LOCUS AQIB01109273 15680 bp DNA linear PRI 17-MAY-2013 DEFINITION Chlorocebus sabaeus isolate 1994-021 Contig17.14801, whole genome shotgun sequence. ACCESSION AQIB01109273 AQIB01000000 VERSION AQIB01109273.1 DBLINK BioProject: PRJNA168621 BioSample: SAMN01760484 KEYWORDS WGS. SOURCE Chlorocebus sabaeus (Cercopithecus sabaeus) ORGANISM Chlorocebus sabaeus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Cercopithecidae; Cercopithecinae; Chlorocebus. REFERENCE 1 (bases 1 to 15680) AUTHORS Warren,W. and Wilson,R.K. CONSRTM International Chlorocebus aethiops sabeus Genome Analysis Consortium TITLE Direct Submission JOURNAL Submitted (17-APR-2013) Washington University School of Medicine, The Genome Institute, 4444 Forest Park, St. Louis, MO 63108, USA REFERENCE 2 (bases 1 to 15680) AUTHORS Warren,W. and Wilson,R.K. CONSRTM International Chlorocebus aethiops sabeus Genome Analysis Consortium TITLE Direct Submission JOURNAL Submitted (13-MAR-2014) Washington University School of Medicine, The Genome Institute, 4444 Forest Park, St. Louis, MO 63108, USA COMMENT Chlorocebus aethiops sabeus (vervet) Sequence Assembly Release Notes The vervet DNA for shotgun sequencing, and for BAC libraries, is derived from an adult male vervet monkey (Chlorocebus aethiops sabeus; animal id 1994-021) within the vervet research colony housed a the Wake Forest Primate Facility to create the BAC library CHORI-252. A total of 362,969 BAC end sequences have been generated from this library. A total of 143 CHORI-252 BACs (approx. 18Mb) have been finished and submitted to Genbank. Of these 29 were finished and submitted for the MHC region. Whole genome sequences were generated on the Roche 454 Titanium instrument at these coverage levels (vervet genome size of approx 2.9Gb): fragment- 10X, 3kbp- 8X, and 8kbp- 1X. Total sequence genome coverage on the Illumina HiSeq instrument was 95x (45x fragments, 45x 3kb and 5x 8kb). Two independent assemblies were built with the appropriate sequence data, using the ALLPATHS (Broad Institute) and Newbler (Roche) assemblers. Based on superior contig and scaffold contiguity the ALLPATHS assembly was chosen as the reference. The unique sequences from the Newbler assembly were then merged into the ALLPATHS assembly using graph accordance methods (Yao et. al. 2011. Oct. 23 Bioinformatics). Post assembly we integrated 170 finished BACs. These 170 BACs (including the MHC region) were merged into the 1.0 assembly. The top scaffold that each BAC mapped to was identified by MEGABLAST (-e 1e-20 --W 200 --p 98). Contigs of the top scaffold that the BAC mapped to were identified by BLASTN (-W150 --F F). A Perl script was used to create a new contig for each BAC, extend the contig if the 5' and 3' overlapping contigs were longer than the BAC and adjust flanking gaps accordingly. We then sorted scaffolds by decreasing length, assigned new sequence identifiers to contigs and scaffolds, and extended 20-bp and 50-bp gaps to 100-bp as per NCBI's guideline. In the final assembly, referred to as Chlorocebus_sabeus 6.0.3, there were 162,907 contigs with an N50 contig length of 88 kb. There were 2205 supercontigs with the N50 supercontig length of 45 Mb. A total of 2.73 Gb of sequence was assembled in contigs. Including estimated gap sizes, over 2.74Gb were ordered and oriented along chromosomes, 27.6Mb along the CAE*_random chromosomes, with only 18.34 Mb remaining unlocalized. After organizing Chlorocebus_sabeus 6.0.3 into chromosomal AGP files, we labeled this first vervet release as 1.0. ******************************************* Chlorocebus aethiops sabeus Sequence and Assembly Credits DNA source - Dr. Jay Kaplan, Wake Forest Primate Facility, Wake Forest, NC. Genome Sequence - The Genome Institute, Washington University School of Medicine, St Louis, MO and Department of Human Genetics, McGill University, Montreal, Canada. Sequence Assembly - The Genome Institute, Washington University School of Medicine, St Louis, MO. BAC library - Dr. Pieter DeJong, CHORI, Oakland,CA. Assembly curation - Jessica Wasserscheid, Nikoleta Juretic, Dr. Ken Dewar, McGill University, Montreal, QC Canada. LaDeana Hillier, The Genome Institute, Washington University School of Medicine, St Louis, MO. FISH Mapping Data - Mariano Rocchi, Department of Biology, University of Bari, Bari, Italy. cDNA data - RNA sources was Dr. Nelson Freimer, Semel Institute for Neuroscience and Human Behavior, University of California Los Angeles, CA, USA Funding for the sequence characterization of the vervet genome is being provided by NHGRI. Author List: Nelson Freimer, George Weinstock, Richard K. Wilson, Wesley C. Warren ******************************************* Chromosome Lengths: column 1 = chromosome column 2 = chromosome length (including estimated gap sizes) CAE1 126035930 CAE2 90373283 CAE3 92142175 CAE4 91010382 CAE5 75399963 CAE6 50890351 CAE7 135778131 CAE8 139301422 CAE9 125710982 CAE10 128595539 CAE11 128539186 CAE12 108555830 CAE13 98384682 CAE14 107702431 CAE15 91754291 CAE16 75148670 CAE17 71996105 CAE18 72318688 CAE19 33263144 CAE20 130588469 CAE21 127223203 CAE22 101219884 CAE23 82825804 CAE24 84932903 CAE25 85787240 CAE26 58131712 CAE27 48547382 CAE28 21531802 CAE29 24206276 CAEX 130038232 CAEY 6181219 ******************************************* Chlorocebus_sabeus 6.0.3 assembly statistics: *** Contiguity: Contig *** Total contig number: 162907 Total contig bases: 2734267806 bp Average contig length: 16784 bp Maximum contig length: 1051246 bp N50 contig length: 88741 bp N50 contig number: 7870 *** Contiguity: Supercontig *** Total supercontig number: 2205 Average supercontig length: 1240031 bp Maximum supercontig length: 126332868 bp N50 supercontig length: 45002363 bp N50 supercontig number: 19 Scaffolds > 1M: 147 Scaffold 250K--1M: 47 Scaffold 100K--250K: 34 Scaffold 10--100K: 473 Scaffold 5--10K: 235 Scaffold 2--5K: 434 Scaffold 0--2K: 835 ##Genome-Assembly-Data-START## Assembly Method :: ALLPATHS and Newbler v. 13-Feb-2013 Assembly Name :: Chlorocebus_sabeus 1.1 Genome Coverage :: 95x Sequencing Technology :: 454 Titanium; Illumina HiSeq; ABI ##Genome-Assembly-Data-END## FEATURES Location/Qualifiers source 1..15680 /organism="Chlorocebus sabaeus" /mol_type="genomic DNA" /submitter_seqid="Contig17.14801" /isolate="1994-021" /db_xref="taxon:60711" /sex="male" /dev_stage="adult" /geo_loc_name="USA: NC, Wake Forest University" /note="housed at the Wake Forest Primate Facility as part of the Vervet Research Colony" BASE COUNT 5131 a 3197 c 2753 g 4599 t ORIGIN 1 gcaagaacag aaaaccaaac accgcatgtt ctcactcata ggtgggaatt gcacaataag 61 aacacttgga cacaggaagg ggaatgtcac ataccagggt ctgttatggg gtgaggggag 121 gggagaggga tagcattagg agatatgcct aatgtaaatg atgagttaat gggtgcagca 181 caccaacatg gcacatgtat acatatgtaa caaacctgca cattgtgcac atgtacccta 241 gaacatcaag tataacaaga aaaaaaaaaa agaagtacaa gtggaaacca gattgaaagg 301 ggctgatcag aggtaatgga gtcaacaaat gtagacttaa gtctttcaag aacgctgacc 361 ttgaaagaat gggagaaatg acactggttt catcctttcg tgttaaactt gaaagacaga 421 gcgagatgga gcaacatcag gaactatgac ggatcaagaa gatatttaac aggccaggtg 481 tggtggctca cgcctgtaat cccagcactc tgggaggcca aggcaggcgg atcaccaggt 541 caacagatga agaccatccc agccaacatg gtgaaaccct gtctctccta aaaatacaaa 601 aattagccgg gcatagtggt acgtgtctgt agtcccagct actcgagagg ctaaggcagg 661 agaattgctt gaacccagga ggcagaggtt gcagcgagcc aagatcgcac cactgcactc 721 cagcctggtg acagagtgag actctgcctc aatagaaaaa aaaaagattt aataatagag 781 gggaacacag cacattcata gacagaaaga gggagagcca gaagttacaa gaatgaggaa 841 gctaactggt caagcgggat cccagaggta ctagaaggaa tgagaccaaa agcaggtttg 901 cttttgaaaa ggttgatatt tcatctctaa gaggggaaca aaaagttaag aattattaca 961 gacacaagaa acattttgag ggagcaggtt aacaaacact tgttagtacc tacttagtgg 1021 caagtagctc agcagagttt aggaaatggc agcttgaatg aggcaaggtc cctgctctca 1081 gaaaccttac catctgtgga cattaaagaa gtccacactg gacaatggta aatcaggagg 1141 taagcagctg tctgcaaagg acatgaggat agtttgggtg gtgaggaaac tagaaaagac 1201 tcagagcagt gttctgaacc ctggcaccca tcagaacacc ctgaaaagac ttgtgaaagt 1261 ctagatccct atatactaga aaagtctgat tatgcaggct taagcgctat aggcaatctt 1321 aggctctata gggaattctg tctggcaact accttaaaat tactggttta gaaccatccc 1381 tggggaacaa ctagggaaga gccaattaaa gataagtagg ataattttgt gaagaattaa 1441 aatagctaga ttttattatt ggggacctgt aaacatttac catttggaca ttttctttca 1501 agaaattagc agcaccacaa aatgaatcat ataagcagat attctcatat tatgtagtgg 1561 ataaaccttt taggaaaagg tagcattcat ggtaaagtaa caaaatggca gagaaaaaga 1621 agaaattact aaactaaaac tacataaaca catactggtt ggaaaaatat ttcttcaaaa 1681 tcattgaaat ttctcaaata atatccttta aatacactca ggtttcccct atgttggtag 1741 aaaaaaaacc aaaaaacttt attagatgct acttcctctt ctagctagga tcccttctcc 1801 tttcttcact gcctaacttc tagaatgagt ggtctgtgcc cacagcctca atttccttcc 1861 cacccactcc ctcctcaata ccagcaatct cgctgctcca acatctatct actcaaactg 1921 caattttcaa ggtcaccagc ccttttatag tcaagctttt ggcctctttt cagtgctggt 1981 gttcctaagt tgttggtgga ttttaataca attaacactt tcctctcctt ctaaaacccc 2041 tttcctagcc ttcccactta cacccatgca ttcccttctc tgtctccttc cttatcttca 2101 ctgcctcctc ccatatcttt tctgcaagct atctttctcc ttgggcatcc taatgtcagt 2161 attcaccaaa gtctcagctg ctttttctct ttacactttc tctcctcttg ctcctcaatc 2221 ccttgaactt caactaaatc tctacacagg tgatttcctc ttggtttcac cactccagtc 2281 acagtttagc tccctctgtc cttcagtaag cctgtttgac ctgcctggaa tgttcatccc 2341 tcaactcttc ttacctcgct gtttctcttc atcttcatct taaatatttc ttccttggag 2401 aagcctaact tgatcctcaa accagattag actcaccgat tacacatttc tatgtcaccc 2461 tatatttatc cttacgcact tgcacattat ttatttgcag taattgattt aatatctgtc 2521 tttcccagca gatttaaact ccatgagagc aggacctgtg tctgtcttga tcaaatgtcc 2581 acagcatgaa gcacagtgac tgatacatga cagacactct aaattactgg ttgacagaat 2641 aacccgccca cattttagct ttgacttatt tttcctaagc aaaaatctca aacatttagc 2701 taaaatttat ccccacttga atacttgctg tcatttcaaa tgtatcacat ctaaaagtca 2761 tagatttcct cccacacact tgccccatcc ctttgccctc taatttttgt actatgaagg 2821 tgttcaaaac tcagctcata ccccagaaag ctgtgtgata tgattagcaa tcccacatac 2881 acgctcgggc tttatttgtc tcatgtataa aatgaaagat ttttaattag aaggtttcta 2941 aagtacttcc tttctgaagg ctttatactt cttaatagtg tcatacatct ccttcatttc 3001 acaaatgagg aaattactgt caaaagaagt taagcaattt ctaaagatga aacggctagt 3061 ggttagaatt acataactga tctttagaac caacacagag ttatattcgt attagaaatt 3121 ggtatctatt ggtatctaaa ttcgtatcta gaaattttac tagaaattgt ggaaaagaag 3181 aatgaaattt tattgagaaa agtgaaatgt accaaaatat ccaatgcatg gataaaattt 3241 agaaagcaaa aaggcataat aatatattca atcttaacat ccaaaacaat ataaccatta 3301 agtcaatatt agttgtaaaa atacaacaat attgttgtaa aaatacaata atattttaaa 3361 atatgtatat ttgaagaaag tttcacttct ttatgggcca gtgagtttcc acaggtaaga 3421 actataaatt gtggacaaaa tataaaaaca actacccgag gttactggaa agtggaggaa 3481 gcagaaaaat tcgcgaatgg tgtcaatgtt tggaagaagg aaataaggac tcagagttgt 3541 gggtgtaggt tcccattatt tcctcatttt tactgatgtt agccccagag agggctgcag 3601 atgataccac agtaggcagc taaaactaat agaaaaccca cagttttctg aagaaccagc 3661 aggcagattt tagagcaatc ttactctctg aaaagtgata gagtcagagt aaatgagttc 3721 caaattctgc atacactcta cgcatatctc tgatgactct aaaccacaca ttgcaggtca 3781 cggtcaaagc aaattagcta cctgtaaaag aactgagatt tgagatactg ttcaaggggt 3841 agagtttgca gtttgagtcc aaccaagtaa aatatcaaca cagtaatata acagaatctg 3901 tgtaacattc aaaacccaaa taaaactatt taattacaaa gaacaaagaa aatgtggccc 3961 attcttagga gaaaatacat taagggacac caacaacttc aagctgaccc agacactcga 4021 attctcagac aacagtctta aagcaacatt cagctatgct tggtgacata aagaaaaata 4081 tactcacgat gaatgaataa aaacacagaa aaatctcagc agagaaaaaa gaaactttta 4141 acaaataaat ggaaatctta aaatagaaaa atacaatatt tacagaagaa tggagaggag 4201 aaaggaaatt ataattgtca gtgaacttga atatatagca atagaaatta ttcaatcaaa 4261 agaacagaaa gataagatat tggagaaaat atatataaaa agaaaactaa gaactaagag 4321 aactcctcac tagcagacct acactactag caacaataac agaaattatt caggatgaaa 4381 gaaaataata ccaggtagaa acttggaaat tcaagaagga attaagagta ttagaaatga 4441 caactagagt ggacataata tgtgccacca acaattgtct ttgacttttt gttcctgtca 4501 ctaggatcac tgtcagcaga cagtcttcag ttttcagcct ctccagggat gacttcagtt 4561 actttgacca aagtcagacc ctttccaggg gagcccatat ccagtgactg atttagatgg 4621 agatataaag gaccagccat ttttattcaa tgcagggcct ctctaaggcc actctagctc 4681 ctgagctgtg gggtcaggta agggtgtcag ttggttgaga ttgcaggtag acttctccct 4741 ctgctcacaa gcatcctgta cacaaaactc tcagagtcta atttctaaga gaacctgcga 4801 cagtatcagt aagtcaacgt agcagagatt tttaaagaaa attatttctt cgaaagtgga 4861 aaaactggcc aggtacagtg actcatgcct gtaattccaa cactttggga ggccaaggca 4921 ggaggatcac ttgaatccag gagttcagga ccagcctggg caacatggga aaaccctgcc 4981 tctacaaaaa atacaaaaaa aaaaaaaaaa aaaaaattag cctggcatgg tggtgcacat 5041 ctgtagtccc agctacttgg gagtctgaaa caggaggatc acctgagact aggaggtcaa 5101 ggccgcagca agccatgatt gcaccactgc actccagcct gggtgacagg tagagtgaga 5161 ccctatctca aaaaagaaat ggaaaaactt ttagccctgc tttaatcctg gaactctttg 5221 gtatcgcttt ccttaaatac aaaccagatt ttggtcttag aatggggtct aggcatataa 5281 attctagtga gaaaaactct gaagttgctt gcttagtgtc aatgcatgaa caatgtcatt 5341 tcttcctggg aaaaaaaggg ctgattgtga ctgtgcctat tctatgaatt acataaatca 5401 gtgagattta ttactattat tattatttaa aaagtaatga ttgttgactt gattcttagg 5461 gggtactagc atcatacagt ccaaagctat tttataacca cagatctgct cccaaattcc 5521 aatcaggaat tgacaaatgt atgtgagtga caataaggga gaagatgcaa aaatgtgttg 5581 agggttacta cattttaatc aatgtacagg ttaatttttc agacacaaca taaaatcaaa 5641 caacaactgg gtcatttgat taactaaaca ccattaactg aaatattcat gaccttactg 5701 ggaggaaatt tatctactgt aaactctctc atttgttccc attaccttat tgccatcctc 5761 aacctattag catccctata tgtaatgtct catcttaatc ttcaatttca gaagagtatc 5821 tcctcctaca cgaagttaag cactcaactg atactccact ttggctccct tcctgtctct 5881 tctcagatct tcctatcttt tatagactgg aaagagataa gtatggttct tagagcccaa 5941 atccaaatat ctctagtgct tatttttttt tttttttttt tgtccctatg tttattctgt 6001 ttgtgggatg gatcatctac ttcatgaatg attcacaaac actggagttt tctatacagt 6061 catccctcag tgtccttagg ggattagttc taggaccccc agagataaca aaattcaagg 6121 atgctcaaat ccctaatata aaatagtgta gtatttcata taacctgtgc acaatctctc 6181 atatactttc atctctgatt gcttataata cctaatacaa tgtaaataat atacaagtag 6241 ctattatact gaattattta ggaaatgaca acaaaatctg tacatgtaca gtactaatga 6301 aatcatctat tttttccctt tatattttca acagaagttg gttgaatcca gggatgcaat 6361 acagagggct gactgtattt ccaagtatct gcaggaattt tcctcctact tgtcctccct 6421 tcctatactg aacttaacat cttcctccac aacgtaactc ccttctcagt cacatatata 6481 tctcagtggc aacacataac ttttttcaaa tgagaacccc agggtagaag ctcttgactc 6541 atgtttcaaa ataaatattt tacctagtgc cactgccact caatgggaca ctgttatcac 6601 attattctcc aaaactccta ttcaattata aagtttcctg ttcaccaaag aacagatggc 6661 tcctcattgt ctactgacta atacccaggt tcttttattg agctattaag gacccccaaa 6721 atccagcctt aattcctact atcatttaac acacaatctc tgttcctgca agctacggct 6781 ctttctctct ctctgaagca ccttgtattt gcacatgcta cacatcccat ccttccaatt 6841 cctttttctg catttcccca tttatccaaa cctgtcattc aaagtctagc tcaagaccca 6901 tctccacaaa atcttccaca actccaaccc actgtaattt ctccctgctt tagatttgtc 6961 ctagtttcat atacaatttc aagatttact gattttaaat gttcaccaat tgtttcatct 7021 tacctttgtc tttctaattt agattgctaa taacttttag gcactattct acacacacac 7081 acacacacac acatacacac attaataagg acttttaatt aattaattgc ttaagcatga 7141 acatttagag aaaaacacac ctataaagta ttataacgta aattctcaaa tactgcaaac 7201 tagaaaagga agatgggact aaaattatta ataaggacaa aacccttctt ttgcacaatt 7261 gtttttaatc tttttccatt gtttgtaaat ctgccaaagc taccttagag ccctgtctta 7321 atttttatct cctcttgttg ttactgtctg tcctctagtg gaagacaaca ataagtgaat 7381 aaaaattagc ccaaagaagg aaagaagtgg agggtttctt tttagtaact cataatcaca 7441 aaatcagtag cttttataaa agatggaata attttaattg gaacaccaga atataatttg 7501 aacacataca aaaaaatcta gaagacaata gggctaaaag aaggaggaag aagtggcact 7561 gggtatgggg agtgcccagg aaggggacca ggtacaaggt tgggaatatt cctctaatgt 7621 gcacaattta gtttgttttt atttttcccc tcaggttttt ttctggataa atcttgttta 7681 ctaactacca agctgcttga agttataagc aaataagtaa acaaaaatag ggaagccaaa 7741 tgaatactat gttttacatt taaatcatta gctcccttgt tcctttgtga atttatcttg 7801 tacaaagcga gttctttctg tttggcaata ttaataacat agcagtttta gcaataaaga 7861 cataaagtca tttccccaag tggtatgctt ctcattattc taaatcatgt catggtaaac 7921 ctatcacacc aaatcactac atgtaaagta atattttttt tacatcttga tattaaacat 7981 ttaataactt tacaataaca taagcataga cttcatgatc tgatatacgt agtcttttct 8041 agttgtgttc agaaatagtt ttaaaattta aacttttcaa cctttaaaat tgaaaattat 8101 aaattttgtt ttaatcattt ttttttcctt ttcacagaag taacagtgca atgtgacata 8161 aacagctctt gcctatctgg ctgccccagc caagctgggt cctgcacaag tttctataag 8221 agctttctac aacaagatct caactactga aaggttcaga tcactcgact tttttaggtg 8281 aggaacagtc tattaaggct aaaagcatcg gcttgacttt tttcactgat gctcttcggt 8341 gaaaagcaca atggttaaat aatgaggaaa attgcccact caaatgttta gtgttgctta 8401 ctgatgcata tcattgctga ttgtgattct gccgcacact agtcatatct aaccttgggc 8461 aaattatttt acctctctac gcctccgttt ctacctttgt aaaatggaga ggtgtcttcc 8521 atgtctaatt cactgggttg ctgtgtagat caaaggggga aaagcatgtg aaaattctca 8581 atagaaaggt gcagtcatag agtctagtta ttttgcatgt gctcactagt aagattaaca 8641 cgtggaaagc agattttttg ctggaagtca ttcagaaatt aggaaaaata ccttttcttc 8701 tttcaattat caaattataa catttaaaac aaactacact cttcccttgc agctgctccg 8761 tctactaaaa gtgaaacctg aaaactccct cccacaccaa ctcctaacct tctacagtga 8821 aaccaggaca cccccacagt ggcccaagtt ctctataact tctataggaa cctagatgtt 8881 gaagagtgtg ttctctaact cgtagtgtct cttacagccc acacatcttg aagataaata 8941 cagacataca tcacagacac accactgtaa ctgtaagcat attatatttt ttaagtgcct 9001 aatcatcgaa agtgacagaa agcaataaac tgtcctaatc tatgctactg gcaatgtggc 9061 cctaattgag ttagggttct tattttttaa gaaaaaattt aagagagagg aggctgggtg 9121 cagtggctta cacctgtaat cccaatgctt tgggagtctg aggtgggaga attgcttgaa 9181 gccaggagct caagaccagc atgaacaaca tagcaagact ctatctctat caaaaatttt 9241 aaaaattagc caagcatagt ggtacatatc tgtagctcta gacacttggg aggctgagtg 9301 ggaggactgc ttgagccctg gagttacagg ctgcagtgag ccatgattgc accactgcac 9361 gcctgcctgg gcaacagagc aagatcctgt ctcaaaaaag ggggtggggg cagagaataa 9421 gataaagaga acctaagtgc ctttgattgc tgctagtatg tatttgattt atcctgcctt 9481 gttcaaaaaa caggaggaaa taaggaagtg ggcagtaaga caaattatag agatctgtat 9541 attgtagtag gatacaaagt aaaataagca agacaccaga aaaaaggatg ataaagtaaa 9601 ctagatatgg gagtaacccg tatttagcaa ccccctatag ctattattgc aattgatttg 9661 cattgcccca gaagaaagac aaaaaataaa ctcaggaaga tctgagacca ggtttcttac 9721 tgttctccca aagagaattc aaacttacca gccactaaga agacagccaa aatcaacagt 9781 agccctaggt tagcagttct ccaagggcag agcatttctg ttttctcttt ttctgccctt 9841 cactcagtac ttttcctcca cacaggtaca gaaggaactg tgcgcatggt gagaccctct 9901 ctggtcaact tctcggtaca ggatactctt actccatcaa cagcagggca ctcccttcct 9961 tctagaccat tccttcacgt ctatgtggtt tagcctgatt agtaacttgg acgaacgaag 10021 cttttctctg gaacttgaca cagcaataga tttcctgtat gaagccctga agcaaagacg 10081 gaggaagcag attgattgaa ttatctctca gctcattggt tctcctctca cttctgctgt 10141 acacagataa attttacagt aaggcactga acatgtccaa gataaacaga tttttctaaa 10201 cttgtaccaa agagtaaaac agaatattat ctattttggc ccatccttgt aaatttctca 10261 ttcatgataa cttttagagg ctttctccag gttgaagagc tttatggcct tagtattcaa 10321 gtctggaata cagtattttt ccttgaaaat tattgcgtga aattactgtc tataatggga 10381 gtcaacacac aaccctgtaa acatcataag tgtattagct tcctattgct actgtaataa 10441 attaccataa actcagtggc ttaagacaac ataacattta ttatcttaca gttctggtga 10501 tcagaagtct gaaatggacc ttaggggact aaaatcaagg tgttggaagg gctgcactcc 10561 atctgcagac tcgggcagaa tttgttccct gtcttttcca gcgacttgag aatgcctgca 10621 tttcttggct tgtagctata tcattcggac ctctggtcca ttgtcacatc tccttctctg 10681 actgtgaccc ttctgcctct cttttgtaaa taccttggga ttattttcag tccacccaaa 10741 taatccaaca tgatctccta atttcaagat ccttaattta atcacatctg caaagtcctt 10801 tttgccacac aagggaacat attcacaggt ttcagtaatt cagacatagg tatctacctt 10861 attgggggtg agggcattct gtttaacaga actcagtatt cattgccatt cagtttcacc 10921 tttggagaat cagactgatc attttaacca aattacaaat aatcacctat cttttagttg 10981 ccacacatct ggaattcaga cttcccccac caaaaacaaa caaacaaaca aacaaaaaaa 11041 cctcttcctt aatgttgcta gttactacgc ttttaagtta tgtctgagag agggaaaaat 11101 attctgtgtg ctaagaattt tctaccccaa ggcaaaacta tacaggaagg ggaagaaaac 11161 attgcaaaca ggaaaaagat aaaacagcat gcattttttt tttctcagtt agcagtttca 11221 tcttccttct tgccaaacat tataaatatt ttcccccttc cccttctcaa gctttttttt 11281 tccaattaat taatttgatg aagacagtga aatcaggtcc ttgagggcaa ggaactcttg 11341 agggtatgtg ctatgacaat gggaagacca taacaaaaaa tgtcttctct tctgtctaat 11401 gtttcaccag gtgttggaaa attagtgaga atacatcatt taaaatacgg gtaacattct 11461 ggttttccac cacatagcag ttaaagttgg ccgggagtag tggctcatgc ctgtaatcct 11521 agcacattgg gaggccgaag agggcagatt gcctgagctc aggagttcaa gaccagcctg 11581 ggcaacatga caaaactcca aactccatct ctactttaaa aaaaaaaaaa aaaattagcc 11641 aggtgtgatg gcactcatct gtagtcccag ctacttggaa agctgaggca tgagaatcgc 11701 ttgaacccag gaggtagagg ttgcagtgag cagagggaag ttaaagttaa aattcacact 11761 ggtttaggat acagagagac atagggttaa gtttctgttg tgtaattact actccagtga 11821 tactgggaaa gtttcttact caagtttgta tctgtttatg ttatattgac attagctttt 11881 tctcatgtca tcaaaaattc tgggagatca tttattttaa tatctgctca gtgacatagc 11941 caaaaccaaa ccacagaagg gctaaatggc ctagggcctc aaagcgattt gttggcacag 12001 ctgggaataa aatgtgttat attttctagc cctgtgttct tttcactcag ctcctctact 12061 ttgaaatttc aggcaacaac tttcatagac acactccaat gcacttacct gaggtcagac 12121 cacaatctgc aatcagcagt ttaagaatca agtgatgggt ataaaaaacc ataaaattcg 12181 caaaatagat ttggcaggat gacagtttct ctccctggtc cctcatcctc tccaccccca 12241 aaccacacgc ctgtaacctc tcaactccca ccttctacct tttatggacc gtgaatgttt 12301 aaaacaataa agggactaaa agtttgttaa cataatcatc cacgacatgc catgaaagtc 12361 agtataatta gagaaagatg tgtctggtaa atgcacctct ctaaaaaatc taaaggcccc 12421 aaattctccc tggttgggtc ccttgggcac aggacccagg aagcaaagca aaagagtgaa 12481 aaaggatcaa gaaacaggtg tcgttcaagc caaaaaccca aatcttttac tctaccagtt 12541 ttccaattct tcaataatac cagttaagaa tcatagcata agagaaacaa acagaacaat 12601 gtaatgccaa ttaatgttgt agccaaaaca aaaagacaca gaagctttag taaattagaa 12661 ctactttagg ccataatgcc cccaaaccag catggttggt ttactcaatt tattaactgc 12721 aataaactga aaattgatgt aggtacctgt aaatgttgtt gaagtaaact tcttccaatc 12781 aggtgtatat ctttctctcc aatgtcagat tcagacttag ccacactgaa accatttaag 12841 atgaaatgtt gcagccttgc ccttgggatt cctccccatt atgtaggagc tttgcatggc 12901 tcttgaatac ttactgcaaa gactctatgg acttttattc ctgacttctt gttccctgga 12961 ccctttgttc cccagtacca ctgcctactc attcacctct tatatttgag tcttgactgc 13021 cgagaacttc agcaccttcc tgcccttcct accatagcta aattttctgg atgtgattct 13081 tgcaaaatgg gccaataact acttgccttg tcactgttac ttttggtgaa ctctctggct 13141 tagacatctg gcctgactta cccttctata cacatgcatg tgtgcacaca caaacataca 13201 cacacatact cacacaaata tactagctaa acacttagtc ctaattccct caatctactc 13261 tgtcctgcta gccaagtggt aatatatcaa tttcacagag gtaaaaagaa caatacctaa 13321 tagtctaaag gattttaaag ggactcaaac tctaaaacac tctactcttt acttcctctc 13381 tgcccccttg tacaataacc acagatttga atctaatgaa ttaccacaga aatgcgataa 13441 ttcgacatac aattgcataa aaagacagct ttcctggcca tattccattt tctattttcc 13501 aattcacctc tctgtaacct tcattgaccc atgctctcaa ggctcatctg aaatcctgta 13561 tcttcctcca tggacaatca ttaaatggac taaatggtaa cttaaaagag cctcaaaaaa 13621 tgcatatttc tatttttata cctactatta tattgatttt tcttgacatt ttttattcct 13681 aatatatggc tgatctatgt cacatgcata aaaattattc ttaaaaagaa aaaatatatc 13741 atattggaat ttattattat tttttcatta taattttaca gattatacaa aaaggaaaat 13801 tgagaaatag atgtttgaaa atcaaatgtg tacagtatca aaataagcaa aatcagagta 13861 gagaaattag taaccacatt ttatatttag ctaaatatta actgtaaatc tcactggtca 13921 gatccatagc aaatacttgt caagccctaa gcttaatacc ctggtcttgt tctaagcagt 13981 ttataaatat tacctaattt aattatctca acaatcctat atagtaaata ccatttacta 14041 ttgaggaaac tcaaagcaga gagcttatgt aaatcgtcaa aagccacaca gttgataagt 14101 gatagaaaca ggattcactc caaagtgaga tttcttaagt ctacttctta agctatgtgg 14161 ctaattagtc tctcctttac tatctacttt ttgaaggtgg taactctgtt ttgtctccaa 14221 tacatccccc atattcctaa tccctagtga atctatttat gtattcattc actctttctt 14281 ccttaccttc tcataattgg tctgttttgt ttaaacaaat aagcaaaccc tgtgctgttt 14341 ctaatcctct atttgcatta agattactct ctgctgcccc ctggagataa tttgatgaaa 14401 ccaatgctaa gatttttgta aatttatcta gcaaattcaa aaatagctgc acattaagat 14461 tttgatcaca aaggatgcta acatgcctaa acattataca tttaatttac atcttttgga 14521 aactgctgaa acataacaaa tgactacctg atggatagta cagatatcat accttgaaga 14581 aatgttgctg aaaaaaataa aaatgaatct tttgagttgc caggatggtt taataaaaac 14641 aacagtgctg ccaggcatgg tggctcatgc ctgtattccc aacactttgg gaagttgata 14701 caggaggatc acttgagccc aggagtttga gacgagcctg ggcaacatag tgacagccca 14761 tctctataga atttaaaaat tgttttaaaa gcagcagtgt aaagaaatgg ataaatagat 14821 ggatggatgg atggatggat ggatggatgg atggatggat ggatggataa aagatagatt 14881 ccctacctca ttgcatgaaa atttgtgaag actgccactt ttgggtaaac tggaatgcaa 14941 actccaagat ggagtttaat gtgcaggatt gttattatat taaggagtaa cctcagaaca 15001 tcacttgggg ataagaagga agagaaactg atgtgggcat aggaaatcac actgtacggt 15061 agccccacac cagactcagc taaccctatg gggagctata gagctgaaac tgctccttag 15121 agttgtcctc tgttgtcctg aaacagacag atctttacac ttctgcatct atgagtcatt 15181 tgttatagcc acaggaagga ccatgacctt gggtaaggca atccatgaag aggctgaccc 15241 ctgaactgtg gaaaacacta tcaacactga agcaacaaag tcctttactg aagggggttg 15301 atggtacccc acagtgtttg tctgccaatt gaataaatgt tgaaaaataa tctactctaa 15361 ttctagtctc aaatacaatg atattccact cagccaattg tctttcattc ccaagaaacc 15421 aaattaatgg aaaatcttgt caaaaatata accttgaagg agctctgcat taagatattt 15481 cctacctgag gtagtcaggt agttttcatc tgtgtcaact gagaagtcca gaatatatgg 15541 aaataaattt ttgaaaggag aagcccagtc atataccctg gctaaaaact aacatcaagg 15601 gaaagcacgc tgaagacaaa ggagaagccc agtcatatac cctggctaaa aactaacatc 15661 aagggaaagc acgctgaaga //