LOCUS AJ276513 10146 bp DNA linear BCT 15-APR-2005 DEFINITION Ralstonia sp. CH34 plasmid pMOL28 cobalt-nickel-resistance system (cnrY, cnrX, cnrH, cnrC, cnrB, cnrA and cnrT genes) and tnpA gene (partial). ACCESSION AJ276513 VERSION AJ276513.1 KEYWORDS cnrA gene; CnrA protein; cnrB gene; CnrB protein; cnrC gene; CnrC protein; cnrH gene; CnrH protein; cnrT gene; CnrT protein; cnrX gene; CnrX protein; cnrY gene; CnrY protein; nickel transport; tnpA gene; transposase. SOURCE Cupriavidus metallidurans ORGANISM Cupriavidus metallidurans Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Cupriavidus. REFERENCE 1 (bases 1 to 10146) AUTHORS Nies D.H. JOURNAL Submitted (20-MAR-2000) to the INSDC. Nies D.H., Martin-Luther-University Halle-Wittenberg, Inst. Microbiology, Kurt-Mothes-Str. 3, 06099 Halle, GERMANY. REFERENCE 2 AUTHORS Grass G., Grosse C., Nies D.H. TITLE Regulation of the cnr cobalt and nickel resistance determinant from Ralstonia sp. strain CH34 JOURNAL J. Bacteriol. 182(5), 1390-1398(2000). PUBMED 10671463 REFERENCE 3 AUTHORS Grass G., Kuehnemund S., Fricke B., Sutter K., Menge S., Krauss G.J., Nies D.H. TITLE Signal transduction pathway involved in regulation of the cnrcobalt/nickel resistance determinant from Ralstoniametallidurans CH34 JOURNAL Unpublished. COMMENT Related sequence M91650 FEATURES Location/Qualifiers source 1..10146 /organism="Cupriavidus metallidurans" /plasmid="pMOL28" /strain="CH34" /mol_type="genomic DNA" /note="previously annotated as Alcaligenes eutrophus CH34, provisional name Ralstonia metallidurans" /db_xref="taxon:119219" regulatory 924..933 /gene="cnrYp" /citation=[2] /experiment="experimental evidence, no additional details recorded" /regulatory_class="minus_35_signal" regulatory 946..952 /gene="cnrYp" /citation=[2] /experiment="experimental evidence, no additional details recorded" /regulatory_class="minus_10_signal" regulatory 959 /gene="cnrYp" /note="promoter +1" /citation=[2] /experiment="experimental evidence, no additional details recorded" /regulatory_class="promoter" CDS 983..1270 /transl_table=11 /gene="cnrY" /product="CnrY protein" /function="transmembrane signal transducer" /note="part of the CnrYX anti sigma factor complex for the ECF sigma factor CnrH" /db_xref="GOA:P56621" /db_xref="InterPro:IPR035230" /db_xref="PDB:4CXF" /db_xref="UniProtKB/Swiss-Prot:P56621" /citation=[3] /experiment="experimental evidence, no additional details recorded" /protein_id="CAB82448.1" /translation="MADVEEWLTHARKVTQEASIGVDVTSIQECISAEPAQRVLVARR DAWRAICCAAFAALVAFAAINRVATIMLEKPAPTWVATPSAASPFGLLIGK" CDS 1267..1713 /transl_table=11 /gene="cnrX" /product="CnrX protein" /function="periplasmic nickel sensor" /note="part of the CnrYX anti sigma factor controlling CnrH; hydrophobic amino terminus of the protein is not cleaved off" /db_xref="GOA:P37975" /db_xref="InterPro:IPR025961" /db_xref="PDB:2Y39" /db_xref="PDB:2Y3B" /db_xref="PDB:2Y3D" /db_xref="PDB:2Y3G" /db_xref="PDB:2Y3H" /db_xref="PDB:3EPV" /db_xref="PDB:3ZG1" /db_xref="PDB:4WWB" /db_xref="PDB:4WWD" /db_xref="PDB:4WWF" /db_xref="UniProtKB/Swiss-Prot:P37975" /citation=[3] /experiment="experimental evidence, no additional details recorded" /protein_id="CAB82449.1" /translation="MMKSRTRRLSLSTLFGALLGVSVAAAWLYYSHRNEAGHGDLHEI LHEAVPLDANEREILELKEDAFAQRRREIETRLRAANGKLADAIAKNPAWSPEVEAAT QEVERAAGDLQRATLVHVFEMRAGLKPEHRPAYVRVLIDALRRGSQ" CDS 1710..2285 /transl_table=11 /gene="cnrH" /product="CnrH protein" /function="ECF sigma factor" /note="extracellular function sigma-factor required for transcription initiation at promoters cnrYp and cnrCp" /db_xref="GOA:P37978" /db_xref="InterPro:IPR000838" /db_xref="InterPro:IPR007627" /db_xref="InterPro:IPR013249" /db_xref="InterPro:IPR013324" /db_xref="InterPro:IPR013325" /db_xref="InterPro:IPR014284" /db_xref="InterPro:IPR036388" /db_xref="InterPro:IPR039425" /db_xref="PDB:4CXF" /db_xref="UniProtKB/Swiss-Prot:P37978" /citation=[3] /protein_id="CAB82450.1" /translation="MNPEDADRILAAQAASGNQRAFGQLVARHGVALAQAARSFGIPE TDVDDVVQDTFVAAWHALDDFDPDRPFRAWLFRIGLNKMRDLYRFRRVRQFLFGAENL GDLELAGGVANDEPGPEQQVAARLELARVASTLGKLDTGSREVIVLTAIVGMSQPEAA AVLGLSVKAVEGRIGRARAKLSALLDADSEK" regulatory 2298..2307 /gene="cnrCp" /regulatory_class="minus_35_signal" regulatory 2320..2326 /gene="cnrCp" /regulatory_class="minus_10_signal" regulatory 2333 /gene="cnrCp" /note="promoter +1" /regulatory_class="promoter" CDS 2358..3614 /transl_table=11 /gene="cnrC" /product="CnrC protein" /function="outer membrane associated protein" /note="probably required for nickel export across the outer membrane" /db_xref="GOA:P37974" /db_xref="InterPro:IPR003423" /db_xref="UniProtKB/Swiss-Prot:P37974" /protein_id="CAB82451.1" /translation="MKQVISSFLCRPRFVGSAIWLLPVALSHAAEAPPFPNLLQQSLA LAPAMVAQAANVRAAGADAAQAQAWLNPRIDTVLENLGAPSSDGLSQRQNTYSITQPF ELGGKRGARIEVGERNFAAAQARERQAQVAYAAELAVAYATAEAALGRKILATENLAR ANEELAAARALVDSGKEASLRSAQAKASVAAAQAAEAAATNDATQALARLSAMSGASE PYTAVTSSLLTTQAVVPNAPAALAESPSVRAAEAERNALDAQVDVERKRWIPDVGVSA GVRRYGWTNSSGYVVGVTASIPLFDQNRNGINAAVERVAAAQARLDSVRLEANVARQS AISQVATADKQLAAASEGEQAAAEAYRMGRIGYESGKTPLMELLAVRRALVDARQLTI DARLARVRALAALAQADGRLAFEESR" CDS 3611..4798 /transl_table=11 /gene="cnrB" /product="CnrB protein" /function="membrane fusion protein" /note="probably required for transport of nickel across the periplasmic space" /db_xref="GOA:P37973" /db_xref="InterPro:IPR005695" /db_xref="InterPro:IPR006143" /db_xref="InterPro:IPR032317" /db_xref="UniProtKB/Swiss-Prot:P37973" /protein_id="CAB82452.1" /translation="MMKNERRSVNWPMIAGVAAVAAAVGFGAAHLPVSEKSPASTQAP EAQKPQSAPVKPGLKEVKIPATYLAAANIAVEPVASAAVGTEILAPATVAALPGSEAV IVSRAAGAVQRVQRRLGDVVKAGDVLALVDSPEAAGMAAERKVAQAKADLARKTYERE ASLFQQGVTPRQEMEAAKAALDVAQAEALRAATVAQSAHLASDGRSVAVVSPIAGKIT AQSVTLGAFVAPQAELFRVAGTGAVQVEAAVTAADTSRIVAGSEATILLANGSPLSAR VQAVTPTVTGSARVATVVVVPAQPTDRLVVGEGVQVRLRTAVADAAALSVPEDAVQNL DGRDVLFVRTQEGFRPMPVLVGTRSGGSAQILSGVQAGEQVATRNAFLVKAEMNKGGG DEE" CDS 4795..8022 /transl_table=11 /gene="cnrA" /product="CnrA protein" /function="RND protein" /note="proton/cation antiporter of the resistance-nodulation-cell division (RND) family, required for nickel export across the cytoplasmic membrane" /db_xref="GOA:Q7B054" /db_xref="InterPro:IPR001036" /db_xref="InterPro:IPR004763" /db_xref="InterPro:IPR027463" /db_xref="UniProtKB/TrEMBL:Q7B054" /protein_id="CAB82453.1" /translation="MIESILSGSVRYRWLVLFLTAVVAVIGAWQLNLLPIDVTPDITN KQVQINSVVPTMSPVEVEKRVTYPIETAIAGLNGVESTRSMSRNGFSQVTVIFKESAN LYFMRHEVSERLAQARPNLPENVEPQMGPVSTGLGEVFHYSVEYQYPDGTGASIKDGE PGWQSDGSFLTERGERLDDRVSRLAYLRTVQDWIIRPQLRTTPGVADVDSLGGYVKQF VVEPDTGKMAAYGVSYADLARALEDTNLSVGANFIRRSGESYLVRADARIKSADEISR AVIAHGKMSHHVGQVARVKIGGELRSGAASRNGNETVVGSALMLVGANSRTVAQAVGD KLEQISKTLPPGVVIVPTLNRSQLVIATIETVAKNLIEGALLVVAILFALLGNWRAAT IAALVIPLSLLVSAIGMNQFHISGNLMSLGALDFGLIIDGAVIIVENSLRRLAERQHR EGRLLTLDDRLQEVVQSSREMVRPTVYGQLVIFMVFLPSLTFQGVEGKMFSPMVITLM LALASAFVLSLTFVPAMVAVMLRKKVAETEVRVIVATKESYRPWLEHAVARPMPFIGA GIATVAVATVAFTFVGREFMPTLDELNLNLSSVRIPSTSIDQSVAIDLPLERAVLSLP EVQTVYSKAGTASLAADPMPPNASDNYIILKPKSEWPEGVTTKEQVIERIREKTAPMV GNNYDVTQPIEMRFNELIGGVRSDVAVKVYGENLDELAATAQRIAAVLKKTPGATDVR VPLTSGFPTFDIVFDRAAIARYGLTVKEVADTISTAMAGRPAGQIFDGDRRFDIVIRL PGEQRENLDVLGALPVMLPLSEGQARASVPLRQLVQFRFTQGLNEVSRDNGKRRVYVE ANVGGRDLGSFVDDAAARIAKEVKLPPGMYIEWGGQFQNLQAATKRLAIIVPLCFILI AATLYMAIGSAALTATVLTASPLALAGGVFALLLRGIPFSISAAVGFIAVSGVAVLNG LVLISAIRKRLDDGMAPDAAVIEGAMERVRPVLMTALVASLGFVPMAIATGTGAEVQK PLATVVIGGLVTATVLTLFVLPALCGIVLKRRTAGRPEAQAALEA" CDS 8068..9123 /transl_table=11 /gene="cnrT" /product="CnrT protein" /function="nickel transport protein" /db_xref="GOA:Q9L3G0" /db_xref="InterPro:IPR000620" /db_xref="UniProtKB/TrEMBL:Q9L3G0" /protein_id="CAB82454.1" /translation="MSIRTASPALLAALLFGASTPLAKALTGSVAPLLLAGLLYLGSG IGLALVLAIRRAWITPDPGNDDVGIPRGEVPWLIGAILAGGVAGPALLMTGLVSTNAA SASLLLNVEGVFTAVIAWVVFKENADRQIVLGMIAIVAGGLLLSWQPGSLQMSTGALL IVGACLCWAVDNNLTRKVSSNDALLVACLKGLVAGLCNTTLALTHGAVFPSVSTAAAA LIIGFAGYGVSLALFVVALRLLGTARTGAYFSVAPLFGVLLAFAIWPEVPGFAFWAAL ALMALGVWLHLRERHEHEHTHEPLEHSHSHRHDEHHQHEHDFPWDGKEPHRHWHRHEV MVHKHPHYPDVHHRHSH" CDS complement(9214..>10146) /codon_start=1 /transl_table=11 /gene="tnpA" /product="transposase" /db_xref="GOA:Q9L3F9" /db_xref="InterPro:IPR002513" /db_xref="UniProtKB/TrEMBL:Q9L3F9" /protein_id="CAB82455.1" /translation="LETTRSVWQARTDPRRRTASIGMYTHVRDRWGIFYDQPILLNER QAGAAIEGVIRQNGGEDVTQLAVDTHGYTDFAMSLSRLLGFDLCPRLSHLRDRRLHVP KGYAVPSELAAVADADVRLVLIEIAWDELVRIAASVQTGQCTAVQALTRFGAAARGQH VYEAGVHLGRLFRTIFLVDYFTNSAFRQEMQHALNRGEAVHTVQRAIHYGKIPLELAR HDASLAAVSSSLTLLTNAVMAWNTLHMQHALEAIEALGGESMRAEDLRQIAPTRLEGI NLRGTFDFPIARYAHRLLPSATTAQTLATTWRTG" BASE COUNT 1902 a 3000 c 3245 g 1999 t ORIGIN 1 gaattcatcg ccccctgcgc atacgacaga gcccctgtac atccgggcca tcgttcttgt 61 tgccgagttg ggtctattta ggaggtgcct tatgaaagga ctctcgatcg cattgattgt 121 gatggatatc tcgccgttcc ttctgctgtt gccgccgcgc cacgacaacg caagcgcgaa 181 ggcgattctg gttcggcgag actagggacg atgacgttgt acttcgctgg aaagagcagc 241 cccgatgcca aatcctgcga ggggaactgc gagcggaatt ttccacctgc actcgccgct 301 tccgacgaca aaccggttgg gatgctcacg ctagtccccg gccgcggtgg taaatcgcaa 361 tgggcttatc aaggaaagcc gttgtatcgc ggcctgatgg acaagaagcc aggcgaccgt 421 tcaggggatg gtctgaatga ggtttggcac agcgtgcctg tgcgatgaac gcttggtcga 481 aatgagagtt cgggcgggag accctcgtgc attgactgcg gtcagaaccc tggcggccgc 541 gacgttggcg gccgccaggt aatgcctggc tttgctttag tggcagtgac gaatgccggt 601 cttctggtcc atatgacagc cttgactgtc agttccgccg ctgtgcgccc atacactgcc 661 cgatgcgcaa gccagaacgg cggtgagaat gatggcaatc cgtttcaaag tcagtctccc 721 tggaatattg gtttgcggtc tcaatttgcc gcacgctcaa actatcatca gagcggtgat 781 taggccttcc ccgccacggg agggcctgtt gcggccgtgt cacagcgcca acgcccgaga 841 atctcggcgg caagacgtgc cgcgaggctg aagtctcctt cagcccctga gtcgcgaatt 901 tctgcaattt tctacccgag gttcaagggg gagtccttcg cgcagccgta taagaggcaa 961 tggatctgga tggaggtttg ccatggcaga cgtggaagag tggctgacgc acgcgagaaa 1021 agtgacgcaa gaggcgtcca ttggggtcga tgtgaccagc attcaggagt gcatcagtgc 1081 agagccggcc caaagagtcc tcgtcgcgcg ccgcgacgcg tggcgtgcca tctgctgcgc 1141 cgcatttgca gccttggtcg cgtttgcggc aatcaatcgc gtggcgacca tcatgttgga 1201 aaagcctgcc ccgacgtggg tggcgacgcc gtccgccgcc tctccctttg gactcctcat 1261 cggtaaatga tgaaatctcg tacccgacgg ctttccttgt ctacgctgtt tggcgcacta 1321 cttggcgtgt ccgtcgcggc ggcatggctg tattactcgc atcggaatga agccggacat 1381 ggtgacttgc atgaaatcct gcacgaagct gttccactag atgccaacga gcgcgagatt 1441 cttgagttga aagaagatgc ctttgcgcag cgtcggcgcg agatcgaaac acgcctgcgg 1501 gctgcaaatg gcaagcttgc cgacgccatc gccaagaatc ccgcctggtc accggaggtg 1561 gaagcagcaa cccaggaagt agagcgggcc gccggcgatc tccagcgagc gaccttggtt 1621 cacgtgtttg aaatgcgtgc gggcctgaag ccggaacatc gacctgctta cgtacgagtt 1681 cttatcgatg cactacgccg cggctcgcag tgaatccgga agacgctgac aggatccttg 1741 ccgcacaggc cgcttccggc aaccagcgtg cattcggcca acttgtggcg cgacatggcg 1801 tggcgctggc tcaggccgcg cgcagcttcg gcatccctga aaccgatgtg gacgacgtcg 1861 tccaggacac ctttgttgcc gcctggcacg ccctggatga cttcgatccc gacaggccat 1921 ttcgcgcctg gctcttccgc attgggctga acaagatgcg ggacttgtat cgctttcggc 1981 gcgtcaggca gttccttttc ggtgccgaaa acctcggcga cctggagctt gcgggcggcg 2041 tggccaacga cgagccgggc ccggagcaac aggttgccgc ccggctggag ttggcccgcg 2101 tggctagcac cttgggcaag ctggacacgg gttcgcgaga agtcatagtc ctgacggcca 2161 ttgtcgggat gtctcagcca gaagcggctg ccgtgcttgg gctgagtgtg aaggctgtcg 2221 aagggcgcat cgggcgggca cgggccaagc tttccgccct gctggatgct gactcggaaa 2281 aataagtttg gattcaccga ggggaagcat cgacctggtc cgtatagaga gggatagcct 2341 ccaccgctga cctcttgatg aaacaggtga tctcctcgtt cctttgtcga cctcgcttcg 2401 tcggctccgc aatatggctg ctgcccgttg cgttgagcca cgctgccgag gcgcctcctt 2461 ttccgaacct gttgcagcaa tcgcttgcac tcgctccggc gatggtcgcg caggccgcca 2521 atgttcgtgc ggctggcgcc gatgctgctc aggcccaagc ctggctgaat ccgcgaatcg 2581 atacggtgct tgaaaacctt ggcgctccca gtagtgatgg gctgagtcag cgccagaata 2641 cctattcgat cacgcagcct ttcgagctag gaggtaagcg cggcgcacgg atcgaagttg 2701 gcgaacgcaa cttcgctgcg gcgcaagcac gggagcgtca ggcacaggta gcctacgccg 2761 ccgaacttgc agtagcctat gcaacggctg aagcggcgct cggacgcaag atactggcca 2821 cggaaaacct ggcgcgtgcc aatgaagagt tggcggccgc gcgtgcgttg gtcgattccg 2881 ggaaagaagc cagcttgcgc agcgcgcagg ccaaggccag tgtggcggcg gcgcaggccg 2941 cggaggcggc agccaccaac gatgccaccc aggctctggc gagattgtcc gcgatgtccg 3001 gcgcctccga accctatacg gccgtcacga gttccttgct gacgacccag gccgtcgtcc 3061 cgaatgcccc ggcagcactc gccgagtctc cgtcagtgcg ggccgccgaa gcggaacgca 3121 atgcgctgga tgctcaagtt gacgtcgaga ggaagcgctg gattcctgat gttggcgtca 3181 gtgcgggcgt ccgccgctat ggctggacca attccagtgg ctatgtggtt ggggtcacgg 3241 cctccattcc attgttcgat cagaaccgga acggcatcaa tgccgcggtt gagcgggttg 3301 cagccgccca agcgcggctt gacagcgtcc ggctcgaggc caacgtggcg cgccaatcgg 3361 ccatatccca ggtagcgacc gccgacaagc agctcgctgc tgccagcgaa ggagagcagg 3421 cggcggcaga agcctatcgc atggggcgca ttggctatga atccggcaag acgccactga 3481 tggaattgct agcggtgcgg cgagcgctcg tcgacgcccg gcaactgacg atcgatgcgc 3541 gcctggcgcg cgtgcgcgcc ctggcggcgc tggcacaggc ggacggccgc ctagcctttg 3601 aggaatcacg atgatgaaga acgagcgccg gtcggtcaac tggccgatga tcgcaggggt 3661 cgccgccgtc gcagcggcag taggttttgg cgcggcgcat ctcccagtgt cggagaagtc 3721 ccccgcatct acacaggccc cggaagcaca gaagccacaa tccgccccag taaagcccgg 3781 tctgaaggag gttaagatcc cagcaaccta tcttgccgcc gcaaacattg ccgtagaacc 3841 tgtggcgagc gccgccgtcg gaacggaaat acttgcgccc gccacggttg ctgcgctgcc 3901 cggtagcgaa gccgtgatcg tatcgcgtgc ggccggcgcc gtacaacgcg tccagcgccg 3961 gcttggcgac gtcgtgaagg ctggcgatgt actcgccctg gtcgatagcc ccgaggccgc 4021 cggcatggca gcggagcgga aagtggccca ggccaaggcc gatctcgcgc gcaagaccta 4081 cgagcgcgag gcgagcctgt ttcagcaagg cgtgacaccg cgccaggaaa tggaggcggc 4141 aaaggctgct ctggatgttg cgcaagccga ggcgctgcgc gctgctacgg tggcccagtc 4201 cgctcacctt gcgtccgacg ggcgctcggt cgccgtggtc agcccgattg caggaaagat 4261 caccgcccag tccgtcaccc tgggtgcatt tgtggcgcca caagccgaac tctttcgggt 4321 agccggaact ggcgccgtac aggtagaagc cgccgtgacg gctgcagata ccagtcgcat 4381 cgtcgctggg agcgaagcca ccattctgtt ggccaatgga tcgccattgt cagcgcgcgt 4441 gcaggcggtg acgccaaccg tgacgggcag cgctcgcgtt gcaaccgtgg tggtggtacc 4501 agcacagcct acagatcgac ttgtcgttgg cgagggtgtg caggtgcgtc tgcgtacagc 4561 ggtggctgat gccgccgccc tgtccgtgcc ggaagacgcg gtgcagaacc tcgacggccg 4621 tgacgtccta ttcgtccgca cgcaggaagg cttccgccct atgcccgtcc tcgtcggcac 4681 ccgcagcggc ggctcggcac agattctgtc aggcgtgcag gctggagaac aggtggccac 4741 ccgtaatgcc ttcctggtta aggccgagat gaacaaaggc ggcggggacg aggaatgatc 4801 gagagcattc tcagcggctc agtccgctat cgctggctgg tcctgtttct gacagcagtt 4861 gtcgccgtaa ttggggcctg gcagctcaac ctgctcccca tcgacgtcac accggacatt 4921 accaacaagc aggtccagat caactccgtg gtgccaacga tgagccccgt ggaggtagaa 4981 aagcgtgtca cgtatccaat cgagacggcg attgctggtc tgaacggggt ggaaagcacg 5041 cgatcgatgt cgcgcaacgg cttcagccag gtgacggtga tcttcaagga gagtgccaac 5101 ctgtacttca tgcgccacga ggtgtcagaa cgactggcac aggcgcggcc gaatctgcca 5161 gaaaacgtcg agccacagat ggggcctgtt tcgacggggc tgggggaagt gttccactac 5221 agcgtggaat accagtatcc ggacggcaca ggggcctcca tcaaggacgg cgagcccgga 5281 tggcagagcg acggcagctt tctcaccgag cgtggcgaga ggttggacga tcgtgtgtca 5341 cgactggcct atttgcgtac ggtgcaagac tggatcatcc gcccgcaatt gcgcaccacg 5401 cctggtgtcg ccgatgtcga ttccctaggg ggctatgtca agcaatttgt ggtggagccg 5461 gacacgggga agatggcagc atacggagtt tcctacgcgg acctagcccg ggcgctcgaa 5521 gataccaacc tttctgtcgg cgcgaacttt attcggcgct caggcgagtc gtatctggtc 5581 cgcgccgacg cgcgtatcaa atctgcggac gagatatccc gtgccgtgat tgcgcacggc 5641 aaaatgtccc atcacgtcgg gcaggtagcg cgcgtcaaga ttggtggtga attgcgatca 5701 ggcgccgcca gccggaatgg caatgaaacg gtggtgggca gtgcgctgat gctggtcggt 5761 gccaacagcc ggacggtggc gcaagccgtg ggtgacaagc tcgaacagat ctcgaagacg 5821 ctgccaccag gcgtggtgat tgtcccgacg ctgaatcgtt cgcaactggt gattgcaacc 5881 atcgaaacgg tggcaaaaaa cctgatcgaa ggcgcgctgc tcgtggtggc gattctgttc 5941 gcgctgctgg gtaactggcg tgccgccacc attgccgcgc tagtcattcc gttgtccctg 6001 ctggtcagcg caatcggcat gaatcagttc cacatctcgg gcaacctgat gagcctgggt 6061 gcgctcgact tcggcctgat cattgacggc gcggtcatca tcgtcgagaa ctccctcagg 6121 cggctggccg agcgccagca ccgcgaaggg cgcctgctga cgctcgatga ccgtttgcag 6181 gaagttgtcc aatcgtcacg tgaaatggtg cgtccgacgg tctacggcca gcttgtgatc 6241 ttcatggtct tcctgccatc tctgacattc cagggcgtgg aaggcaagat gttctccccg 6301 atggtaatca cgctgatgct ggcgcttgcc tctgccttcg tgctgtcgct caccttcgtg 6361 ccggccatgg tcgccgtgat gctccggaaa aaggttgcag agacagaagt gcgcgtcatc 6421 gtggcgacca aagaaagtta ccggccatgg ctggagcatg ccgtggcacg cccaatgccc 6481 ttcatcggcg caggcatcgc gacagtcgct gtggctacag tggcgttcac atttgttggt 6541 cgcgagttta tgcccacgct ggatgagttg aacctgaacc tgtcgtcggt tcggattccg 6601 tctacgtcga ttgaccagtc ggttgccata gacctgccgc tcgaacgcgc cgtcttgtcg 6661 ctgccggaag tgcagactgt gtattcgaag gcgggtacgg ccagcctcgc cgccgacccc 6721 atgccgccca atgcatcgga taactacatc attctgaaac cgaaaagcga atggccggag 6781 ggcgtgacga ccaaggaaca ggtgattgag cgtatccgcg agaagacagc accgatggtc 6841 ggcaacaact acgacgtgac gcaaccgatc gagatgcgct ttaacgagct gatcggtggg 6901 gtccgaagcg acgtggccgt aaaggtgtac ggagagaatc tcgacgagtt ggcggccaca 6961 gcgcaacgca ttgccgcagt cttgaagaag acgccgggag cgacggacgt ccgtgtgcct 7021 ttgactagcg gctttccgac ctttgacatc gtgttcgacc gcgccgcaat cgcccgatat 7081 ggactcacgg tcaaggaagt cgccgatact atttccaccg ccatggcggg ccgacctgcc 7141 gggcagattt tcgatggtga ccgccgcttc gatattgtga tccgcttgcc cggtgagcag 7201 cgggaaaacc tcgacgtcct cggcgcgctt cccgtcatgt tgccgctatc ggaaggccag 7261 gcccgcgcct cggtgccttt gcgccaactg gtacagttcc ggttcacgca ggggctcaac 7321 gaagtaagcc gcgacaacgg aaagcggcgc gtctacgtgg aggccaatgt cggcggccgt 7381 gacctgggca gttttgtgga cgatgccgcg gcgcggatcg ccaaggaagt gaagttgccg 7441 ccgggtatgt acatcgaatg gggtggccaa tttcagaatc ttcaggcggc tacgaagcgc 7501 ctggccatca ttgtcccgct gtgctttatt ttgattgcgg cgacgctgta catggcgatc 7561 ggcagcgcgg cgctgacggc gaccgtgctg accgcctcgc cgctggcact tgcggggggc 7621 gtgtttgcgc tgctactgcg cgggattccg ttctctattt cggcggcagt tggcttcatt 7681 gcggtatccg gtgtggcggt gctgaacgga ctggtgctga tttccgctat ccgaaaacgc 7741 ctggacgatg ggatggcgcc tgacgctgcg gtgattgaag gtgcgatgga acgcgtgcgc 7801 ccggtactga tgacggctct ggtggcctct ctgggcttcg tgccgatggc gattgctaca 7861 ggtacaggtg ccgaagtcca gaaaccacta gctacggtcg tcatcggtgg actcgtcacc 7921 gcaactgtgc tgacactgtt tgtactgccg gccctttgcg gcatagtact gaagcgccgg 7981 accgctggcc gaccggaagc gcaagcagcg ctggaagcat gagtttgcga gtccacgctc 8041 ccactgcgcc ttgatccttg atttcccatg tctattcgta ccgcatctcc ggcattactg 8101 gccgctctgc ttttcggagc tagcacgccg ctggccaagg cgctaaccgg ttccgttgcc 8161 ccactcctgt tggcgggctt gctctatctt ggcagtggca tcggactggc gcttgtgctc 8221 gcaatccgaa gagcctggat cacgcctgac ccaggaaatg atgacgtggg tattccgcga 8281 ggggaagtgc cgtggctgat cggcgccatc ctcgctggcg gcgtagcggg gccagcgttg 8341 ctgatgaccg ggctcgtctc aaccaatgcc gcttcggcat ccctgctgct gaacgtggag 8401 ggtgtgttca cggccgtcat cgcctgggtg gtgttcaagg agaatgctga ccgccagatt 8461 gtcctgggga tgatcgcgat cgtggccggt ggccttctgc tgtcttggca gccgggcagc 8521 ctgcagatgt caaccggtgc gctgcttatc gtgggtgcct gcctgtgctg ggccgtagat 8581 aacaacctga cccgcaaggt ctccagcaat gacgcgctgc tcgtcgcctg cctgaagggg 8641 cttgtcgctg ggctatgcaa caccaccctc gccttgacgc atggggcggt gtttccatcg 8701 gtcagtactg cggcggcagc gctgatcatc ggattcgccg gatacggtgt aagcctcgcc 8761 ttgtttgtgg tggcgctgcg gctgttgggc actgcgcgaa ccggggcata tttctctgtc 8821 gccccattat ttggcgtcct gcttgcgttt gcgatctggc ctgaggtgcc aggcttcgca 8881 ttctgggcgg ctttggcttt gatggccttg ggtgtgtggt tacatctgcg agagaggcac 8941 gagcatgagc atacccatga gccactggag catagccact cgcatcggca cgacgaacac 9001 catcagcatg agcatgactt cccatgggat gggaaggaac cccacaggca ttggcatcgg 9061 cacgaggtga tggtgcacaa gcatccacac tacccggacg tgcatcatcg ccattctcac 9121 tagagttgct ttgcctcggt tctgcctggc aaaggcctct ataggtaaga ggctctcgac 9181 atcccgacga ctcagccgaa taggctcgtt gggtcatccg gtacgccatg tcgtcgctaa 9241 cgtctgcgcg gtcgtggcac tcggaagtag tcgatgcgcg tagcgcgcaa tcgggaaatc 9301 gaaagtaccg cgtaagttga tgccttccag ccgagtcggt gcgatctgcc gtaggtcctc 9361 ggcacgcatg gattcgccgc cgagagcttc tatcgcctcc agggcgtgct gcatgtgcag 9421 tgtgttccaa gccatcacgg cattggtcag cagcgtcaac gaggacgaga ccgctgccag 9481 ggacgcatcg tgtcgagcca actccaaggg aatttttccg taatggatgg cccgctgcac 9541 ggtatgcaca gcttcaccgc gattcagggc atgctgcatc tcttggcgga acgcggagtt 9601 ggtgaaatag tcgacgagga agatcgtacg gaatagcctt cccagatgca cgcctgcctc 9661 atagacgtgt tggccccgtg ccgccgcgcc gaaacgtgtc aatgcctgca ccgccgtgca 9721 ttggccggtc tggaccgacg ccgcgatccg gaccagttca tcccaggcaa tttcgatcaa 9781 caccagccga acatccgcgt ccgcgaccgc ggccagttct gacggaaccg catagccttt 9841 ggggacatgc aaccggcgat cacgcagatg ggagagccgc gggcagagat cgaagcccag 9901 caaccgtgac agactcatgg cgaagtcggt gtagccgtgg gtatcgacgg cgagttgcgt 9961 gacgtcttcc ccgccattct gacgaatcac gccctcgatg gcggcacccg cttgtctctc 10021 attcaagagg atcggctggt cgtagaagat gccccatcga tcgcggacat gggtgtacat 10081 cccaatcgac gcggtacgtc gacgcgggtc agtccgcgct tgccagaccg agcgcgtcgt 10141 ctcgag //