LOCUS       CR450318                 468 bp    mRNA    linear   HUM 17-APR-2005
DEFINITION  Homo sapiens full open reading frame cDNA clone RZPDo834G111D for
            gene RNASE1, ribonuclease, RNase A family, 1 (pancreatic); complete
            cds; without stopcodon.
ACCESSION   CR450318
VERSION     CR450318.1
KEYWORDS    Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
  TITLE     Cloning of human full open reading frames in Gateway(TM) system
            entry vector (pDONR201)
  JOURNAL   Unpublished.
REFERENCE   2  (bases 1 to 468)
  AUTHORS   Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
  JOURNAL   Submitted (18-MAY-2004) to the INSDC. RZPD Deutsches
            Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
            580, D-69120 Heidelberg, Germany
COMMENT     RZPD; RZPDo834G111D, ORFNo 168
            www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834G111D
            RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
            RZPD LIB No. 834
            www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
            www.rzpd.de/products/orfclones/
            Contact: Ina Rolfs
            RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
            Heubnerweg 6, D-14059 Berlin, Germany
            Tel: +49 30 32639 101
            Fax: +49 30 32639 111
            www.rzpd.de
            This clone is available from RZPD;
            contact RZPD (customer.service@rzpd.de) for further information.
            
            This CDS clone is a part of a collection of human full length
            expression clones generated by RZPD.
            This CDS has been cloned without stopcodon.
            The CDS has been inserted into pDONR201 via a BP Clonase(TM)
            reaction.
            Additional sequence has been added in front of the start codon
            (ATG):
            att..AAAAAA GCT GGC ACC CCT GGT CCA GGT (ATG)
            After the last codon additional sequence has been added:
            CCA GGC CCA GGC GGC G in front of the 3' att site (AC CCA GCT TTC
            TT).
            Compared to the reference sequence NM_002933 we found amino acid
            exchange(s) at position (first base of changed triplet):
            199(arg->leu)
            Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES             Location/Qualifiers
     source          1..468
                     /db_xref="H-InvDB:HIT000267227"
                     /organism="Homo sapiens"
                     /lab_host="DH10B"
                     /mol_type="mRNA"
                     /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
                     /clone="RZPDo834G111D"
                     /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
                     /db_xref="taxon:9606"
     CDS             1..>468
                     /codon_start=1
                     /gene="RNASE1"
                     /db_xref="GOA:P07998"
                     /db_xref="H-InvDB:HIT000267227.11"
                     /db_xref="HGNC:HGNC:10044"
                     /db_xref="InterPro:IPR001427"
                     /db_xref="InterPro:IPR023411"
                     /db_xref="InterPro:IPR023412"
                     /db_xref="InterPro:IPR036816"
                     /db_xref="PDB:1DZA"
                     /db_xref="PDB:1E21"
                     /db_xref="PDB:1H8X"
                     /db_xref="PDB:1Z7X"
                     /db_xref="PDB:2E0J"
                     /db_xref="PDB:2E0L"
                     /db_xref="PDB:2E0M"
                     /db_xref="PDB:2E0O"
                     /db_xref="PDB:2K11"
                     /db_xref="PDB:2Q4G"
                     /db_xref="PDB:3F8G"
                     /db_xref="PDB:4KXH"
                     /db_xref="UniProtKB/Swiss-Prot:P07998"
                     /protein_id="CAG29314.1"
                     /translation="MALEKSLVRLLLLVLILLVLGWVQPSLGKESRAKKFQRQHMDSD
                     SSPSSSSTYCNQMMRRRNMTQGLCKPVNTFVHEPLVDVQNVCFQEKVTCKNGQGNCYK
                     SNSSMHITDCRLTNGSRYPNCAYRTSPKERHIIVACEGSPYVPVHFDASVEDST"
BASE COUNT          113 a          137 c          125 g           93 t
ORIGIN      
        1 atggctctgg agaagtctct tgtccggctc cttctgcttg tcctgatact gctggtgctg
       61 ggctgggtcc agccttccct gggcaaggaa tcccgggcca agaaattcca gcggcagcat
      121 atggactcag acagttcccc cagcagcagc tccacctact gtaaccaaat gatgaggcgc
      181 cggaatatga cacaggggct gtgcaaacca gtgaacacct ttgtgcacga gcccctggta
      241 gatgtccaga atgtctgttt ccaggaaaag gtcacctgca agaacgggca gggcaactgc
      301 tacaagagca actccagcat gcacatcaca gactgccgcc tgacaaacgg ctccaggtac
      361 cccaactgtg cataccggac cagcccgaag gagagacaca tcattgtggc ctgtgaaggg
      421 agcccatatg tgccagtcca ctttgatgct tctgtggagg actctacc
//