LOCUS       AF046875                 768 bp    DNA     linear   BCT 25-JUL-2016
DEFINITION  Methanosarcina barkeri monoethylamine methyltransferase (mtmB)
            gene, partial cds.
ACCESSION   AF046875
VERSION     AF046875.1
KEYWORDS    .
SOURCE      Methanosarcina barkeri
  ORGANISM  Methanosarcina barkeri
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanosarcinales; Methanosarcinaceae; Methanosarcina.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Burke,S.A. and Krzycki,J.A.
  TITLE     Reconstitution of Monomethylamine:Coenzyme M methyl transfer with a
            corrinoid protein and two methyltransferases purified from
            Methanosarcina barkeri
  JOURNAL   J. Biol. Chem. 272 (26), 16570-16577 (1997)
   PUBMED   9195968
REFERENCE   2  (bases 1 to 768)
  AUTHORS   Burke,S.A., Lo,S.L. and Krzycki,J.A.
  TITLE     Clustered genes encoding the methyltransferases of methanogenesis
            from monomethylamine
  JOURNAL   J. Bacteriol. 180 (13), 3432-3440 (1998)
   PUBMED   9642198
REFERENCE   3  (bases 1 to 768)
  AUTHORS   Burke,S.A. and Krzycki,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-1998) Microbiology, Ohio State University, 484
            West 12th Avenue, Columbus, OH 43210, USA
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Methanosarcina barkeri"
                     /mol_type="genomic DNA"
                     /strain="NIH"
                     /db_xref="taxon:2208"
     gene            10..>768
                     /gene="mtmB"
     CDS             10..>768
                     /gene="mtmB"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="stop codon is read through by an undetermined
                     mechanism"
                     /codon_start=1
                     /transl_except=(pos:613..615,aa:OTHER)
                     /transl_table=11
                     /product="monoethylamine methyltransferase"
                     /protein_id="AAC38637.1"
                     /translation="MTFRKSFDCYDFYDRAKVGEKCTQDDWDLMKIPMKSMELKQKYG
                     LDFKGEFIPTDKDMMEKLFKAGFEMLLECGIYCTDTHRIVKYTEDEIWDAINNVQKEF
                     VLGTGRDAVNVRKRSVGDKAKPIVQGGPTGSPISEDVFMPVHMSYALEKEVDTIVNGV
                     MTSVRGKSPIPKSPYEVLAAKTETRLIKNACAMAGRPGMGVXGPETSLSAQGNISADC
                     AGGMTCTDSHEVSQLNELKIDLDAISVIAHYKGNS"
BASE COUNT          236 a          164 c          196 g          172 t
ORIGIN      
        1 aaaaagacaa tgacatttag aaaatcattt gactgctatg atttctacga cagagctaaa
       61 gtaggagaaa agtgtaccca ggacgactgg gaccttatga agatcccaat gaagtcaatg
      121 gaactcaagc agaagtacgg gcttgacttc aagggagagt ttatcccgac agataaggat
      181 atgatggaaa aactcttcaa agccggcttt gaaatgctcc ttgagtgcgg aatttactgt
      241 accgataccc acagaatcgt aaagtacact gaagacgaaa tctgggatgc aattaacaac
      301 gtgcagaaag aattcgtgct cggaactggc agggatgccg tcaatgtaag aaagagaagt
      361 gttggtgaca aggcaaagcc aatcgtacag ggtgggccta ccgggtctcc aatttccgaa
      421 gatgtgttta tgcctgttca catgagctat gccctggaaa aggaagtaga cacgattgtt
      481 aacggtgtca tgacctctgt acgtgggaaa tcccctattc caaaaagtcc ttatgaggtc
      541 cttgctgcaa agaccgaaac aaggctaatt aagaatgctt gcgccatggc gggtaggcct
      601 ggcatgggtg tctagggccc agagacctcc ctgtccgctc agggaaacat ttccgctgac
      661 tgtgccggcg gaatgacctg tacggacagc cacgaggtct cgcaactaaa cgaactcaag
      721 attgatcttg atgcaatttc agttattgct cactacaaag ggaacagc
//