LOCUS       AF009416                2340 bp    DNA     linear   PLN 16-MAR-1998
DEFINITION  Myrothecium roridum trichodiene synthase (TRI5) gene, complete cds.
ACCESSION   AF009416
VERSION     AF009416.1
KEYWORDS    .
SOURCE      Paramyrothecium roridum
  ORGANISM  Paramyrothecium roridum
            Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
            Sordariomycetes; Hypocreomycetidae; Hypocreales; Stachybotryaceae;
            Paramyrothecium.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Trapp,S.C., Hohn,T.M., McCormick,S. and Jarvis,B.B.
  TITLE     Characterization of the gene cluster for biosynthesis of
            macrocyclic trichothecenes in Myrothecium roridum
  JOURNAL   Mol. Gen. Genet. 257 (4), 421-432 (1998)
   PUBMED   9529523
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Hohn,T.M. and Trapp,S.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUN-1997) Mycotoxin Research Unit, National Center
            for Agricultural Utilization Research, USDA/ARS, 1815 N. University
            St., Peoria, IL 61604, USA
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Paramyrothecium roridum"
                     /mol_type="genomic DNA"
                     /strain="ATCC 52485"
                     /db_xref="taxon:1859971"
     gene            769..1984
                     /gene="TRI5"
     mRNA            join(<769..1237,1296..>1984)
                     /gene="TRI5"
                     /product="trichodiene synthase"
     CDS             join(769..1237,1296..1984)
                     /gene="TRI5"
                     /function="catalyzes FPP cyclization"
                     /note="terpene synthase"
                     /codon_start=1
                     /product="trichodiene synthase"
                     /protein_id="AAC49957.1"
                     /translation="MDEFPTEYFLGTAVRLLENVKYRDSNYTREERIENLSYANNKAA
                     AHFAQERQQRILKVNPKRLEASLRTIVGMVVYSWVKVSKELMADLSIHYTYTLILDDS
                     EDDPHNNMLTFFDDLQAGREQKHPWWMLVNEHFPNVLRHFGPFCSLNLIRSTLDFFEG
                     CWIEQYNFHGFPGSYDFPGFLRRMNGLGHCVGGSLWPKELFDEQKHFLEITSAVAQME
                     NWMVWVNDLMSFYKEFDDPRDQTSLVKNYVVCDEISLTQALEKLTVDTLTSSEQMMEV
                     FSDKDAKLMETIECFMHGYITWHLCDHRYRLKEVYEGTMHIETEDAIKFRKFYGQAAK
                     VGAIEHEEWAFPTVAERIEVRLAEEKAAKDGQAVLTSAEPAVPQAAQEVLA"
     exon            769..1237
                     /gene="TRI5"
     intron          1238..1295
                     /gene="TRI5"
     exon            1296..1984
                     /gene="TRI5"
BASE COUNT          549 a          620 c          578 g          593 t
ORIGIN      
        1 aagcttactg cccgagcttt gctggccatg ccgctagagc acaaaacttg ccacgaggcc
       61 tgtcatagga gaataagcca agacaaggcc tgagcgagcc gcttgaggac cgtctagcgt
      121 ggcttccgtc cccttcctct cggatatttc tgtaagtcga gcccggatgg attgtcgcgt
      181 acaggtcaac ttgtttctac ttttggctct tttcttcctt tttgcatctg aatttgccct
      241 tataacaaat catcctgctc tccagtagag ctatcaatgc ttcagttcca ctcccgcgcc
      301 tgcatgtggt aggcctgatg cgccccgtcg ggtgataagc accgaggatg gcaccgagcg
      361 cgtcgcaggt tctagggcca cgctgccacg attggttagt gccgaaaagg acgagggtcg
      421 gggaatttga ttcgactcgc ccgttcacgg cttacctaat gatgctctcc ttgaatttgc
      481 ttcctttcaa catccagagt aacctatacg gccctatacg gcgcgttttg agaggcccta
      541 gaatttgttc acgatggcaa tgctggatag ccgacgagga gagtctcctt agcaatgtta
      601 tttatgtctc atgttgagcc cggatgagag ggatagacag gaagatcaga cagacaggtt
      661 catcaatcaa cagttaagaa gtctcgactt tcactttctt ccaggaactg cctttttgtc
      721 tactttaatc tcccatcaaa caaatctact tcctcaaaat ctcccaaaat ggacgagttc
      781 cccaccgagt acttccttgg caccgccgtc cggctgctgg agaatgtcaa gtacagagac
      841 agcaactaca cgagagagga gcgtattgag aacctttcgt acgccaacaa caaggctgct
      901 gcccatttcg cgcaggagcg tcagcagagg atcctgaagg tgaaccccaa gaggctggag
      961 gcttcccttc gaaccattgt gggcatggtc gtgtactctt gggtcaaggt gtccaaggag
     1021 ctcatggccg acctcagcat ccactacacc tacactctca ttctggacga cagcgaggat
     1081 gacccacaca acaacatgtt gaccttcttc gacgatcttc aagccggtcg tgagcagaag
     1141 cacccctggt ggatgcttgt caacgagcac ttccccaacg ttctgaggca tttcggcccc
     1201 ttctgctcct tgaaccttat ccgcagcacc ctcgattgta agtttcaaaa agtatggact
     1261 cgtgtaaggt ctataatctg acatgtattg gctagtcttc gagggttgct ggatcgagca
     1321 gtacaacttc cacggcttcc ccggctctta tgacttcccc ggcttcctcc gtcgcatgaa
     1381 cggtcttggc cactgtgttg gaggatctct gtggcccaag gagcttttcg acgagcagaa
     1441 gcacttcctt gagattacca gcgccgttgc tcagatggag aactggatgg tgtgggttaa
     1501 cgacttgatg tctttctaca aggagtttga tgaccctcgt gaccagacca gcctggtcaa
     1561 gaattatgtt gtctgcgacg agatctctct tacccaggcc ctggagaagc tcaccgtcga
     1621 caccctgacc tcatctgagc agatgatgga ggtattctcc gacaaggatg ccaagcttat
     1681 ggagacaatc gagtgcttta tgcacggcta catcacctgg catctgtgcg accaccgcta
     1741 caggctcaag gaggtttacg agggcaccat gcacattgag actgaggatg caatcaagtt
     1801 ccgcaaattc tatggtcagg ctgctaaggt cggtgccatc gagcacgagg agtgggcttt
     1861 ccccactgtc gccgagcgaa ttgaggttcg ccttgcggag gagaaggccg caaaagacgg
     1921 acaggccgtg cttaccagcg ccgagccggc cgtcccccaa gctgcccagg aggttctcgc
     1981 ttagagccgc agctgttgga tattgaaaac ctttgaggac ttgcacttaa gcattccttg
     2041 agcttctgga catcttatta gaataatgtt tttcgggact ctggcgtaga attaggttta
     2101 tgaattgaat agacgataca tttgcattag accaaagatc catgcagtgt cactcagtga
     2161 acccttaaaa cgaaagtgat acctttacaa aactatattg cgctcttcta aagtgtcctt
     2221 cgactagggt acaagtcata caatgggtcg tctcaccagg cttcacatgt cgaaacgcaa
     2281 cgaattagtc tttgtattgt cacgctcttc cgcggaatct gtctttagta gtccattctt
//