LOCUS BU661702 660 bp mRNA linear EST 06-JAN-2011 DEFINITION cl75f09.z1 Hembase; Erythroid Precursor Cells (LCB:cl library) Homo sapiens cDNA clone cl75f09 5', mRNA sequence. ACCESSION BU661702 VERSION BU661702.1 DBLINK BioSample: SAMN00171628 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 660) AUTHORS Gubin,A.N., Lee,Y.T., Bouffard,G.G. and Miller,J.L. TITLE Gene Expression in Human Erythroid Precursor Cells JOURNAL Unpublished COMMENT Contact: Jeffery L. Miller Laboratory of Chemical Biology National Institute of Diabetes and Digestive and Kidney Diseases Building 10, Room 9B17, National Institutes of Health, Bethesda, MD 20892, USA Tel: 301 402 2373 Fax: 301 435 5148 Email: jm7f@nih.gov The 'cl' library was constructed by Alexander Gubin, Ph.D. in the Laboratory of Chemical Biology, NIDDK, NIH. DNA Sequencing and/or analyses by National Institutes of Health Intramural Sequencing Center (NISC). More information available at: http://hembase.niddk.nih.gov Plate: 75 row: f column: 09 Seq primer: 5' lambda-TriplEx2 Sequencing Primer. FEATURES Location/Qualifiers source 1..660 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="cl75f09" /sex="unknown" /cell_line="Primary Culture of Peripheral Blood Mononuclear Cells" /cell_type="Erythroid Precursor Cells" /tissue_type="blood" /clone_lib="SAMN00171628 Hembase; Erythroid Precursor Cells (LCB:cl library)" /dev_stage="Precursor erythroblasts; GPA++" /lab_host="DH5alpha" /note="Organ: blood; Vector: pTriplEx2; Site_1: SfiI; Site_2: SfiI; A complementary DNA (cDNA) library from human erythroid precursor cells was constructed using SMART PCR (polymerase chain reaction) cDNA Library Construction Kit (Clontech, Palo Alto, CA) according to the manufacturer's directions, but with slight modifications. Briefly, reverse transcription was performed in the presence of 1 umol/L peptide nucleic acid (PNA) oligos (N-terminal)-biotin-GTC-CAC-CCG-AAG-CTT-G-(C-terminal) and (N-terminal)-biotin-C(T/C)T-GAA-GTT-CTC-AGG-A-(C- terminal). Synthesized cDNA was digested with SfiI and size-selected on a 1% agarose gel (>800bp). Large-scale sequencing of the library was performed by the NIH Intramural Sequencing Center (NISC; Http://www.nisc.nih.gov/)." BASE COUNT 199 a 128 c 158 g 174 t ORIGIN 1 cgggttcctc ccgggtttct gccgggtttc tccctgcggc tcctgggttg ttgagactct 61 tgtgaagatg gcttgcgctg ccgcgcggtc cccggccgac caggacaggc tgttgaaaat 121 cctacagcta cagagattca agatgtatgt tcagcagttg gacttaacgt atttcttgag 181 aaaaataaaa tgtactctag agaatggaat cgtgatgtcc aatacagagg cagagtccgg 241 gtccagctca aacaggaaga tgggagcctc tgccttgtac agttcccatc acgtaagtca 301 gtaatgttgt atgcagcaga aatgatacct aaactaaaaa caaggacaca aaaaacagga 361 ggtgctgacc aaagtcttca acaaggagag ggaagtaaaa aagggaaagg aaagaaaaag 421 aagtaaccta gtatcagcat caagtatgtg gtactactgt aagagacatg aatggagact 481 tctaatttgt atcggaggga aacagaagct ttttgtttgc atcatttaac tgaactgtga 541 acccttgtgc ctctcatctt tatcatcgga gttgacagtg aaacaaattt acatcanaag 601 tttgcatctc gcgtatatgc cgtataaaag aatttttttg tctttcaatg cagttttttg //