LOCUS AB728565 4208 bp DNA linear BCT 01-MAR-2013 DEFINITION Gluconobacter japonicus fdhS, fdhC, fdhL genes for small subunit of fructose dehydrogenase, cytochrome subunit of fructose dehydrogenase, large subunit of fructose dehydrogenase, complete cds, strain: NBRC 3260. ACCESSION AB728565 AB378084-AB378086 VERSION AB728565.1 KEYWORDS . SOURCE Gluconobacter japonicus ORGANISM Gluconobacter japonicus Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae; Gluconobacter. REFERENCE 1 (bases 1 to 4208) AUTHORS Kawai,S., Goda-Tsutsumi,M, Yakushi,T., Kano,K. and Matsushita,K. TITLE Direct Submission JOURNAL Submitted (12-JUN-2012) to the DDBJ/EMBL/GenBank databases. Contact:Toshiharu Yakushi Yamaguchi University, Faculty of Agriculture; Yoshida 1677-1, Yamaguchi, Yamaguchi 753-8515, Japan URL :http://web.cc.yamaguchi-u.ac.jp/~oubi/ REFERENCE 2 AUTHORS Kawai,S., Goda-Tsutsumi,M., Yakushi,T., Kano,K. and Matsushita,K. TITLE Heterologous Overexpression and Characterization of a Flavoprotein-Cytochrome c Complex Fructose Dehydrogenase of Gluconobacter japonicus NBRC3260 JOURNAL Appl. Environ. Microbiol. 79, 1654-1660 (2013) REMARK DOI:10.1128/AEM.03152-12 REFERENCE 3 AUTHORS Ameyama,M., Shinagawa,E., Matsushita,K. and Adachi,O. TITLE D-fructose dehydrogenase of Gluconobacter industrius: purification, characterization, and application to enzymatic microdetermination of D-fructose. JOURNAL J. Bacteriol. 145, 814-823 (1981) COMMENT FEATURES Location/Qualifiers source 1..4208 /culture_collection="NBRC:3260" /db_xref="taxon:376620" /mol_type="genomic DNA" /organism="Gluconobacter japonicus" /strain="NBRC 3260" CDS 93..644 /codon_start=1 /EC_number="1.1.99.11" /experiment="heterologous expression of genes for flavoprotein cytochrome c complex fructose dehydrogenase" /gene="fdhS" /product="small subunit of fructose dehydrogenase" /protein_id="BAM93250.1" /transl_table=11 /translation="MEKIADSGPVQIFLSRRKLLAFSGASLTVAAIGAPSKGSTQDVV ASNRDSISDFMQLSAFATGHKNLDLNIGSALLLAFEAQKHDFSTQIKALREHITKNNY QDVEALDAAMKDDPLHPTLIQIIRAWYSGVIEDETNAKVYAFEKALMYQPSRDVVVIP TYAHNGPNYWVSEPASVDVMPAF" CDS 663..2123 /codon_start=1 /EC_number="1.1.99.11" /experiment="heterologous expression of genes for flavoprotein cytochrome c complex fructose dehydrogenase" /gene="fdhC" /product="cytochrome subunit of fructose dehydrogenase" /protein_id="BAM93251.1" /transl_table=11 /translation="MRYFRPLSATAMTTVLLLAGTNVRAQPTEPTPASAHRPSISRGH YLAIAADCAACHTNGRDGQFLAGGYAISSPMGNIYSTNITPSKTHGIGNYTLEQFSKA LRHGIRADGAQLYPAMPYDAYNRLTDEDVKSLYAYIMTEVKPVDAPSPKTQLPFPFSI RASLGIWKIAARIEGKPYVFDHTHNDDWNRGRYLVDELAHCGECHTPRNFLLAPNQSA YLAGADIGSWRAPNITNAPQSGIGSWSDQDLFQYLKTGKTAHARAAGPMAEAIEHSLQ YLPDADISAIVTYLRSVPAKAESGQTVANFEHAGRPSSYSVANANSRRSNSTLTKTTD GAALYEAVCASCHQSDGKGSKDGYYPSLVGNTTTGQLNPNDLIASILYGVDRTTDNHE ILMPAFGPDSLVQPLTDEQIATIADYVLSHFGNAQATVSADAVKQVRAGGKQVPLAKL ASPGVMLLLGTGGILGAILVVAGLWWLISRRKKRSA" CDS 2145..3779 /codon_start=1 /EC_number="1.1.99.11" /experiment="heterologous expression of genes for flavoprotein cytochrome c complex fructose dehydrogenase" /gene="fdhL" /product="large subunit of fructose dehydrogenase" /protein_id="BAM93252.1" /transl_table=11 /translation="MSNETLSADVVIIGAGICGSLLAHKLVRNGLSVLLLDAGPRRDR SQIVENWRNMPPDNKSQYDYATPYPSVPWAPHTNYFPDNNYLIVKGPDRTAYKQGIIK GVGGTTWHWAASSWRYLPNDFKLHSTYGVGRDYAMSYDELEPYYYEAECEMGVMGPNG EEITPSAPRQNPWPMTSMPYGYGDRTFTEIVSKLGFSNTPVPQARNSRPYDGRPQCCG NNNCMPICPIGAMYNGVYAAIKAEKLGAKIIPNAVVYAMETDAKNRITAISFYDPDKQ SHRVVAKTFVIAANGIETPKLLLLAANDRNPHGIANSSDLVGRNMMDHPGIGMSFQSA EPIWAGGGSVQMSSITNFRDGDFRSEYAATQIGYNNTAQNSRAGMKALSMGLVGKKLD EEIRRRTAHGVDIYANHEVLPDPNNRLVLSKDYKDALGIPHPEVTYDVGEYVRKSAAI SRQRLMDIAKAMGGTEIEMTPYFTPNNHITGGTIMGHDPRDSVVDKWLRTHDHSNLFL ATGATMAASGTVNSTLTMAALSLRAADAILNDLKQG" BASE COUNT 1064 a 1166 c 1011 g 967 t ORIGIN 1 attccaaaca aaaaatagtc atttctgtta ttcgtgaaaa ttttgaatga agaggcacac 61 gcatatcaaa ttatgtcttc aggagaaggc cattggaaaa aatagctgat tccggccctg 121 ttcaaatctt tctttcgcgt agaaagcttc tggctttttc cggtgccagc ctaacagtcg 181 cagcaattgg agcaccatcg aaaggatcta ctcaggacgt tgtggcatca aatcgcgata 241 gcatttcgga ttttatgcag ctttccgctt ttgccaccgg tcacaaaaat ctggatctca 301 atatcggatc agcacttctg ttggcatttg aagctcagaa gcatgatttt tctactcaaa 361 taaaggctct tcgcgaacat attactaaaa ataattatca ggatgtcgaa gcactggatg 421 cagccatgaa agatgacccc ctgcatccca cgctcatcca gattatccgg gcgtggtatt 481 caggtgtcat cgaagatgaa acaaacgcta aagtttacgc tttcgaaaaa gcactcatgt 541 atcagccgtc ccgcgatgtc gtggtcattc cgacatatgc tcataacggg cccaattact 601 gggtctcgga acccgcatcc gtcgatgtca tgccggcatt ttaaggactt tattccgtga 661 ttatgcgata ttttcgccct ctgtccgcca cagccatgac aaccgttctg cttctcgcag 721 ggacgaacgt acgggcgcaa ccgacagaac caacacctgc ttcagcgcat cgcccctcca 781 tcagccgcgg tcattatctg gcaattgccg ccgattgtgc ggcctgccat accaatgggc 841 gtgacggtca atttcttgct ggtggttatg ccatttcttc cccaatgggg aatatctatt 901 caaccaatat tacgccgtcg aagacgcacg gtatcggaaa ctatacactg gagcagtttt 961 ctaaggctct ccggcacggt attcgcgccg atggcgcgca actgtatccc gccatgcctt 1021 atgacgctta caatcgtctg acggatgaag acgtcaaatc gctctacgct tacatcatga 1081 ctgaagtaaa acccgtagat gcaccttctc ccaagacgca actccccttc ccgttttcaa 1141 tccgtgcatc actcggtatt tggaaaattg cggcaagaat cgaaggcaaa ccctatgtct 1201 ttgatcatac ccacaatgac gactggaatc gtggtcgcta cctggtcgat gaactcgccc 1261 attgtggaga gtgtcacaca ccgcgtaact ttcttctagc gcccaatcag tccgcttatc 1321 ttgctggtgc ggacatagga tcatggcgtg caccaaatat taccaatgcc cctcaaagcg 1381 gtattggcag ttggtcggat caggatctct tccaatatct gaaaactgga aaaaccgcac 1441 atgctcgtgc agcaggacca atggcagaag caatcgagca tagcctacaa tatcttccgg 1501 atgccgatat ttctgccata gttacatatc ttcgttcggt tccggcaaag gcagaaagcg 1561 gtcaaacagt cgctaatttt gagcatgccg gccgcccatc cagttacagt gttgctaacg 1621 ctaactcccg tcgcagcaat agtactctga caaaaacaac cgatggtgct gcactctacg 1681 aggctgtgtg cgccagttgc catcaatctg acggtaaagg gtccaaagac ggttattatc 1741 cttccctcgt aggaaatacg acgaccgggc aactcaatcc aaatgatctg atcgccagta 1801 ttctttacgg tgtggaccgc acaacggata accacgaaat cctgatgccg gcttttggtc 1861 cagactctct cgtacagccc ctgacagatg agcagatcgc cacaatcgcg gactacgttc 1921 taagccactt tggcaatgcc caggccacag tctcggcaga tgcggtcaaa caggtgcggg 1981 cgggcggaaa gcaggttcct ctcgccaagc tcgccagtcc gggcgtgatg ctgcttctcg 2041 gaactggtgg catcctggga gccattctgg tggtagccgg cctctggtgg ctgatcagcc 2101 gtcgcaaaaa gcgttccgct taataagatt caagggtcaa aatcatgtct aatgaaacgc 2161 tttcggctga tgtcgtcatc attggagcag gaatctgcgg ctccctgctc gcccacaaac 2221 tcgtccgcaa cggactttct gtgctgctcc tggatgcagg tccccgccgg gaccgctccc 2281 agatcgtaga aaactggcgg aacatgccgc ctgacaacaa gtcccagtac gattatgcaa 2341 caccttaccc cagcgtaccc tgggccccgc ataccaacta cttcccagac aataactacc 2401 tgattgtcaa aggcccggac cggacggctt acaagcaagg aatcatcaaa ggcgtcgggg 2461 gtacaacctg gcactgggcg gcttcaagct ggcggtacct gcccaacgat ttcaagctgc 2521 attccacata tggcgtgggc cgcgattacg ccatgagcta cgacgaactt gagccttact 2581 attacgaggc cgagtgcgaa atgggcgtga tgggaccaaa cggcgaagag attacaccgt 2641 ctgcgcctcg ccaaaatcca tggccgatga cctccatgcc ttacggatat ggagaccgca 2701 ctttcacgga gatcgtcagc aagctcggtt tctcaaacac tcctgttccg caggcacgga 2761 acagtcgtcc ttatgatggc cgaccacaat gctgtggcaa caacaactgc atgccaatct 2821 gcccgattgg cgcgatgtac aatggcgtat acgcggcaat aaaagcggaa aagctgggcg 2881 caaaaattat tcccaatgct gtcgtctatg cgatggaaac ggatgcaaaa aaccggatca 2941 cagcgattag cttctatgat cccgacaagc agtcccatcg cgtcgttgca aaaacatttg 3001 tgatcgccgc aaacgggatc gaaacaccga aactgctgct tctggcggca aatgatcgaa 3061 accctcatgg gattgccaac tcatcagacc ttgttggccg gaacatgatg gaccatccgg 3121 gcatcggcat gagcttccag tctgcggagc ccatctgggc tggtggaggc tcagtccaga 3181 tgagttccat caccaacttc cgtgatggcg atttccgctc ggagtatgct gccacgcaga 3241 tcggctacaa caatacggcc cagaactcac gcgccggcat gaaagctctg tccatggggt 3301 tggttggcaa gaagctggat gaagaaatcc gccgtcgtac ggcgcatggt gtggacattt 3361 atgccaacca tgaagtcctc ccggacccca acaaccgtct tgttctctcc aaagactata 3421 aggatgcgct cggtattcca catcctgaag tcacctacga tgttggggag tatgttcgga 3481 agtcagctgc catctcaaga cagcgcctga tggatatcgc caaagccatg ggcggtacgg 3541 aaatcgagat gactccgtat tttacgccca acaaccacat caccggtggc actatcatgg 3601 gccatgatcc acgggattca gtggtcgata aatggctccg gacccatgat cattccaatc 3661 tgttccttgc aacaggcgcc accatggcag cgtccggtac ggtcaattca acgttaacaa 3721 tggccgcact gtcattacgc gcggcagatg ccattctcaa tgacctgaaa caggggtaag 3781 tcttttgaac acagtgtttc aacgccacgc tattggcgct tttctaagta ttttcatcgc 3841 atcagcaagt ctgactggtc tcgcatcagc aaaggatttg accgtgaatc tggaaacatc 3901 caatactctc attgaccaag ccaagaaact ggcgactgcc agtcataacc gcgtcgccat 3961 cgctattgtc gatgcagggg gaaatcttgt atcttttcaa aaaatggatg gcacccaact 4021 tgggagcatt gagctggcga tccgtaaggc caagactgct ctttccttcg cccgcccgac 4081 tgctgacatg gaacatgccc tcaatagtgg gaattacatg atcagcacgc tccccaatgc 4141 tctgcccgca ggtggaggat acccgatcat ggtgaacaat gaacttgtgg tcggccatca 4201 taaggacg //