LOCUS       BB072002                 241 bp    mRNA    linear   EST 07-SEP-2005
DEFINITION  Mus musculus adult male colon cDNA, RIKEN full-length enriched
            library, clone:9030002P03, 3' end partial sequence, similar to
            ddbj:AK000109 Homo sapiens cDNA FLJ20102 fis, clone COL04783.
ACCESSION   BB072002
VERSION     BB072002.1
KEYWORDS    EST; 3'-end sequence (3'-EST).
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Konno,H., Aizawa,K., Akahira,S., Akiyama,J., Arakawa,T.,
            Carninci,P., Endo,T., Fukuda,S., Fukunishi,Y., Hara,A.,
            Hayatsu,N., Hirozane,T., Hori,F., Ishii,Y., Ishikawa,J.,
            Ishikawa,T., Itoh,M., Izawa,M., Kadota,K., Kagawa,I., Kai,C.,
            Kawai,J., Kikuchi,N., Kiyosawa,H., Kojima,Y., Kondo,S., Koya,S.,
            Kurihara,C., Kusakabe,M., Matsuyama,T., Miki,R., Mizuno,Y.,
            Nakamura,M., Oda,H., Okazaki,Y., Ono,T., Owa,C., Saito,H.,
            Sakai,C., Sato,K., Shibata,K., Shibata,Y., Shigemoto,Y.,
            Shinagawa,A., Shiraki,T., Sogabe,Y., Sugahara,Y., Suzuki,H.,
            Suzuki,H., Tagawa,A., Takahashi,F., Tominaga,N., Toya,T.,
            Tsunoda,Y., Watahiki,A., Watanabe,S., Yamamura,T., Yamanaka,I.,
            Yano,R., Yasunishi,A., Yokota,T., Yoshida,K., Yoshiki,A.,
            Yoshino,M., Muramatsu,M. and Hayashizaki,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2000)
            Contact:Yoshihide Hayashizaki
            The Institute of Physical and Chemical Research (RIKEN), Omics
            Science Center, RIKEN Yokohama Institute; 1-7-22 Suehiro-cho,
            Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
            URL    :http://www.osc.riken.jp/
REFERENCE   2
  AUTHORS   
  CONSRTM   The FANTOM Consortium, Riken Genome Exploration Research Group and
            Genome Science Group (Genome Network Project Core Group)
  TITLE     The Transcriptional Landscape of the Mammalian Genome
  JOURNAL   Science 309, 1559-1563 (2005)
REFERENCE   3
  AUTHORS   
  CONSRTM   RIKEN Genome Exploration Research Group and Genome Science Group
            (Genome Network Project Core Group) and the FANTOM Consortium
  TITLE     Antisense Transcription in the Mammalian Transcriptome
  JOURNAL   Science 309, 1564-1566 (2005)
REFERENCE   4
  AUTHORS   Carninci,P. and Hayashizaki,Y.
  TITLE     High-efficiency full-length cDNA cloning
  JOURNAL   Meth. Enzymol. 303, 19-44 (1999)
REFERENCE   5
  AUTHORS   Carninci,P., Nishiyama,Y., Westover,A., Itoh,M., Nagaoka,S.,
            Sasaki,N., Okazaki,Y., Muramatsu,M. and Hayashizaki,Y.
  TITLE     Thermostabilization and thermoactivation of thermolabile enzymes
            by trehalose and its application for the synthesis of full length
            cDNA
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 95 (2), 520-524 (1998)
REFERENCE   6
  AUTHORS   Itoh,M., Kitsunai,T., Akiyama,J., Shibata,K., Izawa,M., Kawai,J.,
            Tomaru,Y., Carninci,P., Shibata,Y., Ozawa,Y., Muramatsu,M.,
            Okazaki,Y. and Hayashizaki,Y.
  TITLE     Automated filtration-based high-throughput plasmid preparation
            system
  JOURNAL   Genome Res. 9 (5), 463-470 (1999)
COMMENT     Please visit our web site (http://www.osc.riken.jp/) for further
            details.
            cDNA library was prepared and sequenced in Mouse Genome
            Encyclopedia Project of Genome Exploration Research Group in Riken
            Genomic Sciences Center and Genome Science Laboratory in RIKEN.
            Division of Experimental Animal Research in Riken contributed to
            prepare mouse tissues. 1st strand cDNA was primed with a primer
            [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was
            prepared by using trehalose thermo-activated reverse transcriptase
            and subsequently enriched for full-length by cap-trapper. cDNA
            went through one round of normalization to Rot = 10.0 and
            subtraction to Rot = 185.0. Second strand cDNA was prepared with
            the primer adapter of sequence [5'
            GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was
            cleaved with XhoI and BamHI. Vector: a modified pBluescript KS(+)
            after bulk excision from Lambda FLC I. Cloning sites, 5' end:
            SalI; 3' end: BamHI. Host: DH10B.
FEATURES             Location/Qualifiers
     source          1..241
                     /clone="9030002P03"
                     /db_xref="taxon:10090"
                     /dev_stage="adult"
                     /mol_type="mRNA"
                     /note="clone_lib: RIKEN full-length enriched mouse cDNA
                     library"
                     /organism="Mus musculus"
                     /sex="male"
                     /tissue_type="colon"
BASE COUNT           61 a           52 c           38 g           90 t
ORIGIN      
        1 cgtgatgacc gattcgcaat tgcgaatcag cttttactca cttggacatc cctgctcttg
       61 cattttgctg cccctcttcc atgtgctccc aattccagct actggaagcc accagggatg
      121 ctttctaatt atcatttgca actagaactg tattcagaaa gaaaatttgt atttttgtat
      181 aaattgattg tgtgccattt tatataacag ttcctgtttt acaaataaat tttgttttac
      241 t
//