LOCUS CT010167 837 bp mRNA linear ROD 21-OCT-2008 DEFINITION Mus musculus full open reading frame cDNA clone RZPDo836A0950D for gene Lyl1, Lymphoblastomic leukemia; complete cds, incl. stopcodon. ACCESSION CT010167 VERSION CT010167.1 KEYWORDS Full ORF clone, Expression Shuttle System, Gateway(R), complete cds. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 837) AUTHORS Ebert L., Muenstermann E., Schatten R., Henze S., Bohn E., Mollenhauer J., Wiemann S., Schick M., Korn B. TITLE Cloning of mouse full open reading frames in Gateway(R) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 837) AUTHORS Ebert L., Muenstermann E., Schatten R., Henze S., Bohn E., Mollenhauer J., Wiemann S., Schick M., Korn B. JOURNAL Submitted (20-JUL-2005) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld 515, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo836A0950D, ORFNo 30078 http://www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo836A0950D Mouse Full ORF Gateway(R) Clones - RZPD (kan-resist.) RZPDLIB No. 836 http://www.rzpd.de/cgi-bin/products/showLib.pl.cgi/ response?libNo=836 http://www.rzpd.de/products/orfclones/ Contact: Inge Arlart RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 100 Fax: +49 30 32639 111 http://www.rzpd.de This clone is available from RZPD; Contact RZPD (customer.service@rzpd.de) for further information. This CDS clone is part of a collection of mouse full ORF clones jointly established and verified by DKFZ (www.dkfz.de) and RZPD. This CDS has been cloned incl. stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon: att..AAAAAA GCA GGC TCC ACC (ATG). The stopcodon is followed by the 3' att site: GACCCAGCTTTCTTGTAC..att The clone is validated by full sequence check. Compared to the reference sequence AK076114 (GI:26345089) we found AA exchange(s) at position (first base of changed triplet): 19(arg->gly) Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..837 /organism="Mus musculus" /lab_host="DH10B" /mol_type="mRNA" /clone_lib="Mouse Full ORF Gateway(R) Clones - RZPD" /clone="RZPDo836A0950D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:10090" CDS 1..837 /codon_start=1 /gene="Lyl1" /db_xref="GOA:P27792" /db_xref="InterPro:IPR011598" /db_xref="InterPro:IPR036638" /db_xref="InterPro:IPR040238" /db_xref="MGI:MGI:96891" /db_xref="UniProtKB/Swiss-Prot:P27792" /protein_id="CAJ18375.1" /translation="MCPPQAGAEVGSAMTEKTEMVCASSPAPAPPSKPASPGPLSTEE VDHRNTCTPWLPPGVPVINLGHTRPIGAAMPTTELSAFRPSLLQLTALGRAPPTLAVH YHPHPFLNSVYIGPAGPFSIFPNSRLKRRPSHSELDLADGHQPQKVARRVFTNSRERW RQQHVNGAFAELRKLLPTHPPDRKLSKNEVLRLAMKYIGFLVRLLRDQTAVLTSGPSA PGSRKPPARRGVEGSARFGAGHRVEAARSQPVLPGDCDGDPNGSVRPIKLEQTSLSPE VR" BASE COUNT 166 a 285 c 248 g 138 t ORIGIN 1 atgtgcccgc cccaggccgg agcagaggtg ggttccgcca tgactgagaa aactgagatg 61 gtatgtgcct ccagcccagc acctgcccct ccctctaagc cggcctcacc tgggcccctt 121 tctacagagg aggtggacca ccgaaacacg tgcactccct ggttgcctcc cggcgtgcca 181 gtgataaacc tgggacacac caggcccata ggggcagcca tgcccaccac agagctcagt 241 gcctttcggc cctccttgct gcagctaact gccttgggaa gagctccacc taccctggct 301 gtacattacc accctcaccc cttcctcaac agtgtctaca ttgggccagc aggacccttc 361 agcatcttcc ctaacagccg gctgaagcgc agaccaagcc atagtgagct ggacttggct 421 gacgggcacc aaccccagaa ggtggctcgg cgagtgttca ccaacagccg tgagcgctgg 481 cggcagcagc acgttaacgg cgccttcgca gagctcagga agctgctgcc gacccacccg 541 cccgaccgga agctgagcaa gaacgaggtg ctgcgcctgg ccatgaagta tataggcttc 601 ctggtgcggc tgctgcgaga ccaaacggct gtgctgacct ccggccccag cgctcccggg 661 tcccgcaagc cacctgcgcg caggggcgtg gagggcagcg cacgcttcgg ggccgggcac 721 agagtagagg ctgcacgctc acagcccgtg cttcctgggg actgtgatgg cgaccccaat 781 gggtcagtga gacccatcaa gttggagcag acatccctga gtcctgaggt gcggtag //