LOCUS AF007224 4016 bp DNA linear PLN 07-FEB-2003 DEFINITION Pleurotus eryngii versatile peroxidase VPL2 precursor (vpl) gene, vpl2 allele, complete cds. ACCESSION AF007224 VERSION AF007224.1 KEYWORDS . SOURCE Pleurotus eryngii ORGANISM Pleurotus eryngii Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Agaricales; Pleurotineae; Pleurotaceae; Pleurotus. REFERENCE 1 (bases 1 to 4016) AUTHORS Martinez,M.J., Bockle,B., Camarero,S., Guillen,F. and Martinez,A.T. TITLE MnP isoenzymes produced by Pleurotus species in liquid culture and during wheat straw solid-state fermentation JOURNAL (in) Jeffries,T.W. and Viikari,L. (Eds.); ENZYMES FOR PULP AND PAPER PROCESSING, ACS SYMP. SER. 655: 183-196; (1996) REFERENCE 2 (bases 1 to 4016) AUTHORS Ruiz-Duenas,F.J., Martinez,M.J. and Martinez,A.T. TITLE Molecular characterization of a novel peroxidase isolated from the ligninolytic fungus Pleurotus eryngii JOURNAL Mol. Microbiol. 31 (1), 223-235 (1999) PUBMED 9987124 REFERENCE 3 (bases 1 to 4016) AUTHORS Ruiz-Duenas,F.J., Martinez,M.J. and Martinez,A.T. TITLE Direct Submission JOURNAL Submitted (07-JUN-1997) Department of Molecular Microbiology, Centro de Investigaciones Biologicas (CIB), CSIC, Velazquez 144, Madrid E-28006, Spain FEATURES Location/Qualifiers source 1..4016 /organism="Pleurotus eryngii" /mol_type="genomic DNA" /strain="ATCC 90787; CBS 613.91; IJFM A169" /db_xref="ATCC:90787" /db_xref="taxon:5323" /cell_type="hyphae of dikaryotic culture obtained from a mycelium sample collected from the cap" /note="from liquid culture" gene 230..2888 /gene="vpl" /allele="vpl2" /note="probably an allelic form of vpl1" protein_bind 230..236 /gene="vpl" /allele="vpl2" /bound_moiety="AP1" misc_feature 308..314 /gene="vpl" /allele="vpl2" /note="metal response element" protein_bind 457..462 /gene="vpl" /allele="vpl2" /bound_moiety="CreA" protein_bind 477..482 /gene="vpl" /allele="vpl2" /bound_moiety="SP1" misc_feature 493..501 /gene="vpl" /allele="vpl2" /note="heat shock element" regulatory 503..507 /regulatory_class="CAAT_signal" /gene="vpl" /allele="vpl2" protein_bind 646..653 /gene="vpl" /allele="vpl2" /bound_moiety="AP2" protein_bind 703..708 /gene="vpl" /allele="vpl2" /bound_moiety="CreA" regulatory 736..740 /regulatory_class="CAAT_signal" /gene="vpl" /allele="vpl2" regulatory 784..789 /regulatory_class="TATA_box" /gene="vpl" /allele="vpl2" mRNA join(840..908,964..1100,1156..1214,1268..1402,1453..1574, 1631..1706,1759..1795,1854..1861,1910..1990,2045..2114, 2173..2182,2230..2265,2330..2502,2556..2595,2661..2685, 2743..2888) /gene="vpl" /allele="vpl2" /product="versatile peroxidase VPL2 precursor" CDS join(845..908,964..1100,1156..1214,1268..1402,1453..1574, 1631..1706,1759..1795,1854..1861,1910..1990,2045..2114, 2173..2182,2230..2265,2330..2502,2556..2595,2661..2685, 2743..2755) /gene="vpl" /allele="vpl2" /note="encoded isoenzyme confirmed by N-terminal amino-acid sequencing of the different isoenzymes; allele vpl1 sequence deposited as GenBank Accession Number AF007223" /codon_start=1 /product="versatile peroxidase VPL2 precursor" /protein_id="AAD01404.1" /translation="MSFKTLSALALALGAAVQFASAAVPLVQKRATCDDGRTTANAAC CILFPILDDIQENLFDGAQCGEEVHESLRLTFHDAIGFSPTLGGGGADGSIIAFDTIE TNFPANAGIDEIVSAQKPFVAKHNISAGDFIQFAGAVGVSNCPGGVRIPFFLGRPDAV AASPDHLVPEPFDSVDSILARMGDAGFSPVEVVWLLASHSIAAADKVDPSIPGTPFDS TPGVFDSQFFIETQLKGRLFPGTADNKGEAQSPLQGEIRLQSDHLLARDPQTACEWQS MVNNQPKIQNRFAATMSKMALLGQDKTKLIDCSDVIPTPPALVGAAHLPAGFSLSDVE QACAATPFPALTADPGPVTSVPPVPGS" sig_peptide join(845..908,964..989) /gene="vpl" /allele="vpl2" mat_peptide join(990..1100,1156..1214,1268..1402,1453..1574, 1631..1706,1759..1795,1854..1861,1910..1990,2045..2114, 2173..2182,2230..2265,2330..2502,2556..2595,2661..2685, 2743..2752) /gene="vpl" /allele="vpl2" /product="versatile peroxidase VPL2" /note="for molecular model of allelic variant vpl1 see Brookhaven PDB entry 1A20" regulatory 2871..2876 /regulatory_class="polyA_signal_sequence" /gene="vpl" /allele="vpl2" polyA_site 2888 /gene="vpl" /allele="vpl2" BASE COUNT 946 a 1032 c 927 g 1111 t ORIGIN 1 tcgacctgta taaacaatcg gtgaatcgct ttatcaaggt ctgataaccg aaggcatcaa 61 acatgccgaa ttcatatcca acttccgaag gctgcttggc gtctcgcccg ttaccgtaca 121 gctggaatgc gttgacatcg tgttacaaag gtcgaatcac agctcgcgta ttagagctac 181 tgcgatcttt gaaggcgtac gcactatcct cggagcttgg ccccatttgt gagtgagatg 241 atatggtgcc gaactttgcg ccattgctga cgactactag tattctcaga gtcgaacctg 301 gctgctgtgc acgccgcaat gagaggtcag cgacccagac cccgttgaat cttgagaggt 361 aaggaaagaa gatggccacc ctcggataat ggacatcctc gattgtgcaa tcgcaattcg 421 tgcatttgtg cattgtctac atggcgaact gctaagccgg ggaagaattc tgatagccgc 481 ccctttgttg ttgaaatttt caattggcat cagggtttgt tagcatcttt gcccgtagca 541 ctgggacccg tcgcgtcggt cgcgctctcg gtcaaatgta tcgcatggat cccgctcgac 601 ctttaggatg aggtgggaat acacggcctt tccttgcagg ttatgcccat gcctctatgg 661 caaacctctt cgtcgcgtcc acacaccaaa gcaatggacc ctctccgctg acatttcatg 721 gaaacgaggg atggtattgg tcgagtcatc gatatactga ctctttactg tgcgtttttt 781 cggtataaag ggtcgcctac taactttctt tcttccccaa gatccagtct tacaacccac 841 tgcaatgtct ttcaagacgc tctccgctct cgcgcttgcg ctcggcgccg ctgtccagtt 901 cgcgagtggt gggcttcgtc tctgaccaca atgtcgaggc aaccctgctg actggtattt 961 tagctgctgt gcctctcgtc cagaaacgcg caacttgcga cgacggacgc accaccgcaa 1021 atgctgcatg ttgcattctg ttccccatcc tcgatgacat ccaagaaaac ctcttcgacg 1081 gtgcccagtg tggagaagag gtgttatacc cattgtccta atgcagaacg gctattaatt 1141 cacgcgcgta cacaggtgca cgagtccctt cgtttgactt tccacgatgc aatcggtttc 1201 tctcctactt tagggtaagg taacgatcac cactcgtgtt gtctaatttc taacccatgc 1261 acaacagcgg aggaggagct gacggttcca tcatcgcgtt cgacaccatt gagactaatt 1321 tccccgccaa tgctggcatc gatgaaatcg tcagtgctca gaagccattc gtggctaaac 1381 acaacatctc cgccggcgac ttgtaagcag ttcaagccaa aggttaaagt gacccttaca 1441 aatcaattct agcattcaat ttgctggcgc cgttggagtc tccaactgcc ctggtggtgt 1501 caggattcct ttcttcttgg gtcgcccgga tgccgtggcc gcctccccgg accacctcgt 1561 gccagagcct tttggtacgt ttggatatgg taaaattatc aacgagaact gagggtgtgg 1621 gctcgcatag attctgttga ctccattctt gccagaatgg gtgacgcagg cttcagtccc 1681 gtcgaggttg tttggctcct ggcttcgtga gtgcatggag atatgtaagt accaccatcg 1741 atctaatttg ttgcctaggc actccattgc cgctgccgac aaggttgacc catcggtaag 1801 tcgagctgca ttggcaagtt tgttgtgaag gctaatacct gataattgtc tagattcctg 1861 ggtaagagcg aacatccact gacgcggcat gctactgact tcatgtcaga acgccattcg 1921 attcaacccc cggagttttt gattctcaat tcttcatcga aacgcaactt aaaggcagac 1981 tcttcccagg gtaagcgatt ccatttcttc tcaccacgat atgacccatc atgtttgtgt 2041 ttagcactgc tgacaacaag ggagaagccc aatctccatt gcaaggagag atcaggcttc 2101 agtccgatca cttggtgaga ccattgtagt tcattattct atcagtaata ctgacaaata 2161 tccactggat agttggctag aggtacgatt cttccttgca gccttgttaa tgctcaccgc 2221 ctcccaaaga cccccagact gcctgcgaat ggcagtccat ggttagtgag taaaatattc 2281 cctttcatac tttataacat agagactgac tacatcgccc tactaccaga caaccaaccg 2341 aagattcaga accgtttcgc tgctaccatg tcgaagatgg ctcttcttgg ccaagacaag 2401 accaaattga ttgactgttc cgatgttatc cccacccctc ctgcccttgt cggagcggcc 2461 cacttaccgg cgggattttc tcttagcgat gtagagcaag cggtacgtgc acatgtttcc 2521 ctggataaat gaggggtctc accatctgtg atcagtgcgc cgcgacgcct ttccctgctc 2581 ttactgctga cccaggtgaa tacatgcggt gttaacattt tatcaccggg ccatttgaac 2641 tgactgatat ctctcttcag gcccagtaac ctccgtccct cccgtgtaag ttttcagtca 2701 cccagtatgt tatggcgcgc tcattgtcta cgtctcctac agccctggat cgtaaatgct 2761 tcgatacctg aatatgctcg ttctgctgct atgaacttcc aatttttgcc attgggtctg 2821 tattcgattc tagatgtttg tgatatcaac tgtgtataaa tgatcttttg aaatatactt 2881 ttttctgcgg agtatgttcg ggtgtctacg accattgccc aacgctcttg actaaaatct 2941 tctcatggca gttgtgaatt taccggggct cttcgtccag tatcgtgaca ctgttttgag 3001 ggtccgagaa tctacaattt aaattacacc ttctcaacta ttcttgttct tcaccagtaa 3061 gtaatcgaat agatgtacat aaggagaatg gagatgcact tccgctccca atacgtatgg 3121 aggaacggaa agccggcagc tagtaataga cggtatgcat ttatgctaca aagggacggt 3181 gtgtgattgg attatccaac aaaaggtcga taatttgcca agcttcgtct tgagtaacct 3241 tggaaaatcg tgcttccttc attcctgctg ctttccaaag gcgagcgagt tcagcgtgtc 3301 tagctcgaaa ctgcccctcc ggttggtcat ccagcggcca catcgaagtc acgccaaccg 3361 ttttccttgc ccaatcctca ccagcaaggg ccttgaaggt accttcgtag gtggcgattg 3421 gttggattcg gttgtctccg atattctgca agtagataat accatccacg cgtctcttag 3481 ggcatctaaa tcattcatgg ttagtacatc gcgtgaattg ctctcgagtt gaatgcctag 3541 cttacttagc atgaacttcg gcaatctgaa tttggtccat actttgcagc tcaggtgtgt 3601 cgataaagat gatctcgctc tcccgtgtga gtagctcgta agggatagcc tcgacctcta 3661 ctgtatttgg ttccaatgtc agtttatttg ggtcagaggg cggcataggc ctcccagtag 3721 ctgagttgat aaactgaaaa tacatgataa acaactcagt aaaggccgga ctacccgttg 3781 aatagtaatc accgtggacc gcccggatcc tgacgctccc aaaagtctat cgagtatttt 3841 tagctggctt tagaagcgca ttggtgttca gaacgtacgc tattacgcga acctagcaaa 3901 cattgagata tattattgag gtttacataa aagtattgca taaagcgcac gcaccgaatt 3961 atacatccga tcgattatgc gccaggcgtc tgcgtagtcg ccataaaatc ggtcga //