LOCUS       AJ583866                 310 bp    mRNA    linear   VRT 08-DEC-2003
DEFINITION  Rana temporaria mRNA for Ranacyclin T (rncT gene).
ACCESSION   AJ583866
VERSION     AJ583866.1
KEYWORDS    Ranacyclin T; rncT gene.
SOURCE      Rana temporaria (common frog)
  ORGANISM  Rana temporaria
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Amphibia; Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Rana;
            Rana.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Barra D.
  JOURNAL   Submitted (29-SEP-2003) to the INSDC. Barra D., Dipartimento di
            Scienze Biochimiche, Universita La Sapienza, Piazzale Aldo Moro 5,
            00185, ITALY.
REFERENCE   2
  AUTHORS   Mangoni M.L., Papo N., Mignogna G., Andreu D., Shai Y., Barra D.,
            Simmaco M.
  TITLE     Ranacyclins, a new family of short cyclic antimicrobial peptides:
            biological function, mode of action, and parameters involved in
            target specificity
  JOURNAL   Biochemistry 42(47), 14023-14035(2003).
   PUBMED   14636071
  REMARK    Biochemistry DOI: 10.1021/bi034521l
COMMENT     Related sequence: P83663
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Rana temporaria"
                     /mol_type="mRNA"
                     /geo_loc_name="Austria"
                     /clone="Rt-26"
                     /tissue_type="skin"
                     /db_xref="taxon:8407"
     CDS             40..228
                     /gene="rncT"
                     /product="Ranacyclin T precursor"
                     /function="antimicrobial"
                     /db_xref="GOA:P83719"
                     /db_xref="InterPro:IPR004275"
                     /db_xref="InterPro:IPR032019"
                     /db_xref="UniProtKB/Swiss-Prot:P83719"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /protein_id="CAE48162.1"
                     /translation="MFTMKKTLLVLFFLGVVSLSLCVEERDADEEDGGEVMEEEVKRG
                     ALRGCWTKSYPPKPCKGK"
     sig_peptide     40..105
                     /gene="rncT"
     mat_peptide     169..219
                     /gene="rncT"
                     /product="Ranacyclin T"
                     /note="Not detected in skin secretion, possibly because of
                     its scarce amount. A similar peptide, ranacyclin E, was
                     isolated from Rana esculenta skin secretion"
                     /experiment="experimental evidence, no additional details
                     recorded"
     polyA_site      289
                     /gene="rncT"
BASE COUNT          109 a           54 c           72 g           75 t
ORIGIN      
        1 ctacattctc agcaccaact gaactacccg agcccaaaga tgttcaccat gaagaaaacc
       61 ctgttagtcc ttttctttct tggggtcgtc tccttatctc tctgtgtgga agagagagat
      121 gccgatgaag aagatggagg ggaagttatg gaggaagaag taaaaagagg cgcactcagg
      181 ggttgctgga ccaaaagtta tccaccaaag ccttgtaagg gaaaatgatg aaacatgatg
      241 aggaagtcat ctgatgtgga atatcatata gctaaatgct aaatgtctaa taaaaaaata
      301 aaaatattaa
//