LOCUS AF102623 7186 bp DNA linear BCT 06-OCT-2006 DEFINITION Methanosarcina barkeri dimethylamine corrinoid protein MtbC (mtbC), trimethylamine methyltransferase MttB (mttB), trimethylamine corrinoid protein MttC (mttC), putative transmembrane protein MttP (mttP), and dimethylamine methyltransferase MtbB1 (mtbB1) genes, complete cds. ACCESSION AF102623 VERSION AF102623.1 KEYWORDS . SOURCE Methanosarcina barkeri ORGANISM Methanosarcina barkeri Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia; Methanosarcinales; Methanosarcinaceae; Methanosarcina. REFERENCE 1 (bases 1 to 7186) AUTHORS Ferguson,D.J. Jr. and Krzycki,J.A. TITLE Reconstitution of trimethylamine-dependent coenzyme M methylation with the trimethylamine corrinoid protein and the isozymes of methyltransferase II from Methanosarcina barkeri JOURNAL J. Bacteriol. 179 (3), 846-852 (1997) PUBMED 9006042 REFERENCE 2 (bases 1 to 7186) AUTHORS Paul,L., Ferguson,D.J. Jr. and Krzycki,J.A. TITLE The trimethylamine methyltransferase gene and multiple dimethylamine methyltransferase genes of Methanosarcina barkeri contain in-frame and read-through amber codons JOURNAL J. Bacteriol. 182 (9), 2520-2529 (2000) PUBMED 10762254 REFERENCE 3 (bases 1 to 7186) AUTHORS Ferguson,D.J. Jr., Gorlatova,N., Grahame,D.A. and Krzycki,J.A. TITLE Reconstitution of dimethylamine:coenzyme M methyl transfer with a discrete corrinoid protein and two methyltransferases purified from Methanosarcina barkeri JOURNAL J. Biol. Chem. 275 (37), 29053-29060 (2000) PUBMED 10852929 REFERENCE 4 (bases 1 to 7186) AUTHORS Soares,J.A., Zhang,L., Pitsch,R.L., Kleinholz,N.M., Jones,R.B., Wolff,J.J., Amster,J., Green-Church,K.B. and Krzycki,J.A. TITLE The residue mass of L-pyrrolysine in three distinct methylamine methyltransferases JOURNAL J. Biol. Chem. 280 (44), 36962-36969 (2005) PUBMED 16096277 REFERENCE 5 (bases 1 to 7186) AUTHORS Paul,L. and Krzycki,J.A. TITLE Direct Submission JOURNAL Submitted (27-OCT-1998) Microbiology, Ohio State University, 484 West 12th Ave, Columbus, OH 43210, USA FEATURES Location/Qualifiers source 1..7186 /organism="Methanosarcina barkeri" /mol_type="genomic DNA" /strain="MS" /type_material="type strain of Methanosarcina barkeri" /db_xref="taxon:2208" gene 734..1375 /gene="mtbC" CDS 734..1375 /gene="mtbC" /function="participates in dimethylamine:coenzyme M methyl transfer" /note="ORF identified by N-terminal sequence of isolated corrinoid protein" /codon_start=1 /transl_table=11 /product="dimethylamine corrinoid protein MtbC" /protein_id="AAD14629.1" /translation="MSKEELLQELADAIISCKKDTVLAVVEKAKGELEPSEIIEKGLA AGMNEVGVRFERGKLFLPHVMMAADAMTAGVAALKDLMPEGASGSKLGVIVNGTVEGD VHDIGKAIVSTMLQSAGFEVHDIGRDVPIRNFIEKAKEVNADMIGISALMTTTLQGQK SVIELLKEEGLRDKVKVMVGGAPATQAWADKIGADCYAENATEAVAKAKELLA" gene 1402..2889 /gene="mttB" CDS 1402..2889 /gene="mttB" /note="ORF identified with N-terminal sequence of 52 kDa trimethylamine methyltransferase polypeptide" /codon_start=1 /transl_except=(pos:2401..2403,aa:Pyl) /transl_table=11 /product="trimethylamine methyltransferase MttB" /protein_id="AAD14630.2" /translation="MAKNNAVAGFNALNGVELNLFTTDELKAIHYATMEVLMDPGIQV SDPEARQIFKENGCEVNEKTNVVKIPEYLVRKALQLAPSRFVLWGRDKKFNTVQECGG KVHWTCFGTGVKVCKYQDGKYVTVDSVEKDIADIAKLCDWAENIDYFSLPVSARDIAG QGAQDVHETLTPLANTAKHFHHIDPVGENVEYYRDIVKAYYGGDEEEARKKPIFSMLL CPTSPLELSVNACQVIIKGARFGIPVNVLSMAMSGGSSPVYLAGTLVTHNAEVLSGIV LAQLTVPGAKVWYGSSTTTFDLKKGTAPVGSPELGLISAAVAKLAQFYGLPSYVAGSO SDAKVPDDQAGHEKTMTTLLPALAGANTIYGAGMLELGMTFSMEQLVIDNDIFSMVKK AMQGIPVSEETLAVESIQKVGIGNNFLALKQTRQLVDYPSNPMLLDRHMFGDWAAAGS KDLATVAHEKVEDVLKNHQVTPIDADIFKDMQAIVDKADKAFRGM" gene 2920..3573 /gene="mttC" CDS 2920..3573 /gene="mttC" /function="participates in trimethylamine:coenzyme M methyl transfer with TMA methyltransferase" /note="ORF identified by N-terminal sequence of corrinoid protein" /codon_start=1 /transl_table=11 /product="trimethylamine corrinoid protein MttC" /protein_id="AAD14631.1" /translation="MANKEEIIAKAKEAITDFDDELAEEVANEALAAGIDPVELIEKG FTAGMEEVGEKFGQGELFLPHVLAAAEAMNSGIKVITPEMEKRKSQTKSLGTVAIGTI EGDIHSIGKDIVASMLNIAGFKVVDLGRDVPINTFVEKVKELKPQVVASSALMTTTMV NQIQIEEQLKEAGVRDQVKTMVGGAPVTQDWADKIGADIYGESANDAVAKVKAALNV" gene 3885..4946 /gene="mttP" CDS 3885..4946 /gene="mttP" /function="possible methylamine permease" /codon_start=1 /transl_table=11 /product="putative transmembrane protein MttP" /protein_id="AAD14632.1" /translation="MEGVFVDLKALKKQESKRKISKGYMWAFFCAIFWGIWYLPGTVV WVLNPFDEMSSAIAKTSGDGISLVVTAVLITAFNALAVMLALMVWNGVLGKYGELVRT LKEFHPCSKWFFLASIFGGPMAILGSFIAMGFIGGSFAAVAGLLYPVVGSILAYYWYG EKISKRAAIGIAVIVLGGISIYGGGLFTELSSGNVPWIGYLGGLMAAAGWGIEGAIAG KGLDIAEPDAGLTLRFLGENIIWWIIIVPVLALVGFPMYSFALQAFDPLTILILVFAG ITFGFCYVTWYKSFPLIGVGRGQGIGNLYGLCAIIFLFLFFGDVPDWTIIIGGALCIA GSFIMFTEDASELETLRGE" gene 5470..6873 /gene="mtbB1" CDS 5470..6873 /gene="mtbB1" /note="ORF identified by N-terminal sequence of 50 kDa DMA methyltransferase polypeptide" /codon_start=1 /transl_except=(pos:6535..6537,aa:Pyl) /transl_table=11 /product="dimethylamine methyltransferase MtbB1" /protein_id="AAD14633.2" /translation="MATEYALRMGDGKRVYLTKEKIVSEIEAGTADAADLGEIPALSA NEMDKLAEILMMPGKTVSVEQGMEIPVTHDIGTIRLDGDQGNSGVGIPSSRLVGCMTH ERAFGADTMELGHIDYSFKPVKPVVSNECQAMEVCQQNMVIPLFYGAMPNMGLYYTPD GPFENPGDLMKAFKIQEAWESMEHAAEHLTRDTVWVMQKLFASGADGVNFDTTGAAGD GDMYGTLHAIEALRKEFPDMYIEAGMAGECVLGMHGNLQYDGVTLAGLWPHQQAPLVA KAGANVFGPVCNTNTSKTSAWNLARAVTFMKAAVEASPIPCHVDMGMGVGGIPMLETP PIDAVTRASKAMVEIAGVDGIOIGVGDPLGMPIAHIMASGMTGMRAAGDLVARMEFSK NMRIGEAKEYVAKKLGVDKMDLVDEHVMRELREELDIGIITSVPGAAKGIAAKMNIEK LLDIKINSCNLFRKQIA" BASE COUNT 2127 a 1507 c 1692 g 1860 t ORIGIN 1 atgttgaatc atcaacaatt aggtatcctc ccttaagctt gacataactt tttacttcct 61 cccatagtgc ttccgtgtct ggaggttgcc tttgaaggca acgagtaaaa gcatcatgag 121 aaggagcatt agctatgtct ggataacatc tagcagcttc agtacagcta aaaacgttag 181 aagccgcaat gagaaaatta atgtagtcaa tgtcggtaca cttaggtgga tttatgtcca 241 taactccgta cgtttttaga gaaacactaa gccataagta tatttataaa gatatcaagt 301 tttcggcatt ttaactgcgt aagtcctata tacaaataaa accggaaaaa ccaaaaacta 361 taaatattac ccccaaattc attacagaat ttttagaaaa atatatatat tcgaatgacc 421 aacaaagtat tagagacatc tgagaaatga gttatcagac gggattaaaa tggcacgaaa 481 aacactccct ctgctaaaac actcatgaac cccaatcagc gtaatcatga attcctcatg 541 attttattgt ctcgcttgag gtgatcatgg cttcccaccc atggtgatcg tgagtttctt 601 cctgtatatt catcgcttca cccagaagca tgagcttaca gcctgaatgt tcacgaataa 661 tcgtgacaga ataatcgtga taattacagg agagaagtct ctatagttca aaaactaaaa 721 cggaggatgc aacattagca aagaagaatt gcttcaggaa cttgcagatg ctataatttc 781 ctgcaagaag gatacagtac tcgctgtagt tgaaaaagct aaaggagaac tggaaccttc 841 cgaaataata gaaaaaggac ttgcagcagg catgaatgag gttggtgtcc gtttcgagag 901 aggaaaattg tttctgccac atgtgatgat ggctgccgat gcgatgactg caggagttgc 961 agctcttaag gatcttatgc ctgaaggggc ttccggctca aagctcggtg ttattgtgaa 1021 cggtaccgta gaaggtgatg ttcatgatat tggaaaggca atcgtatcca caatgcttca 1081 atctgccggc tttgaagtac atgacattgg ccgtgatgtt ccaatcagga attttattga 1141 gaaggcaaag gaagtcaatg ctgatatgat tggaatttcc gctcttatga ccacaactct 1201 ccagggacag aaaagtgtaa ttgagctcct caaagaagaa ggactcagag ataaagtaaa 1261 agtcatggta ggcggtgcac ctgcaaccca ggcctgggct gacaaaatcg gtgcagactg 1321 ctatgctgag aatgcaaccg aagctgttgc aaaagcaaaa gagctgctgg cttaaatcat 1381 tcttattcgg aggttataaa aatggcaaaa aataatgcag ttgctggatt taatgcactt 1441 aatggcgtag aattaaacct ttttaccact gacgaactta aggcaattca ctatgcaacc 1501 atggaagttc tgatggaccc aggaattcag gtttctgacc cagaggcaag gcagatcttc 1561 aaggaaaatg gctgcgaagt taatgaaaag acaaacgttg taaagatccc tgaatatctt 1621 gtaagaaaag ctcttcagct cgctccctcc agatttgtac tctggggccg tgataaaaaa 1681 ttcaacactg tacaggaatg tggtggtaaa gtacactgga cctgtttcgg taccggtgtt 1741 aaggtctgca aataccagga tggcaagtat gtgacagtcg actccgtcga aaaagatatt 1801 gcagacatcg caaaactctg tgactgggca gaaaacattg actatttctc actgccagtc 1861 tctgcaaggg acattgcagg tcagggtgca caggatgtcc acgaaacact tacccctctt 1921 gcaaacaccg caaaacactt ccaccacatt gaccctgttg gcgaaaacgt tgagtactat 1981 cgggacattg taaaggccta ttacggtggg gacgaggaag aagcaagaaa gaaacctatt 2041 ttctccatgc tgctctgccc taccagccca cttgagctca gtgtaaatgc ctgtcaggtc 2101 atcatcaagg gtgcccgctt tggaattcct gtaaatgtcc tgagtatggc aatgtcaggt 2161 ggttcttctc ctgtatatct tgcaggcact cttgtaaccc acaatgccga agttctttca 2221 ggaatagtac ttgcccagct gacagtacct ggagctaaag tctggtatgg aagttccaca 2281 actaccttcg acctcaagaa aggtacagca cctgttggat ctcctgaact cggtcttatc 2341 agtgctgcag ttgcaaagct tgcccagttc tacggccttc cctcctatgt ggcaggttcc 2401 tagtctgatg ccaaggttcc ggacgaccag gcaggccacg aaaaaactat gaccactctt 2461 ctgcctgctc ttgccggtgc caacaccatc tacggagctg gaatgcttga gctcggaatg 2521 acattctcaa tggaacagct tgttattgac aatgatatct tcagtatggt taagaaagcc 2581 atgcagggaa ttcctgtctc ggaagaaact cttgcagtcg aatctatcca gaaagtaggc 2641 atcggaaaca atttcctggc ccttaaacag accaggcagc ttgtggatta cccgtccaac 2701 ccaatgctcc ttgacagaca catgtttgga gactgggctg cagcaggctc aaaagacctt 2761 gcaacagttg cacacgaaaa agtagaggac gttcttaaaa accaccaggt aaccccaatc 2821 gatgcagaca ttttcaagga catgcaagca attgtggaca aggctgacaa agccttcaga 2881 ggcatgtaat taatattcag atattcagga gatctaaaaa tggcaaacaa agaagaaatc 2941 attgcaaaag caaaagaagc aatcaccgat tttgacgatg aacttgcaga agaagtagca 3001 aatgaagccc ttgctgcagg aattgacccc gtagagctta ttgagaaagg ctttaccgca 3061 ggcatggaag aagtaggaga aaaattcggc cagggcgaac ttttcctgcc acatgtactt 3121 gcagctgctg aggcaatgaa ttccggtata aaagttatca cccctgaaat ggaaaagcgc 3181 aagtctcaaa ccaaaagcct cggaaccgtt gctatcggaa ccatcgaagg cgacatccac 3241 tccatcggaa aggatattgt agcttccatg cttaacattg caggtttcaa agtagtggac 3301 ctcggaagag atgtcccaat taacactttt gtagaaaaag taaaagaact caaaccccag 3361 gttgttgcat cctccgcact tatgacaacc acgatggtca accagatcca gattgaggaa 3421 cagctgaaag aagcaggtgt tcgcgaccag gtaaagacca tggtcggtgg cgctcctgtt 3481 acccaggact gggcagacaa gatcggcgca gatatctatg gtgaaagcgc taatgacgca 3541 gtcgccaagg taaaagcagc cctgaatgtc tgataaagca gccttaaaaa acggttttaa 3601 ctgcagaaaa tgtattcaag catacatttt ctgcaacact ctattctgtg catttttgtg 3661 agactgtagc tcagtatata gatagaacag ttcttgcaaa tcgtattgcc caagggaata 3721 ttacaaaaac tgctaaaaaa caacagttga atagaagttt gaacctaagt tgaaaaatat 3781 tcctgagata tgagacccgc aaaaggagct tttgggctgc actttcaggt tgtggagatg 3841 gactggagat tatgtcctga gggaactcca cagcgttctg aaaaatggag ggagttttcg 3901 tggatttgaa agctttaaaa aaacaggaaa gtaaaagaaa aatcagtaaa gggtacatgt 3961 gggccttttt ctgtgctata ttctggggaa tctggtattt gccaggaacc gttgtttggg 4021 ttcttaaccc ttttgatgaa atgagtagtg ctatcgctaa aacaagtgga gacggcatat 4081 ctcttgttgt gactgccgta ctgattactg cctttaatgc attggctgtc atgctggcac 4141 ttatggtctg gaacggagta cttggaaaat acggagaact cgtaaggact cttaaggaat 4201 tccatccctg ctccaagtgg ttcttccttg cttctatctt cggtggtcct atggcaattc 4261 taggttcttt cattgctatg ggctttatcg ggggatcatt tgcagctgtt gcaggccttc 4321 tttaccctgt agtgggttca attcttgctt actactggta tggagaaaag atttccaaga 4381 gggcggcaat cggtattgca gtcatcgttc tgggaggtat ttctatttac ggtggtggac 4441 tttttactga actttcttca ggtaacgtcc cctggatagg atatctcgga ggtttgatgg 4501 cggctgccgg ctggggcatt gaaggtgcaa ttgcaggcaa aggtcttgat attgcagaac 4561 cggatgccgg gttgactctt cgcttccttg gtgagaacat aatttggtgg atcatcattg 4621 tgccagttct tgcacttgtg ggctttccga tgtactcgtt tgcactgcag gcatttgatc 4681 ctttgaccat actgatcttg gtctttgcag gtatcacctt tggtttctgc tacgttacat 4741 ggtacaaatc ttttccactt ataggagttg ggagaggcca gggaattgga aacctctatg 4801 gactctgtgc tatcatcttc ttattcctct tctttggaga tgttccggat tggactatta 4861 ttataggcgg tgctctctgt atcgctggaa gtttcattat gtttactgaa gacgcaagtg 4921 aacttgaaac tctgagaggt gagtaaagtg agcaaaaatc gtcctatcaa gttcagaatc 4981 ctggaacttt tcctggacgg aaacgaacac tggaactatg aaatcgtttc gaagatccag 5041 gaagagtacg acatgaggag caatttccag agagacagca ttaactttga catcattgaa 5101 ctggcgtctg gaggaatgct gaaggatgtg gagcagaaag ttgacgaaaa cggaatctac 5161 aaaaaaggct ttctactgca caagtatatg ataacagatt tcggcagagt tcgaggctct 5221 gatgcctgct tcagatatgt ttgattcaat ggaggataaa aatatgacgc aaacagcagc 5281 ggctttagca gcttacaatg catattgggc aaattccttc ataccggaag caaagattct 5341 gtggatggtt ttgatcctta ttctggcgat aatagctctc tggcaagcca gaacctttgt 5401 atcccaattc tgaaccccag aatacagttt tctaaatctg aattaaaata aaaagaaagg 5461 agactaaaaa tggcaactga atacgctttg cgtatgggag acggtaaacg tgtctacctt 5521 accaaagaaa aaattgtgtc tgaaattgaa gccgggactg cagatgctgc cgatctcggt 5581 gaaattccgg ccctgagcgc caatgaaatg gacaagcttg cagagatcct catgatgccc 5641 ggcaagactg ttagtgtcga acagggtatg gaaattcctg taacacacga catcggtacc 5701 atcaggcttg acggtgacca gggcaacagc ggtgttggta ttccttccag ccgtttagtt 5761 ggctgcatga cgcacgagcg tgcttttggt gctgacacaa tggaactcgg gcacattgat 5821 tacagcttca agcctgtcaa acctgttgta tcaaacgagt gccaggcaat ggaagtctgc 5881 cagcagaaca tggttattcc gttgttttac ggcgcaatgc caaacatggg attgtattat 5941 actcctgatg gacctttcga gaaccctggc gacctcatga aggctttcaa gattcaggaa 6001 gcctgggaat caatggaaca tgctgcagaa cacctgacaa gagacactgt ctgggttatg 6061 cagaaactct ttgcctccgg tgccgatggg gtcaacttcg acacaaccgg tgcagcaggc 6121 gacggtgaca tgtatggtac actccatgcg attgaagccc tcagaaaaga gttccctgat 6181 atgtacattg aagcaggcat ggctggagaa tgcgtacttg gtatgcatgg aaaccttcag 6241 tacgacggag taacccttgc aggtctctgg cctcaccagc aggctcctct tgttgcaaag 6301 gctggagcaa atgttttcgg accagtatgc aacaccaata ccagcaagac ctcagcctgg 6361 aaccttgccc gtgcagttac tttcatgaag gcagctgtcg aagcatctcc aataccatgt 6421 cacgttgaca tgggaatggg tgtgggcgga ataccgatgc ttgaaacgcc tccaattgat 6481 gcagttacaa gggcgagcaa agcaatggta gaaattgcag gcgtagacgg catatagatc 6541 ggggtcggcg accctctggg tatgccgatc gcacacataa tggcttcagg tatgacagga 6601 atgagagctg caggagacct tgttgcaaga atggagttct caaagaatat gcgcataggc 6661 gaggcaaaag aatacgtagc aaagaaactc ggggtcgaca agatggacct cgtagacgag 6721 cacgttatgc gcgaactgcg tgaagaactc gatattggta ttataacgtc agtaccaggc 6781 gctgcaaaag gtattgctgc aaagatgaac atcgaaaagt tgcttgatat taagattaat 6841 tcctgcaatc tcttcagaaa gcagatagct taaggaaata attcaggtag aagcctgaat 6901 gttcctcgac agcagggata aacaagatca agattgaaca aacttggacc tgccaaaaaa 6961 cagcagtaaa ataactataa actcttcagg caggtccgaa aagttcttcc ttataaaata 7021 gcgaccacct ttcctgctgt cattttctat ttttttgaga gtttacttat tatcctgata 7081 catccatcga atcttcaact gtaacctgct cttccttgta gctattgctg tatctggcaa 7141 ggtatttgca cctggagaaa acttagtatt tacaatctta ataacc //