LOCUS       BE637430                 453 bp    mRNA    linear   EST 25-AUG-2000
DEFINITION  WHE1817-1820_B05_B05ZS Secale cereale anther cDNA library Secale
            cereale cDNA clone WHE1817-1820_B05_B05, mRNA sequence.
ACCESSION   BE637430
VERSION     BE637430.1
DBLINK      BioSample: SAMN00160054
KEYWORDS    EST.
SOURCE      Secale cereale (rye)
  ORGANISM  Secale cereale
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP
            clade; Pooideae; Triticodae; Triticeae; Hordeinae; Secale.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Anderson,O.D., Butler,E., Chao,S., Choi,D.W., Close,T.J.,
            Fenton,R.D., Gustafson,J.P., Han,P.S., Hsia,C.C., Kang,Y.,
            Lazo,G.R., Miller,R., Rausch,C.J., Ross,K., Seaton,C.L. and
            Tong,J.C.
  TITLE     The structure and function of the expressed portion of the wheat
            genomes - Anther cDNA library from rye
  JOURNAL   Unpublished
COMMENT     Contact: Olin Anderson
            US Department of Agriculture, Agriculture Research Service, Pacific
            West Area, Western Regional Research Center
            800 Buchanan Street, Albany, CA 94710, USA
            Tel: 5105595773
            Fax: 5105595818
            Email: oandersn@pw.usda.gov
            Sequence have been trimmed to remove vector sequence and low
            quality sequence with phred score less than 20
            Seq primer: Stratagene SK primer.
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Secale cereale"
                     /mol_type="mRNA"
                     /cultivar="Blanco"
                     /db_xref="taxon:4550"
                     /clone="WHE1817-1820_B05_B05"
                     /tissue_type="Anther"
                     /clone_lib="SAMN00160054 Secale cereale anther cDNA
                     library"
                     /dev_stage="Adult plant before anthesis"
                     /lab_host="E. coli SOLR"
                     /note="Vector: Lambda Uni-ZAP XR, excised phagemid;
                     Site_1: EcoRI; Site_2: XhoI; Plants were grown in the
                     greenhouse. Anthers were harvested and pooled from early
                     meiosis to late meiosis. The tissue, total RNA, and
                     poly(A) RNA were prepared (Butler, Ross and Gustafson) at
                     University of Missouri, Columbia. A cDNA library was made,
                     and the cDNA clones were in vivo excised to give
                     pBluescript phagemids in the TJ Close lab (Choi, Close,
                     Fenton) at the University of California, Riverside.
                     Plasmid DNA preparations and DNA sequencing were performed
                     in the OD Anderson lab (all other authors)."
BASE COUNT           97 a          142 c          120 g           94 t
ORIGIN      
        1 tgcgacgatc ccgccaccag ggccaagctc gaacgcctct gcaagaccac aacagtgagg
       61 acaaggtaca ctgtcatgtc gaaggagctc ctggatgaac acccagaatt gaagacagaa
      121 ggcactccaa cattgacacc acggcttgac atctgcaatg ccgcggtgct tgaacttggt
      181 gctgctgcag ctcgtgccgc ccttggtgag tggggccgtc cagcagctga tattacccac
      241 ttggtctaca tctcctccag tgagcttcgc ctcccagggg gtgatctttt cctggcaacc
      301 cgtcttggcc tctctccaaa cacagtgcgc acatcccttc tcttccttgg ctgctcaggt
      361 ggagctgccg ccctccgcac tgccaaggac attgcagaga acaacccagg gagccgtgtg
      421 cttgtaataa cggcggagac aactgtgttg ggc
//