LOCUS       AY692649                 309 bp    DNA     linear   PLN 24-APR-2007
DEFINITION  Saccharomyces cerevisiae clone FLH114136.01X YNL300W gene, complete
            cds.
ACCESSION   AY692649
VERSION     AY692649.1
KEYWORDS    Yeast ORF Project.
SOURCE      Saccharomyces cerevisiae (brewer's yeast)
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
            Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
            Saccharomyces.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Hu,Y., Rolfs,A., Bhullar,B., Murthy,T.V., Zhu,C., Berger,M.F.,
            Camargo,A.A., Kelley,F., McCarron,S., Jepson,D., Richardson,A.,
            Raphael,J., Moreira,D., Taycher,E., Zuo,D., Mohr,S., Kane,M.F.,
            Williamson,J., Simpson,A., Bulyk,M.L., Harlow,E., Marsischky,G.,
            Kolodner,R.D. and LaBaer,J.
  TITLE     Approaching a complete repository of sequence-verified
            protein-encoding clones for Saccharomyces cerevisiae
  JOURNAL   Genome Res. 17 (4), 536-543 (2007)
   PUBMED   17322287
REFERENCE   2  (bases 1 to 309)
  AUTHORS   Marsischky,G., Rolfs,A., Richardson,A., Kane,M., Baqui,M.,
            Taycher,E., Hu,Y., Vannberg,F., Weger,J., Kramer,J., Moreira,D.,
            Kelley,F., Zuo,D., Raphael,J., Hogle,C., Jepson,D., Williamson,J.,
            Camargo,A., Gonzaga,L., Vasconcelos,A.T., Simpson,A., Kolodner,R.,
            Harlow,E. and LaBaer,J.
  TITLE     Creation of the YFLEX clone resource: cloning of Saccharomyces
            cerevisiae ORFs in the Gateway recombinational cloning system
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 309)
  AUTHORS   Marsischky,G., Rolfs,A., Richardson,A., Kane,M., Baqui,M.,
            Taycher,E., Hu,Y., Vannberg,F., Weger,J., Kramer,J., Moreira,D.,
            Kelley,F., Zuo,D., Raphael,J., Hogle,C., Jepson,D., Williamson,J.,
            Camargo,A., Gonzaga,L., Vasconcelos,A.T., Simpson,A., Kolodner,R.,
            Harlow,E. and LaBaer,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUL-2004) Biological Chemistry and Molecular
            Pharmacology, Harvard Institute of Proteomics, 320 Charles st.,
            Cambridge, MA 02141, USA
COMMENT     This clone is part of a collection of Saccharomyces cerevisiae
            full-length ORF clones generated by the Harvard Institute of
            Proteomics. Each CDS has been cloned with its native stop-codon.
            The CDS has been directionally cloned using the Gateway  cloning
            system into the donor vectors pDONR 201 or pDONR 221. Additional
            sequences in the clone:  'TCCAGCTGACCACC' after the attL1 site and
            before the 'ATG' (from Research Genetics primers used to amplify
            the ORFs, including a Kozak consensus sequence);
            'ATCCCCGGGAATTGCCATG' after the stop codon and before the attL2
            site (from the Research Genetics primers used to amplify the ORFs).
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Saccharomyces cerevisiae"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:4932"
                     /clone="FLH114136.01X"
                     /lab_host="DH5alpha T1 resistant"
     mRNA            <1..>309
                     /product="YNL300W"
     CDS             1..309
                     /codon_start=1
                     /product="YNL300W"
                     /protein_id="AAT92668.1"
                     /translation="MKFSTLSTVAAIAAFASADSTSDGVTYVDVTTTPQSTTSMVSTV
                     KTTSTPYTTSTIATLSTKSISSQANTTTHEISTYVGAAVKGSVAGMGAIMGAAAFALL
                     "
BASE COUNT           74 a           97 c           54 g           84 t
ORIGIN      
        1 atgaaattct ctactctctc caccgttgct gccattgccg catttgcttc cgcagattcc
       61 acctctgatg gtgtcactta cgtagatgtt accaccaccc cacaaagtac tacatctatg
      121 gtctccaccg tgaaaactac ttccactcca tacactacaa gtaccattgc cactctatcc
      181 actaaatcta tcagtagcca agctaacacc accacccatg agatcagcac atacgtcggc
      241 gctgccgtta aaggctccgt tgcgggtatg ggtgccatca tgggtgctgc tgcctttgct
      301 ttgttataa
//