LOCUS       ENEGE1E                 1827 bp    DNA     linear   BCT 14-MAR-2006
DEFINITION  Enterococcus faecalis  gelatinase (gelE) DNA, complete cds.
ACCESSION   D85393
VERSION     D85393.1
KEYWORDS    .
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Kirimura,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-1996) to the DDBJ/EMBL/GenBank databases.
            Contact:Kohtaro Kirimura
            Waseda University, School of Science and Engineering; 3-4-1
            Ohkubo, Shinjuku-ku, Tokyo 169, Japan
REFERENCE   2  
  AUTHORS   Kirimura,K., Ebihara,T., Kamigaki,K. and Usami,S.
  TITLE     Development of host-vector system for Enterococcus faecalis and
            Escherichia coli, and expression of neopullulanase gene from
            Bacillus stearothermophilus in E. faecalis.
  JOURNAL   Unpublished (1996)
COMMENT     
FEATURES             Location/Qualifiers
     source          1..1827
                     /clone="pEFZG3"
                     /db_xref="taxon:1351"
                     /mol_type="genomic DNA"
                     /organism="Enterococcus faecalis"
                     /strain="IFO3989"
                     /sub_species="zymogenes"
     regulatory      72..77
                     /regulatory_class="minus_35_signal"
     regulatory      97..102
                     /regulatory_class="minus_10_signal"
     regulatory      215..218
                     /regulatory_class="ribosome_binding_site"
     CDS             230..1759
                     /gene="gelE"
                     /product="gelatinase"
                     /protein_id="BAA12802.1"
                     /transl_table=11
                     /translation="MKGNKILYILGTGIFVGSSCLFSSLFVAAEEQVYSESEVSTVLS
                     KLEKEAISEAAAEQYTVVDRKEDAWGMKHLKLEKQTEGVTVDSDNVIIHLDKNGAVTS
                     VTGNPVDQVVKIQSVDAIGEEGVKKIIASDNPENKDLVFLAIDKRVNNEGQLFYKVRV
                     TSSPTGDPVSLVYKVNATDGTIMEKQDLTEHVGSEVTLKNSFQVTFNVPVEKSNTGIA
                     LHGTDNTGVYHAVVDGKNNYSIIQAPSLATLNQNAVDAYTHGKFVKTYYEDHFQRHSI
                     DDRGMPILSVVDEQHPDAYDNAFWDGKAMRYGETSTPTGKTYASSLDVVGHEMTHGVT
                     EHTAGLEYLGQSGALNESYSDLMGYIISGASNPEIGADTQSVDRKTGIRNLQTPSKHG
                     QPETMAQYDDRARYKGTPYYDQGGVHYNSGIINRIGYTIIQNLGIEKAQTIFYSSLVN
                     YLTPKAQFSDARDAMLAAAKVQYGDEAASVVSAAFNSAGIGAKEDIQVNQPSESVLVN
                     E"
BASE COUNT          632 a          274 c          409 g          512 t
ORIGIN      
        1 aacaactttt ttgctatggt attgagttat gaggggcaat acagggaaaa atgtcggctg
       61 attaaggaat ttagatagtg ccggttagta gttgtctata atgaaaatag caacaaatat
      121 ttacgcaggg aaaggggcgg tcgtttaacg ggaaaaatta gggaggataa agcaatactt
      181 ttgttgggaa aagaaataaa aggaaactgg ggaaggagtt aattgtttga tgaagggaaa
      241 taaaatttta tacattttag gtacaggcat ctttgttgga agttcatgtc tattttcttc
      301 actttttgta gccgcagaag aacaagttta ttcagaaagt gaagtttcaa cagttttatc
      361 gaagttggaa aaggaggcaa tttctgaggc agctgctgaa caatatacgg ttgtagatcg
      421 aaaagaagac gcgtggggga tgaagcatct taagttagaa aagcaaacgg aaggcgttac
      481 tgttgattca gataatgtga ttattcattt agataaaaac ggtgcagtaa caagtgttac
      541 aggaaatcca gttgatcaag ttgtgaaaat tcaatcggtt gatgcaatcg gtgaagaagg
      601 agttaaaaaa attattgctt ctgataatcc ggaaaataaa gatcttgtct ttttagctat
      661 tgacaaacgt gtaaataatg aagggcaatt attttataaa gtcagagtaa cttcttcacc
      721 aactggtgac cccgtatcat tggtttataa agtgaacgct acagatggaa caattatgga
      781 aaaacaagat ttaacggaac atgtcggtag tgaagtaacg ttaaaaaact cttttcaagt
      841 aacgtttaat gtaccagttg aaaaaagcaa tacgggaatt gctttacacg gaacggataa
      901 cacaggggtt taccatgcag tagttgatgg caaaaataat tattctatta ttcaagcgcc
      961 atcactagcg acattaaatc agaatgctgt tgacgcctat acgcatggaa aatttgtgaa
     1021 aacatattat gaagatcatt tccaacgaca cagtattgat gatcgaggga tgcccatctt
     1081 gtcagttgtt gatgaacaac atccagatgc ttatgacaat gctttttggg atggaaaagc
     1141 aatgcgttat ggtgaaacaa gtacaccaac aggaaaaacg tatgcttcct ctttagatgt
     1201 agttggtcat gaaatgacac atggtgtgac ggaacatact gccggtttag aatatttagg
     1261 acaatcaggt gccttgaatg aatcttattc tgatttgatg ggttatatta tttcgggtgc
     1321 atctaatcca gaaattggtg cggatactca gagtgttgac cgaaaaacag gtattcgaaa
     1381 tttacaaacg ccaagtaaac acggacaacc agaaaccatg gctcaatacg acgatcgagc
     1441 acggtataaa ggaacgcctt attatgatca aggcggtgtt cattataata gtggaattat
     1501 taatcggatt ggttacacca ttatccagaa cttaggcatt gaaaaagcac agactatttt
     1561 ctacagctcg ttagtaaatt acttaacacc taaagcacaa ttcagtgatg ctcgtgatgc
     1621 gatgcttgct gctgcaaaag ttcaatatgg cgatgaagca gcttcagtgg tgtcagcagc
     1681 ctttaactct gctggaatcg gagctaaaga ggatattcaa gtaaaccaac caagtgaatc
     1741 tgttctggtc aatgaatgaa aaaaattccc caattaaata aaaatggata aggagtggca
     1801 taatcttatg aaaaagttct ccatacg
//