LOCUS       ASNAI                   1773 bp    DNA     linear   PLN 01-FEB-2003
DEFINITION  Aspergillus saitoi gene for aspergillopepsin I, complete cds.
ACCESSION   D25318
VERSION     D25318.1
KEYWORDS    .
SOURCE      Aspergillus saitoi
  ORGANISM  Aspergillus phoenicis
            Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
            Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae;
            Aspergillus.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Ichishima,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-NOV-1993) to the DDBJ/EMBL/GenBank databases.
            Contact:Eiji Ichishima
            Tohoku University, Department of Agriculture; 1-1,
            Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, Miyagi 981, Japan
REFERENCE   2  
  AUTHORS   Shintani,T. and Ichishima,E.
  TITLE     Primary structure of aspergillopepsin I deduced from nucleotide
            sequence of the gene and aspartic acid-76 is an essential active
            site of the enzyme for trypsinogen activation
  JOURNAL   Biochim. Biophys. Acta 1204, 257-264 (1994)
REFERENCE   3  
  AUTHORS   Shintani,T., Kobayashi,M. and Ichishima,E.
  TITLE     Characterization of the S1 subsite specificity of Aspergillopepsin
            I by site-directed mutagenesis
  JOURNAL   J. Biochem. 120, 974-981 (1996)
REFERENCE   4  
  AUTHORS   Shintani,T., Nomura,K. and Ichishima,E.
  TITLE     Engineering of porcine pepsin
  JOURNAL   J. Biol. Chem. 272, 18855-18861 (1997)
COMMENT     
FEATURES             Location/Qualifiers
     source          1..1773
                     /clone="apnS"
                     /db_xref="taxon:5063"
                     /mol_type="genomic DNA"
                     /organism="Aspergillus saitoi"
     CDS             join(289..608,660..937,990..1238,1291..1628)
                     /codon_start=1
                     /EC_number="3.4.23.18"
                     /note="trypsinogen kinase"
                     /product="aspergillopepsin I"
                     /protein_id="BAA04988.1"
                     /translation="MVVFSKTAALVLGLSTAVSAAPAPTRKGFTINQIARPANKTRTV
                     NLPGLYARSLAKFGGTVPQSVKEAASKGSAVTTPQNNDEEYLTPVTVGKSTLHLDFDT
                     GSADLWVFSDELPSSEQTGHDLYTPSSSATKLSGYSWDISYGDGSSASGDVYRDTVTV
                     GGVTTNKQAVEAASKISSEFVQDTANDGLLGLAFSSINTVQPKAQTTFFDTVKSQLDS
                     PLFAVQLKHDAPGVYDFGYIDDSKYTGSITYTDADSSQGYWGFSTDGYSIGDGSSSSS
                     GFSAIADTGTTLILLDDEIVSAYYEQVSGAQESYEAGGYVFSCSTDLPDFTVVIGDYK
                     AVVPGKYINYAPVSTGSSTCYGGIQSNSGLGLSILGDVFLKSQYVVFNSEGPKLGFAA
                     QA"
     exon            <289..608
                     /number=1
     intron          609..659
                     /number=1
     exon            660..937
                     /number=2
     intron          938..989
                     /number=2
     exon            990..1238
                     /number=3
     intron          1239..1290
                     /number=3
     exon            1291..>1628
                     /number=4
BASE COUNT          366 a          515 c          458 g          434 t
ORIGIN      
        1 ttggacggac gtgcggggat cttcgtcggc ttattcctgg aagggacatt atctccttcc
       61 atcatcgcgg ctgccatggc ggggactgag acattttgct ctgaagagca tggtcgactt
      121 ggatgatgga tgagttgatc gaggtcaatg gggagggttt gcaagtataa gaagagagtg
      181 ctcgaccagc aaaatgtctc ttcttgttca tcaaccaaga gtctaaggct tctttgtctg
      241 gttctatctc ttctccgaac tctcttgctt gacattctcg tggtcaaaat ggtcgtcttc
      301 agcaaaaccg ctgccctcgt tctgggtctg tccaccgccg tctctgcggc accggctccc
      361 actcgcaagg gcttcaccat caaccagatt gcccggcctg ccaacaagac ccgcaccgtc
      421 aacttgccgg gtttgtatgc ccgttccctg gccaagtttg gcggtacggt gccccagagc
      481 gtgaaggagg ctgccagcaa gggtagtgcc gtgaccacgc cccagaacaa tgacgaggag
      541 tacctgactc ccgtcactgt cggaaagtcc acccttcatc tggactttga caccggatct
      601 gcagatctgt aagattcccc gcgaatgtgc tcgtacgaac cgtcactaac ctgaaccagc
      661 tgggtcttct cagacgagct cccttcctcg gagcagaccg gtcacgatct gtacacgcct
      721 agctccagcg cgaccaagtt gagcggctac tcttgggaca tctcttacgg tgacggcagc
      781 tcggccagcg gagacgtcta ccgggatact gtcactgttg gcggtgtcac caccaacaag
      841 caggccgttg aagctgccag caagatcagc tccgagttcg ttcaggacac ggccaatgat
      901 ggtcttctgg gtctggcctt cagctccatc aacactggtg agtaaagcct acatcagctg
      961 ggttgactta cctgctgacc gatatgcagt ccagcccaag gcacagacca ccttcttcga
     1021 caccgtcaag tctcagctgg actctcctct tttcgccgtg cagttgaagc acgacgcccc
     1081 tggtgtctac gactttggct acatcgatga ctccaagtac accggttcca tcacctacac
     1141 ggatgccgat agctcccagg gctactgggg cttcagcacc gacggctaca gcatcggcga
     1201 cggcagctcc agctccagcg gcttcagcgc cattgctggt aagagccctt ttcagattct
     1261 cttgctttct ctttactgat tagtgtatag acaccggtac caccctcatc ctcctcgacg
     1321 atgagatcgt ttccgcctac tacgagcagg tttccggcgc ccaggagagc tatgaagctg
     1381 gtggctacgt tttctcttgc tctactgacc ttcctgactt caccgtcgtg atcggcgact
     1441 acaaggccgt cgttcctggc aagtacatca actacgctcc cgtttcgacc ggcagctcca
     1501 cctgctacgg cggtatccag agcaacagcg gtctcggact gtccatcctg ggtgatgtgt
     1561 tcttgaagag ccagtacgtg gtcttcaact ctgagggacc taagctgggc tttgctgctc
     1621 aggcttagat caacactgaa aaagtggggt attcgatctg ctgatcgatc cctcctcggt
     1681 gggatacatg tggacatgta tagcaaacga tggtcatggt gatgatgttg atattgatcc
     1741 ttgatgatga accgtacata tttgatgaag ctt
//