LOCUS       BC164240                 958 bp    mRNA    linear   VRT 18-MAR-2009
DEFINITION  Danio rerio APEX nuclease (multifunctional DNA repair enzyme) 1,
            mRNA (cDNA clone MGC:191415 IMAGE:100059724), complete cds.
ACCESSION   BC164240
VERSION     BC164240.1
KEYWORDS    MGC.
SOURCE      Danio rerio (zebrafish)
  ORGANISM  Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Danionidae; Danioninae; Danio.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
            Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
            Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
            Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
            Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
            Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
            Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
            Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
            Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
            McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
            Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
            Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
            Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
            Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
            Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
            Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
            Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
            Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
  CONSRTM   Mammalian Gene Collection Program Team
  TITLE     Generation and initial analysis of more than 15,000 full-length
            human and mouse cDNA sequences
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
   PUBMED   12477932
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NIH MGC Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2008) National Institutes of Health, Mammalian
            Gene Collection (MGC), Bethesda, MD 20892-2590, USA
  REMARK    NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT     Contact: MGC help desk
            Email: cgapbs-r@mail.nih.gov
            Tissue Procurement: Genecopoeia
            cDNA Library Preparation: GeneCopoeia, Inc
            cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
            DNA Sequencing by: (null)
            
            Clone distribution: MGC clone distribution information can be found
            through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
            Series: OCAF Plate: 4 Row: b Column: 1.
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Danio rerio"
                     /mol_type="mRNA"
                     /db_xref="taxon:7955"
                     /clone="MGC:191415 IMAGE:100059724"
                     /tissue_type="PCR rescue, ZGC template clones."
                     /clone_lib="NIH_ZGC_50"
                     /lab_host="DH10B"
                     /note="Vector: pENTR221, with TAG stop codon"
     gene            1..958
                     /gene="apex1"
                     /gene_synonym="hm:zehn1532"
                     /gene_synonym="wu:fc13d11"
                     /gene_synonym="zgc:66204"
                     /db_xref="GeneID:406730"
                     /db_xref="ZFIN:ZDB-GENE-040426-2761"
     CDS             35..925
                     /gene="apex1"
                     /gene_synonym="hm:zehn1532"
                     /gene_synonym="wu:fc13d11"
                     /gene_synonym="zgc:66204"
                     /codon_start=1
                     /product="apex1 protein"
                     /protein_id="AAI64240.1"
                     /db_xref="GeneID:406730"
                     /db_xref="ZFIN:ZDB-GENE-040426-2761"
                     /translation="MPKRAKKNEEGVDGEADNGTAAAKKEKKGKEPEAPILYEDPPEK
                     LTSKDGRAANMKITSWNVDGLRAWVKKNGLDWVRKEKALPADITGMPEYPHKYWAGSE
                     DKEGYSGVAMLCKTEPLNVTYGIGKEEHDKEGRVITAEFPDFFLVTAYVPNASRGLVR
                     LDYRKTWDVDFRAYLCGLDARKPLVLCGDLNVAHQEIDLKNPKGNRKNAGFTPEEREG
                     FTQLLEAGFTDSFRELYPDQAYAYTFWTYMMNARSKNVGWRLDYFVLSSALLPGLCDS
                     KIRNTAMGSDHCPITLFLAV"
BASE COUNT          244 a          234 c          284 g          196 t
ORIGIN      
        1 gtacaaaaaa gcaggcttga aggaattcgg taccatgccc aaaagagcca agaagaacga
       61 ggaaggtgtt gatggagagg ctgacaatgg aactgcagct gcaaagaaag agaaaaaggg
      121 gaaggagccc gaggccccca ttctgtatga agatcctcca gaaaagctga ccagtaagga
      181 cggccgggca gccaatatga agatcacctc ctggaatgtg gacggcctgc gggcgtgggt
      241 caaaaagaac ggacttgatt gggtgcggaa ggagaaagct ctgccggcgg acatcactgg
      301 catgcctgag tatccacaca aatactgggc aggatcagag gataaggagg gctacagtgg
      361 agtggccatg ctctgcaaga ccgaaccact caatgtcacc tatggcattg gtaaagagga
      421 gcacgataaa gagggccggg tcatcactgc cgagtttcct gatttcttcc tggtcacggc
      481 gtacgtgcct aatgccagtc gtggtttagt gcggcttgac taccgcaaga cttgggatgt
      541 ggatttccga gcctacctgt gcggtttaga cgcgcgcaaa cccctggtgc tgtgtggaga
      601 tctgaacgtg gcccaccagg agatcgatct gaaaaacccc aagggaaacc gcaagaacgc
      661 gggcttcacc cctgaggaac gggagggctt cacacagctc ctggaggccg gctttactga
      721 tagttttcgg gagctctatc cagatcaggc ctacgcctac accttctgga cctacatgat
      781 gaacgctcgc tccaaaaacg tgggttggcg tttggactac tttgtgctgt cgtctgcttt
      841 gctgccgggt ttgtgcgaca gcaagatccg aaacacagcc atgggaagtg atcactgtcc
      901 tattaccttg tttttggcag tgtagctcga gtgcggccgc aacccagctt tcttgtac
//