LOCUS       CT010328                1173 bp    mRNA    linear   ROD 24-SEP-2008
DEFINITION  Mus musculus full open reading frame cDNA clone RZPDo836E0552D for
            gene Daf1, Decay accelerating factor 1; complete cds, incl.
            stopcodon.
ACCESSION   CT010328
VERSION     CT010328.1
KEYWORDS    Full ORF clone, Expression Shuttle System, Gateway(R), complete
            cds.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Ebert L., Muenstermann E., Schatten R., Henze S., Bohn E.,
            Mollenhauer J., Wiemann S., Schick M., Korn B.
  TITLE     Cloning of mouse full open reading frames in Gateway(R) system
            entry vector (pDONR201)
  JOURNAL   Unpublished.
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Ebert L., Muenstermann E., Schatten R., Henze S., Bohn E.,
            Mollenhauer J., Wiemann S., Schick M., Korn B.
  JOURNAL   Submitted (20-JUL-2005) to the INSDC. RZPD Deutsches
            Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
            515, D-69120 Heidelberg, Germany
COMMENT     RZPD; RZPDo836E0552D, ORFNo 30305
            http://www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo836E0552D
            Mouse Full ORF Gateway(R) Clones - RZPD (kan-resist.)
            RZPDLIB No. 836
            http://www.rzpd.de/cgi-bin/products/showLib.pl.cgi/
            response?libNo=836
            http://www.rzpd.de/products/orfclones/
            Contact: Inge Arlart
            RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
            Heubnerweg 6, D-14059 Berlin, Germany
            Tel: +49 30 32639 100
            Fax: +49 30 32639 111
            http://www.rzpd.de
            This clone is available from RZPD;
            Contact RZPD (customer.service@rzpd.de) for further information.
            
            This CDS clone is part of a collection of mouse full ORF clones
            jointly established and verified by DKFZ (www.dkfz.de) and RZPD.
            This CDS has been cloned incl. stopcodon.
            The CDS has been inserted into pDONR201 via a BP Clonase(TM)
            reaction.
            Additional sequence has been added in front of the start codon:
            att..AAAAAA GCA GGC TCC ACC (ATG).
            The stopcodon is followed by the 3' att site:
            GACCCAGCTTTCTTGTAC..att
            The clone is validated by full sequence check.
            Compared to the reference sequence BC011314 (GI:15030122)
            we found AA exchange(s) at position (first base of changed
            triplet):
            292(leu->cys) 343(his->asn) 937(leu->thr)
            Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Mus musculus"
                     /lab_host="DH10B"
                     /mol_type="mRNA"
                     /clone_lib="Mouse Full ORF Gateway(R) Clones - RZPD"
                     /clone="RZPDo836E0552D"
                     /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
                     /db_xref="taxon:10090"
     CDS             1..1173
                     /codon_start=1
                     /gene="Daf1"
                     /db_xref="GOA:Q61475"
                     /db_xref="InterPro:IPR000436"
                     /db_xref="InterPro:IPR035976"
                     /db_xref="MGI:MGI:104850"
                     /db_xref="UniProtKB/Swiss-Prot:Q61475"
                     /protein_id="CAJ18536.1"
                     /translation="MIRGRAPRTRPSPPPPLLPLLSLSLLLLSPTVRGDCGPPPDIPN
                     ARPILGRHSKFAEQSKVAYSCNNGFKQVPDKSNIVVCLENGQWSSHETFCEKSCVAPE
                     RLSFASLKKEYLNMNFFPVGTIVEYECRPGFRKQPPLPGKSTCLEDLVWSPVAQFCKK
                     KSCPNPKDLDNGHINIPTGILFGSEINFSCNPGYRLVGVSSTFCSVTGNTVDWDDEFP
                     VCTEIHCPEPPKINNGIMRGESDSYTYSQVVTYSCDKGFVLVGNASIYCTVSKSDVGQ
                     WSSPPPRCIEKSKVPTKKPTINVPSTGTPSTPQKPTTESVPNPGDQPTPQKPSTVKVS
                     ATQHVPVTKTTVRHPIRTSTDKGEPNTGGDRYIYGHTCLITLTVLHAMLSLIGYLT"
BASE COUNT          350 a          289 c          247 g          287 t
ORIGIN      
        1 atgatccgtg ggcgggcgcc taggactcgg ccatcaccgc cgcctccgct gctgccgttg
       61 ctgtcgctgt ctctgttgct gctgtcccca actgtacgcg gagactgcgg cccacctcca
      121 gacattccta atgccaggcc aatcttgggc agacactcca agtttgctga gcaaagcaaa
      181 gtggcatact cgtgtaataa cggctttaaa caagttccag acaagtcaaa catagttgtc
      241 tgtcttgaaa atggccaatg gtcgagccac gaaacattct gtgagaaatc atgtgttgct
      301 ccagaaagac tgagttttgc atccctcaaa aaagagtacc tcaacatgaa ttttttccca
      361 gttggtacta ttgtggaata tgagtgtcgg ccaggatttc gaaaacaacc tccactccca
      421 ggaaaatcaa cttgccttga ggatttagta tggtctccag ttgctcagtt ttgtaaaaaa
      481 aaatcatgcc ctaatcctaa agatctggat aatggtcaca tcaacatacc aaccggcata
      541 ttattcggtt cagaaataaa cttctcatgc aacccagggt acaggctagt gggtgtctcc
      601 tctactttct gttctgtcac aggaaatact gttgattggg acgatgagtt tccagtgtgc
      661 acagaaatac attgtccaga gccaccaaaa atcaacaatg gaataatgcg aggggaaagt
      721 gactcttata cgtatagcca ggtggtcacc tattcatgtg acaaaggctt cgtcctggtt
      781 ggaaatgcta gcatttattg tactgtgagc aagtctgatg taggacaatg gagcagtcca
      841 ccaccccggt gcatagagaa atccaaggtc ccaacgaaga aaccaacaat taatgttcca
      901 agtacaggaa ccccctcaac gcctcagaaa cccacaacag aaagtgttcc aaatccagga
      961 gaccaaccaa ctcctcagaa accttccaca gttaaagttt cagcaaccca gcatgtacct
     1021 gttaccaaga caacagtacg tcatccaata agaacatcta cagacaaagg agagcctaac
     1081 acaggtggtg accgttatat atatggacat acatgtttaa taaccttgac agttttgcat
     1141 gcgatgctat cactcattgg ctacttgaca tag
//