LOCUS       AB451296                 951 bp    mRNA    linear   HUM 02-SEP-2008
DEFINITION  Homo sapiens GTF2B mRNA for general transcription factor IIB,
            complete cds, clone: FLJ08145AAAN.
ACCESSION   AB451296
VERSION     AB451296.1
KEYWORDS    Gateway cloning system.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Kawamura,Y., Nomura,N. and Goshima,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases.
            Contact:Naoki Goshima
            Biomedicinal Information Research Center (BIRC), National
            Institute of Advanced Industrial Science and Technology (AIST);
            2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan
REFERENCE   2  
  AUTHORS   Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R.,
            Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T.,
            Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C.,
            Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M.,
            Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K.,
            Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A.,
            Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y.,
            Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T.,
            Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H.,
            Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T.,
            Imai,J.-I., Watanabe,S. and Nomura,N.
  TITLE     Human Protein Factory: an infrastructure to convert the human
            transcriptome into the in vitro-expressed human proteome of
            versatile utility
  JOURNAL   Unpublished (2008)
COMMENT     This human Gateway entry clone was constructed by recombining an
            attB-attached open reading frame (ORF) fragment with attP
            sequences of the Gateway donor vector pDONR201. The ORF sequence
            in the entry clone is flanked with attL1 sequence (100 nt) -
            spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) -
            spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the
            translational initiation codon (ATG) and is also flanked with a
            spacer nucleotide (G) - attL2 sequence (99 nt) downstream of the
            ORF. This is an N-type clone which has an intrinsic stop codon at
            the end of ORF. DNA sequences of the entire ORF and flanking
            regions were validated by sequencing. Clone structure of the ORF
            and flanking sequences is as follows:
            5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct
            tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3'
            G acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaacgaa
            caggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence. attL
            sequences are described in lower cases).
            Clone information: http://www.HGPD.jp/
            This clone was produced in the "Functional Analysis of Protein and
            Research Application" project supported by the New Energy and
            Industrial Technology Development Organization (NEDO), Japan
FEATURES             Location/Qualifiers
     source          1..951
                     /clone="FLJ08145AAAN"
                     /db_xref="H-InvDB:HIT000487509"
                     /db_xref="taxon:9606"
                     /mol_type="mRNA"
                     /note="Vector: pDONR201"
                     /organism="Homo sapiens"
     CDS             1..951
                     /codon_start=1
                     /gene="GTF2B"
                     /product="general transcription factor IIB"
                     /protein_id="BAG70110.1"
                     /transl_table=1
                     /translation="MASTSRLDALPRVTCPNHPDAILVEDYRAGDMICPECGLVVGDR
                     VIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQ
                     NRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIA
                     SACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRF
                     CSNLCLPKQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGD
                     IAGVADVTIRQSYRLIYPRAPDLFPTDFKFDTPVDKLPQL"
BASE COUNT          283 a          192 c          223 g          253 t
ORIGIN      
        1 atggcgtcta ccagccgttt ggatgctctt ccaagagtca catgtccaaa ccatccagat
       61 gcgattttag tggaggacta cagagccggt gatatgatct gtcctgaatg tggcttggtt
      121 gtaggtgacc gggttattga tgtgggatct gaatggcgaa ctttcagcaa tgacaaagca
      181 acaaaagatc catctcgagt tggagattct cagaatcctc ttctgagtga tggagatttg
      241 tctaccatga ttggcaaggg cacaggagct gcaagttttg acgaatttgg caattctaag
      301 taccagaatc ggagaacaat gagcagttct gatcgggcaa tgatgaatgc attcaaagaa
      361 atcactacca tggcagacag aatcaatcta cctcgaaata tagttgatcg aacaaataat
      421 ttattcaagc aagtatatga acagaagagc ctgaagggaa gagctaatga tgctatagct
      481 tctgcttgtc tctatattgc ctgtagacaa gaaggggttc ctaggacatt taaagaaata
      541 tgtgccgtct cacgaatttc taagaaagaa attggtcggt gttttaaact tattttgaaa
      601 gcgctagaaa ccagtgtgga tttgattaca actggggact tcatgtccag gttctgttcc
      661 aacctttgtc ttcctaaaca agtacagatg gcagctacac atatagcccg taaagctgtg
      721 gaattggact tggttcctgg gaggagcccc atctctgtgg cagcggcagc tatttacatg
      781 gcctcacagg catcagctga aaagaggacc caaaaagaaa ttggagatat tgctggtgtt
      841 gctgatgtta caatcagaca gtcctataga ctgatctatc ctcgagcccc agatctgttt
      901 cctacagact tcaaatttga caccccagtg gacaaactac cacagctata a
//