LOCUS       TTHAQUI                 2173 bp    DNA     linear   BCT 25-APR-2009
DEFINITION  Thermus aquaticus aquI gene for aqualysin precursor, complete cds.
ACCESSION   D90108 J05414
VERSION     D90108.1
KEYWORDS    .
SOURCE      Thermus aquaticus
  ORGANISM  Thermus aquaticus
            Bacteria; Deinococcota; Deinococci; Thermales; Thermaceae; Thermus.
REFERENCE   1  (bases 528 to 1632)
  AUTHORS   Kwon,S.T., Terada,I., Matsuzawa,H. and Ohta,T.
  TITLE     Nucleotide sequence of the gene for aqualysin I (a thermophilic
            alkaline serine protease) of Thermus aquaticus YT-1 and
            characteristics of the deduced primary structure of the enzyme
  JOURNAL   Eur. J. Biochem. 173, 491-497 (1988)
REFERENCE   2  
  AUTHORS   Terada,I., Kwon,S.T., Miyata,Y., Matsuzawa,H. and Ohta,T.
  TITLE     Unique precursor structure of an extracellular protease, aqualysin
            I, with NH2- and COOH-terminal pro-sequences and its processing in
            Escherichia coli
  JOURNAL   J. Biol. Chem. 265, 6576-6581 (1990)
COMMENT     These data kindly submitted in computer readable form by:
            Hiroshi Matsuzawa
            Department of Agricultural Chemistry,
            The University of Tokyo
            Bunkyo-ku, Tokyo 113
            Japan
FEATURES             Location/Qualifiers
     source          1..2173
                     /db_xref="taxon:271"
                     /mol_type="genomic DNA"
                     /organism="Thermus aquaticus"
                     /strain="YT-1"
     regulatory      248..253
                     /regulatory_class="minus_35_signal"
     regulatory      272..277
                     /regulatory_class="minus_10_signal"
     regulatory      297..302
                     /note="ribosome-binding sequence"
                     /regulatory_class="ribosome_binding_site"
     CDS             306..1847
                     /codon_start=1
                     /EC_number="3.4.21.62"
                     /gene="aquI"
                     /note="aa 1 to 513"
                     /product="aqualysin precursor"
                     /protein_id="BAA14135.1"
                     /transl_table=11
                     /translation="MRKTYWLMALFAVLVLGGCQMASRSDPTPTLAEAFWPKEAPVYG
                     LDDPEAIPGRYIVVFKKGKGQSLLQGGITTLQARLAPQGVVVTQAYTGALQGFAAEMA
                     PQALEAFRQSPDVEFIEADKVVRAWATQSPAPWGLDRIDQRDLPLSNSYTYTATGRGV
                     NVYVIDTGIRTTHREFGGRARVGYDALGGNGQDCNGHGTHVAGTIGGVTYGVAKAVNL
                     YAVRVLDCNGSGSTSGVIAGVDWVTRNHRRPAVANMSLGGGVSTALDNAVKNSIAAGV
                     VYAVAAGNDNANACNYSPARVAEALTVGATTSSDARASFSNYGSCVDLFAPGASIPSA
                     WYTSDTATQTLNGTSMATPHVAGVAALYLEQNPSATPASVASAILNGATTGRLSGIGS
                     GSPNRLLYSLLSSGSGSTAPCTSCSYYTGSLSGPGDYNFQPNGTYYYSPAGTHRAWLR
                     GPAGTDFDLYLWRWDGSRWLTVGSSTGPTSEESLSYSGTAGYYLWRIYAYSGSGMYEF
                     WLQRP"
     sig_peptide     306..347
                     /note="aa 1 to 14"
     mat_peptide     687..1529
                     /note="aa 128 to 408"
                     /product="aqualysin"
     regulatory      1862..1888
                     /note="transcription termination signal"
                     /regulatory_class="terminator"
BASE COUNT          361 a          739 c          680 g          393 t
ORIGIN      
        1 cctaggacgg gatcgcccgg gccatccgcc ccgcccacac ccccctggac ggggacctgg
       61 tcttcgccct ggccctgggg gagggcaggg gggtggaccc ctacctcctc ctccgcctcg
      121 gggcctacgc cgccgacgcc ctcgcgcggg ccatgcccgg gcggtgctgt tggcggaggg
      181 gatgttgggg gtgcctgcat accggcagct tgtgcgtaaa aatgaagaat aactaataaa
      241 aacccccttg acacccgggc atccttaggg ttagctttgc cctcgtgaaa tccacaaagg
      301 agcgtatgag gaagacttat tggctgatgg cgcttttcgc ggtgctcgtt ttgggtggtt
      361 gtcagatggc ctcccgctcc gatccaaccc ctaccttggc tgaggccttc tggcccaagg
      421 aggctcccgt ctatggcctg gatgaccctg aagctatccc gggccggtac attgtggtct
      481 ttaagaaggg gaagggtcag tctctgctcc aaggtggcat cacaaccctg caggcacggc
      541 tggctcctca gggggtagtg gtgacccagg cctacacggg cgccctccag ggatttgcgg
      601 cggagatggc gccccaggcc ttagaggcct ttaggcagag tcccgacgtg gagttcatag
      661 aggcggacaa ggtggtacgg gcctgggcta cccagagccc ggctccttgg ggcctggacc
      721 ggattgacca gcgggacctt cccctttcca acagctacac ctacaccgct acgggaaggg
      781 gggttaacgt ctatgtgatt gacaccggaa tccgcacgac ccaccgggag ttcggcggcc
      841 gggcccgggt aggctatgac gccttagggg ggaacggcca ggactgcaac ggccacggta
      901 cccatgtggc gggaacgatc ggcggagtga cctatggggt agccaaagcg gttaacctct
      961 acgctgtgcg cgtcctggac tgcaacggtt ccggctccac ctctggggtc atcgctgggg
     1021 tggactgggt cacgcggaac caccgcaggc cggccgttgc caacatgagc ttaggaggcg
     1081 gagtctccac tgccctggac aacgccgtga agaactccat cgccgcggga gtggtctacg
     1141 ctgtggctgc ggggaacgac aacgccaacg cctgcaacta ctccccagcc cgggtggccg
     1201 aggcccttac cgtgggcgct accacatctt ccgacgcccg tgccagcttc tccaactacg
     1261 gtagttgcgt ggacctcttc gcccctgggg cttccattcc ctcggcgtgg tacacctcgg
     1321 acacggccac ccagaccctt aacggcacct ccatggccac cccccatgtg gccggggtgg
     1381 ctgctttgta tctagagcaa aatccttcgg ctacgccggc ctctgttgct agcgctatcc
     1441 tcaacggagc cactacgggg cggctttcgg ggatcggatc ggggtccccc aaccgtctcc
     1501 tttactccct gctctcctcg gggagtggtt ccacagcccc ctgtaccagc tgtagctact
     1561 acacgggcag cctttccggt cctggtgact ataacttcca acccaacggc acctactact
     1621 acagccctgc aggtacccat agggcctggc ttaggggccc cgccggaacg gactttgacc
     1681 tctacctctg gcggtgggac ggctcccgtt ggctgaccgt gggtagctct acggggccca
     1741 cctcggagga aagtctcagc tacagcggaa ctgctggcta ctacctctgg cgcatctacg
     1801 cctatagcgg ctcggggatg tacgagttct ggctccagcg cccctaggcg aaggagttct
     1861 tcctcccctg ggaagcgcct gggggaggtt ttccctttag cgtcctggga aaggggcgag
     1921 gaccgcttcc acctggaaga cctggccccc ccggcgcacg gtgagggcga tccggccccc
     1981 cacctggtgg cggcgcacct cgcggaggag gtcctcaaag gagttcaccg gcaccccgtt
     2041 cacctccagg atcacgtcgg gcacgcctcc cgcctcgagg cccctaagcc ccgcccggtg
     2101 ggccgccccg cccggcacca cctcccccac caggaccccc gcccggcggg aggcccagct
     2161 cccgggcgag ctc
//