LOCUS       BM202328                 491 bp    mRNA    linear   EST 16-DEC-2010
DEFINITION  C0237F08-3 NIA Mouse 7.5-dpc Whole Embryo cDNA Library (Long) Mus
            musculus cDNA clone NIA:C0237F08 IMAGE:30014179 3', mRNA sequence.
ACCESSION   BM202328
VERSION     BM202328.2
DBLINK      BioSample: SAMN00169965
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.H.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res. 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     On Dec 14, 2001 this sequence version replaced BM202328.1.
            Other_ESTs: C0237F08-5N
            Contact: Dawood B. Dudekula
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            Plate: C0237  row: F  column: 08
            Seq primer: -21M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:C0237F08-3"
                     /db_xref="taxon:10090"
                     /clone="NIA:C0237F08 IMAGE:30014179"
                     /tissue_type="whole embryo including extraembryonic
                     tissues at 7.5-days postcoitum"
                     /clone_lib="SAMN00169965 NIA Mouse 7.5-dpc Whole Embryo
                     cDNA Library (Long)"
                     /dev_stage="7.5-days postcoitum"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1 (Invitrogen); Site_1: SalI; Site_2:
                     NotI; Mouse cDNA project by the Laboratory of Genetics,
                     National Institute on Aging (NIA), Intramural Research
                     Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is
                     a long-transcript enriched cDNA library (Ref. Genome Res.
                     11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were
                     extracted from a pool of four embryos at 7.5-days
                     postcoitum. Double-stranded cDNAs were synthesized with an
                     Oligo(dT) primer [Invitrogen:
                     5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from
                     7 ug of total RNA, treated with T4 DNA polymerase, and
                     purified by ethanol-precipitation. The cDNAs were ligated
                     to Lone-linker LL-Sal4, purified by phenol/chloroform, and
                     separated from free linkers by Centricon 100. Then, the
                     cDNAs were amplified by long-range high fidelity PCR using
                     Ex Taq polymerase (Takara) with a primer Sal4-S. The
                     products were purified by phenol/chloroform and Centricon
                     100. The cDNAs were digested with SalI and NotI enzymes
                     and cloned into SalI/NotI site of pSPORT1 plasmid vector.
                     The DH10B E. coli host was transformed with the ligation
                     mixture by the standard chemical method. The average
                     insert size is about 2.2 kb. The library was constructed
                     by Yulan Piao (NIA)."
BASE COUNT          191 a           81 c           71 g          148 t
ORIGIN      
        1 agaaaaagga aagctgattt ttattttatc aacagccatt cttaaacatg aacatgcata
       61 cgtaatattc tacgcacaca tcacagtttt taacgttagt taataatgtt aaacacacaa
      121 catacaacct taaaaaaaaa gtcaacctgc aaacttaaaa ctttaaacaa agtcttacta
      181 atttaaaaaa ggttttgtgt tgggtaccat ttgtacaaca cagttaattt aaacatttcc
      241 attttggctg cacatgaaaa agcggcagta gaaaataaat ttatgagggt tttaaatagc
      301 agaataggca gtttcgccat gcaggagaag caatattaaa tattagtttt caaaaaaaat
      361 ccacatttaa aaatatttag ttcaagtcac agaacttttc tcagtagaga cctcaatgca
      421 atgcgtaatt agctgcctta gatggcctgt tttgaaagga caaaatccct ttcaaagtat
      481 aactttactg a
//