LOCUS       BB520689                 319 bp    mRNA    linear   EST 08-SEP-2005
DEFINITION  Mus musculus 16 days neonate heart cDNA, RIKEN full-length
            enriched library, clone:D830045I23, 3' end partial sequence,
            similar to ddbj:U67321 Mus musculus Lice2 cysteine protease
            (Lice2) mRNA.
ACCESSION   BB520689
VERSION     BB520689.1
KEYWORDS    EST; 3'-end sequence (3'-EST).
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Konno,H., Aizawa,K., Akahira,S., Akiyama,J., Arakawa,T.,
            Carninci,P., Endo,T., Fukuda,S., Fukunishi,Y., Hara,A.,
            Hayatsu,N., Hirozane,T., Hori,F., Ishii,Y., Ishikawa,J.,
            Ishikawa,T., Itoh,M., Izawa,M., Kadota,K., Kagawa,I., Kai,C.,
            Kawai,J., Kikuchi,N., Kiyosawa,H., Kojima,Y., Kondo,S., Koya,S.,
            Kurihara,C., Kusakabe,M., Matsuyama,T., Miki,R., Mizuno,Y.,
            Nakamura,M., Oda,H., Okazaki,Y., Ono,T., Owa,C., Saito,H.,
            Sakai,C., Sato,K., Shibata,K., Shibata,Y., Shigemoto,Y.,
            Shinagawa,A., Shiraki,T., Sogabe,Y., Sugahara,Y., Suzuki,H.,
            Suzuki,H., Tagawa,A., Takahashi,F., Tominaga,N., Toya,T.,
            Tsunoda,Y., Watahiki,A., Watanabe,S., Yamamura,T., Yamanaka,I.,
            Yano,R., Yasunishi,A., Yokota,T., Yoshida,K., Yoshiki,A.,
            Yoshino,M., Muramatsu,M. and Hayashizaki,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUN-2000)
            Contact:Yoshihide Hayashizaki
            The Institute of Physical and Chemical Research (RIKEN), Omics
            Science Center, RIKEN Yokohama Institute; 1-7-22 Suehiro-cho,
            Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
            URL    :http://www.osc.riken.jp/
REFERENCE   2
  AUTHORS   
  CONSRTM   The FANTOM Consortium, Riken Genome Exploration Research Group and
            Genome Science Group (Genome Network Project Core Group)
  TITLE     The Transcriptional Landscape of the Mammalian Genome
  JOURNAL   Science 309, 1559-1563 (2005)
REFERENCE   3
  AUTHORS   
  CONSRTM   RIKEN Genome Exploration Research Group and Genome Science Group
            (Genome Network Project Core Group) and the FANTOM Consortium
  TITLE     Antisense Transcription in the Mammalian Transcriptome
  JOURNAL   Science 309, 1564-1566 (2005)
REFERENCE   4
  AUTHORS   Carninci,P. and Hayashizaki,Y.
  TITLE     High-efficiency full-length cDNA cloning
  JOURNAL   Meth. Enzymol. 303, 19-44 (1999)
REFERENCE   5
  AUTHORS   Carninci,P., Nishiyama,Y., Westover,A., Itoh,M., Nagaoka,S.,
            Sasaki,N., Okazaki,Y., Muramatsu,M. and Hayashizaki,Y.
  TITLE     Thermostabilization and thermoactivation of thermolabile enzymes
            by trehalose and its application for the synthesis of full length
            cDNA
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 95 (2), 520-524 (1998)
REFERENCE   6
  AUTHORS   Itoh,M., Kitsunai,T., Akiyama,J., Shibata,K., Izawa,M., Kawai,J.,
            Tomaru,Y., Carninci,P., Shibata,Y., Ozawa,Y., Muramatsu,M.,
            Okazaki,Y. and Hayashizaki,Y.
  TITLE     Automated filtration-based high-throughput plasmid preparation
            system
  JOURNAL   Genome Res. 9 (5), 463-470 (1999)
COMMENT     Please visit our web site (http://www.osc.riken.jp/) for further
            details.
            cDNA library was prepared and sequenced in Mouse Genome
            Encyclopedia Project of Genome Exploration Research Group in Riken
            Genomic Sciences Center and Genome Science Laboratory in RIKEN.
            Division of Experimental Animal Research in Riken contributed to
            prepare mouse tissues. 1st strand cDNA was primed with a primer
            [5' GAGAGAGAGAGCGGCCGCAACTCGAGTTTTTTTTTTTTTTTTVN 3'], cDNA was
            prepared by using trehalose thermo-activated reverse transcriptase
            and subsequently enriched for full-length by cap-trapper. Second
            strand cDNA was prepared with the primer adapter of sequence [5'
            GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was
            cleaved with BamHI and XhoI. Vector: a modified pBluescript KS(+)
            after bulk excision from Lambda FLC I. Cloning sites, 5' end:
            SalI; 3' end: BamHI. Host: DH10B.
FEATURES             Location/Qualifiers
     source          1..319
                     /clone="D830045I23"
                     /db_xref="taxon:10090"
                     /dev_stage="16 days neonate"
                     /mol_type="mRNA"
                     /note="clone_lib: RIKEN full-length enriched mouse cDNA
                     library"
                     /organism="Mus musculus"
                     /tissue_type="heart"
BASE COUNT           77 a           75 c           41 g          126 t
ORIGIN      
        1 gcatcctcct tttccatata attctgatga ttctaaattt caacctctct tacacacctt
       61 ttcattcatt tgacatctcc agatttgatc gatctacttt aagatttcta ggtgggagtt
      121 tcttactctt gtttgaacta tgctgaagag cgaaagtgag tgattacttt gaccccttcc
      181 tggctctttt ccaccttcca attctacacc cattcttctc gttttattca ttgtatgtaa
      241 attcctttta tttttttttt aatgtgccgt acacatcacc ctcactgagc taataaaggg
      301 aaaagaatgt ttcacatcc
//