LOCUS AF486630 6320 bp DNA linear BCT 02-DEC-2002 DEFINITION Amycolatopsis orientalis peptide synthetase gene, partial cds; hypothetical protein, OxyA (oxyA), OxyB (oxyB), and OxyC (oxyC) genes, complete cds; and halogenase gene, partial cds. ACCESSION AF486630 VERSION AF486630.1 KEYWORDS . SOURCE Amycolatopsis orientalis ORGANISM Amycolatopsis orientalis Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales; Pseudonocardiaceae; Amycolatopsis. REFERENCE 1 (bases 1 to 6320) AUTHORS Zerbe,K., Pylypenko,O., Vitali,F., Zhang,W., Rouset,S., Heck,M., Vrijbloed,J.W., Bischoff,D., Bister,B., Sussmuth,R.D., Pelzer,S., Wohlleben,W., Robinson,J.A. and Schlichting,I. TITLE Crystal structure of OxyB, a cytochrome P450 implicated in an oxidative phenol coupling reaction during vancomycin biosynthesis JOURNAL J. Biol. Chem. 277 (49), 47476-47485 (2002) PUBMED 12207020 REFERENCE 2 (bases 1 to 6320) AUTHORS Zhang,W., Zerbe,K., Vrijbloed,J.W. and Robinson,J.A. TITLE Direct Submission JOURNAL Submitted (22-FEB-2002) Institute of Organic Chemistry, University of Zuerich, Winterthurerstrasse 190, Zuerich 8057, Switzerland FEATURES Location/Qualifiers source 1..6320 /organism="Amycolatopsis orientalis" /mol_type="genomic DNA" /db_xref="taxon:31958" CDS <1..723 /function="involved in the biosynthesis of vancomycin heptapeptide backbone" /codon_start=1 /transl_table=11 /product="peptide synthetase" /protein_id="AAL90875.1" /translation="GDRPPLFCVHASGGLSWNYEPLLRYLPANQPVYGVQARGLARTE PLPGSVEEMAADYLAQIRAVQPSGPYHLLGWSLGGRIAQAMARLLEQEGEKVGLLALL DAYPVYMGRKTTGAASEEAALSKRNQADLDLAGQLVKSEAARARLEAVMRNLWDIGPR HVSSPFAGDILLFVATVDRPEHLPVSVAKASWKEFTTGTIEAHDIPSNHYDMVQTAAL GQIGSIVAEKLRQRPEGERTQR" CDS 720..929 /inference="non-experimental evidence, no additional details recorded" /note="orf1" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AAL90876.1" /translation="MTNPFDNEDGSFFVLVNDEGQHSLWPSFAEVPSGWTRVLGETTR QECLAYVEENWTDLRPKSLIQEAGA" gene 1066..2241 /gene="oxyA" CDS 1066..2241 /gene="oxyA" /function="involved in the coupling of aromatic side chains of the heptapeptide" /note="P450 monooxygenase" /codon_start=1 /transl_table=11 /product="OxyA" /protein_id="AAL90877.1" /translation="MFEEKNALRGTEIHRRERFDPGPELRALMAEGRMSVMESEESPG GRTGWLATGYEETRQVLGSDKFSAKLLFGGTAAGRIWPGFLNQYDPPEHTRLRRMVAS AFTVRRMRDFRPRIEAVVKATLDDIEATGGPVDFVPRFAWPIATTVICDFLGIPRDDQ AELSRVLHASRSERSGKRRVAAGNKYWTYMGQVAAKTRRDPGDDMFGAVVREHGDDIT DAELLGVAAFVMGASGDQVARFLSAGAWLMVEHPEQFAVLRDDPDSVPDWLNEVARYL TSDEKTTPRIALEDVRIGDQLVKKGDAVTCSLLASNRHRFPDPEDRFDITREKPSHVT FGHGIHHCLGRPLAEMVFRTAIPALAHRFPTLRLAEPDREIKLGPPPFDVEALLLDW" gene 2645..3841 /gene="oxyB" CDS 2645..3841 /gene="oxyB" /function="involved in the coupling of aromatic side chains of the heptapeptide" /note="P450 monooxygenase" /codon_start=1 /transl_table=11 /product="OxyB" /protein_id="AAL90878.1" /translation="MSEDDPRPLHIRRQGLDPADELLAAGALTRVTIGSGADAETHWM ATAHAVVRQVMGDHQQFSTRRRWDPRDEIGGKGIFRPRELVGNLMDYDPPEHTRLRRK LTPGFTLRKMQRMAPYIEQIVNDRLDEMERAGSPADLIAFVADKVPGAVLCELVGVPR DDRDMFMKLCHGHLDASLSQKRRAALGDKFSRYLLAMIARERKEPGEGMIGAVVAEYG DDATDEELRGFCVQVMLAGDDNISGMIGLGVLAMLRHPEQIDAFRGDEQSAQRAVDEL IRYLTVPYSPTPRIAREDLTLAGQEIKKGDSVICSLPAANRDPALAPDVDRLDVTREP IPHVAFGHGVHHCLGAALARLELRTVFTELWRRFPALRLADPAQDTEFRLTTPAYGLT ELMVAW" gene 3970..5190 /gene="oxyC" CDS 3970..5190 /gene="oxyC" /function="involved in the coupling of aromatic side chains of the heptapeptide" /note="P450 monooxygenase" /codon_start=1 /transl_table=11 /product="OxyC" /protein_id="AAL90879.1" /translation="MGHDIDQVAPLLREPANFQLRTNCDPHEDNFGLRAHGPLVRIVG ESSTQLGRDFVWQAHGYEVVRRILGDHEHFTTRPQFTQSKSGAHVEAQFVGQISTYDP PEHTRLRKMLTPEFTVRRIRRMEPAIQSLIDDRLDLLEAEGPSADLQGLFADPVGAHA LCELLGIPRDDQREFVRRIRRNADLSRGLKARAADSAAFNRYLDNLLARQRADPDDGL LGMIVRDHGDNVTDEELKGLCTALILGGVETVAGMIGFGVLALLDNPGQIELLFESPE KAERVVNELVRYLSPVQAPNPRLAIKDVVIDGQLIKAGDYVLCSILMANRDEALTPDP DVLDANRAAVSDVGFGHGIHYCVGAALARSMLRMAYQTLWRRFPGLRLAVPIEEVKYR SAFVDCPDQVPVTW" CDS 5235..>6320 /codon_start=1 /transl_table=11 /product="halogenase" /protein_id="AAL90880.1" /translation="MSVEDFDVVVAGGGPGGSTVATLVAMQGHRVLLLEKEVFPRYQI GESLLPATVHGVCRMLGVADELAGAGFPIKRGGTFRWGPRPEPWTFHFGISAKMAGST SHAYQVERARFDEILLNNAKSKGVVVREGCSVHDVVEDGERVTGAKYTDPDGNEREVS ARFVIDASGNKSRLYSKVGGSRNYSEFFRSLALFGYFEGGKRLPEPVSGNILSVAFDN GWFWYIPLSDTLTSVGAVVRREDADKIQGDREKALNALIAECPLISEYLSNATRVTTG KYGELRVRKDYSYQQETYWRPGMVLVGDAACFVDPVFSSGVHLATYSALLAARSINSV LAGDLDEKTALNEFEARYRREYGVFYEF" BASE COUNT 1090 a 2048 c 2163 g 1019 t ORIGIN 1 ggcgaccgcc cgccgctgtt ctgtgtccac gcgagcggtg gcctgagctg gaactacgaa 61 ccgttgctgc ggtatctccc ggcgaaccag ccggtctacg gcgtgcaggc acgcggcctg 121 gccaggacgg aacccctgcc gggcagcgtc gaggagatgg cggccgacta cctcgcgcag 181 atccgcgccg tgcagccgtc cgggccgtac cacctcctcg gctggtccct cggcggccgg 241 atcgcgcagg cgatggccag gctgctggag caggaagggg agaaggtcgg cctgctggcg 301 ctgctcgacg cctatcccgt ctacatggga cgcaagacga ccggcgccgc gagcgaagaa 361 gcggccctgt cgaagcggaa ccaggcggat ctggacctcg cgggtcaact ggtgaagagc 421 gaggccgccc gggcgcgcct cgaagccgtc atgcggaacc tctgggacat cgggccacgg 481 cacgtttcct cgcccttcgc cggcgacatc ctgctcttcg tggccactgt ggaccgtccc 541 gaacatcttc ccgtctcggt ggcgaaagcc agctggaagg agttcaccac cgggaccatc 601 gaggcccacg acatcccgtc caaccactac gacatggtgc aaaccgcggc gctgggccag 661 attggatcca tcgtcgccga gaaactccgg cagcggccgg aaggtgaaag gacacaacga 721 tgaccaaccc cttcgacaac gaagacggtt cctttttcgt tctggtcaac gacgaggggc 781 agcactccct gtggccgtcc ttcgccgagg tgccgtcggg ctggacccgg gtgctcggcg 841 agaccacccg ccaggaatgc ctcgcctatg tcgaggagaa ctggacggac ctccggccga 901 agagcctcat ccaggaagcg ggcgcctgag cgaacgggtg tccagccgct caacgcggac 961 ggttggacgg tgcggtggcg tagttgggcc gtgaaggctc cttgagggaa ccagagtccc 1021 tcaaggagcc attcacggac cacccctgcg aaaggacctt gtgcagtgtt cgaggagaag 1081 aacgccctcc ggggaacgga aatccaccgg agagagcggt tcgacccggg accggagctg 1141 cgtgcgctga tggccgaagg ccgcatgtcc gtgatggagt cggaagagtc cccgggcggg 1201 cgtaccgggt ggctcgccac cgggtacgag gagacccgcc aggtgctcgg ctcggacaag 1261 ttcagcgcca agctgctgtt cggcgggacg gcggcgggcc gcatctggcc cgggttcctc 1321 aaccagtacg acccgccgga gcacacgcgc ctgcgccgga tggtggcctc cgcgttcacc 1381 gtccggcgga tgcgggattt ccggccgcgg atcgaggcgg tcgtcaaggc caccctggac 1441 gacatcgagg ccaccggcgg gccggtggat ttcgtgcccc ggttcgcgtg gcccatcgcg 1501 acgacggtga tctgcgactt cctcggcatt ccccgtgacg atcaggcgga gctgtcgcgc 1561 gtcctgcacg ccagccggtc cgaacggtcg ggcaaacggc gggtggcggc ggggaacaag 1621 tactggacgt acatgggcca ggtcgcggcc aaaacgcgtc gcgatcccgg tgacgacatg 1681 ttcggtgccg tggtgcgcga acacggcgac gacatcaccg acgcggaact gctgggcgtc 1741 gccgcgttcg tgatgggcgc cagcggggat caggtggcgc ggttcctctc ggcgggtgcg 1801 tggctgatgg tcgagcaccc cgagcagttc gccgtgctgc gcgacgaccc ggacagcgtc 1861 ccggattggc tgaacgaggt ggcccgctac ctcaccagcg acgagaagac cactccgcgc 1921 atcgcgctgg aggacgtgcg gatcggtgac cagctcgtca agaagggcga tgccgtcacc 1981 tgttccctgc tggcttcgaa ccggcacagg ttccccgacc cggaggaccg gttcgacatc 2041 acccgggaaa agccctcgca tgtcacgttc gggcacggta tccatcactg cctcggccgt 2101 ccgctggcgg aaatggtgtt ccggacggcg attccggcgc tggcgcaccg gtttcccacg 2161 ctcaggctgg ccgaaccgga tcgcgagatc aaactgggcc cgccgccgtt cgacgtggaa 2221 gccctgctgc tcgactggtg acgccggtgg tcggtggcgg ccgtgtaggc gtcaccacgc 2281 cgtgaaggcc tccttaccta ccttgagggt agggaagggg cccttcacta cctggcagca 2341 ggcaagggac tgtccgctga ggcggggcgc gtcccatgac ggtcctgaac ggggtcgtga 2401 gtggcaaaga gcgttctaac gctctttgcc actcacgacc acctcgcgaa accatgcaaa 2461 ctgctcaaat cggacgctta tctcccgaaa agtgtctctt gtggacaccc ggagccgcga 2521 cccggcttcc gtcagccggt ccggacccgc gccgggaggt cggtaccggt ggtcgtgagt 2581 ggtaatgacg gttctaaccc tcattaccac tcacgactcc ttcccaagca aaggagtttt 2641 cccgttgagc gaggacgacc cgcgcccgct gcacattcgc aggcagggct tggatccggc 2701 ggacgaactg ctcgccgccg gagcgctgac gagggtcacc atcggatccg gagcggacgc 2761 cgagacccat tggatggcca ccgcgcacgc cgtcgtccgg caggtgatgg gtgatcacca 2821 gcagttcagc acccggcgcc gctgggaccc gcgggacgag atcggcggga agggcatctt 2881 ccggccgcgg gaactggtcg gcaacctgat ggactacgac ccgcccgagc acacgcggct 2941 gcggcggaaa ctgacgcccg gcttcaccct ccggaaaatg cagcggatgg cgccgtacat 3001 cgagcagatc gtgaacgacc gcctcgacga gatggagcgg gcgggatccc ccgcggatct 3061 gatcgcgttc gtcgccgaca aggtgcccgg cgccgtgctg tgcgagctgg tcggggtgcc 3121 gagggacgac cgcgacatgt tcatgaagct ctgtcacggg catctcgacg cctcgctgag 3181 tcaaaagcgc cgggcggcgc tcggcgacaa gttctcgcgc tatctgctgg cgatgatcgc 3241 cagggaacgc aaggagccgg gtgaggggat gatcggcgcg gtcgtcgccg aatacgggga 3301 cgacgccacg gacgaggagc tacgcggttt ctgcgttcag gtgatgctgg cgggcgacga 3361 caacatctcc ggcatgatcg gcctcggtgt gctggcgatg ctgcgccatc ccgagcagat 3421 cgacgctttc cgcggcgacg agcagtcggc gcaacgggcg gtcgacgagc tgatccggta 3481 cctgaccgtg ccgtacagcc cgactccccg gatcgccagg gaggacctca ccctcgcggg 3541 gcaggagatc aagaaggggg acagcgtcat ctgctcgctc cccgcggcca accgtgatcc 3601 cgcgctggca ccggatgtgg accgcctcga cgtcacccgc gagcccatcc cgcatgtcgc 3661 cttcgggcac ggagtccacc attgcctcgg cgccgcgctg gcccggctcg aactgcgcac 3721 ggtcttcacc gagctgtggc ggcgtttccc ggcgctgagg ctcgcggatc ccgcacagga 3781 caccgaattc cggctgacca ctcccgcgta cggattgacc gagctgatgg tcgcctggtg 3841 atcgacggct ccgtccacgc gaggtcgctg gacgtgccgc ctgattaccg gtgaaattgg 3901 gacgtcggcg tgttcgggtc gataaagccg aacggcactt tcccggtcaa ccaaggagct 3961 tccagtcgga tggggcacga tatcgatcag gtcgcgccgc tcctgcggga gccggcgaac 4021 tttcagctga ggacgaactg cgatccgcac gaggacaatt tcgggctcag ggcgcacggc 4081 cctttggtgc ggatagtcgg cgaatcgtcc acccagctgg gcagggactt cgtctggcag 4141 gcccacggtt acgaggtcgt gcgcaggata ttgggtgatc acgaacattt cacgacgcgg 4201 ccgcaattca cccagtcgaa atccggcgcc catgtggagg cccagttcgt cgggcagatc 4261 tccacttatg acccacccga gcacacccgt ttgcggaaaa tgctcacgcc ggagttcacg 4321 gtccgccgga tccgccggat ggagcccgcg atccaaagtc tcatcgatga tcgcctcgat 4381 ctgctggagg ccgaggggcc gtccgcggac ctccagggcc tgttcgccga tccggtcggc 4441 gcgcacgccc tgtgcgaact gctcggcatc ccgcgcgacg accagcgtga gttcgtccgg 4501 cggatcaggc ggaacgccga cctgagccgg ggactcaagg cacgggccgc cgacagcgcg 4561 gcgttcaacc ggtatttgga caacctcctc gccaggcagc gcgccgaccc cgacgacggt 4621 ctcctcggca tgatcgtgcg ggaccacggc gacaacgtca cggacgagga actgaagggc 4681 ctctgcacgg cgctgatcct cggcggtgtc gaaaccgtcg ccgggatgat cggtttcggt 4741 gtcctcgccc tcctggacaa tcccggacag atcgagttgc ttttcgagtc ccccgaaaag 4801 gccgaacgcg tcgtcaacga actcgtccgg tatctgtccc cggtgcaggc gccgaatccg 4861 cgtctcgcca tcaaggacgt ggtcatcgac gggcagctga tcaaggcggg ggactacgtc 4921 ctgtgctcga ttctcatggc caaccgggat gaggcgctga ccccggatcc cgacgtcctc 4981 gacgcgaatc gcgccgcggt gtcggacgtc ggattcgggc acggcatcca ttactgcgtc 5041 ggcgcggcct tggccagatc catgctgcgc atggcttatc agactttgtg gcggcgattc 5101 ccgggactgc ggctggcagt gcccatcgaa gaagtgaagt accgcagcgc gttcgtagat 5161 tgccctgatc aggtaccggt cacctggtga ccgcaggccc ggcgaaatga cacacgacag 5221 aggaatggtg ggaaatgtcg gtcgaagatt tcgatgtggt ggttgccggt ggcgggccgg 5281 gtggttcgac cgtggccaca ctggtcgcga tgcagggcca tcgggtgctg ttgctggaga 5341 aggaggtctt tccccggtac cagatcggtg aatcgctgct gcccgccacg gtgcacggcg 5401 tgtgccggat gctcggcgtc gccgacgagc tggcgggggc ggggttcccg atcaagcggg 5461 gcggcacgtt ccgctggggt ccccggccgg agccgtggac attccacttc ggcatctccg 5521 ccaagatggc cggctcgacg tcgcacgcct accaggtcga gcgggcgcgg ttcgacgaga 5581 tcctgctgaa caacgccaaa agcaagggcg tcgtcgtgcg ggaaggctgc tctgtccacg 5641 atgtggtgga agacggtgag cgggtcaccg gcgcgaagta caccgacccc gacggcaacg 5701 agcgtgaggt gtcggcgcgt ttcgtgatcg acgcgtcggg caacaagagc cgcctctatt 5761 ccaaggtcgg cggttcacgg aactactcgg agttcttccg cagcctcgcg ttgttcggct 5821 acttcgaggg cggcaagcgg ctgccggagc cggtgtcggg gaacatcctg agcgtggcct 5881 tcgacaacgg ctggttctgg tacatcccgt tgagcgacac gctcaccagt gtcggcgcgg 5941 tggtgcgccg ggaggacgcc gacaagatcc agggtgaccg cgagaaggcc ctcaacgcct 6001 tgatcgccga atgcccgctg atctcggaat acctctcgaa cgcgaccagg gtgacgaccg 6061 gcaagtacgg ggaactgcgt gtccgcaagg actattcgta ccagcaggag acctactggc 6121 ggccgggcat ggtcctggtc ggcgacgccg cgtgcttcgt ggacccggtg ttctcctccg 6181 gggtgcacct ggcgacctac agcgcgctgc tcgcggcccg gtcgatcaac agcgtcctcg 6241 ccggcgatct cgacgagaag accgcgctga acgagttcga agcgcggtat cgccgggaat 6301 acggcgtctt ctacgaattc //