LOCUS AB451257 1845 bp mRNA linear HUM 02-SEP-2008 DEFINITION Homo sapiens DDX5 mRNA for ATP-dependent RNA helicase DDX5, complete cds, clone: FLJ08070AAAN. ACCESSION AB451257 VERSION AB451257.1 KEYWORDS Gateway cloning system. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1845) AUTHORS Kawamura,Y., Nomura,N. and Goshima,N. TITLE Direct Submission JOURNAL Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases. Contact:Naoki Goshima Biomedicinal Information Research Center (BIRC), National Institute of Advanced Industrial Science and Technology (AIST); 2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan REFERENCE 2 AUTHORS Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R., Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T., Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C., Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M., Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K., Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A., Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y., Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T., Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H., Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T., Imai,J.-I., Watanabe,S. and Nomura,N. TITLE Human Protein Factory: an infrastructure to convert the human transcriptome into the in vitro-expressed human proteome of versatile utility JOURNAL Unpublished (2008) COMMENT This human Gateway entry clone was constructed by recombining an attB-attached open reading frame (ORF) fragment with attP sequences of the Gateway donor vector pDONR201. The ORF sequence in the entry clone is flanked with attL1 sequence (100 nt) - spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) - spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the translational initiation codon (ATG) and is also flanked with a spacer nucleotide (G) - attL2 sequence (99 nt) downstream of the ORF. This is an N-type clone which has an intrinsic stop codon at the end of ORF. DNA sequences of the entire ORF and flanking regions were validated by sequencing. Clone structure of the ORF and flanking sequences is as follows: 5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3' G acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaacgaa caggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence. attL sequences are described in lower cases). Clone information: http://www.HGPD.jp/ This clone was produced in the "Functional Analysis of Protein and Research Application" project supported by the New Energy and Industrial Technology Development Organization (NEDO), Japan FEATURES Location/Qualifiers source 1..1845 /clone="FLJ08070AAAN" /db_xref="H-InvDB:HIT000487470" /db_xref="taxon:9606" /mol_type="mRNA" /note="Vector: pDONR201" /organism="Homo sapiens" CDS 1..1845 /codon_start=1 /gene="DDX5" /product="ATP-dependent RNA helicase DDX5" /protein_id="BAG70071.1" /transl_table=1 /translation="MSGYSSDRDRGRDRGFGAPRFGGSRAGPLSGKKFGNPGEKLVKK KWNLDELPKFEKNFYQEHPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEANFP ANVMDVIARQNFTEPTAIQAQGWPVALSGLDMVGVAQTGSGKTLSYLLPAIVHINHQP FLERGDGPICLVLAPTRELAQQVQQVAAEYCRACRLKSTCIYGGAPKGPQIRDLERGV EICIATPGRLIDFLECGKTNLRRTTYLVLDEADRMLDMGFEPQIRKIVDQIRPDRQTL MWSATWPKEVRQLAEDFLKDYIHINIGALELSANHNILQIVDVCHDVEKDEKLIRLME EIMSEKENKTIVFVETKRRCDELTRKMRRDGWPAMGIHGDKSQQERDWVLNEFKHGKA PILIATDVASRGLDVEDVKFVINYDYPNSSEDYIHRIGRTARSTKTGTAYTFFTPNNI KQVSDLISVLREANQAINPKLLQLVEDRGSGRSRGRGGMKDDRRDRYSAGKRGGFNTF RDRENYDRGYSSLLKRDFGAKTQNGVYSAANYTNGSFGSNFVSAGIQTSFRTGNPTGT YQNGYDSTQQYGSNVPNMHNGMNQQAYAYPATAAAPMIGYPMPTGYSQ" BASE COUNT 574 a 339 c 465 g 467 t ORIGIN 1 atgtcgggtt attcgagtga ccgagaccgc ggccgggacc gagggtttgg tgcacctcga 61 tttggaggaa gtagggcagg gcccttatct ggaaagaagt ttggaaaccc tggggagaaa 121 ttagttaaaa agaagtggaa tcttgatgag ctgcctaaat ttgagaagaa tttttatcaa 181 gagcaccctg atttggctag gcgcacagca caagaggtgg aaacatacag aagaagcaag 241 gaaattacag ttagaggtca caactgcccg aagccagttc taaattttta tgaagccaat 301 ttccctgcaa atgtcatgga tgttattgca agacagaatt tcactgaacc cactgctatt 361 caagctcagg gatggccagt tgctctaagt ggattggata tggttggagt ggcacagact 421 ggatctggga aaacattgtc ttatttgctt cctgccattg tccacatcaa tcatcagcca 481 ttcctagaga gaggcgatgg gcctatttgt ttggtgctgg caccaactcg ggaactggcc 541 caacaggtgc agcaagtagc tgctgaatat tgtagagcat gtcgcttgaa gtctacttgt 601 atctacggtg gtgctcctaa gggaccacaa atacgtgatt tggagagagg tgtggaaatc 661 tgtattgcaa cacctggaag actgattgac tttttagagt gtggaaaaac caatctgaga 721 agaacaacct accttgtcct tgatgaagca gatagaatgc ttgatatggg ctttgaaccc 781 caaataagga agattgtgga tcaaataaga cctgataggc aaactctaat gtggagtgcg 841 acttggccaa aagaagtaag acagcttgct gaagatttcc tgaaagacta tattcatata 901 aacattggtg cacttgaact gagtgcaaac cacaacattc ttcagattgt ggatgtgtgt 961 catgacgtag aaaaggatga aaaacttatt cgtctaatgg aagagatcat gagtgagaag 1021 gagaataaaa ccattgtttt tgtggaaacc aaaagaagat gtgatgagct taccagaaaa 1081 atgaggagag atgggtggcc tgccatgggt atccatggtg acaagagtca acaagagcgt 1141 gactgggttc taaatgaatt caaacatgga aaagctccta ttctgattgc tacagatgtg 1201 gcctccagag ggctagatgt ggaagatgtg aaatttgtca tcaattatga ctaccctaac 1261 tcctcagagg attatattca tcgaattgga agaactgctc gcagtaccaa aacaggcaca 1321 gcatacactt tctttacacc taataacata aagcaagtga gcgaccttat ctctgtgctt 1381 cgtgaagcta atcaagcaat taatcccaag ttgcttcagt tggtcgaaga cagaggttca 1441 ggtcgttcca ggggtagagg aggcatgaag gatgaccgtc gggacagata ctctgcgggc 1501 aaaaggggtg gatttaatac ctttagagac agggaaaatt atgacagagg ttactctagc 1561 ctgcttaaaa gagattttgg ggcaaaaact cagaatggtg tttacagtgc tgcaaattac 1621 accaatggga gctttggaag taattttgtg tctgctggta tacagaccag ttttaggact 1681 ggtaatccaa cagggactta ccagaatggt tatgatagca ctcagcaata cggaagtaat 1741 gttccaaata tgcacaatgg tatgaaccaa caggcatatg catatcctgc tactgcagct 1801 gcacctatga ttggttatcc aatgccaaca ggatattccc aataa //