc ttcatggctg ttttgcctgc actgtcgcca gaggattctt 11341 tcttctcctt ttcggtctcc ttggtgactt ccttactgct gctgtaagta tgtgcacgtg 11401 ttggc //