LOCUS       AY074347                 915 bp    mRNA    linear   PLN 18-SEP-2002
DEFINITION  Arabidopsis thaliana putative E2, ubiquitin-conjugating enzyme UBC5
            (At1g63800) mRNA, complete cds.
ACCESSION   AY074347
VERSION     AY074347.1
KEYWORDS    FLI_CDNA.
SOURCE      Arabidopsis thaliana (thale cress)
  ORGANISM  Arabidopsis thaliana
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
            Pentapetalae; rosids; malvids; Brassicales; Brassicaceae;
            Camelineae; Arabidopsis.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Yamada,K., Liu,S.X., Sakano,H., Pham,P.K., Banh,J., Chung,M.K.,
            Goldsmith,A.D., Lee,J.M., Quach,H.L., Toriumi,M., Yu,G., Bowser,L.,
            Carninci,P., Chen,H., Cheuk,R., Hayashizaki,Y., Ishida,J.,
            Jones,T., Kamiya,A., Karlin-Neumann,G., Kawai,J., Kim,C., Lam,B.,
            Lin,J., Miranda,M., Narusaka,M., Nguyen,M., Palm,C.J., Sakurai,T.,
            Satou,M., Seki,M., Shinn,P., Southwick,A., Shinozaki,K.,
            Davis,R.W., Ecker,J.R. and Theologis,A.
  TITLE     Arabidopsis Full Length cDNA Clones
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 915)
  AUTHORS   Yamada,K., Banh,J., Chan,M.M., Chang,C.H., Chang,E., Dale,J.M.,
            Deng,J.M., Goldsmith,A.D., Lee,J.M., Onodera,C.S., Quach,H.L.,
            Tang,C.C., Toriumi,M., Wu,H.C., Yamamura,Y., Yu,G., Bowser,L.,
            Carninci,P., Chen,H., Cheuk,R., Hayashizaki,Y., Ishida,J.,
            Jones,T., Kamiya,A., Karlin-Neumann,G., Kawai,J., Kim,C., Lam,B.,
            Lin,J., Meyers,M.C., Miranda,M., Narusaka,M., Nguyen,M., Palm,C.J.,
            Sakurai,T., Satou,M., Seki,M., Shinn,P., Southwick,A.,
            Shinozaki,K., Davis,R.W., Ecker,J.R. and Theologis,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JAN-2002) Plant Gene Expression Center, 800 Buchanan
            Street, Albany, CA 94710, USA
COMMENT     RIKEN Genomic Sciences Center (GSC) members carried out the
            collection and clustering of RAFL cDNAs (RAFL cDNA : 'RIKEN
            Arabidopsis Full-Length cDNA'): Seki,M., Narusaka,M., Ishida,J.,
            Satou,M., Kamiya,A., Sakurai,T., Carninci,P., Kawai,J.,
            Hayashizaki,Y. and Shinozaki,K.
            
            The Salk, Stanford, PGEC (SSP) Consortium members carried out the
            sequencing and annotation of the RAFL cDNAs: Yamada,K., Banh,J.,
            Chan,M.M., Chang,C.H., Chang,E., Dale,J.M., Deng,J.M.,
            Goldsmith,A.D., Lee,J.M., Onodera,C.S., Quach,H.L., Tang,C.C.,
            Toriumi,M., Wu,H.C., Yamamura,Y., Yu,G., Bowser,L., Chen,H.,
            Cheuk,R., Jones,T., Karlin-Neumann,G., Kim,C., Lam,B., Lin,J.,
            Meyers,M.C., Miranda,M., Nguyen,M., Palm,C.J., Shinn,P.,
            Southwick,A., Davis,R.W., Ecker,J.R. and Theologis,A.
            
            Yamada,K. (SSP/PGEC) and Seki,M. (RIKEN GSC) contributed equally to
            this work.  Shinozaki,K. (RIKEN GSC) and Theologis,A. (SSP/PGEC)
            contributed equally to this work as PIs.
            
            Annotation is based on the January 2002 version of the Arabidopsis
            genome submitted to GenBank.
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Arabidopsis thaliana"
                     /mol_type="mRNA"
                     /db_xref="taxon:3702"
                     /chromosome="1"
                     /clone="RAFL05-20-O07 (R10593)"
                     /ecotype="Columbia"
                     /note="This clone is in a modified pBluescript vector
                     (Lambda ZAP) as a XhoI/SstI insert."
     gene            1..915
                     /gene="At1g63800"
     5'UTR           1..79
                     /gene="At1g63800"
     misc_difference 37..38
                     /gene="At1g63800"
                     /note="not present in genomic sequence"
     CDS             80..637
                     /gene="At1g63800"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /codon_start=1
                     /product="putative E2, ubiquitin-conjugating enzyme UBC5"
                     /protein_id="AAL67043.1"
                     /translation="MSSPSKRREMDLMKLMMSDYKVEMINDGMQEFFVEFSGPKDSIY
                     EGGVWKIRVELPDAYPYKSPSVGFITKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNV
                     FETFLPQLLLYPNPSDPLNGEAAALMMRDRPTYEQRVKEYCEKYAKPRADTEEMSSDD
                     EMSEDEYASDGDDEDDVAIAGKLDP"
     3'UTR           638..915
                     /gene="At1g63800"
     misc_difference 898
                     /gene="At1g63800"
                     /note="compared to genomic sequence"
                     /replace="g"
BASE COUNT          295 a          141 c          201 g          278 t
ORIGIN      
        1 acattatctt atttctccac ttgattccca gaagacaaaa aaaaaaaaaa aatcagaaaa
       61 aaagaagaag agagaaacca tgtcttcgcc gagcaagcgt agagaaatgg atttgatgaa
      121 actgatgatg agtgactaca aggtggagat gatcaatgat ggcatgcaag agttctttgt
      181 cgaattcagt ggacccaaag acagtatata tgagggaggt gtgtggaaga taagagttga
      241 acttcctgat gcttatcctt ataaatctcc atctgttggt ttcattacca aaatatacca
      301 ccctaatgtc gatgaaatgt caggttctgt ttgtctagat gttattaacc agacctggag
      361 cccgatgttc gacctggtga atgtgttcga gacatttctt ccgcaacttc ttctgtatcc
      421 gaatccgtca gatccattga atggtgaagc agctgcattg atgatgcgtg atcgtcctac
      481 ctatgaacag agagttaaag agtattgtga gaaatacgca aagccaaggg cggatactga
      541 ggaaatgtct agtgatgatg aaatgagtga agatgaatat gcctcggatg gtgatgatga
      601 agatgatgtt gcaatcgccg gtaaacttga tccttaatcc cgcattggtc ttgatatgaa
      661 tcatgtagct ggcttaatag atggctaaac cggggatgac aaaaagaagc tggtgacttt
      721 gtatgtatca tgtatatatg aataatcaat gaatgtttaa aggttccagt tttaattgaa
      781 ctgtaaaatc tctatttctc ttgttgttta cgttgtcatt ttatggtcgt ttaatgtaaa
      841 ttattgaagc ttttttaatc ttcatgttgt gcacctctgt tcaaagtata gtttgttata
      901 aaaaaaaaaa aaaaa
//