LOCUS AQIB01010157 16128 bp DNA linear PRI 17-MAY-2013 DEFINITION Chlorocebus sabaeus isolate 1994-021 Contig0.15654, whole genome shotgun sequence. ACCESSION AQIB01010157 AQIB01000000 VERSION AQIB01010157.1 DBLINK BioProject: PRJNA168621 BioSample: SAMN01760484 KEYWORDS WGS. SOURCE Chlorocebus sabaeus (Cercopithecus sabaeus) ORGANISM Chlorocebus sabaeus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Cercopithecidae; Cercopithecinae; Chlorocebus. REFERENCE 1 (bases 1 to 16128) AUTHORS Warren,W. and Wilson,R.K. CONSRTM International Chlorocebus aethiops sabeus Genome Analysis Consortium TITLE Direct Submission JOURNAL Submitted (17-APR-2013) Washington University School of Medicine, The Genome Institute, 4444 Forest Park, St. Louis, MO 63108, USA REFERENCE 2 (bases 1 to 16128) AUTHORS Warren,W. and Wilson,R.K. CONSRTM International Chlorocebus aethiops sabeus Genome Analysis Consortium TITLE Direct Submission JOURNAL Submitted (13-MAR-2014) Washington University School of Medicine, The Genome Institute, 4444 Forest Park, St. Louis, MO 63108, USA COMMENT Chlorocebus aethiops sabeus (vervet) Sequence Assembly Release Notes The vervet DNA for shotgun sequencing, and for BAC libraries, is derived from an adult male vervet monkey (Chlorocebus aethiops sabeus; animal id 1994-021) within the vervet research colony housed a the Wake Forest Primate Facility to create the BAC library CHORI-252. A total of 362,969 BAC end sequences have been generated from this library. A total of 143 CHORI-252 BACs (approx. 18Mb) have been finished and submitted to Genbank. Of these 29 were finished and submitted for the MHC region. Whole genome sequences were generated on the Roche 454 Titanium instrument at these coverage levels (vervet genome size of approx 2.9Gb): fragment- 10X, 3kbp- 8X, and 8kbp- 1X. Total sequence genome coverage on the Illumina HiSeq instrument was 95x (45x fragments, 45x 3kb and 5x 8kb). Two independent assemblies were built with the appropriate sequence data, using the ALLPATHS (Broad Institute) and Newbler (Roche) assemblers. Based on superior contig and scaffold contiguity the ALLPATHS assembly was chosen as the reference. The unique sequences from the Newbler assembly were then merged into the ALLPATHS assembly using graph accordance methods (Yao et. al. 2011. Oct. 23 Bioinformatics). Post assembly we integrated 170 finished BACs. These 170 BACs (including the MHC region) were merged into the 1.0 assembly. The top scaffold that each BAC mapped to was identified by MEGABLAST (-e 1e-20 --W 200 --p 98). Contigs of the top scaffold that the BAC mapped to were identified by BLASTN (-W150 --F F). A Perl script was used to create a new contig for each BAC, extend the contig if the 5' and 3' overlapping contigs were longer than the BAC and adjust flanking gaps accordingly. We then sorted scaffolds by decreasing length, assigned new sequence identifiers to contigs and scaffolds, and extended 20-bp and 50-bp gaps to 100-bp as per NCBI's guideline. In the final assembly, referred to as Chlorocebus_sabeus 6.0.3, there were 162,907 contigs with an N50 contig length of 88 kb. There were 2205 supercontigs with the N50 supercontig length of 45 Mb. A total of 2.73 Gb of sequence was assembled in contigs. Including estimated gap sizes, over 2.74Gb were ordered and oriented along chromosomes, 27.6Mb along the CAE*_random chromosomes, with only 18.34 Mb remaining unlocalized. After organizing Chlorocebus_sabeus 6.0.3 into chromosomal AGP files, we labeled this first vervet release as 1.0. ******************************************* Chlorocebus aethiops sabeus Sequence and Assembly Credits DNA source - Dr. Jay Kaplan, Wake Forest Primate Facility, Wake Forest, NC. Genome Sequence - The Genome Institute, Washington University School of Medicine, St Louis, MO and Department of Human Genetics, McGill University, Montreal, Canada. Sequence Assembly - The Genome Institute, Washington University School of Medicine, St Louis, MO. BAC library - Dr. Pieter DeJong, CHORI, Oakland,CA. Assembly curation - Jessica Wasserscheid, Nikoleta Juretic, Dr. Ken Dewar, McGill University, Montreal, QC Canada. LaDeana Hillier, The Genome Institute, Washington University School of Medicine, St Louis, MO. FISH Mapping Data - Mariano Rocchi, Department of Biology, University of Bari, Bari, Italy. cDNA data - RNA sources was Dr. Nelson Freimer, Semel Institute for Neuroscience and Human Behavior, University of California Los Angeles, CA, USA Funding for the sequence characterization of the vervet genome is being provided by NHGRI. Author List: Nelson Freimer, George Weinstock, Richard K. Wilson, Wesley C. Warren ******************************************* Chromosome Lengths: column 1 = chromosome column 2 = chromosome length (including estimated gap sizes) CAE1 126035930 CAE2 90373283 CAE3 92142175 CAE4 91010382 CAE5 75399963 CAE6 50890351 CAE7 135778131 CAE8 139301422 CAE9 125710982 CAE10 128595539 CAE11 128539186 CAE12 108555830 CAE13 98384682 CAE14 107702431 CAE15 91754291 CAE16 75148670 CAE17 71996105 CAE18 72318688 CAE19 33263144 CAE20 130588469 CAE21 127223203 CAE22 101219884 CAE23 82825804 CAE24 84932903 CAE25 85787240 CAE26 58131712 CAE27 48547382 CAE28 21531802 CAE29 24206276 CAEX 130038232 CAEY 6181219 ******************************************* Chlorocebus_sabeus 6.0.3 assembly statistics: *** Contiguity: Contig *** Total contig number: 162907 Total contig bases: 2734267806 bp Average contig length: 16784 bp Maximum contig length: 1051246 bp N50 contig length: 88741 bp N50 contig number: 7870 *** Contiguity: Supercontig *** Total supercontig number: 2205 Average supercontig length: 1240031 bp Maximum supercontig length: 126332868 bp N50 supercontig length: 45002363 bp N50 supercontig number: 19 Scaffolds > 1M: 147 Scaffold 250K--1M: 47 Scaffold 100K--250K: 34 Scaffold 10--100K: 473 Scaffold 5--10K: 235 Scaffold 2--5K: 434 Scaffold 0--2K: 835 ##Genome-Assembly-Data-START## Assembly Method :: ALLPATHS and Newbler v. 13-Feb-2013 Assembly Name :: Chlorocebus_sabeus 1.1 Genome Coverage :: 95x Sequencing Technology :: 454 Titanium; Illumina HiSeq; ABI ##Genome-Assembly-Data-END## FEATURES Location/Qualifiers source 1..16128 /organism="Chlorocebus sabaeus" /mol_type="genomic DNA" /submitter_seqid="Contig0.15654" /isolate="1994-021" /db_xref="taxon:60711" /sex="male" /dev_stage="adult" /geo_loc_name="USA: NC, Wake Forest University" /note="housed at the Wake Forest Primate Facility as part of the Vervet Research Colony" BASE COUNT 4064 a 4289 c 3978 g 3797 t ORIGIN 1 acgccaccca gccccggagc gcagccaggc ctgggggctg gaaggaaggg cgcaggaggc 61 ttgggacctg ggccccattc ctagggcaga cgcagatggg tgtggggagg aaggaaagaa 121 ctcattctcg cagctcgggg tgactcagtc ctggcctggc cgccctcagt gggttggggg 181 cgttgagagt ctctccccac aacgacctct gaccctggct gggccttctg ccagactggc 241 ccgggagttg gaaattttag aagcttcctc tgttagaagc gtctctgcag acggccgtgg 301 agcagcagag gctgagggta cccctagctt cccgggcccg cggagtactc cgcagccgca 361 tgcaccctgg caaggcggcc tccccgcagc cgaggtcgac gccaaagccc tgctctcctc 421 ctcccagggc gaggacccgc tccggcccta aggtggaaac cgcggctctc agcactggcc 481 agtcacctca gaaaaccctg ccaccaccac cactccgtgg ctcgttctgc ggactcagca 541 gccgaggcac cgccacttct gagacagaaa aggcgcgtag caggcggccc agacccaaag 601 cgacctcccc tccctgaccc ctgagcaccc caacctggtc cttcgaggcc tggagccgcc 661 taggcgctga gccactgccc gccgtgatct gtgcgactcc agatctgggc aggacccgca 721 gatcccatag gcccagcgat cgccaagccg cgacttcccg cttcctgcgg cgcctccagg 781 aaggcgagga ggacagcgga atgagtcctg gctgggctaa cctgggggac aatgaaactt 841 atattttaga aatattaaat caagacagat tcccaccact ctccgagtgg ctgatgggaa 901 cagttgtgaa tgtgggaaaa agaattaagc gtgccagagc cagcagcgga cctcctggcc 961 tgtaatgagc gcccaagacc ctcccccagc cggacccgca ctcgggaatc cggcaccacg 1021 gggaactctc caccctatcc ctgaagtctt ggtttcgttg ccagctctgc tcagcggcat 1081 caggcacagt gaaatgctct gttgctgatt gcagggtctg agaccggtga aagcgcagcc 1141 atagacgagc ccgggaggag tgggtaaggt ccaccgcatc cccactccag aggcgcagga 1201 atgtcccgga ggcccgagcc cccagaacct tccacgagcc catccttttg ttcacctggc 1261 cgggcctggc cagaggccca ctcggggcgt ctctcgaagc cggatcgtgg gtgaccccag 1321 cccaccccca cccggaggac cccgccccag tctcttgcca gaggccaagc cgacggtcag 1381 cagcaagtgt taatacccag tcaaagaggg gggctacttt gcgggaattt gtctccagga 1441 aaggatctaa gccttcttca atcggagcat atgttcatag agcaataaac cggaaagatt 1501 tacagtcctc gcccggcccc caacagtctc gcaccctttc aggaattact tacgatgatt 1561 tatcacacca acccgggcaa ttgtcacact gataaaatag cccgggccgg tgcccgccaa 1621 gccggggcct tgggagcctg ttggctgccg ggctgacagc agggctgggg aagctttttg 1681 tgggttcctt cctgggtcta caaggggata tctaggtggg ttggagtgaa tgagggtggg 1741 agaggagacg attctcattg agaaaaatgc aagcatctca gagaaagcac ccagaatctg 1801 cgcccgagtc actcgcaaag cacataaaca tttgtcatct ttgaataatt gaattaatgt 1861 gttattgttt gaatgtataa ttcataacgg gggaaacttt gtttctgtcc tgacggggaa 1921 atatacacac accaaaaaag aaatgaaaac acacacacac acacacacac acacacacac 1981 aattccaaaa caaccaccac gcgcagccag cccctgctcg ctgaaatgat tctgaaagcc 2041 aatggaggca tctcacagca gcaagaggga aggaggcaaa tgaagaggcg cccgggtcgg 2101 agctgcaagc tgaaagaaaa gaatgcccgc tgctgcccca gcccaggtcc ctggcaagct 2161 gggggtgggg tgggacggag cggcgtggct cttaggacac ccaattttct tgcagtgccg 2221 ttgaaaaaag gcagaaacgg gagcgaacag agaaacagcc ttctgcagcc cgccctggcc 2281 tctcccccat catcctttcc ccaggcttcc agtcgccccg gggtctccca gtcgggctgc 2341 agggactttt cctcgcgttt attttagctc ttccaaaata gaaataaaaa attcaaacac 2401 aaaaacaaag gggaaagaag aggaaactcg ctggggttac agaggcagca gagcttgggt 2461 ggggagagag gagccgggga gaagcaactc ggaaatcatt atctgttggt taaaatctgc 2521 ctggggtaaa atttcaactc gattttatag ccttctatta tgacgcaaag aaaaatttac 2581 gaccctcgct tgaaaagagg gccagggcct tttctcactg gtttaagaat aggcggcaga 2641 tgccgtgaca gcgaccattg ttcccggtag acacaggcga ccgggcaacc tcagcccgcg 2701 tgcgccaggc gcgtccccca gccgggccgg ttttttacaa ctcctgttgt cttggagcag 2761 cccgcttgca cctcgcgcct cacccctcgc cccctggcac cctagcacct cgcacacaca 2821 cccactccct gtttctgtct gtctctccct ctctcacaca cacaagcgcg cgcgcggacg 2881 cacgcacact cacacacaca ctcggccgct ctcagtaatt tttcataagt gcaaagcttt 2941 gctgaaccaa aggtcaccat ccaagccact aacaacttct cgccggcgcg cagttccaaa 3001 tcaggaagca aagggaacta tttgtcagcg gccgtgacag ctaagttcat taaggattta 3061 aatctgatga aataaaagtt gccaaaatat ttacctgcgg ccgcgctccc gcgcccgccg 3121 ggggccgggg gtggagggcc gggggcaccg cgcggccccc ctgggcgggt gatattcttg 3181 ggatccctgc actttccaag tcggagagag ccctttgcgt ggcagattaa tgacgactcc 3241 ttgtttctta agagacgtgc tgaattaatt atttcgccaa tcacgtggtc gggacttgta 3301 gaaatctatt cttatcatct tcctcgcact tagaaaattc tccgggttat gaaaggccct 3361 cccccggctg ccagcgagcc cccggccgcc caacccggcg cggggggcag ggagtgctcc 3421 ccaacatggc gggcaggcgg gcgggaagcg ccgtctgccg agggtcccga tttactgtac 3481 tgtatactca gcgggctgtg ctatggacgc gcttcctcct catccctgcc tgcgattcaa 3541 tacacacagc gttattgatg tattggtggg cacgggagga gccattaacc agaagacggg 3601 cagaggacaa tcataaaatg tgtctgaata tttaaaactt ctgcctgtgc acttataaat 3661 acacatgtcc acgcgtctgg ggcgagccct tccatgtatt aaggacacat gtctgatttt 3721 atgcattcta ctcatttcct gtctggaaca agcaccccat ctattttggg actcctgtct 3781 tggccccttc ttcaaggcga ggccaagatg tgaagaagcc cttgcggtaa atgtagtgca 3841 gggcagtttt tatagtgggg agtgtttatt gttgcccgag ggatggaggg ggcgcgggag 3901 aaggaggggc aggtgcagga cctgagcggc accggaaaag tagaagccca agagtggagg 3961 ccagggatca ggtcagagca gccacccagg agcctcaggt cagcccagaa cccagagcac 4021 tctccctgca ttggggatgg aggaccagta aggtgtctca tccaccactt cacaaagact 4081 tcctctaagg gcttccagac caaaaggggc tgggggctgg cccgggccca gcagaatgcc 4141 aatctggatc cccttattaa cctgggcagt tctttttctt caaaaaatta acacccaaca 4201 ctctgcaccc tgaattcaac cccagacttc ccaatgctca caaaatatga gggccaattg 4261 ggagtatgtg tatatgaggg agggggtaat aatagaattg ctaagcctta catggtttaa 4321 ccttcacatt ttaggcaccc cccaaaaaat ggtgcctaat ttattgcaga aaataataaa 4381 ttgtttatgt taagtgttag cttttagaag acttaacaaa gaatgaatgc ttctgttaag 4441 gcaccaaaca ggagggagat tttgctacac tttttattat tttaaatata gatcaatgaa 4501 ttacatcaaa actacaagca acaattagta taaataatac tttaatcagt ggcagcaaaa 4561 cattggtcaa ttctattaaa aaagcatctc gtgtgaacag acatcatagg ctgactgaca 4621 atgtcatctc ccaacaaaag gctgcgatgg acaaagtgag atgggagtca gaggagcaac 4681 ctcctcagca aacacttcac tctccccctc cccctccccc ggtcaggacc ccaacacggt 4741 tttggttttt gtttttaaaa caccaacaca aacacttctg gctcatattt aaaggaacaa 4801 actggaaaca aataatagca aatgggcact ggaggctttc cacacctaat tttctccaaa 4861 gcacgttctc agatggaaac ccatttcctt ccctgcccta agtggttgcc gggtcctacc 4921 ccttccctgg cccactcctg tgggcccaaa tggatgctgg ggtccggctg ggacctcagg 4981 gttgggggct gcactctggg ccatccaggc tccattaaca tttcaaaaga atcccttgaa 5041 gctgagaagg agagctgaga cttataaaca aagaagttgt ttctctgatt aagttacttt 5101 ccaaagtagt ggggctacgt ttgagaagaa gcttgggttt tccttatgaa aacatgtttc 5161 ttggctttcc tctggggcag ggatgaagtc agttaaactg ttgtggcatt tggtatgaag 5221 ctgggggtga gcaacctgaa cttctgaggt gggggaaaat ctatataaca aatctctatt 5281 atatatatgt agttggatgt tgtgaaaata tatacctttt tccgtttccc aaggagaagg 5341 ttcaatgtat acacagattt gagttttagg aaagcagctt agcaactttc caatttgcct 5401 aaggatagcg gctcctttca aatggtacat ccaaagactg ctctccatat tagagaggaa 5461 aaaagaagag aagggtagtt agagtccaaa aggttaaggg tttagaggtc agtcctcccg 5521 gctgaaatac agctaatcga atagaaaatt tgtcctctgg atgaacctga ttctgtaatt 5581 tacccggact tcagtaaaat accttttcag cttctcaacg tgagggcgtc agaaaaaaag 5641 atgtcttgac tatgtatgaa ctctgtgctt tttgtcatag aaacaatgat gtgaataatg 5701 caggaatatc catctttaat atcccgacat ggtgatattc aagtattgcc atgtgcaagt 5761 ctgagagcca ggctcacccc aaggaggaac aaagagcagt ccccagggtc taatacaaaa 5821 gaccaaggct tggaagacac tttagagaag cagaatttca gcctagagac tgcagcgaat 5881 acctgccctc agctctcagg agacacagcc aaagaacacg cctacagaga aactcttttt 5941 tccctcccac ttacatatat tttttaaatt accacagaaa ccctctaaag tgtttttgaa 6001 gtactgaaaa aaataccccc ttctggattt taaaattaag ttttaagctt atcctttctc 6061 cctttcccac cttggtcact ttttgatagc tacaacattt tttggagcct gaggccttgg 6121 gaagactggt ttcatctcag tatatgacct ctggtcatta attttgactt tttcctcctt 6181 atgcttgagg gtcagcattt tagaaaaatg aactaaaaga catctcccct gttgagagtg 6241 attccttaaa aaaccactat tctcaaggca aagatgagtg cttgaaaact tttatacttt 6301 tacaaattct gcttctaatt aatgagctga ggaaaacttc cttagatttt gttttttaaa 6361 aaattaaaac ttcaagggga aaaaacctct aaggctaact tttgtggctt tctagattca 6421 attagggttt tgtttttgtt tttccttctt aaagcaagag ttcctcacct tcccactttc 6481 tccccaccgc acagcccctc ccctacacag gccatatagt aattcctgta aaacattgct 6541 atatgggggt atttagggca tttggaatcc agttggaaca gaaaattcag tcattatcta 6601 accacccagt gcatagaggt tttgttttct ttccccagaa ggaaaatgtt ggaccatttg 6661 ttgctttttt tccctttcct ttaatcaccc agaactggtt gtaagcgcag tgctgaaaaa 6721 aaaacatgtg gaaatatatt ttcaagaaca caactttgag tgaatccttt gcttccttct 6781 ggaagggtta agggcccccc acacacacac atttccagag agtggggagg ggtggagttc 6841 agaagtaagt gcacatatct ctgattctgc tacctgaagt tcagcccctt tcaccttttt 6901 aagatttttg gcatgaaggt gtcagttatt tctgtaccac atttcccaac aaataaatat 6961 ttaagcaaat atctaaacat aaaataaccc gtttttactt ccttcttaga cccttttctt 7021 tggacatggg gccactggga gcaaaatata tatattttta atcacacgaa attccataga 7081 caacccagaa agttcagaaa tcctgaccca gctctccggc ttacactgaa agcacctagc 7141 acaggggtgg ccaaccttct gcctcaactc ccccacatta ttaggccaca aagctccctg 7201 gaggggtgga gtgggggaga atgtccaaat tcacactggg atacagcatc catccttcat 7261 ttgcaaattc aaaatccaag agcggtgatt ttcatgagtt taatgactcg ccaatatttt 7321 atgttctcag tagctcctgc catataatag agagagaatc ccgccccccc cactcccatc 7381 ccgctctagg gagggggctg ccctcgggaa ggatgctaac ctagtccgag ggcggcctgc 7441 ttgtcccagg tccaccccac agtaccttag agcccagcga ggtgacagca ggcccggcct 7501 gcccccgcag cttcggctca gcctggtgcg cagggagaga tgggcccagg gtccccactt 7561 ctataaggtc gtcaggtccg tgtggtggtc tttggccaag ggctgtaaat gctggccggc 7621 ggtgactgag gaggagcaag atgaggagga agatgaggac gatgacctgc ctttggtgtt 7681 gggcagctta tgatcttttt tccacttcat cctccggttc tggaaccaga tcttgatctg 7741 gcgctccgac agacacaggg tatgagcgat ttcaatccga cggcgccttg tcagatacct 7801 gttaaaatga aattcttttt ccagttctag gacttgctgc cgggtgtagg ccgtccggga 7861 ccgcttgggt tccccaccgg tgtagttggg gttcactgcg agacaaaaca gacaggacag 7921 gcaggtcaga gcctggccac tagttaggcc cctcgcctcc gccgctcact tcctggcgcg 7981 ccccgaggcc cgcggccctc cccgcgctcc tgagctcgct tgattcctcc ctcccgccct 8041 ctccccagcg cacacgctcc ctcccaccca cctcctcaaa cacacccaca taccccaggc 8101 ttgcgggatc cccagatgtg ctccttcttc cgggcccacc accaggtccg gaaccctcaa 8161 gcattttgga aatcccccgc tcctccacag ccttccccct gctctgaaaa tggagtcctc 8221 ccccacccca cccccatccc cacccccttt tgcgcccaca gccgagcagc cagccacatg 8281 gtcctgacct gctccctcag ctataaaaat ttatggggag agaaattgtg gcacccacgg 8341 aagctacaca cgcccccacc tacacgcaac cccttccagg cgacccaggc caacgggctg 8401 ggatgtagat agagaggttc ctggatccga gccttacccg aattcacgtg caccttcttc 8461 atccatgggt agaccacagc cggctgcttg agtgccgtcc cggggggcgg ctgcttgggg 8521 tcggactgac tgcaggcccg ggcgccaggc aggggcgccg gcggaggcgc cgggggcgct 8581 gggcaaggct ctcccggggc ggtgtagtga ccaccggggc cgtttggctc ttgtccgtga 8641 ctccgcgcag gcagcgccga gccagtcccg gggccgccgc ctccgaaagg ctgctcactg 8701 aagtcgggcc gtgggtagag ccccgggggc tggaagtctg cgccctgtgc accgccgccg 8761 tagtagtcgg cgccctggtc gcctaggtag ccgccctgca aatactcctc gcacggaggg 8821 aacttggggt ccacatactt ggagttcacc atatacgaac tcatgaccat taatttcaga 8881 aggtagaaaa tactaatttt tctcgtgttg tctttttttc tccttccata gggccctcct 8941 acttgctgtc aactgaataa agttgggcag ccatgtgacc aggccagcca atggcgaggg 9001 agcaagttta tcaccgggtt ggtgagcatc tcataatttt cacaaattta actaaataac 9061 atacgtataa tcaagtagct agctgtagca catttgagag ccctgccaga tattaatgta 9121 gttccagtcc ccagcacccc attcccctca ggtaatccca gctttgtctg ccactgattt 9181 tgccaacctt ccgtacccct tccactcacc cccagctgaa tgcaaccccc ccccacacca 9241 agtaggcaca gctcaaggaa gaatgaatcc tcctattggg gtacagagca ataaaaggtg 9301 ggtgggtgaa gtcgcctttg agttgggcag agtgccccag gcagggaggg ttcctgcctg 9361 cctgccctgc ctgcagccgg cgggagcggg gactctgcca tacaaaggcc gccgtcctgg 9421 ctttgttttc atcagggtca cccaaaagtt accagaatct ggccagcttg aatcaaatta 9481 ggcaatgagt aaccgccatc ctttctacca gtgcccaggc ccaggggttc ccagccaaag 9541 ctgggccctg ccaaaatagc agggggccct cgctgccctt gcccatgtag ctgggggtgg 9601 gctgggatgg tggcaagggc acccccacct tgccaaattg caccctagag ctccccactg 9661 ggcttcttcg aagcttgctt tcttcccaca ggagggcccc aaatacaagc aagcccctat 9721 ttgggtacct ttgcacctca tgttcctgga gggcagagct gggactaggc aagctggcct 9781 tgcacctcac tccctagtac cccaaaacct agccctttgg ccctgcatcc atgcaaccaa 9841 ttaggcttgc ttttctcttt tcttttcttt tcttttcttt ccttttcttt ctctttctct 9901 ttcagtcttt tcagcaccca gaggatcctc tgtttaaagg tgaggagctt ccggagagga 9961 aaaggctgct ctatgtcact aggcgcagct gatcctcctg gagtccaagg taataaaaca 10021 gaggaggctg accagcgccg gctgcttcta gtctccactt cgcacttcgg tctctggcta 10081 ttccgaagaa acgtgaagtt tttgcatcga ccatatattc ccctagaatc gaatctgtga 10141 ctatacggat accacacaaa ttcggttcta cagggtatat atagacaacg ttacaacctc 10201 tggtcccacc cagagtgagg tacctaggct gctgccggcc ccagcctctg cctctgagcc 10261 accaccgttg gacgcagcca cccgcacttt ctgaaacagt tcaggtagcc tgatgggctt 10321 ggcgacaatt tgaagcaatg agtttcctca ttcttcggcc agggtcaggg ttaccaagaa 10381 cgggatcaac cagaatattc ttcttaagaa agaaaaaaag aaagaaagaa agaaaaataa 10441 tccatcccaa ggatagcagc tttattaacc ccctccccca ggatccaaat accgcccccc 10501 tccttatcaa gtggtatttt ccagaaaaga acaaatgtcc ttggcggcag aagaaaaaga 10561 agcgagaggg gaatatcatc tgtccactcc ttttcctact gcctgaccaa gccaggttgt 10621 ctgccaaagc tacttctcca agaagtcgca catttacctt catgggtcct gatccgggct 10681 ggcctctccg ttccgctgct ggctgctgct gcagacccca aggcaggcgc tgctcacagg 10741 caccgagccg ccttgggctg aggccggcgc gctaatggtg aagggcaggg cgaggggtca 10801 ttcgaaatca tttacaaaca taccacaaac tttcattatt tttgctttgc cgacagcagc 10861 acacaagagt acaaaagttc acaaatttca cccgccccag cgcctcggca ctgtgcagtg 10921 cttgctcctc agttcagggc atggagagcg cagactcact cagagcctgg atggatttca 10981 ggggaacagg gatgccctgg ctcgaatggg gcttttgggt gcctaactgt aggctgaagg 11041 tattagtttt ttgttttttg ttttttactg tcttgattgc ccttcctacc tccaacataa 11101 ttcaaaaatt agtttcaagc ctaagcctgg gcctgggcct ggtggggtgg ggcagggata 11161 cttccccttc aagcctggca ctacatcccc ccatcctttg cttccaccac tttacctatt 11221 tcctagggat ttttggggtg ccggttctaa caagggctat gaggctgcag aatctgttta 11281 tcccgggaga gtccccccag tttcccggaa tgccaaggac tcccaagtat ccaccttcct 11341 gggacactgg gcctaggctc tatccagaca aattgaggga gcctgctgag ccggctgggg 11401 actggggtga atgtacacat tttcatattt gttagacgct gtgacctgca tttcacctta 11461 ttgctcgact tgttcagaca ggtcaaagcc aacgagttat tgatgaacaa aagcgttaaa 11521 tattcacctc ccgctcaact gcccatacta aatgcttttg atctctcggg ctttttgtgc 11581 tgtgttcagc tacagggcgc cgaatgtggg tttgctagcg agcaggttct gcagagataa 11641 gcacccagag ccagtggagg ggaggagaga agcaagacca ggaggtcccc aggacatcag 11701 attttccaga ctggagccca gagccccttt cacttgagcc agcctggtgt ataggggtaa 11761 ctgctcttct aggggtgtgt acagcctacg aagggtcgac tagactgccc agtccagcgg 11821 ggccaaaaaa cctgtctctg agtccagccg tagcttttcc aaccatgaag gagggagccc 11881 ggttcctgca gccctcgcat attccaactc ccattaaaat aaaatcacct tcgttttgca 11941 gtgagggagg ggccacagtt ctttctgtga ctctccctcc aataccattg tcttaatgag 12001 atggtcctcc aggaggcttc acaggcctaa tcagaaatgt acctgtcttt cgcggcacag 12061 gcagctcaga aagatccaaa catccaagcc aaggacccca cgagtctctg gccccacccc 12121 tccgcaccca acccactcca ccgtcctatg cctccttgca gcaccagcgc ctctggggca 12181 gaaattctcc agctgcagtt acagagaagc agaggtgaaa agaaggtccg tatccctcct 12241 tgctcagcca gtgtcccaag cccagggccc acaccgctgc aagcagaacg ggggaggggc 12301 ggggaaggaa tgtccacgta aagctcagaa gcgggtagct caacacaccc atgccataga 12361 ggattgagtt tttgttttca gtgaattttt ttaaaaacag aaggttggcc aggctagaac 12421 aggttaaggg ttgggctgcg gaggggattg cattgccttg gtgtttactg cgttggtttg 12481 atgccgctaa taagacgggc tgctaattag caagctctta acagaagcga gcgccttggc 12541 ggagctgcac ctgtgcagag cagggaaagg ccgcgaaggt ccaggaaggc tcaaggagac 12601 aagggcgaga ccccgcggac cgttgcctca ggcaggtgtc tgtgaaccag gccccagaag 12661 agctttccag gacctcgatt tagcgcctcc acctcccgaa gcggactgac ctcgcagagc 12721 gaggccgggg atggaaggtg gggtgggggt ggggtggtgg aggcagggcc tttcttccaa 12781 atccgcccac tccactggct cggcccttag cccaccactt ccccgcgatc agtaccgcct 12841 cttcgcgatt cggaagaagc gggaaggtac ttacagcggc gctctctcat ggtcctgaac 12901 ctgttgcaat gaaaacgggg atcacttaac ttccacagga taataaagca gaaacataac 12961 acacagaaaa atgacaaggc ccagataccc caccaatacc cagacctccc tgcttcaggt 13021 gcccgccccc gggaactgcg caaagttctc cgcggagccg aagaccgcag tcgctcaaga 13081 ggagagccgc gtctccatcg cctgccccac tccacgcacc cttgggcaaa acctcactgg 13141 ttacttggtc cgagtcaggt cgcccgtaac agggttttcc cagtgggaaa acaagaagcg 13201 aagagaaaat gggcctttgt gtgtttcctt ccgcgcaatc gggctgggtg ggtgcaggta 13261 cactggggag gggtagccgc tccatctgcc cctcacccca gcttcctatc tctcccaccc 13321 tgggagcaat tcagcctccc attccagctt caggttggtt tcaaattcct cagccccaac 13381 cagtgccccc caaacctgtc ttctcctcct caaagaagaa tcaggcatgg ctgggacttg 13441 ccacttctac ttcaattcaa aacaaagagc aatttccagg gggaaaaatg aggacccaaa 13501 tgataaccaa ggaggaggaa aatcaatgag tttaaaggtg aatggtaatg ctttagattg 13561 ttatccagct cttagggtgt gacatcaatt tttcaggatt gctgctgaga gcccagaaga 13621 caatcttact atgcagtggt cctcccagag gctggtgtcc agttttaagt tttcagtctt 13681 tcctgatatt attataggaa ggcaatatca gaaatctggg ggacaaagca tcttcaccct 13741 catcctctct ttctttcccc acccagaata ccactgtttt attccacttc tgaaggagat 13801 gctcttatgg ggaggagagg ggcaataagt ccttcctgtg aggtgagagc cctcaattcc 13861 taactgtccc tatccagaag ggagggcata ggacatcgag atgcttattc tatttgttaa 13921 tttaggttta ggctaaaatt agaattaact tcggtgagac ttatccccat cctaggcaga 13981 aaagtcagga atagagaaat ctcccacctc tgaagttgtt gtggaaaaag taataagtga 14041 cttactgttt ttctttccta gtgggcaggg gagctgggag tagaactgtc tccttcttag 14101 cccggcgctg gctggagcct ctcacgttcc tattgctgct ccaggaaccc agcatctgca 14161 cttgtatgct cttaacctaa tttatggtgg gaattatatt ttggcttgtc aactctcaag 14221 ccataaaaca aagtttattg gaatcacgtg ctcataaata gccactcagg tcccatctct 14281 acagaaccat aaataacctt tgactttaaa cttttattca ctaaataaag gtttgctgca 14341 actgtttcct cagatttaaa tagttccaaa tatccaagat gcaagggagt tcatatttat 14401 atcaataccc cagacttttc aaatagcatc ttttcagaga aactgtttaa gttgggagat 14461 agttctattt ggataaggct ggttggtggg catgtctcat gattctttgg ctgcattcac 14521 tgtgagtacc attaacccct ttatttcaat aattttctag ctctagatgt gatagagaaa 14581 ggtcaaaagc cacttcttat ttgcttatga cagaagttgg aagttatttg agattttgtt 14641 caagtttcaa tagagaaagg gtggttaggg agaggctcgg tgggtttggg ggtgggagtg 14701 gcagttggaa ttaacacttg gccccctcgg tgttgttgtg gatgggcttg gagaaagtcg 14761 aaggtagcca catgcctaga ggtttctata atttgcttag atctgaaagg gaagcctttg 14821 caagtgacca aactgcttaa aacagcaaga cttggggttt ggtaaagtga gaaagtggag 14881 aatagcctca ttagaatctt tggcaactaa aactccaaag aagtaaaaca ataaaacaat 14941 aaaacagccc tggtgtgaag gagaaagggt gtgaggaagc caatgagact ctcaaaccta 15001 ggaagtcaga tgtccccact cccatagtgt ctccaagcac agggattaag gctcaggagc 15061 tttgggggct caaaactgcg tgggagtttt aggaggccta cctccacacc cacactgctg 15121 cccccaccag ggagagagcc taagagagga agagggagta gtacactgcc gctgtcctgc 15181 tgagaaaacc aaggaaacag cttgggcctg aagctactct ctaggagaat gtggcattct 15241 ctgcttgggc gaggctgggg tgggggtaag agagagggaa agatgccctc agctcccacc 15301 aaggagcata aataaaaaga gaactgaccc cctagcaccc ttcaatagcc caccagagtt 15361 gccaccaaac agtgtaaaaa catgtgtggt tttgactatt ctgatgaaaa taatggatgt 15421 tctgtggaga tttgtagagc acacacacat atgatttcta aatcaaattc agttgcaact 15481 gttcccatca gaaagaccca tgaaacccat aaaagagatc ccttcataca aagatctctt 15541 tgcatcccaa tttccaccca ttctacatgc atttccaaac ccatttcctg atttgactgt 15601 cattagctag aaagcagggg gttattagcc tggattgtaa ggcatccatt tctccttttt 15661 ctgtttcatt agccatgtag aaagatattt ttcttttatg gttgatggca tctgttttta 15721 aaaatggata aactcttcaa aacatagttt ctgattctgg ttagcaccag atgagcagct 15781 gtaaaataat aataataata gtttgagggg ttgagaagag ctatctttat tttcctgtaa 15841 ccccagcact ttgggagact gaggcaggca gatcacaagg tcaggagttt gagaccagcc 15901 agaccaacat tgtgaaaccc catctctact aaaaatacaa aaattagttg ggcgtggtgg 15961 tgcacctgaa atcccagcta ctcaggaggc tgaggcagga gaatcgcttg aacccaggag 16021 gcagatgttg cagtaagcca agatcgcacc actgcactcc agcttgggca acagagctag 16081 attctgtcag gagagaagga aggaaggaag gaaggaagga agaaggaa //