LOCUS       AB517737                1602 bp    RNA     linear   VRL 07-JAN-2011
DEFINITION  Human picobirnavirus RdRp gene for RNA dependent RNA polymerase,
            partial cds, isolate: GPBV11.
ACCESSION   AB517737
VERSION     AB517737.1
KEYWORDS    .
SOURCE      Human picobirnavirus
  ORGANISM  Human picobirnavirus
            Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes;
            Durnavirales; Picobirnaviridae; Orthopicobirnavirus;
            Orthopicobirnavirus hominis.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Ganesh,B., Nagashima,S., Kobayashi,N. and Krishnan,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2009) to the DDBJ/EMBL/GenBank databases.
            Contact:Triveni Krishnan
            National Institute of Cholera and Enteric Diseases, Division of
            Virology; P 33 CIT Road, Scheme XM, Beliaghata, Kolkata, West
            Bengal 700010, India
REFERENCE   2  
  AUTHORS   Ganesh,B., Nagashima,S., Kobayashi,N. and Krishnan,T.
  TITLE     Molecular characterization of segment 2 of genogroup 1
            picobirnavirus from Kolkata, India
  JOURNAL   Published Only in Database(2011)
COMMENT     
FEATURES             Location/Qualifiers
     source          1..1602
                     /collection_date="2007-10-03"
                     /db_xref="taxon:145856"
                     /geo_loc_name="India:Kolkata"
                     /isolate="GPBV11"
                     /isolation_source="Faecal specimen of diarrhoea case"
                     /mol_type="genomic RNA"
                     /note="First PCR Primer C or Primer TRLase Comp 42-1
                     5-GGTCTAGAGGTGATCAGTTAACTAGTTAGTACTC-3"
                     /note="Reverse Transcription Primer B or Comp42 primer
                     5-AAAGGTCTAGAGGTGATCAGTTAACTAGTTAGTACTCGAGGG-3"
                     /note="Second PCR Primer D or Primer T4 RLase Comp 42-2
                     5-TCAGTTAACTAGTTAGTACTCGAGGG-3"
                     /note="T4 RNA Ligase reaction Primer A
                     5-CCCTCGAGTACTAACTAGTTAACTGATCACCTCTAGACCTTT-3 whose 5
                     prime end is phosphorylated and whose 3 prime end
                     incorporates an NH2 blocking group"
                     /note="The Single primer amplification protocol was
                     carried out using four primers as shown below"
                     /organism="Human picobirnavirus"
     CDS             <1..>1602
                     /codon_start=1
                     /gene="RdRp"
                     /product="RNA dependent RNA polymerase"
                     /protein_id="BAJ53293.1"
                     /transl_table=1
                     /translation="GGFRMKKSKPSISEYFELPNPGLWAYFKHTVSGNTEDYRPPFWK
                     GQTREAVLAKWQKVFDGTNVKRTLPGLYDFEMDMKSKVGPMSIQLPLKDRLESIENYF
                     TMVQLDSEPIEAKAIDSACDFFAKAGGIRFRSKQRTAENMRLSTNSGPPWFTKRRKVL
                     DEALNSNIYFDQARNEYMVSTPKGTYKLAAVLGWRGQEGGISLDDVKQRVIWMMSMAL
                     NIEELQFYQPAIEAIQKNGLIPAYASMDAVDDEVTALFATKGADDVVICTDFTKFDQH
                     FNSNMQDAARIIENSLMNSNTSWDDRVFYAKFRIPIICSEDLMYTGAHGMGSGSGGTN
                     FDECMSHKGLQFEAAIRKKQKLNPHSMAYGDDGILTFPGIDVKDVIDTYSRHGQEMNA
                     SKQMVSKHECVVLRRWHSTKYTIDNRMVGVYSTFRALGRLLAQERYYDPDKWSGEMVT
                     LRALSIIENCKWSPVFHEFIDFVMTGDKFRLGLNLPGFFDNLEAKAKEAIDEFEDFLG
                     YTKNLQNKDAASGISNWEVVKYLKSK"
BASE COUNT          482 a          296 c          387 g          437 t
ORIGIN      
        1 ggaggttttc gaatgaagaa atcgaaacca agtatctcgg agtactttga acttccgaac
       61 ccaggcttgt gggcgtattt caagcatacg gtatcaggta ataccgaaga ttatcgtccc
      121 ccgttttgga aggggcagac aagagaagct gttttggcta aatggcagaa agtgtttgac
      181 ggtaccaatg tcaaacggac acttcctggt ctttatgact ttgagatgga tatgaagagt
      241 aaagttggac ccatgtctat tcaattacca cttaaagatc ggttggagtc tattgaaaat
      301 tacttcacta tggtgcagct cgatagtgag cctattgaag cgaaagccat tgattcggca
      361 tgtgactttt tcgcgaaagc aggtggtatt cgtttccgca gtaaacagcg gacagcagag
      421 aacatgaggc tctcaacaaa ctcaggtcct ccgtggttta ccaaaagaag aaaagtgtta
      481 gacgaagcac ttaattcaaa catctatttc gatcaagctc gtaatgagta tatggtatcg
      541 actcctaaag gtacctataa gctagccgcc gtgttgggct ggcgtggtca agagggtggt
      601 atctctctag atgatgttaa acaacgcgtt atatggatga tgagtatggc actcaacatc
      661 gaagagctac aattttatca gccagcgatc gaagcaatcc agaaaaatgg acttatcccc
      721 gcatatgcgt ctatggacgc tgttgatgac gaagtaacgg cattgtttgc aactaaaggt
      781 gctgatgacg tggttatatg tacggacttc accaaatttg atcaacattt caactcaaat
      841 atgcaagatg cagccagaat cattgagaac tccctcatga attcaaatac atcttgggat
      901 gatcgcgtgt tttatgcgaa atttcgtata cctattatct gtagtgagga tcttatgtat
      961 accggggcgc acggaatggg atctggttcc ggcggtacga atttcgacga gtgtatgagc
     1021 cataaaggct tacagttcga ggcagctatc cgtaagaaac aaaaacttaa tccccatagt
     1081 atggcttatg gtgatgatgg aatattaact ttcccgggta tagatgtcaa agatgtaata
     1141 gacacttatt cacgtcatgg gcaggaaatg aatgcatcaa agcagatggt gtctaaacat
     1201 gagtgtgtgg ttttgcgtag atggcacagc accaaatata ccatcgataa ccgcatggtc
     1261 ggtgtctact cgactttccg tgctttagga aggttgctag cacaagaaag gtattatgat
     1321 ccagataagt ggagcggaga aatggtaacg ttgagagcgt tgtcaataat cgagaattgt
     1381 aaatggtcac ccgttttcca cgagtttatt gactttgtca tgacaggaga taagtttaga
     1441 ttgggtttaa acttaccagg tttctttgac aatctcgaag ctaaggccaa agaagctatc
     1501 gacgaatttg aagacttctt aggatacact aagaatctac agaataaaga cgcagccagt
     1561 ggtatctcta actgggaagt tgttaagtat ctgaagtcta ag
//