LOCUS       AB439728                1710 bp    DNA     linear   BCT 12-SEP-2020
DEFINITION  Microbulbifer thermotolerans cgiA gene for iota-carrageenase,
            complete cds.
ACCESSION   AB439728
VERSION     AB439728.1
KEYWORDS    .
SOURCE      Microbulbifer thermotolerans
  ORGANISM  Microbulbifer thermotolerans
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Cellvibrionales; Microbulbiferaceae; Microbulbifer.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Hatada,Y. and Ohta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUN-2008)
            Contact:Yukari Ohta
            Gunma university, Gunma University Center for Food Science and
            Wellness; 4-2 Aramaki, Maebashi, Gunma 371-8510, Japan
REFERENCE   2  
  AUTHORS   Hatada,Y., Mizuno,M., Li,Z. and Ohta,Y.
  TITLE     Hyper-production and Characterization of the iota-Carrageenase
            Useful for iota-Carrageenan Oligosaccharide Production from a
            Deep-sea Bacterium, Microbulbifer thermotolerans JAMB-A94T, and
            Insight into the Unusual Catalytic Mechanism
  JOURNAL   Mar. Biotechnol. 13, 411-422 (2011)
  REMARK    DOI:10.1007/s10126-010-9312-0
COMMENT     
FEATURES             Location/Qualifiers
     source          1..1710
                     /db_xref="taxon:252514"
                     /mol_type="genomic DNA"
                     /note="type strain of Microbulbifer thermotolerans"
                     /organism="Microbulbifer thermotolerans"
                     /strain="JAMB-A94"
     CDS             1..1710
                     /codon_start=1
                     /gene="cgiA"
                     /product="iota-carrageenase"
                     /protein_id="BAJ40863.1"
                     /transl_table=11
                     /translation="MKIKEALLATSVLAIFCTPLFAGQDEVASQYDLSGTGYGQDINT
                     FAHKYIQGASPYFENRVFDQQPEELYPARNYTSQTNDTQRLLAALRRASQEDSDGGVV
                     ILRAAKNGGNNRYRLDRVDMPSNVRLEVDPDVTIEMIGNLKTSGSNQFLFSIGRSPNV
                     NNLIRERIRNVEITSTHPTRRFTIDAKTNMPISYGVHGDKTKDTAINYTRAIPIGIYY
                     AENFAISGIRIIDNHTEHVGISLYPDCDYKDGALADRNGTNGNPQDDVWLGRDGQPLP
                     MDSEGNYVDDKGRIIPDEEAIIRNLTYGRTPVKGTIENIVLQNSHTGYGAVQIFGGDW
                     IYINNIKAVKGVGVRIEAGNGSATDNINRAGPYLSSINNVEVHNVTVTKGFTGVWLKA
                     HGKINRNITVSNVTAIDSGTAVITDKSRMNPHSRDFINGRFENLTIKGNIKLIRTNKN
                     AGKAVAEVGNMLTYFMSPAERDNLTDRNGDGRITQADLPHNRSGRRWYLMQPTSPMII
                     ASQRNANEIGDTSSDIKAEEAGYYPVDFSSAVVTSENPLRPELILFNEDSRNLNGGNS
                     NRL"
BASE COUNT          512 a          433 c          395 g          370 t
ORIGIN      
        1 atgaaaataa aagaagcact actcgccacg tcagttttgg cgattttttg tacacctcta
       61 ttcgccggtc aagatgaggt tgcgagccaa tacgacctaa gcgggacggg atacggtcaa
      121 gatatcaaca cctttgcaca caagtatata caaggggctt ctccatactt cgaaaaccgt
      181 gtttttgacc agcagccaga agagctgtac ccggctagaa actacacatc acagactaat
      241 gacacccagc gcttactggc cgccctacgg cgagcgagcc aagaagacag tgatggaggg
      301 gtagttatac tccgtgcagc aaaaaacggc ggcaacaacc gttaccggtt agaccgcgtg
      361 gacatgccct ccaatgtacg cttagaggta gacccggatg tcactataga aatgattggc
      421 aatctgaaga cctctggatc aaaccagttc cttttcagta tcggccgcag ccctaacgtc
      481 aacaatctaa tcagagagcg tattcgcaac gtggaaatta cctccacgca ccctacccgc
      541 cgattcacca tcgatgcaaa aaccaatatg cctatctcct acggtgttca cggagataaa
      601 accaaggata cagccatcaa ctacacccgt gctataccaa tcggtatcta ttacgcggaa
      661 aactttgcta tttcaggaat acgaataatc gacaatcaca ccgagcatgt cggcatttcc
      721 ctttacccag actgtgacta taaagatggc gctctggctg atcgcaatgg aaccaatgga
      781 aatccacaag acgatgtttg gcttggtcgg gatggtcaac cactccccat ggactccgaa
      841 ggaaactatg tcgatgataa gggccgaata atacccgacg aggaggctat aatccgcaat
      901 ctcacttacg gacgcacccc cgtaaaagga actattgaaa atattgtcct gcaaaattcg
      961 cacacaggtt atggggccgt tcagatattt ggtggtgatt ggatatatat caataatatc
     1021 aaagcggtaa aaggcgtcgg cgtcagaatt gaagcaggaa acggctcggc gacagacaat
     1081 atcaaccggg caggccccta tttatcgtct atcaacaatg tagaagtaca caacgtgact
     1141 gtcaccaagg ggttcaccgg tgtttggctg aaggcgcacg ggaaaattaa cagaaatatt
     1201 accgtcagca atgtcactgc gatcgatagt ggcacagcgg ttatcaccga caaaagtcgc
     1261 atgaatcccc acagccgaga ctttatcaat ggccgcttcg aaaaccttac catcaaagga
     1321 aacataaaac tgatcaggac aaataaaaac gcaggcaagg ccgtagcgga agttggcaat
     1381 atgctcacat atttcatgtc accggcggag cgtgacaatt tgactgaccg gaacggcgac
     1441 gggcgcatca cgcaggcgga tcttccgcac aatcgctctg gacgtcgctg gtatctgatg
     1501 caacccactt ctccgatgat tatcgcctca cagagaaatg ccaatgaaat tggtgatacc
     1561 tcgtcagata ttaaagccga ggaagcaggt tattatcctg tggacttctc atctgccgtt
     1621 gtgacgagcg aaaatccgct gcgtcccgag ttaatattat tcaacgagga ttcgcgcaac
     1681 ttaaatggag gcaattccaa ccggctctga
//