LOCUS       D86930                  1120 bp    mRNA    linear   PLN 06-JUN-2003
DEFINITION  Glycine max URnod-35 mRNA for uricase (Nod-35), complete cds.
ACCESSION   D86930
VERSION     D86930.1
KEYWORDS    .
SOURCE      Glycine max (soybean)
  ORGANISM  Glycine max
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
            Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae;
            50 kb inversion clade; NPAAA clade; indigoferoid/millettioid
            clade; Phaseoleae; Glycine; Glycine subgen. Soja.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Tajima,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-1996)
            Contact:Shigeyuki Tajima
            Kagawa university, Faculty of Agriculture; Ikenobe 2393, Miki,
            Kagawa 761-07, Japan
REFERENCE   2  
  AUTHORS   Takane,K., Tanaka,K., Tajima,S., Okazaki,K. and Kouchi,H.
  TITLE     Expression of uricaseII gene (Nod-35) in cotyledons of soybean
            plants.
  JOURNAL   Unpublished
REFERENCE   3  
  AUTHORS   Nguyen,T., Zelechowska,M., Foster,H., Bergmann,H. and Verma,D.P.
  TITLE     Primary structure of the soybean noduli-35 gene encoding uricase
            II localized in the peroxisomes of uninfected cells of nodules.
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 82, 5040-5044 (1985)
COMMENT     
FEATURES             Location/Qualifiers
     source          1..1120
                     /clone="URnod-35"
                     /cultivar="Akisengoku"
                     /db_xref="taxon:3847"
                     /mol_type="mRNA"
                     /organism="Glycine max"
                     /tissue_type="nodule"
     CDS             7..936
                     /codon_start=1
                     /EC_number="1.7.3.3"
                     /gene="URnod-35"
                     /product="uricase (Nod-35)"
                     /protein_id="BAA13185.1"
                     /translation="MAKQEVVEGFKFEQRHGKERVRVARVWKTRQGQHFIVEWRVGIT
                     LFSDCVNSYLRDDNSDIVATDTMKNTVYAKAKECSDILSAEEFAILLAKHFVSFYQKV
                     TGAIVNIVEKPWERVTVDGQPHEHGFKLGSEKHTTEAIVQKSGSLQLTSGIEGLSVLK
                     TTQSGFVNFIRDKYTALPDTRERMVATEVTALWRYSYESLYSLPQKPLYFTEKYQEVK
                     KVLADTFFGPPKGGVYTPSVQNTLYLMAKATLNRFPDIAYVSLKLPNLHFIPVNISNQ
                     DGPIVKFEDDVYLPTDEPHGSIQASLSRLWSKL"
BASE COUNT          307 a          226 c          275 g          312 t
ORIGIN      
        1 gaaaagatgg ctaagcagga agtggtggaa gggttcaagt tcgagcagag gcacgggaaa
       61 gaacgcgtga gagtggcgcg cgtgtggaag acgaggcagg ggcagcactt cattgtggag
      121 tggcgcgtgg ggatcactct cttttcggat tgcgtcaact cgtacctccg cgatgacaac
      181 tctgacatcg ttgctactga taccatgaaa aacaccgtgt atgcaaaagc aaaggaatgc
      241 tctgacatac tttctgccga ggagtttgct attctgcttg ctaagcactt tgtatcattt
      301 taccagaagg ttactggtgc tattgtgaat attgtggaaa aaccatggga gcgtgtcact
      361 gtggatggtc aacctcatga acatggtttc aaacttgggt ctgagaagca tacaacagag
      421 gcgatagtac aaaagtctgg ttcacttcag ttgacttctg gtattgaagg attgtcagtg
      481 ttgaagacaa cccagtctgg ttttgtgaat ttcataagag acaagtacac agcacttcct
      541 gatacccgtg aaaggatggt agcaacagaa gtaaccgcac tgtggaggta ttcgtatgaa
      601 tcgctgtata gcctccctca gaagccgctt tactttacag aaaagtatca ggaagtgaaa
      661 aaagttctgg ctgacacttt ttttggccca ccaaaagggg gagtctatac gccatctgtt
      721 caaaacacac tctacctgat ggcaaaggcc acactgaaca gatttcctga catagcttat
      781 gtcagtctaa agttgccaaa tcttcatttc atacctgtca atatctcaaa ccaggatggc
      841 cctattgtga agtttgagga tgatgtgtac ttgccaacgg atgagccaca tgggtcaatt
      901 caagctagtt tgagccgcct ttggtcaaag ctgtagaaac tgcagaaact atggtgctca
      961 tcccttctgg tggattgcta gtgcagccta aactcgcaaa cctggcctca gataatgctt
     1021 tccattaatg tgtcttgtaa ttctggtttt tgttatcttt atcttttgtt ttgtttcatg
     1081 aagaactatt atgcatgaat aagttccagg agggatattc
//