LOCUS       BV211331                 364 bp    DNA     linear   STS 29-DEC-2004
DEFINITION  BARC146 Wheat var Chinese Spring Genomic DNA Triticum aestivum STS
            genomic, sequence tagged site.
ACCESSION   BV211331
VERSION     BV211331.1
KEYWORDS    STS.
SOURCE      Triticum aestivum (bread wheat)
  ORGANISM  Triticum aestivum
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP
            clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Song,Q.J., Shi,J.R., Singh,S., Fickus,E.W., Costa,J.M., Lewis,J.,
            Gill,B.S., Ward,R. and Cregan,P.B.
  TITLE     Development and mapping of microsatellite (SSR) markers in wheat
  JOURNAL   Theor. Appl. Genet. 110 (3), 550-560 (2005)
   PUBMED   15655666
COMMENT     Synonyms: XBARC146
            Contact: Dr. Perry Cregan
            Soybean Genomics Improvement Laboratory
            United States Department of Agriculture
            BARC-WEST, Bldg. 006, Rm 100, 10300 Baltimore Ave., MD 20705, USA
            Tel: 301-504-5070
            Fax: 301-504-5728
            Email: creganp@ba.ars.usda.gov
            Primer A: AAGGCGATGCTGCAGCTAAT
            Primer B: GGCAATATGGAAACTGGAGAGAAAT
            STS size: 130
            PCR Profile:
                    Presoak:         95 degrees C for 3.0 minute(s)
                    Denaturation:    95 degrees C for 45 second(s)
                    Annealing:       52 degrees C for 45 second(s)
                    Polymerization:  72 degrees C for 45 second(s)
                    PCR Cycles:      35
                    Thermal Cycler: Perkin Elmer TC or MJ PTC-225 or MBS
            Satellite 384
            Protocol:
                    Template:       30-100 ng
                    Primer:         each 1.5 uM
                    dNTPs:          each 200 uM
                    Taq Polymerase: 0.05 units/ul
                    Total Vol:      10 ul
            
            Buffer:
                    MgCl2:          2.5 mM
                    KCl:           50 mM
                    Tris-HCl:      10 mM
                    pH:             8.3.
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Triticum aestivum"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:4565"
                     /clone_lib="Wheat var Chinese Spring Genomic DNA"
                     /note="Vector: pBluescript; V-type: Plasmid; Genomic
                     fragments cloned into pBluescript"
     STS             27..156
     primer_bind     27..46
     primer_bind     complement(132..156)
BASE COUNT           93 a           81 c           48 g          142 t
ORIGIN      
        1 tcatgttgtt tggtccaaat gtaaagaagg cgatgctgca gctaattagc ccgacttgct
       61 accataataa actttcatct ctctctctct ctctctctct ctctctctct ctctctctca
      121 agaatttcag catttctctc cagtttccat attgcctttt aagttctaag aattgtgttg
      181 aaagtatcat ttcttgttca tcttgtaaga gagcacttct catggatatg ttcaataaga
      241 atctcaaagt ttatttaatt caagcatttc ctcttcctct acaagtttat gttcaactta
      301 tcatgatgac catgataatc tttttgcaat atttcgctat attaatttgt gcaactgcag
      361 caga
//