LOCUS       BT006969                1671 bp    mRNA    linear   HUM 13-MAY-2003
DEFINITION  Homo sapiens t-complex 1 mRNA, complete cds.
ACCESSION   BT006969
VERSION     BT006969.1
KEYWORDS    FLI_CDNA.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
            Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
            Phelan,M. and Farmer,A.
  TITLE     Cloning of human full-length CDSs in BD Creator(TM) System Donor
            vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
            Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
            Phelan,M. and Farmer,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAY-2003) BD Biosciences Clontech, 1020 East Meadow
            Circle, Palo Alto, CA 94303, USA
COMMENT     This CDS clone is a part of a collection of human full length
            expression clones generated by BD Biosciences Clontech and the
            Harvard Institute of Proteomics. Each CDS has been cloned in two
            forms: with and without stop-codon (to allow fusion with C-terminal
            tag). The CDS has been  directionally cloned using BD In-Fusion(TM)
            cloning system between the SalI  and HindIII sites of the pDNR-DUAL
            vector. Additional sequences in the clone:  'ACC' after SalI site
            and before 'ATG' to provide Kozak consensus sequence; 'GG' after
            last codon and before HindIII site to maintain reading frame.
            Clone distribution: http://bioinfo.clontech.com/orfclones.
FEATURES             Location/Qualifiers
     source          1..1671
                     /db_xref="H-InvDB:HIT000099889"
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="GH00379X1.0"
                     /clone_lib="BD Creator(TM) CDS Library derived from MGC
                     collection"
                     /lab_host="DH5alpha T1 resistant"
                     /note="Vector: pDNR-Dual"
     CDS             1..1671
                     /codon_start=1
                     /product="t-complex 1"
                     /protein_id="AAP35615.1"
                     /translation="MEGPLSVFGDRSTGETIRSQNVMAAASIANIVKSSLGPVGLDKM
                     LVDDIGDVTITNDGATILKLLEVEHPAAKVLCELADLQDKEVGDGTTSVVIIAAELLK
                     NADELVKQKIHPTSVISGYRLACKEAVRYINENLIVNTDELGRDCLINAAKTSMSSKI
                     IGINGDFFANMVVDAVLAIKYTDIRGQPRYPVNSVNILKAHGRSQMESMLISGYALNC
                     VVGSQGMPKRIVNAKIACLDFSLQKTKMKLGVQVVITDPEKLDQIRQRESDITKERIQ
                     KILATGANVILTTGGIDDMCLKYFVEAGAMAVRRVLKRDLKRIAKASGATILSTLANL
                     EGEETFEAAMLGQAEEVVQERICDDELILIKNTKARTSASIILRGANDFMCDEMERSL
                     HDALCVVKRVLESKSVVPGGGAVEAALSIYLENYATSMGSREQLAIAEFARSLLVIPN
                     TLAVNAAQDSTDLVAKLRAFHNEAQVNPERKNLKWIGLDLSNGKPRDNKQAGVFEPTI
                     VKVKSLKFATEAAITILRIDDLIKLHPESKDDKHGSYEDAVHSGALND"
BASE COUNT          517 a          303 c          399 g          452 t
ORIGIN      
        1 atggaggggc ctttgtccgt gttcggtgac cgcagcactg gggaaacgat ccgctcccaa
       61 aacgttatgg ctgcagcttc gattgccaat attgtaaaaa gttctcttgg tccagttggc
      121 ttggataaaa tgttggtgga tgatattggt gatgtaacca ttactaacga tggtgcaacc
      181 atcctgaagt tactggaggt agaacatcct gcagctaaag ttctttgtga gctggctgat
      241 ctgcaagaca aagaagttgg agatggaact acttcagtgg ttattattgc agcagaactc
      301 ctaaaaaatg cagatgaatt agtcaaacag aaaattcatc ccacatcagt tattagtggc
      361 tatcgacttg cttgcaagga agcagtgcgt tatatcaatg aaaacctaat tgttaacaca
      421 gatgaactgg gaagagattg cctgattaat gctgctaaga catccatgtc ttccaaaatc
      481 attggaataa atggtgattt ctttgctaac atggtagtag atgctgtact tgctattaaa
      541 tacacagaca taagaggcca gccacgctat ccagtcaact ctgttaatat tttgaaagcc
      601 catgggagaa gtcaaatgga gagtatgctc atcagtggct atgcactcaa ctgtgtggtg
      661 ggatcccagg gcatgcccaa gagaatcgta aatgcaaaaa ttgcttgcct tgacttcagc
      721 ctgcaaaaaa caaaaatgaa gcttggtgta caggtggtca ttacagaccc tgaaaaactg
      781 gaccaaatta gacagagaga atcagatatc accaaggaga gaattcagaa gatcctggca
      841 actggtgcca atgttattct aaccactggt ggaattgatg atatgtgtct gaagtatttt
      901 gtggaggctg gtgctatggc agttagaaga gttttaaaaa gggaccttaa acgcattgcc
      961 aaagcttctg gagcaactat tctgtcaacc ctggccaatt tggaaggtga agaaactttt
     1021 gaagctgcaa tgttgggaca ggcagaagaa gtggtacagg agagaatttg tgatgatgag
     1081 ctgatcttaa tcaaaaatac taaggctcgt acgtctgcat cgattatctt acgtggggca
     1141 aatgatttca tgtgtgatga gatggagcgc tctttacatg atgcactttg tgtagtgaag
     1201 agagttttgg agtcaaaatc tgtggttccc ggtgggggtg ctgtagaagc agccctttcc
     1261 atataccttg aaaactatgc aaccagcatg gggtctcggg aacagcttgc gattgcagag
     1321 tttgcaagat cacttcttgt tattcccaat acactagcag ttaatgctgc ccaggactcc
     1381 acagatctgg ttgcaaaatt aagagctttt cataatgagg cccaggttaa cccagaacgt
     1441 aaaaatctaa aatggattgg tcttgatttg agcaatggta aacctcgaga caacaaacaa
     1501 gcaggggtgt ttgaaccaac catagttaaa gttaagagtt tgaaatttgc aacagaagct
     1561 gcaatcacca ttcttcgaat tgatgatctt attaaattac atccagaaag taaagatgat
     1621 aaacatggaa gttatgaaga tgctgttcac tctggagccc ttaatgatta g
//