LOCUS       AI528521                 624 bp    mRNA    linear   EST 08-JAN-2011
DEFINITION  mq58e05.x1 Soares_thymus_2NbMT Mus musculus cDNA clone IMAGE:582944
            3' similar to gb:U05252 Mus musculus nuclear matrix attachment
            DNA-binding protein SATB1 (MOUSE), mRNA sequence.
ACCESSION   AI528521
VERSION     AI528521.1
DBLINK      BioSample: SAMN00155149
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 624)
  CONSRTM   NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap
  TITLE     National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
            Tumor Gene Index
  JOURNAL   Unpublished
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Possible reversed clone: polyT not found
            High quality sequence stop: 484.
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="taxon:10090"
                     /clone="IMAGE:582944"
                     /sex="male"
                     /tissue_type="Thymus"
                     /clone_lib="SAMN00155149 Soares_thymus_2NbMT"
                     /dev_stage="4 weeks"
                     /lab_host="DH10B"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     1st strand cDNA was primed with a Not I - oligo(dT) primer
                     [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCGTTTTTTTTTTTTTTTTTTTTTTTTT
                     3']; double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Not I and cloned into the Not I
                     and Eco RI sites of the modified pT7T3 vector. RNA
                     provided by Dr. Bertrand Jordan. Library went through two
                     rounds of normalization, and was constructed by Bento
                     Soares and M.Fatima Bonaldo."
BASE COUNT          148 a          164 c          194 g          117 t
ORIGIN      
        1 gcggccgcca gctcggcggc ggggaggtgg cagcagctgc agccgccgcc cgggaagtcc
       61 gccgcccggc gcgcccgcgc ggggagccga cccggcctcg gcccgcaccg ctggcccgcg
      121 ctgtcccggt tccacgcctg attctggcag gtgatctgta agacagtgac tgagtatgga
      181 tcatttgaac gaggcaactc aggggaaaga acattcagaa atgtctaaca atgtgagtga
      241 tccgaagggt ccacccgcca agattgcccg cctggagcag aacgggagcc ctctaggaag
      301 aggaaggctt gggagcacag gtggaaagat gcagggagtg cctttaaaac actcgggcca
      361 tctcatgaaa accaacctta ggaaaggaac catgttacca gttttctgcg tggtggaaca
      421 ttatgaaaac gccattgagt atgattgcaa ggaggagcac gcggaatttg tattggtgag
      481 aaaggatatg cttttcaacc agctgataga gatggcgttg ctgtctctag gctattcaca
      541 cagctctgct gccccagcaa aggctcatcc agntgggaag tggatccagt tcactgtcga
      601 tgtgacagat gccctgatgc cacg
//