LOCUS       AY692639                1302 bp    DNA     linear   PLN 24-APR-2007
DEFINITION  Saccharomyces cerevisiae clone FLH113379.01X YLR446W gene, complete
            cds.
ACCESSION   AY692639
VERSION     AY692639.1
KEYWORDS    Yeast ORF Project.
SOURCE      Saccharomyces cerevisiae (brewer's yeast)
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
            Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
            Saccharomyces.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Hu,Y., Rolfs,A., Bhullar,B., Murthy,T.V., Zhu,C., Berger,M.F.,
            Camargo,A.A., Kelley,F., McCarron,S., Jepson,D., Richardson,A.,
            Raphael,J., Moreira,D., Taycher,E., Zuo,D., Mohr,S., Kane,M.F.,
            Williamson,J., Simpson,A., Bulyk,M.L., Harlow,E., Marsischky,G.,
            Kolodner,R.D. and LaBaer,J.
  TITLE     Approaching a complete repository of sequence-verified
            protein-encoding clones for Saccharomyces cerevisiae
  JOURNAL   Genome Res. 17 (4), 536-543 (2007)
   PUBMED   17322287
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Marsischky,G., Rolfs,A., Richardson,A., Kane,M., Baqui,M.,
            Taycher,E., Hu,Y., Vannberg,F., Weger,J., Kramer,J., Moreira,D.,
            Kelley,F., Zuo,D., Raphael,J., Hogle,C., Jepson,D., Williamson,J.,
            Camargo,A., Gonzaga,L., Vasconcelos,A.T., Simpson,A., Kolodner,R.,
            Harlow,E. and LaBaer,J.
  TITLE     Creation of the YFLEX clone resource: cloning of Saccharomyces
            cerevisiae ORFs in the Gateway recombinational cloning system
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 1302)
  AUTHORS   Marsischky,G., Rolfs,A., Richardson,A., Kane,M., Baqui,M.,
            Taycher,E., Hu,Y., Vannberg,F., Weger,J., Kramer,J., Moreira,D.,
            Kelley,F., Zuo,D., Raphael,J., Hogle,C., Jepson,D., Williamson,J.,
            Camargo,A., Gonzaga,L., Vasconcelos,A.T., Simpson,A., Kolodner,R.,
            Harlow,E. and LaBaer,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUL-2004) Biological Chemistry and Molecular
            Pharmacology, Harvard Institute of Proteomics, 320 Charles st.,
            Cambridge, MA 02141, USA
COMMENT     This clone is part of a collection of Saccharomyces cerevisiae
            full-length ORF clones generated by the Harvard Institute of
            Proteomics. Each CDS has been cloned with its native stop-codon.
            The CDS has been directionally cloned using the Gateway  cloning
            system into the donor vectors pDONR 201 or pDONR 221. Additional
            sequences in the clone:  'TCCAGCTGACCACC' after the attL1 site and
            before the 'ATG' (from Research Genetics primers used to amplify
            the ORFs, including a Kozak consensus sequence);
            'ATCCCCGGGAATTGCCATG' after the stop codon and before the attL2
            site (from the Research Genetics primers used to amplify the ORFs).
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Saccharomyces cerevisiae"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:4932"
                     /clone="FLH113379.01X"
                     /lab_host="DH5alpha T1 resistant"
     mRNA            <1..>1302
                     /product="YLR446W"
     CDS             1..1302
                     /codon_start=1
                     /product="YLR446W"
                     /protein_id="AAT92658.1"
                     /translation="MTIESTLARELESLILPADSIVNVVDQFQEELLSRLQTNTISML
                     PQCLVPDERSRWNPEDKILTIDFGGTRLKFAIISLPQIVIEYNDAFELTYNIVDSNFF
                     NQIIYTICTRLAANGYIKKKNESSEASKFFVSVTFSFPLNPEGEVVAMGKGFVMTDTL
                     QGSTVKQLIQSSFHRIISENIEEFFCTMNVCHVINDAIAVSLTSKFICENDSISLIIG
                     TGTNACFEVPYGYLPPFKRDALRETLPSSYNKETLNFKHVLINSEIGFIGKNVIALQP
                     FDIHGAISYEMPLECVTSGKWLPLSLKNILLQYNIIPKNFPVEFNGELVCQLAEDCTN
                     AWFENEHYALICQIARLLIKRAAFYVAAIVQAIDIITGCKNYNFIHIGYVGSFLHNSN
                     FYREQIKYYSSIHIKLQFLNHSNLLGAAIATYLNKSDNQVQ"
     variation       154
                     /note="compared to SGD sequence"
                     /replace="a"
BASE COUNT          426 a          263 c          229 g          384 t
ORIGIN      
        1 atgacaattg aaagcactct agctcgggaa ttagaaagct tgattttacc cgctgattcc
       61 atagtgaatg tagttgacca attccaagag gagttgttat cacggcttca gacaaatacg
      121 atttctatgc tcccgcagtg tttagtccca gatgaacgat cgcgatggaa ccctgaagac
      181 aaaattctga ccattgattt cggcgggaca agactgaaat ttgcaattat ctctctgccc
      241 caaatcgtta tcgaatacaa tgatgccttc gaacttacct acaacatcgt tgattcgaat
      301 ttctttaatc aaatcatcta tacaatatgt accaggctgg ctgcaaatgg ttatataaaa
      361 aaaaagaacg agtcatctga ggcgtctaaa ttttttgttt ccgtgacatt cagttttcct
      421 ctaaatccag aaggggaggt agttgcaatg ggaaaaggat ttgtgatgac tgacaccctt
      481 caaggatcca cggtgaaaca gctcatccaa tcttcctttc accggattat atcagaaaat
      541 atcgaagaat ttttctgtac catgaacgtg tgtcatgtaa taaacgatgc aattgcagtt
      601 tcactaacaa gcaaatttat atgcgaaaac gattccatct cattaataat aggaactggt
      661 acaaatgcat gttttgaggt tccatacgga tatttaccac ctttcaagag agatgccctg
      721 agagaaacat taccgtcaag ctataataag gagacattaa atttcaaaca tgttctcatt
      781 aattctgaaa ttggctttat tggtaaaaat gtcattgcat tgcaaccgtt tgacattcat
      841 ggagcaatct catacgaaat gccactagag tgcgttactt ctggtaaatg gcttccccta
      901 tcattaaaaa atattctatt gcaatataac ataatcccca aaaacttccc agtagagttc
      961 aatggggaac tggtatgcca gctggcagaa gactgtacta atgcttggtt cgaaaacgaa
     1021 cattatgcac taatttgtca gatcgcacga ctattaatca agagagccgc attttatgtt
     1081 gctgcaattg tacaagctat tgatatcatc acaggctgca aaaactataa ctttattcat
     1141 attggctacg ttggatcctt tctccacaat tcaaattttt atcgagagca gataaaatat
     1201 tactccagca tacatattaa gttgcaattc ttaaatcaca gtaatcttct aggcgctgca
     1261 atagccacct acttgaacaa atcagacaac caagttcaat aa
//