LOCUS       AY057445                1983 bp    DNA     linear   BCT 08-APR-2005
DEFINITION  Escherichia coli UDP-D-glucuronate dehydrogenase (arnA) gene,
            complete cds.
ACCESSION   AY057445
VERSION     AY057445.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Breazeale,S.D., Ribeiro,A.A. and Raetz,C.R.
  TITLE     Oxidative decarboxylation of UDP-glucuronic acid in extracts of
            polymyxin-resistant Escherichia coli. Origin of lipid a species
            modified with 4-amino-4-deoxy-L-arabinose
  JOURNAL   J. Biol. Chem. 277 (4), 2886-2896 (2002)
   PUBMED   11706007
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Breazeale,S.D., Ribeiro,A.A., McClerren,A.L. and Raetz,C.R.
  TITLE     A formyltransferase required for polymyxin resistance in
            Escherichia coli and the modification of lipid A with
            4-Amino-4-deoxy-L-arabinose. Identification and function oF
            UDP-4-deoxy-4-formamido-L-arabinose
  JOURNAL   J. Biol. Chem. 280 (14), 14154-14167 (2005)
   PUBMED   15695810
REFERENCE   3  (bases 1 to 1983)
  AUTHORS   Breazeale,S.D. and Raetz,C.R.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2001) Biochemistry, Duke University Medical
            Center, Box 3711, Durham, NC 27710, USA
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
     gene            1..1983
                     /gene="arnA"
     CDS             1..1983
                     /gene="arnA"
                     /function="N-10-formyltetrahydrofolate +
                     UDP-4-amino-4-deoxy-L-arabinose = tetrahydrofolate +
                     UDP-4-deoxy-4-formamido-L-arabinose"
                     /note="ArnA; formyltransferase; catalyzes the first and
                     third steps of the 4-amino-4-deoxy-L-arabinose
                     modification of lipid A pathway"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-D-glucuronate dehydrogenase"
                     /protein_id="AAL23678.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPDVIFSFYYRHLIYDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
BASE COUNT          476 a          500 c          514 g          493 t
ORIGIN      
        1 atgaaaaccg tcgtttttgc ctaccacgat atgggatgcc tcggtattga agccctgctg
       61 gctgccggtt acgaaattag cgccattttt acccatactg ataatcccgg tgaaaaagcc
      121 ttttatggtt cggtggctcg tctggcggcg gaaagaggca ttccggttta tgcgccggat
      181 aacgttaatc atccgctgtg ggtggaacgc attgcccaac tgtcgccaga tgtgattttc
      241 tctttttatt atcgccatct tatttacgac gaaattttgc agctcgctcc cgcaggtgca
      301 tttaatctgc atggttcgct gttaccaaaa tatcgtggtc gcgcgccgct gaactgggtg
      361 ctggtcaacg gtgaaacgga aactggcgtt acattgcacc gaatggtgaa acgtgccgat
      421 gccggggcca ttgtggcgca actgcgcatt gccattgcgc cagacgatat cgctattacg
      481 ctgcatcata aattgtgcca tgccgcgcgc cagctactgg aacagacatt acccgccatt
      541 aaacacggta atattctgga aatcgcccag cgcgaaaacg aagccacctg ttttggtcgc
      601 agaacgccgg atgacagttt ccttgaatgg cataaaccgg catccgtact gcacaacatg
      661 gtacgtgccg ttgccgatcc gtggccgggt gccttcagct atgttggcaa tcagaaattc
      721 accgtctggt cgtcgcgtgt tcatcctcat gccagcaaag cacagccggg gagcgtgatt
      781 tctgttgcgc cactgctgat tgcctgtggc gatggcgcgc tggaaatcgt caccggacag
      841 gcgggcgacg gcattactat gcagggctcg caattagcgc agacgctggg cctggtgcaa
      901 ggttcacgct tgaatagcca gcctgcctgc accgcccgac gccgtacccg ggtactcatc
      961 ctcggggtga atggctttat tggcaaccat ctgacagaac gcctgctgcg cgaagatcat
     1021 tatgaagttt acggtctgga tattggcagc gatgcgataa gccgttttct gaatcatccg
     1081 cattttcact ttgttgaagg cgatatcagt attcattccg aatggattga gtatcatgtc
     1141 aaaaaatgtg atgtcgtctt gccgctggtg gcgatagcca cgccgattga atatacccgc
     1201 aacccgctgc gcgtatttga actcgatttt gaagagaatc tgcgcattat ccgctactgc
     1261 gtgaagtacc gtaagcgaat catcttcccg tcaacttcag aagtttatgg gatgtgtagc
     1321 gataaatact tcgatgagga ccattctaat ttaatcgtcg gcccggtgaa taaaccacgc
     1381 tggatttatt cggtatcaaa acaattactt gatcgggtga tctgggccta tggcgaaaaa
     1441 gagggtttac agttcaccct cttccgcccg tttaactgga tgggaccacg actggataac
     1501 cttaatgcag cgcgaattgg cagctcccgc gctattacgc aactcattct caatctggta
     1561 gaaggttcac cgattaagct gattgatggc ggaaaacaaa aacgctgctt tactgatatt
     1621 cgcgatggta tcgaggcgtt ataccgcatt atcgaaaatg cgggaaatcg ctgcgacggt
     1681 gaaattatca acattggcaa tcctgagaac gaagcgagca ttgaggaact gggcgagatg
     1741 ctgctggcga gcttcgaaaa acatccgctg cgccatcatt tcccaccgtt tgcgggcttt
     1801 cgcgttgtcg aaagtagcag ctactacggc aaaggatatc aggacgtaga gcatcgtaaa
     1861 ccgagcatcc gcaatgccca ccgctgcctg gactgggagc cgaaaattga tatgcaggaa
     1921 accatcgacg aaacgctgga tttcttcctg cgcaccgttg atcttacgga taaaccatca
     1981 tga
//