LOCUS       BC122610                1382 bp    mRNA    linear   MAM 23-JAN-2007
DEFINITION  Bos taurus APEX nuclease (multifunctional DNA repair enzyme) 1,
            mRNA (cDNA clone MGC:139790 IMAGE:8282655), complete cds.
ACCESSION   BC122610
VERSION     BC122610.1
KEYWORDS    MGC.
SOURCE      Bos taurus (domestic cattle)
  ORGANISM  Bos taurus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Laurasiatheria; Artiodactyla; Ruminantia;
            Pecora; Bovidae; Bovinae; Bos.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Moore,S., Alexander,L., Brownstein,M., Guan,L., Lobo,S., Meng,Y.,
            Tanaguchi,M., Wang,Z., Yu,J., Prange,C., Schreiber,K., Shenmen,C.,
            Wagner,L., Bala,M., Barbazuk,S., Barber,S., Babakaiff,R.,
            Beland,J., Chun,E., Del Rio,L., Gibson,S., Hanson,R.,
            Kirkpatrick,R., Liu,J., Matsuo,C., Mayo,M., Santos,R.R., Stott,J.,
            Tsai,M., Wong,D., Siddiqui,A., Holt,R., Jones,S.J. and Marra,M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2006) BC Cancer Agency, Canada's Michael Smith
            Genome Sciences Centre, Suite 100, 570 West 7th Avenue, Vancouver,
            British Columbia V5Z 4S6, Canada
  REMARK    NIH-MGC Project
COMMENT     Contact: Robert Kirkpatrick
            Canada's Michael Smith Genome Sciences Centre
            BC Cancer Agency
            Suite 100, 570 West 7th Avenue, Vancouver, British Columbia,
            Canada, V5Z 4S6
            Tel: 1-604-707-5900 x5406
            Fax: 1-604-876-3561
            Email: robertk@bcgsc.ca
            Tissue Procurement: Lee Alexander, USDA, ARS, NPA
            cDNA Library Preparation: Express Genomics
            cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
            DNA Sequencing by: Bovine Genome Sequencing Program, Genome
            Sequence Centre,
            BC Cancer Agency, Vancouver, BC, Canada
            info@bcgsc.bc.ca
            Moore S, Alexander L, Brownstein M, Guan L, Lobo S, Meng Y,
            Taniguchi M, Wang Z, Prange C, Schreiber K, Shenmen C, Wagner L,
            Ali J, Bala M, Barber S, Babakaiff R, Beland J, Chand S, Chun E,
            Del Rio L, Gibson S, Kirkpatrick R, Liu J, Matsuo C, Mayo M,
            Palmquist D, Santos RR, Stott J, Tsai M, Wong D, Wynhoven B, Hanson
            R, Siddiqui A, Holt R, Jones SJ, Marra MA
            
            Clone distribution: MGC clone distribution information can be found
            through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
            Series: IRAK Plate: 269 Row: e Column: 3.
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Bos taurus"
                     /mol_type="mRNA"
                     /strain="L1 Hereford"
                     /db_xref="taxon:9913"
                     /clone="MGC:139790 IMAGE:8282655"
                     /tissue_type="Basal ganglia, female calf 8.5 months, L1
                     Hereford"
                     /clone_lib="GC_BGC-27"
                     /lab_host="DH10B"
                     /note="Vector: pExpress1"
     gene            1..1382
                     /gene="APEX1"
                     /gene_synonym="APEN"
                     /gene_synonym="BAP1"
                     /db_xref="GeneID:281630"
     CDS             115..1071
                     /gene="APEX1"
                     /gene_synonym="APEN"
                     /gene_synonym="BAP1"
                     /codon_start=1
                     /product="APEX nuclease (multifunctional DNA repair
                     enzyme) 1"
                     /protein_id="AAI22611.1"
                     /db_xref="GeneID:281630"
                     /translation="MPKRGKKGAVVEDAEEPKTEPEAKKSKAGAKKNEKEAVGEGAVL
                     YEDPPDQKTSPSGKSATLKICSWNVDGLRAWIKKKGLDWVKEEAPDILCLQETKCSEN
                     KLPVELQELSGLSHQYWSAPSDKEGYSGVGLLSRQCPLKVSYGIGEEEHDQEGRVIVA
                     EYDAFVLVTAYVPNAGRGLVRLEYRQRWDEAFRKFLKGLASRKPLVLCGDLNVAHEEI
                     DLRNPKGNKKNAGFTPQERQGFGELLQAVPLTDSFRHLYPNTAYAYTFWTYMMNARSK
                     NVGWRLDYFLLSQSLLPALCDSKIRSKALGSDHCPITLYLAL"
BASE COUNT          398 a          311 c          346 g          327 t
ORIGIN      
        1 gggttaagga ggaggcatac aggggccgca gcaggtcagc taaagggcag cgcgatcaaa
       61 atactgcttc agtgggcgaa gtggaactgg tgcagaaggg catacattac agtgatgccg
      121 aaacgtggga aaaagggagc ggtggtcgaa gacgcggaag agcccaagac tgagccagag
      181 gcgaagaaga gtaaggcagg agcgaaaaag aacgaaaaag aggcagtagg agagggcgca
      241 gttctgtatg aagacccccc agatcagaaa acctcaccca gtggcaaatc agccacactc
      301 aagatctgct cgtggaatgt ggatgggctt cgagcctgga ttaagaagaa aggtttagat
      361 tgggtaaagg aagaagcccc agacatcctg tgcctccaag agaccaaatg ttcagagaac
      421 aaactaccag ttgaacttca agaactgtct ggattgtccc atcagtactg gtcagctcct
      481 tcagacaagg aagggtacag tggtgtgggc ctcctctccc gccagtgccc actcaaagtc
      541 tcttatggca ttggtgagga agaacatgat caggaaggcc gagtgattgt ggctgaatat
      601 gacgcatttg tgctggtgac agcctatgtg cctaatgcag gccgaggtct ggtgcgcctg
      661 gagtatcgcc agcgctggga tgaagctttt cgcaaattcc tgaagggttt ggcatcccgc
      721 aagccccttg tgctatgtgg ggacctcaat gtggctcatg aagaaattga ccttcgcaac
      781 ccgaagggaa ataaaaagaa tgctggcttc actccacaag agcggcaggg cttcggggaa
      841 ttgctgcagg ctgtgccact cactgacagt ttccggcatc tctaccccaa caccgcctat
      901 gcctacacct tctggaccta tatgatgaat gcgcgatcca aaaacgttgg ttggcgcctt
      961 gattattttt tgttatctca gtctctgttg cctgcattgt gtgacagtaa aatccgttcc
     1021 aaggctctgg gcagtgacca ctgtcccatt accctatacc tagctctgtg acacctcttc
     1081 caaatcacat tgagcctggg aaataagccc accaagctag ctttaaaatt cttaccccca
     1141 aacttcttta ataattgcta tctagagaca tctgcattgt atttcccttc taaactatga
     1201 atcctttaac caggtttcta ataacagatg taagttctca agaagggtgt ttgtgtaggg
     1261 gtagtgcttt tttctttttc tttttttaca ttaatcaaaa gctactgata cttcctttga
     1321 actgtccctg tgaaaataaa gagccatagt ttcaaaaaaa aaaaaaaaaa aaaaaaaaaa
     1381 aa
//