LOCUS AI060807 501 bp mRNA linear EST 22-JUL-1998 DEFINITION ub44b05.r1 Soares_thymus_2NbMT Mus musculus cDNA clone IMAGE:1380561 5' similar to gb:U05252 Mus musculus nuclear matrix attachment DNA-binding protein SATB1 (MOUSE), mRNA sequence. ACCESSION AI060807 VERSION AI060807.1 DBLINK BioSample: SAMN00155149 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 501) AUTHORS Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T., Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M., Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B., Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and Waterston,R. TITLE The WashU-HHMI Mouse EST Project JOURNAL Unpublished COMMENT Contact: Marra M/Mouse EST Project WashU-HHMI Mouse EST Project Washington University School of MedicineP 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: mouseest@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. MGI:903029 Seq primer: -28m13 rev2 ET from Amersham High quality sequence stop: 494. FEATURES Location/Qualifiers source 1..501 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="taxon:10090" /clone="IMAGE:1380561" /sex="male" /tissue_type="Thymus" /clone_lib="SAMN00155149 Soares_thymus_2NbMT" /dev_stage="4 weeks" /lab_host="DH10B" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. RNA provided by Dr. Bertrand Jordan. Library went through two rounds of normalization, and was constructed by Bento Soares and M.Fatima Bonaldo." BASE COUNT 114 a 137 c 109 g 141 t ORIGIN 1 cacagggcat ctgtcacata agacagtgga actggattcc acttcactac ctggatgagc 61 cctttggctt gggcagcaga gtgtgttgaa tagcctagag acagcaacgt atctctatca 121 gctggttgaa aagcatatcc tttctcacca atacaaattc cgcgtgctcc tccttgcaat 181 catactcaat ggcgttttca taatgttcca ccacgcagaa aactggtaac atggttcctt 241 tcctaaggtt ggttttcatg agatggcccg agtgttttaa aggcactccc tgcatctttc 301 cacctgtgct cccaagcctt cctcttccta gagggctccc gttctgctcc aggcgggcaa 361 tcttggcggg tggaccttcg gatcactcac attgttagac atttctgaat gttctttcgc 421 ctgagttgcc tcgttcaaat gatccatact cagtcactgt cttacagatc acctgccaga 481 atcaggcgtg gaaccgggac a //