LOCUS       AV262886                 268 bp    mRNA    linear   EST 06-SEP-2005
DEFINITION  Mus musculus adult male testis cDNA, RIKEN full-length enriched
            library, clone:4930432K07, 3' end partial sequence, similar to
            ddbj:U15176 Rattus norvegicus Na,K-ATPase alpha subunit mRNA.
ACCESSION   AV262886
VERSION     AV262886.1
KEYWORDS    EST; 3'-end sequence (3'-EST).
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Konno,H., Aizawa,K., Akahira,S., Akiyama,J., Carninci,P., Endo,T.,
            Fukuda,S., Fukunishi,Y., Hara,A., Hayatsu,N., Hirozane,T.,
            Hori,F., Ishii,Y., Ishikawa,T., Itoh,M., Izawa,M., Kadota,K.,
            Kagawa,I., Kai,C., Kawai,J., Kikuchi,N., Kojima,Y., Koya,S.,
            Kusakabe,M., Matsuyama,T., Miki,R., Mizuno,Y., Nakamura,M.,
            Oda,H., Okazaki,Y., Owa,C., Ozawa,Y., Saito,H., Sano,M., Sato,K.,
            Shibata,K., Shibata,Y., Shigemoto,Y., Shiraki,T., Sogabe,Y.,
            Sugahara,Y., Suzuki,H., Suzuki,H., Takahashi,F., Tateno,M.,
            Tominaga,N., Tsunoda,Y., Watahiki,A., Watanabe,S., Yamamura,T.,
            Yasunishi,A., Yokota,T., Yoshiki,A., Yoshino,M., Muramatsu,M. and
            Hayashizaki,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-1999)
            Contact:Yoshihide Hayashizaki
            The Institute of Physical and Chemical Research (RIKEN), Omics
            Science Center, RIKEN Yokohama Institute; 1-7-22 Suehiro-cho,
            Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
            URL    :http://www.osc.riken.jp/
REFERENCE   2
  AUTHORS   
  CONSRTM   The FANTOM Consortium, Riken Genome Exploration Research Group and
            Genome Science Group (Genome Network Project Core Group)
  TITLE     The Transcriptional Landscape of the Mammalian Genome
  JOURNAL   Science 309, 1559-1563 (2005)
REFERENCE   3
  AUTHORS   
  CONSRTM   RIKEN Genome Exploration Research Group and Genome Science Group
            (Genome Network Project Core Group) and the FANTOM Consortium
  TITLE     Antisense Transcription in the Mammalian Transcriptome
  JOURNAL   Science 309, 1564-1566 (2005)
REFERENCE   4
  AUTHORS   Carninci,P. and Hayashizaki,Y.
  TITLE     High-efficiency full-length cDNA cloning
  JOURNAL   Meth. Enzymol. 303, 19-44 (1999)
REFERENCE   5
  AUTHORS   Sasaki,N., Izawa,M., Watahiki,M., Ozawa,K., Tanaka,T., Yoneda,Y.,
            Matsuura,S., Carninci,P., Muramatsu,M., Okazaki,Y. and
            Hayashizaki,Y.
  TITLE     Transcriptional sequencing: A method for DNA sequencing using RNA
            polymerase
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 95, 3455-3460 (1998)
REFERENCE   6
  AUTHORS   Itoh,M., Kitsunai,T., Akiyama,J., Shibata,K., Izawa,M., Kawai,J.,
            Tomaru,Y., Carninci,P., Shibata,Y., Ozawa,Y., Muramatsu,M.,
            Okazaki,Y. and Hayashizaki,Y.
  TITLE     Automated filtration-based high-throughput plasmid preparation
            system
  JOURNAL   Genome Res. 9, 463-470 (1999)
COMMENT     Please visit our web site (http://www.osc.riken.jp/) for further
            details.
FEATURES             Location/Qualifiers
     source          1..268
                     /clone="4930432K07"
                     /db_xref="taxon:10090"
                     /dev_stage="adult"
                     /mol_type="mRNA"
                     /note="cDNA library was prepared and sequenced in Mouse
                     Genome Encyclopedia Project of Genome Exploration
                     Research Group in Riken Genomic Sciences Center and
                     Genome Science Laboratory in RIKEN. Division of
                     Experimental Animal Research in Riken contributed to
                     prepare mouse tissues. 1st strand cDNA was primed with a
                     primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN
                     3'], cDNA was prepared by using trehalose
                     thermo-activated reverse transcriptase and subsequently
                     enriched for full-length by cap-trapper. cDNA went
                     through one round of normalization to Rot = 10.0 and
                     subtraction to Rot = 100.0. Second strand cDNA was
                     prepared with the primer adapter of sequence [5'
                     GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA
                     was cloned into the XhoI and BamHI sites. Vector: a
                     modified pBluescript KS(+) after bulk excision from
                     Lambda FLC I. Cloning sites, 5' end: SalI; 3' end: BamHI.
                     Host: DH10B."
                     /note="clone_lib: RIKEN full-length enriched"
                     /organism="Mus musculus"
                     /sex="male"
                     /strain="C57BL/6J"
                     /tissue_type="testis"
BASE COUNT           77 a           44 c           72 g           75 t
ORIGIN      
        1 ttcttcatac gacatttttt tcacgagagc caggattgag tataacaaag acacctgaga
       61 tagacatgag caggagccca tgttctatgg gttgaattga ttatggatga gagagacaca
      121 tttatggggc tggaggaaga ttttttggta ggagatggaa taggttggga gaaagaaagc
      181 ccagcctgtt ctgcctaggt tccagcgagt atgtgcattt ttgttacatc cagcagcctg
      241 tttagagata aatgcttgga acaaccct
//