LOCUS       AI662208                 577 bp    mRNA    linear   EST 10-MAY-1999
DEFINITION  mz96f03.x1 Soares mouse lymph node NbMLN Mus musculus cDNA clone
            IMAGE:721277 3' similar to gb:U05252 Mus musculus nuclear matrix
            attachment DNA-binding protein SATB1 (MOUSE), mRNA sequence.
ACCESSION   AI662208
VERSION     AI662208.1
DBLINK      BioSample: SAMN00155172
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Marra,M., Hillier,L., Kucaba,T., Martin,J., Beck,C., Wylie,T.,
            Underwood,K., Steptoe,M., Theising,B., Allen,M., Bowers,Y.,
            Person,B., Swaller,T., Gibbons,M., Pape,D., Harvey,N., Schurk,R.,
            Ritter,E., Kohn,S., Shin,T., Jackson,Y., Cardenas,M., McCann,R.,
            Waterston,R. and Wilson,R.
  TITLE     The WashU-NCI Mouse EST Project 1999
  JOURNAL   Unpublished
COMMENT     Other_ESTs: mz96f03.y1
            Contact: Marra M/WashU-NCI Mouse EST Project 1999
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: mouseest@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            This clone was previously sequenced on the 5' end only, this new
            data is from the 3' end
            High quality sequence stop: 466.
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="taxon:10090"
                     /clone="IMAGE:721277"
                     /sex="male"
                     /tissue_type="lymph node"
                     /clone_lib="SAMN00155172 Soares mouse lymph node NbMLN"
                     /dev_stage="4 weeks"
                     /lab_host="DH10B"
                     /note="Organ: lymph node; Vector: pT7T3D-PacI; Site_1: Not
                     I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I
                     - oligo(dT) primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCGATACTTTTTTTTTTTTTTTTTTTTTTTT
                     3']; double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Not I and cloned into the Not I
                     and Eco RI sites of the modified pT7T3 vector. RNA
                     provided by Dr. Bertrand Jordan. Library constructed and
                     normalized by Bento Soares and M.Fatima Bonaldo."
BASE COUNT          164 a          130 c          117 g          163 t
ORIGIN      
        1 ctctttaaaa taaggggcca gtttccaaag actagtgtgg cgggttacag aagaattact
       61 aaagaaaagg caatcataac cacagaagtt ataatgctta tttgaggctc cagaataata
      121 ataatcaaaa ggaaaaccaa tttctggtat catgctgaca gggtacaaca cctgggtgca
      181 gcccttgaac acaaattcta ccaaacagtc cttgacgctt cacccggggc tgccaagcag
      241 ttttgtaaaa cacagtaaaa ccttgagtgc tggctgtcct gtccttccaa cttccttcct
      301 gtttgtgcaa gtttccgaag attcattggc caaacaataa acaatgaagg tttgtctgag
      361 tgaacacaag gtggacttct catgttgtta ggaaataccg gtggtgtggc tgaaaagaat
      421 actgttctct cagtctttca agtcggcatt aacgtctgtg ctcccttcca ccgacagctc
      481 ttnctctagt ttcactgaga aaaaggntgt tggcgtttta tcctggacgc tctctttcaa
      541 tccttaaagc acttctcatc tttgtactnc ggcacat
//