LOCUS       BE516326                 549 bp    mRNA    linear   EST 08-AUG-2000
DEFINITION  WHE617_H12_O23ZA Wheat ABA-treated embryo cDNA library Triticum
            aestivum cDNA clone WHE617_H12_O23, mRNA sequence.
ACCESSION   BE516326
VERSION     BE516326.1
DBLINK      BioSample: SAMN00160090
KEYWORDS    EST.
SOURCE      Triticum aestivum (bread wheat)
  ORGANISM  Triticum aestivum
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP
            clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Anderson,O.D., Chao,S., Han,P.S., Hsia,C.C., Johnson,R.R., Kang,Y.,
            Lazo,G.R., Miller,R., Rausch,C.J., Seaton,C.L., Tong,J.C.,
            Verhey,S.D. and Walker-Simmons,M.K.
  TITLE     The structure and function of the expressed portion of the wheat
            genomes - ABA-treated embryo library
  JOURNAL   Unpublished
COMMENT     Contact: Olin Anderson
            US Department of Agriculture, Agriculture Research Service, Pacific
            West Area, Western Regional Research Center
            800 Buchanan Street, Albany, CA 94710, USA
            Tel: 5105595773
            Fax: 5105595818
            Email: oandersn@pw.usda.gov
            Sequence have been trimmed to remove vector sequence and low
            quality sequence with phred score less than 20
            Seq primer: Clontech Matchmaker 3' AD primer.
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Triticum aestivum"
                     /mol_type="mRNA"
                     /cultivar="Brevor (soft, white, winter, common wheat)"
                     /db_xref="taxon:4565"
                     /clone="WHE617_H12_O23"
                     /tissue_type="Seed embryo"
                     /clone_lib="SAMN00160090 Wheat ABA-treated embryo cDNA
                     library"
                     /dev_stage="Mature dormant seeds"
                     /lab_host="E. coli DH12S"
                     /note="Vector: pGAD10; Site_1: EcoRI; Site_2: XhoI;
                     Embryos were cut from mature, dormant seeds and imbibed in
                     25 microM ABA (abscisic acid) in 5 mM Mes buffer, pH 5.7,
                     for 12 hr at 22 C. The tissue, total RNA, and poly(A) RNA
                     were prepared by Steven Verhey in M.K. Walker-Simmons's
                     lab (USDA-ARS, Washington State Univ., Pullman, Washington
                     99164-6420. A cDNA library was made by Clontech using a
                     combination of random and oligo dT primers. Library was
                     plated and archived by Russell Johnson (Colby College,
                     ME/Walker-Simmons' lab). Plasmid DNA preparations and DNA
                     sequencing were performed in the OD Anderson lab (all
                     other authors)."
BASE COUNT          150 a          139 c          126 g          134 t
ORIGIN      
        1 tcgcggccgc gtcgactttt tttttttttt ttttggagac caagatggta atctatttag
       61 tagcaatcct gaatgcacct caaacacacc agatctgaaa tattaagttg acagacaaaa
      121 atgccgaaaa ggggaaggca gagcgttcac aaaaggaatg aaacgagcag ctcagctcag
      181 ctcatacata tgccagtcac taccacatag cgagctcact gcaccatctc catgtccttg
      241 aagtactcat gctcaagagc ctgcctggcc gtgatcctct tgtttggctc gaaccgaagc
      301 attttcgaga gaaggtccag gccaacaggt tcaagattgg ggacaacggt tgcaaggtcc
      361 tctgcctgcc acctggggaa ggcggacttg tagtcaggca aggagctcac gcctggccaa
      421 gtttgttcat ttggagtgcc cagtaccctg aatatcttaa atagttcatc aatctcaaaa
      481 tcgccaggga acagtggttt ctggttcacc atttctgcaa gatacagccc actgaccaca
      541 cgtcaactg
//