LOCUS AI528521 624 bp mRNA linear EST 08-JAN-2011 DEFINITION mq58e05.x1 Soares_thymus_2NbMT Mus musculus cDNA clone IMAGE:582944 3' similar to gb:U05252 Mus musculus nuclear matrix attachment DNA-binding protein SATB1 (MOUSE), mRNA sequence. ACCESSION AI528521 VERSION AI528521.1 DBLINK BioSample: SAMN00155149 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 624) CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Possible reversed clone: polyT not found High quality sequence stop: 484. FEATURES Location/Qualifiers source 1..624 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="taxon:10090" /clone="IMAGE:582944" /sex="male" /tissue_type="Thymus" /clone_lib="SAMN00155149 Soares_thymus_2NbMT" /dev_stage="4 weeks" /lab_host="DH10B" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. RNA provided by Dr. Bertrand Jordan. Library went through two rounds of normalization, and was constructed by Bento Soares and M.Fatima Bonaldo." BASE COUNT 148 a 164 c 194 g 117 t ORIGIN 1 gcggccgcca gctcggcggc ggggaggtgg cagcagctgc agccgccgcc cgggaagtcc 61 gccgcccggc gcgcccgcgc ggggagccga cccggcctcg gcccgcaccg ctggcccgcg 121 ctgtcccggt tccacgcctg attctggcag gtgatctgta agacagtgac tgagtatgga 181 tcatttgaac gaggcaactc aggggaaaga acattcagaa atgtctaaca atgtgagtga 241 tccgaagggt ccacccgcca agattgcccg cctggagcag aacgggagcc ctctaggaag 301 aggaaggctt gggagcacag gtggaaagat gcagggagtg cctttaaaac actcgggcca 361 tctcatgaaa accaacctta ggaaaggaac catgttacca gttttctgcg tggtggaaca 421 ttatgaaaac gccattgagt atgattgcaa ggaggagcac gcggaatttg tattggtgag 481 aaaggatatg cttttcaacc agctgataga gatggcgttg ctgtctctag gctattcaca 541 cagctctgct gccccagcaa aggctcatcc agntgggaag tggatccagt tcactgtcga 601 tgtgacagat gccctgatgc cacg //