LOCUS AB812079 16810 bp DNA linear BCT 13-MAY-2015 DEFINITION Escherichia coli genes for O-antigen biosynthetic locus, partial sequence, strain: 99-1287. ACCESSION AB812079 VERSION AB812079.1 KEYWORDS . SOURCE Escherichia coli ORGANISM Escherichia coli Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. REFERENCE 1 (bases 1 to 16810) AUTHORS Iguchi,A., Iyoda,S. and Ohnishi,M. TITLE Direct Submission JOURNAL Submitted (02-APR-2013) to the DDBJ/EMBL/GenBank databases. Contact:Atsushi Iguchi University of Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1, Miyazaki, Miyazaki 889-2192, Japan REFERENCE 2 AUTHORS Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M., Hayashi,T. and Thomson,N.R. TITLE A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster JOURNAL DNA Res. 22, 101-107 (2015) REMARK DOI:10.1093/dnares/dsu043 COMMENT FEATURES Location/Qualifiers source 1..16810 /db_xref="taxon:562" /mol_type="genomic DNA" /note="serogroup:O182" /organism="Escherichia coli" /strain="99-1287" CDS <1..905 /codon_start=3 /gene="wcaM" /product="colanic acid biosynthesis protein" /protein_id="BAQ02007.1" /transl_table=11 /translation="YAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILIS DHVIERINCTNGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVEN GKHFVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIK GKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKP QHLFMRNINVMQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSV DIDRINHHIVNVEKINFRLPERRE" CDS 1080..1973 /codon_start=1 /gene="galF" /note="O182_01" /product="UTP-glucose-1-phosphate uridylyltransferase" /protein_id="BAQ02008.1" /transl_table=11 /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY GLRNLKEGAKFRKGIEKLLSE" CDS 2284..3114 /codon_start=1 /note="O182_02" /product="putative acetyltransferase" /protein_id="BAQ02009.1" /transl_table=11 /translation="MYLRKVDMININMRKNNASREYLDICWDTFFKSKNRGLDLVAHF PWLTSGSSSFISYEVEKNNQIVGGLIVLERDIRNLKIGLVGLVCVKKECRGQGIFKAL MRKMLISCKESHFSALYLWSSKHSIYEKFDFELHDKNILVKIKVINDGDFGKPILNNY GDVNIIHRTDLAVPPFASGIYELKTECGSIVYCESNGNKYIINHENTVENVILEFGKF NKGEFFLNTIIDRKNNILSMKDYFDVEIIRQNIQMVNLIDKASIDKVYKINFLVNERI " CDS 3117..4214 /codon_start=1 /note="O182_03" /product="putative aminotransferase" /protein_id="BAQ02010.1" /transl_table=11 /translation="MIPIVKVFMPPKDVLMPALEQVLYSGMVGEGKHVYTFEDKFKKL FGINNAIAVSSGTAALHMALILAGIKKGDEVITTSMTAEPTNTSILQAGGVPVFADVD KNTGCLDPDSIIEMISDKTKAILLVHYAGYLVDVPELKEKLRRKINRKIKIIEDCAHA LGATIDGRMVGTFGDYGIFSFQAIKHMTTIDGGFLVIKDTSQTKLAKKMRWFGMEKGV ERTSLDLDVLGFKYNMNNVTGVIGEIQLDYVKDNISRHQKNAQFYNMMLNDITGIEVV ESRQSHSPSYWLYTLLCDDSDDIINKLNEINVAASKLHRPNHYHSLLSQCRRASMENL DSFYKRLVHLPCGWWIDEITRSKIIEALRRG" CDS 4216..5367 /codon_start=1 /note="O182_04" /product="putative aminotransferase" /protein_id="BAQ02011.1" /transl_table=11 /translation="MINLFNVSFDHDIQKVVEDTLVSKAIASGAHVCQLESRLSDLFS EKYALTVNDMTNGITLLLDVFGLKKGDEVLVSPFSCLATTSPMALLGIKPVWVDIEPS TLAMNIKSIESNITKKTKAILVYHVAGYPSDIVEISKIAKKNNLLLIEDCNSAFGSKY RNKSLGSWGDATVLSFYPNRQVGSIDGGCVLFKDKNHYELAKLRRRYGVDFNDFRKVN GEINEAKDVETYGYSATINNVSAGIIMAKLPKYANEMLLTQKNVSKLNNVVMSLQNKY KGSIKLVTPLKDSCVNNWVYFVRVRNKESFMNEMKKKGVHVSSLHLRNDLYSCFHAER KHLTGVDIISDTLVAIPCGYWISEEDIDIIGKYIEQTLIFLVKNETSYN" CDS 5375..6718 /codon_start=1 /gene="wzx" /note="O182_05" /product="O-antigen flippase" /protein_id="BAQ02012.1" /transl_table=11 /translation="MLYFGKFGALAVGFFLMPAYSYYLGPASFSTVAFILSLVNAAVT LDFGMSTIIGRDSSDSTISNDKKYKQFLASQFLVIFFYLSVFSIVAIYSFLFQKGDLG VKISFLSVILIMSSLIQNISVSYLNGIKEFKISGSLIFLSVTLRGVISLLVIKYIDNN IISFMSAQSIIGLLFSLIFMLLPSRLDKEIKINNSWGGFFEPSIYIKFLLPLVKKGFP LLFMGVSATLVMQFDKIILSYYASDATLSAYYLAFTFSTIPILAIAGPIKQYFQPHIV SYLTQNNVLYRKNSIVFFWCLIVFVAVPSALGYSSLYQILSIWLGNNELIDKVDFFSH ILLPAFVLGAISYFPSVLLVVAEDYRFQSVFAIITSIIFVFLLFILAISSNVRFIPWL FIGYFCVVILFCSIRCFSLTKVNLCIFDMVKNSPFAILIIALSYWVGNIVSAMIF" CDS 6772..7782 /codon_start=1 /gene="fnlA" /note="O182_06" /product="UDP-N-acetylglucosamine 4,6-dehydratase" /protein_id="BAQ02013.1" /transl_table=11 /translation="MFKDKTLMITGGTGSFGNTVLKRFLNTDVKRIIVFSRDEKKQED MRIALANPKLKFIIGDTRDYESVYRAMNGVDYVFHAAALKQVPSCEFYPMEAVKTNVN GTENVLNAAIDCGVKRVVLLSTDKAVYPINAMGISKAMAEKVLIAKARRQDNDKTIMC ATRYGNVMASRGSVIPLFFEKIKLNKPLTVTDPNMTRFLMSLEDSVDLVLHAFKNGRQ GDIFVQKAPACTIGDLTEAIKQLCDSNIETKIIGTRHGEKLYESLVSREEMVKAEDMG RYYRIPADNRDLNYDKYIIKGQIDINKLDDYTSHNTERLNVEQIKALLLTLKYVQKEL GI" CDS 7834..8997 /codon_start=1 /note="O182_07" /product="putative glycosyltransferase" /protein_id="BAQ02014.1" /transl_table=11 /translation="MVYMIKLSILIPTKNRSEYLDDVLSSLLTWKRDDFEVIIQDNSD DLKTHNVVEKYRYDHRVKYFYVKEHLSVIDNFEYAVKNSSGKIITAIGDDDGILPIVM DVADWMLKNSVEAVLTNKAEYVWPDLISKYNSNYNNSKLKFNKNYSYDYXKINCIDEF LQVCRHGGTSMGRLPRIYYGLVCRESLDHVFNLTGQYFPGPSPDMANAASLSLVIKSF YYLNFPVFIAGSSSKSTAGLGLKRKHLGAISEIKHLPMNTMAEWNKNIPDFWSGPTIW AQSISSVIKKMEPTYDLNYEYLYARCFVFFPQKSKCIYLALRQYGISGKTVIKIIYWY VYNWKLRFVHLLPKLLAKIGKKDHNIITIDEQLKISQAVDSLLSFTQKSDLSK" CDS 8994..10220 /codon_start=1 /gene="wzy" /note="O182_08" /product="O-antigen polymerase" /protein_id="BAQ02015.1" /transl_table=11 /translation="MSENILSRLTLNSAQKCLLTGLIIILLGPYGGALGFALVVCGAG LEKGRIERYILCVFSFFIFTLCSVQGVFHSSNFNTISLFTALCTVMVLFLVDNNSLIL ILDKLIFVISVTVPIALIIAISQTLGVYPFEFRSNEVLGVMRFTFLFAEPSHYSILLS LCSLISLVKKTNLFTRFILLFGLLMTWSLSGFCIFTFGYIAYKFYTRGIIKYFIISLL LGSIITFLWFFKLQYSDFWLVAKIDSIIQLFSGNTVGASSALLRFNSIIIGPYFINEK INSDNFLLSFFGLGFGNLSDFVSVYYQSNFGLPDVYDANNIISNVIISSGIVGLVFYI LSFSYFTGLNLVNILFAAIILLCLSLFSGYAFGPFAILTLLLSRAFILLVINHDKARI NRCTYFNTWFENKTGS" CDS 10153..10938 /codon_start=1 /note="O182_09" /product="putative glycosyltransferase" /protein_id="BAQ02016.1" /transl_table=11 /translation="MTKHASIDVLISTHGSRIKQVPNILHDFKRNVRYIICHQNFELY EPNEILLNRTDVIYIRSTSNGVTKSRNILLDKADADLIYFCDDDVVLSENFDEIIINS HKKYSDDVILFNIRDENGELRKKYPNEVVKKTRFNILSVGTIEISLKNSPLLPKFPED MGAGTSLPVGDEAVFLSTFIKKGKSIRYIPNTIAIHPRESTGLEVTNGSIYSRGVALN RIYGIYAFLVAIFFLLRRRSLLKTDRGVLQGGILFFKGVLLGK" CDS 10951..11700 /codon_start=1 /note="O182_10" /product="putative glycosyltransferase" /protein_id="BAQ02017.1" /transl_table=11 /translation="MKISIVTATYNSWPYIKETYDSIANQSYKNWEWHVTDDCSSDYT YQYLKQLADRDCRIVIHRNEINSGAAVARNKSLSQCSGDYIAFIDSDDLWNPEKLESQ LKFMVDNQLDFSFTGYEVIDESGKSIKKYIDINQTQAISYEDMLKKKATLGCSTVMLR RLAFSELSMPDLRTGQDYALWLKLLKTGIRAVPYNKSLMKYRIVSSSISRNKYRKAKR QWQIYRELEKLSLVKSIYCFLFYAWRAIFRK" CDS 11835..13241 /codon_start=1 /gene="gnd" /note="O182_11" /product="6-phosphogluconate dehydrogenase" /protein_id="BAQ02018.1" /transl_table=11 /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY FKQIADDYQQALRDVVAYAVQNGIPVPTFSAAVAYYDSYRAAVLPANLIQAQRDYFGA HTYKRIDKEGVFHTEWLD" CDS 13490..14656 /codon_start=1 /gene="ugd" /note="O182_12" /product="UDP-glucose 6-dehydrogenase" /protein_id="BAQ02019.1" /transl_table=11 /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR DVTEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYDNLHPSRIVI GERSERAECFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII YEPVMKEDSFFNSRLERELATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD" CDS 14695..15780 /codon_start=1 /gene="wzz" /note="O182_13" /product="O-antigen chain length determinant protein" /protein_id="BAQ02020.1" /transl_table=11 /translation="MLLTEQFIIFITYPIAFTRIIAKLWRLKIISSVRVMMRVENNNV SGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPD VGQIAGYTNAMNVIYGPAVPKVSDIQASLIGRYSTAFSALAETLDNQEEAEKLTIEPT VKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVNEELEKDLKDNIALRMKNLQDSL KTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQQTQDVTQDTMFLLGSDALESMI KHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIHAYRYVMKPTLPIRRDSPKKAITL ILAVLLGGMVGAGIVLGRNALRNYNAK" CDS complement(15878..16489) /codon_start=1 /gene="hisI" /note="O182_14" /product="phosphoribosyl-AMP cyclohydrolase" /protein_id="BAQ02021.1" /transl_table=11 /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ" BASE COUNT 5074 a 2942 c 3578 g 5215 t ORIGIN 1 attacgccat cttacgccag ggatttcata accagattat cggtgccaac atcaccaact 61 gtaagttcag tgatttacag ggcgacgcca ttgaatggaa cgtggcaatt aacgacagtg 121 atattttgat ctctgaccat gtcatcgagc gcatcaactg taccaacggc aaaatcaact 181 ggggaatcgg cataggcctt gcggggagta cttatgataa taactacccg gaagaccagg 241 cagtgaaaaa ctttgtcgtg gcgaatatca cgggatcgga ttgtcggcag ttgatccatg 301 ttgaaaatgg taaacatttt gttattcgta atatcaaagc ccgcaatatc acgccggatt 361 tcagtaagaa agcaggtatt gataacgcca cagtcgctat ttacggttgt gacaatttcg 421 tgattgataa tattgaaatg attaatagcg ccgggatgtt aatcggctat ggggtaatta 481 aaggcaaata tctctcgata ccacaaaatt tccgagtgaa taatattcag ctggataata 541 cccaccttgc ttataaattg cgcggcatcc aaatctccgc cgggaatgct gtctcctttg 601 tggcactgac taacattgag atgaagcgtg cgtcgcttga gctacacaat aaaccgcaac 661 acctttttat gcgaaatatc aatgtgatgc aggaatcctc agttggacca gcattgagca 721 tgaacttcga catgcgcaaa gacgttcgcg gcgtctttat ggcgaaaaaa gaaacactgc 781 tgtctcttgc aaatgttcat gcggtgaatg aaagagggca aagctccgtc gatatcgaca 841 ggattaatca ccatattgtt aatgtggaaa agattaactt tagattgccg gaacggaggg 901 agtagatttg cgaccattcc tggaaaaatg gagccatact taggaacaat gctactgcaa 961 tccacaacga agcggcgtaa catcacaagt aattcagtaa tcaattcagg gtaattgatg 1021 ctggcgaaaa aaatcgaaca agctataatt cagcaaccat tttacaggtg gatgaaataa 1081 tgacgaattt aaaagcagtt attcctgtag cgggtcttgg gatgcatatg ttgcctgcca 1141 ctaaggcgat tcccaaagag atgctaccga tcgtcgacaa gccaatgatt cagtatattg 1201 ttgacgagat tgtggctgca gggatcaaag aaatcctcct ggtaactcat gcgtccaaga 1261 acgcggtcga aaaccacttc gacacctctt atgaattaga atctcttctt gagcagcgcg 1321 tgaagcgtca gctgctggcg gaagttcagt ccatctgccc gccgggcgtg accattatga 1381 acgtacgtca gggcgaacct ttaggtttag gtcactccat tttgtgtgca cgacccgcca 1441 ttggtgacaa cccatttgtc gtggtgctgc cagacgttgt gatcgacgac gccagtgccg 1501 atccgctgcg ttacaacctt gctgccatga ttgcgcgttt caacgaaacg ggccgcagcc 1561 aggtgctggc aaaacgtatg ccgggtgacc tctctgaata ctccgtcatt cagaccaaag 1621 aaccgctgga tcgcgaaggc aaagtcagcc gcattgttga atttatcgaa aaaccggatc 1681 agccgcagac gctggactca gacatcatgg ccgttggtcg ctatgtgctt tctgcagata 1741 tttggccgga acttgaacgc actcagccag gtgcatgggg acgtattcag ctgactgatg 1801 ccattgccga actggcgaaa aaacagtctg ttgatgccat gctgatgaca ggtgacagct 1861 atgactgcgg taaaaaaatg ggttatatgc aggcatttgt gaagtatgga ctacgcaacc 1921 tgaaagaagg ggcgaagttc cgtaaaggga ttgagaagct gttaagcgaa taatgaaaat 1981 ctgaccggat gtaacggttg ataaggaaat tataacggca gtgaagattc gtggcgaaag 2041 taatttgttg cgaatattcc tgccgttgtt ttatataaac aatcagaata acaacgagtt 2101 agcaatagga ttttagtcaa agttttccag gattttcctt gtttccagag cggattggta 2161 agacaattag cgtttgaatt tttcgagtta agcgcgagtg ggtaacgctc gtcacatcgt 2221 agacatgcat gcagtgctct ggtagctgta aagccagggg cggtagcgtg ttttatttac 2281 tgcatgtatt tgagaaaagt agacatgata aatattaata tgcgtaaaaa taacgcaagc 2341 cgagagtatt tagatatttg ttgggatact ttcttcaaaa gtaaaaatag aggcctcgat 2401 cttgtcgctc atttcccgtg gctaacttca gggtcgtcta gttttatttc ctatgaagtc 2461 gaaaaaaata atcaaatagt tggtggtctc attgttctag aaagggatat aaggaactta 2521 aaaattggac ttgtgggttt agtttgcgtc aaaaaagaat gccggggaca aggtattttt 2581 aaagccctta tgcgtaaaat gctgatatcg tgcaaagagt ctcatttttc ggcattatat 2641 ctttggtcgt caaagcatag tatttacgaa aagtttgatt ttgaacttca tgataaaaat 2701 atcttagtca aaatcaaggt gataaatgat ggtgattttg gaaaacccat attaaataat 2761 tatggagatg taaatatcat acaccgcaca gatttggctg ttccaccttt tgcatctggt 2821 atttatgaat taaaaactga atgtggctct attgtatatt gtgaaagcaa tggtaataaa 2881 tatataatta accacgaaaa tacagtcgag aatgttatac ttgaatttgg aaaattcaat 2941 aaaggtgagt tttttttaaa taccataatc gatcgaaaaa ataatatttt atctatgaaa 3001 gattatttcg atgtggaaat aataagacag aatatacaga tggttaattt aattgataaa 3061 gccagtattg acaaagtgta taaaattaat tttttagtaa atgaaaggat ttagtaatga 3121 ttccaattgt taaagttttt atgccaccta aagacgtttt gatgcctgct ttagaacagg 3181 ttctttacag tggaatggtc ggtgaaggta agcatgttta tacctttgaa gataaattta 3241 aaaagctctt tgggattaat aatgctattg ctgttagtag cggtactgcg gcattacaca 3301 tggcattaat cttggctggt ataaaaaaag gcgatgaagt tattaccacc agcatgacag 3361 cagaaccaac aaatacatca attttgcaag caggaggggt tcctgttttt gctgatgtag 3421 ataaaaacac aggatgtctt gatcccgatt ctattattga gatgataagc gataaaacta 3481 aagctatatt acttgtccat tatgcaggat atctggttga tgttcctgaa ctaaaggaaa 3541 aattaagaag aaaaataaat agaaaaatta agattattga agactgtgca cacgctttag 3601 gcgcgacaat tgatgggaga atggtaggaa ccttcggtga ctatggcata ttctcgtttc 3661 aggctatcaa acatatgaca acaatcgatg gtgggtttct tgtaattaag gatacctctc 3721 agacgaaatt ggctaaaaaa atgcgatggt ttggcatgga aaagggagtg gaaagaacat 3781 ctcttgacct agatgttctg ggatttaaat ataatatgaa taatgttact ggtgttatag 3841 gcgaaataca acttgattac gtaaaagata atatatctcg ccatcaaaaa aatgcccaat 3901 tttataatat gatgctgaat gatattacgg gcatagaggt agtagaatct aggcaaagcc 3961 actcaccgag ttactggctc tatactttat tatgtgatga ttctgatgat atcattaata 4021 aattgaatga aataaatgtg gctgcttcga aactacatag gccaaaccat tatcattcac 4081 ttctatcgca atgcagaagg gctagcatgg aaaatcttga ttcattctat aaaagattag 4141 ttcatttacc ttgtggttgg tggatcgacg aaataacacg tagcaaaatt attgaagcat 4201 tgagacgggg gtaatatgat taatttattc aatgtctcat ttgaccatga tattcagaaa 4261 gtggtagaag acactctggt ttctaaggct attgcatcag gtgcacacgt atgtcagcta 4321 gaaagtaggt tatctgattt attttcagaa aaatatgctc tgacagtaaa tgacatgact 4381 aatggtataa ctttattatt agatgtcttt ggacttaaga aaggagatga agttttagta 4441 tcacctttta gctgtttggc aacaacttca ccaatggcct tgttaggtat taaacctgta 4501 tgggttgata ttgaaccatc aactctggct atgaatatta aatcaataga aagtaacata 4561 acaaaaaaaa ctaaagcaat attggtatat cacgtggcag gttatcccag tgatattgtt 4621 gagatttcta aaattgcgaa aaaaaataac ctattgttaa ttgaagattg taattctgca 4681 tttggaagca agtatagaaa taaatcgctt ggttcttggg gggatgctac agttttatca 4741 ttttatccaa atagacaagt tggttctatt gatggaggat gtgtactttt taaggataaa 4801 aatcactatg aactggcgaa attaaggaga cgctatggag ttgattttaa tgatttccgt 4861 aaagtaaatg gagaaattaa tgaagctaaa gatgtagaga cttatggcta ttcagcaaca 4921 attaataacg ttagtgctgg aattattatg gccaaactac ctaagtatgc taatgaaatg 4981 ttgctcaccc aaaaaaatgt atcgaaattg aataatgttg ttatgtctct acaaaataaa 5041 tacaaaggaa gtattaagct ggtgacacct ttaaaggact catgcgttaa taattgggtt 5101 tattttgttc gtgtaaggaa taaggaatct ttcatgaatg aaatgaaaaa gaaaggagtg 5161 catgtatcgt ctttgcattt aaggaatgat ctgtattcat gttttcatgc agaaagaaaa 5221 catttaacag gggttgatat tatatcagat acactagttg caataccttg tgggtattgg 5281 atatcagaag aagacatcga tattattgga aaatatatag aacaaacatt aatatttttg 5341 gttaaaaatg aaacatcgta taattgattt ggggatgtta tattttggca agtttggcgc 5401 gttggcagtt ggtttttttc ttatgcctgc gtatagttat taccttggcc ctgcttcctt 5461 ttcaactgtc gcctttatcc tttcgttagt taatgcagcc gtcacattag attttggcat 5521 gagtactata attggaagag attcttctga tagtacaatt agtaatgaca agaagtataa 5581 acaattttta gcatcacaat ttcttgtgat atttttttat ttatctgtat tttccattgt 5641 tgctatatac tcttttcttt tccaaaaagg agatttgggg gtaaagataa gttttctgtc 5701 agttattctt atcatgtcat ctctaattca gaatatttcc gtatcttatc tgaatggaat 5761 taaagaattt aaaattagtg gttctcttat ttttttgtca gttacattac gaggggtaat 5821 aagtctttta gtaataaaat atattgataa taatattata tcatttatgt ctgctcagtc 5881 tataataggg cttttatttt ctttgatttt tatgctttta ccttctagac tggataaaga 5941 aataaaaata aataatagtt ggggtggttt ttttgaacct tcaatctata taaaattttt 6001 gctacctttg gtaaagaaag gtttcccttt gctttttatg ggagtatcgg ctacattggt 6061 tatgcagttt gataaaatta ttctttcata ttacgctagc gatgcaacgc tttcagccta 6121 ttacttagca tttactttca gcacaatacc tatacttgcg atagctggac ctattaaaca 6181 atattttcaa cctcatatag ttagttattt aactcaaaat aatgtgctgt acaggaaaaa 6241 tagcattgtc tttttttggt gcctgattgt ttttgttgca gtaccttcag cattaggata 6301 ttcatcgtta taccaaatat tatctatttg gcttggaaat aatgagttga ttgataaagt 6361 agactttttt tcacatatcc tgttgcctgc attcgttttg ggcgctataa gttattttcc 6421 gtctgtatta ttagtggttg cagaggatta tagatttcag tctgtatttg caattatcac 6481 gtctattatc tttgtttttt tattatttat acttgctata agtagtaatg ttcgtttcat 6541 accatggttg tttataggtt atttttgtgt ggttatttta ttttgcagta taagatgttt 6601 tagtttaact aaagtcaatt tatgtatatt tgatatggta aagaacagtc cttttgcaat 6661 tctaataatt gcgctgtcct attgggttgg taatattgta tcagcaatga ttttttaatg 6721 attgcttata ttatctatat atatcaatgt taattaaagc tgggattaat aatgtttaaa 6781 gataagactc taatgattac tggtggtact ggatcatttg ggaatactgt gctgaagcgc 6841 tttcttaata cagatgtaaa acgaattatt gtttttagtc gagatgagaa aaaacaagaa 6901 gatatgcgga ttgctctcgc aaacccaaaa ctgaaattta ttattggtga tactcgtgat 6961 tatgaatcag tataccgcgc aatgaatggg gttgattatg tattccatgc tgcagcatta 7021 aaacaagttc catcttgtga attttacccg atggaagctg ttaaaactaa cgttaatggg 7081 actgaaaatg tattaaatgc agctattgat tgtggcgtaa aacgtgttgt tttacttagt 7141 acagataaag ccgtatatcc tataaatgcg atgggaatat caaaagcgat ggcggaaaag 7201 gtgctaattg caaaagctag acggcaggat aacgacaaaa caatcatgtg tgcaacccgt 7261 tatgggaatg ttatggcatc ccgtggctca gtaataccat tgttttttga aaaaataaaa 7321 ctcaataaac cattgactgt aactgatcct aatatgacaa ggttcctcat gtcccttgaa 7381 gattctgttg atcttgttct tcatgcattt aaaaatggaa gacaaggcga tatttttgtt 7441 caaaaggcac ctgcttgtac cattggggac ttaacagagg ccataaaaca gttatgtgac 7501 agtaacatag aaactaaaat aatagggaca cgtcacggtg aaaaacttta tgagtcgctg 7561 gtttctcgag aagaaatggt aaaagctgaa gatatgggac gatattatcg cattccagcg 7621 gataatcgag acttaaatta tgataaatat attattaaag gtcagataga tatcaataaa 7681 cttgatgact atacttcaca taacactgaa cggttgaatg ttgaacaaat taaggcgttg 7741 cttttgacat tgaaatatgt tcaaaaggaa cttggcattt aaataaattg ttatgttgca 7801 aatccaataa taatattgga tttgcttatc tgagtggttt atatgatcaa acttagtatc 7861 ttaataccta cgaaaaatcg tagtgagtac ttggatgatg ttttatcgag tttattgact 7921 tggaaacgag atgattttga agttattatt caagataata gcgatgattt gaaaacacat 7981 aatgttgtag aaaaatacag atatgatcat cgcgttaagt atttttatgt taaagaacat 8041 ctatctgtga ttgataattt tgagtatgcc gtaaaaaata gctcaggaaa gatcattaca 8101 gcaattggtg atgatgatgg aatattacct attgtaatgg atgttgcgga ttggatgctg 8161 aaaaattcag tagaagcggt tttaactaat aaagcggagt acgtgtggcc tgatcttata 8221 agcaagtaca actcgaatta taataatagt aaactcaaat ttaataaaaa ttattcttat 8281 gattatraaa aaattaattg tatcgatgag ttcttgcagg tttgtcgcca cggaggtacc 8341 agtatgggaa ggttaccacg catatattat ggccttgtct gtagggaatc ccttgaccat 8401 gtatttaatc taacaggaca atatttcccc ggaccaagcc ctgatatggc taatgcagca 8461 agtttgagtt tagttataaa gagtttctat tatttaaatt ttccagtttt tattgctggt 8521 tcatcctcaa aaagtacagc cggtttgggg ttaaaacgta agcatcttgg tgctatatcc 8581 gaaattaaac acctacctat gaatacaatg gctgagtgga ataaaaatat acccgatttt 8641 tggtctggtc ctactatatg ggcacaaagt atttcaagtg taataaaaaa aatggagcca 8701 acttatgatt taaattatga atatctatat gcccgatgtt ttgtattttt tcctcagaaa 8761 agcaaatgta tttatttagc attaagacaa tatggtatat caggaaaaac tgttatcaaa 8821 ataatatatt ggtatgtata taattggaaa ttaagatttg ttcatttgtt acctaagctt 8881 ttagctaaga tagggaaaaa agatcataac attatcacta ttgatgaaca attaaaaata 8941 tcacaggcag ttgactcgtt actttctttt actcaaaaaa gtgatttaag caagtgagtg 9001 aaaatatatt atccagattg acattaaata gtgcccagaa atgtctactg acaggattaa 9061 ttattatact tttggggccg tatggtgggg cgttgggatt tgctctggtt gtctgtgggg 9121 caggattaga aaaaggtcga attgaaagat atattttatg tgttttttct ttcttcatat 9181 ttacattatg ctcagtacag ggcgtatttc actcttctaa ttttaatact atatctttat 9241 tcaccgcatt atgtacggtg atggttctat tcttggtgga taacaattca ttaatattga 9301 ttctggataa gttaatattc gtaatatctg taactgttcc aatagctctg attattgcca 9361 tttcacagac tttaggtgta tatccttttg aatttaggag taatgaagtt cttggagtaa 9421 tgcgatttac atttttattc gccgaaccat ctcattattc tatacttttg tctttatgct 9481 ctttaatttc tttggtgaaa aagacaaatt tatttacaag gtttattctt ttatttggac 9541 ttttgatgac atggtcacta tctggtttct gtatttttac ttttggttat attgcttata 9601 agttttatac aagaggcata ataaaatatt ttatcatttc actgcttctc ggatctataa 9661 ttactttttt atggtttttt aaattacaat atagtgattt ctggttggtt gctaaaattg 9721 attcaataat tcaattgttc tcaggtaaca cagttggtgc aagcagtgca cttcttagat 9781 ttaactcgat tattattggc ccatatttca ttaatgagaa aattaactca gataattttc 9841 ttttatcctt ttttggatta ggttttggaa acttatctga ctttgtctcg gtatattatc 9901 aatctaattt tggattgcca gacgtctatg atgctaataa tattatttca aatgttataa 9961 tatcaagtgg tatagttgga ttggttttct atatcctttc ctttagctac ttcactggat 10021 taaacttagt taatatattg ttcgcagcta taatattact atgtttgtct ttgtttagtg 10081 gctatgcatt cggaccattt gcgattctta ctttactttt atcaagagct tttattttat 10141 tggttataaa tcatgacaaa gcacgcatca atcgatgtac ttatttcaac acatggttcg 10201 agaataaaac aggttcctaa tatattacat gattttaaaa gaaatgttcg ctatataatc 10261 tgtcaccaga attttgaact atatgaacca aacgagattt tgttaaatag aactgatgta 10321 atatatatta ggtcaacaag taatggggtg actaaaagta gaaatattct tttagataaa 10381 gcagatgcag atttaattta tttttgtgat gatgatgttg tattaagtga aaattttgat 10441 gagattatta taaatagtca taaaaaatat tctgatgatg taattctttt taacattcgg 10501 gatgaaaatg gtgaacttcg taaaaaatat ccaaatgaag tagtaaaaaa aacgcgattt 10561 aatattttat ctgttggtac aattgaaata tcgctaaaga acagcccgtt attacctaaa 10621 tttccggagg atatgggggc agggacatca ttacctgtgg gggatgaggc cgtttttttg 10681 tctaccttta tcaaaaaagg gaagagtata cgttatatac ctaatacaat agctattcat 10741 ccaagagaat caaccggtct agaggtaact aatggttcta tctactctag aggtgttgct 10801 cttaatcgta tttatggtat ctatgcattt cttgtggcta ttttctttct gttgagaaga 10861 aggagccttt taaaaactga tagaggagtt ttgcaggggg gtattttatt ttttaaaggc 10921 gtcttactcg gtaagtaaaa aggaatagtt gtgaaaatat ctattgtaac agcaacatat 10981 aattcatggc catatattaa ggaaacttat gattcaatcg caaatcaatc ttataaaaat 11041 tgggaatggc atgtaacaga cgattgttcg tctgattata cctatcaata tctaaaacaa 11101 ttagctgata gagattgtag aattgtaatt catcgaaatg agattaatag tggagcggca 11161 gttgcaagaa ataaatcgtt atcgcaatgc tctggagatt atatcgcttt tatagatagc 11221 gatgaccttt ggaacccaga gaaactcgaa agccaattga aatttatggt tgataaccaa 11281 ttagattttt catttacggg atatgaggtt attgatgaat ctggcaagtc aataaaaaaa 11341 tatattgaca ttaatcaaac acaagctatt agctacgaag atatgctaaa gaaaaaagca 11401 acgttgggtt gctcaactgt tatgcttcgt aggctggctt tctccgagct ttccatgcct 11461 gatcttcgca ctggtcagga ttatgcatta tggttaaaat tattaaaaac tggaattaga 11521 gcagttcctt ataataaatc tttgatgaaa tatcgtatag tttcctcctc tatttcacga 11581 aataaatatc gtaaagcaaa acgacaatgg caaatatacc gcgagctaga aaaactttct 11641 ttggttaagt ctatctattg ttttttattt tatgcttgga gagctatttt taggaaataa 11701 gtcaatttta tttgccattt ttctaaaagg aattaaaata ttgagaagtg taaataataa 11761 cgagtagtca tcaataaata atagagttat taacttaccg catatcatgt ggtaccccct 11821 gacaggagta aacaatgtca aagcaacaga tcggcgtcgt cggtatggca gtgatggggc 11881 gcaaccttgc gctcaacatc gaaagccgtg gttataccgt ctctattttc aaccgttccc 11941 gtgagaaaac ggaagaagtg attgccgaaa atccgggcaa gaaactggtt ccttactata 12001 cggtgaaaga gtttgttgaa tctctggaaa cgcctcgtcg catcctgtta atggtgaaag 12061 caggtgcagg cacggatgct gctattgatt ccctcaagcc atacctcgat aaaggtgaca 12121 tcatcattga tggtggtaat accttcttcc aggacaccat tcgtcgtaac cgtgagcttt 12181 ctgccgaagg ctttaatttt atcggtaccg gtgtctccgg tggtgaagaa ggcgcgctga 12241 aaggtccttc cattatgcct ggtgggcaga aagaagccta tgaactggtt gctccgatcc 12301 tgaccaaaat cgccgcagtg gctgaagacg gcgagccatg cgttacctat attggtgccg 12361 atggtgcagg tcactatgtg aagatggttc acaacggtat tgaatacggc gatatgcagc 12421 tgattgctga agcctattct ctgcttaaag gtggcctgaa tctttccaac gaagaactgg 12481 cgcagacgtt taccgagtgg aataacggtg aactgagcag ctacctgatc gacatcacca 12541 aagacatctt caccaaaaaa gatgaagacg gtaactacct ggttgatgtg attctggatg 12601 aagcagcaaa caaaggtacc ggtaaatgga ccagccagag cgcgctggat ctcggcgaac 12661 cgctgtcgct gattaccgag tctgtgtttg cacgttatat ctcttctctg aaagatcagc 12721 gtgttgccgc atctaaagtt ctctctggcc cacaagcgca gccagctggc gacaaggctg 12781 agttcatcga aaaagttcgc cgtgcactgt atctgggcaa aatcgtttct tacgctcagg 12841 gcttctctca gctacgcgcc gcgtctgaag agtacaactg ggatctgaac tacggcgaaa 12901 tcgcgaagat tttccgtgct ggctgcatca tccgtgcgca gttcctgcag aaaatcaccg 12961 atgcctatgc cgaaaatccg cagatcgcta acctgctgct ggctccgtac ttcaagcaaa 13021 ttgccgatga ctaccagcag gcgctgcgtg atgtcgttgc ttatgcggta cagaacggta 13081 tcccggttcc gaccttctct gcggcagttg cctattatga cagctaccgc gccgctgttc 13141 tgcctgcgaa cctgatccag gcacagcgtg actatttcgg tgcgcatact tataagcgca 13201 ttgataaaga aggtgtgttc cataccgaat ggctggatta atctgattta aatcaattaa 13261 tcaatgcaag gcccggagaa accctccggg cttttttatt atacaaagcg gcaggttatg 13321 gccttttttt ataatttata gttaaaaacg cgatataata cagcgccgca cagcaggatc 13381 gctgccttga cagttcatca acatcagcgt taaaaatccc gcagtagatg aagttgtggt 13441 ggtggattaa tgaccactct aaatgtttaa ccggaagaag tcagagctaa tgaaaataac 13501 aatttcagga acaggttatg ttggtctttc aaatggtatt ctgattgcgc aaaatcacga 13561 agtggttgca ctggatatcg ttcaggccaa agtggacatg cttaacaaga agcagtcacc 13621 gattgttgat aaggagattg aagagtatct ggcgactaaa gatctcaatt tccgcgctac 13681 gacagataag tatgacgcgt ataaaaatgc cgattacgtt attattgcca cacctaccga 13741 ttatgatccg aaaacgaatt attttaatac ctcaagcgtg gaagcggtca ttcgcgatgt 13801 gacagaaatt aatcctaatg ctgtcatggt gattaaatct acaattccgg taggtttcac 13861 caaatcaatt aaagaacgtc tgggtatcga taaccttatt ttctctccgg aattcctccg 13921 cgaaggtaaa gctctttacg ataaccttca tccgtcacgt attgtcatcg gtgagcgttc 13981 agaacgcgca gaatgttttg cggcgttatt acaggaaggc gcgattaagc aaaatatccc 14041 tacactgttt accgactcca ctgaagcaga agcgattaaa ctgtttgcta atacctatct 14101 ggcgatgcgc gtagcatact ttaatgaact ggatagctat gcagaaagtt tagggctgaa 14161 tactcgccag attatcgaag gcgtttgtct cgatccgcgt attggcaacc attacaacaa 14221 cccgtcgttt ggctatggtg gttattgtct gccgaaagat accaagcagt tactggcgaa 14281 ctaccagtct gtgccgaata acctgatttc ggcaattgtc gatgcaaacc gcacgcgtaa 14341 agactttatt gccgatgcca ttttgtcacg caaaccgcaa gtggtgggta tttatcgtct 14401 gattatgaag agcgggtcag ataacttccg cgcgtcttcc attcagggga ttatgaaacg 14461 tattaaggcg aaaggcgttg aagtgatcat atacgagcca gtgatgaaag aagactcatt 14521 cttcaactct cgcctggaac gagaactcgc caccttcaaa caacaagccg acgtcattat 14581 ttccaaccgt atggcagaag agcttaaaga tgtggcagat aaggtctata cccgagatct 14641 ctttggcagc gactaacatc ctgtgatcag ggcgattttc gccctgatta tcttttgtta 14701 cttacggaac aattcattat ttttatcact tatcctatag cattcacgag gattatcgct 14761 aaactatggc gacttaaaat tatttcgtcc gttagggtaa tgatgagagt agaaaataat 14821 aatgtttctg ggcaaaacca tgacccggaa cagattgatt tgattgattt actggtgcag 14881 ttgtggcgcg gcaagatgac aattatcatc tctgtcattg ttgctattgc cctggctata 14941 gggtatctgg ccgttgccaa agaaaagtgg acttccacag ctatcgttac tcaacctgat 15001 gtggggcaaa tcgctggtta taccaacgcg atgaatgtga tttacggtcc ggctgtaccg 15061 aaagtctccg acattcaggc ttcacttatc ggacgttaca gcactgcatt ttcagcatta 15121 gcggaaacgc tggataacca ggaagaagca gagaagctca ccattgagcc taccgtcaaa 15181 aatcagtcat tacctttagc ggtgtcttat gttggtgaaa cacctgaagg tgcgcagaaa 15241 cagctggcga agtatatcca gcaagttgac gaccaggtaa atgaagaact ggaaaaagac 15301 ctgaaagaca acatcgcgct gcgtatgaaa aacttgcagg attctctgaa aactcaggaa 15361 gtagttgcgc aggagcagaa agatctgcgt atccgtcaga ttcaggaagc gttgcagtat 15421 gcgaatcagg cgcaggtgac aaagccgcag attcaacaga cgcaggacgt gacgcaagat 15481 acgatgttcc tgctgggaag tgacgcgctg gagtcgatga ttaagcatga agcgacccgc 15541 ccactagttt tctcgccaaa ctactatcag actcgtcaaa acctgctgga tatcgaaagt 15601 ttaaaggttg atgatcttga tatccatgct taccgctatg tgatgaaacc gacgttacct 15661 attcgtcgcg atagcccgaa aaaggcaatt accttgattc tggcggtact gctgggcggc 15721 atggttggcg cggggattgt gctggggcgt aatgctctac gcaattacaa cgcgaaataa 15781 tctttgcagc tttaaagaaa acgggcaggg tggtgacacc ttgcccgttt ttttttgccg 15841 gatgcgacaa caatatcgca tccgcttacc ccgcaactca ctgatgccgt ttacgcaggt 15901 tctcaatcac cgtcgttaaa tccagatcct gatcctgcaa caataccaac aggtgataca 15961 tcaaatcaga cgcctcgttg gtcagctcaa agcggtcatg tacagtcgcg gccagtgcgg 16021 tttccacgcc ttcttcgccc actttctgcg caatgcgttt ggtgccgctg gcatacagtt 16081 tggcggtgta ggaggtttcc gggtcggcag atttacgttc ggcgagcagt tgttccagtt 16141 gatacaggaa cagccactgg tgagcggtgt cgccgaagca gctgctggtg cctttgtggc 16201 aggttggccc gattggattt gccagcacca gtaacgtgtc gttgtcgcag tccggggcaa 16261 tactcaccac attgaggaaa ttgcccgacg tttcgccttt ggtccacagt cgctgtttag 16321 tgcgcgagaa gaaggtgact ttgccgcttt cgatggtttt gtctaaggct tccgggttca 16381 tatagcccag cattaacact tcgccggata ccgcgtgttg cacaatcacc ggcatcagtc 16441 cgtcggtttt ttcccagtcc agttcgcgac gttgttgttc tgttaacata tcctgatctc 16501 cacgccctgt gttgccaggt acgcttttaa ttcaccaata ttgattattt gtttgtggaa 16561 tacggaagct gccagcgcgc cgtcaacgtc ggcatcacgg aaggcttcga ggaagtgttc 16621 catggtgccc gcgccaccgg aggcaattag cgggacgtgg caaacttcac gtactttttt 16681 cagttgttcg aggtcgtaac cgttacgcac gccgtcctga ttcatcatgt tgaggacgat 16741 ttctccggca ccgcgttttt gcacttcctg tacccagtcg agcgtttccc actgagtgac 16801 gcgggtgcgg //