LOCUS       AA990050                 232 bp    mRNA    linear   EST 02-JUN-1998
DEFINITION  ua58b03.r1 Soares_thymus_2NbMT Mus musculus cDNA clone
            IMAGE:1361645 5' similar to gb:M97287 DNA-BINDING PROTEIN SATB1
            (HUMAN); gb:U05252 Mus musculus nuclear matrix attachment
            DNA-binding protein SATB1 (MOUSE), mRNA sequence.
ACCESSION   AA990050
VERSION     AA990050.1
DBLINK      BioSample: SAMN00155149
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T.,
            Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M.,
            Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B.,
            Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and
            Waterston,R.
  TITLE     The WashU-HHMI Mouse EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Marra M/Mouse EST Project
            WashU-HHMI Mouse EST Project
            Washington University School of MedicineP
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: mouseest@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            MGI:699685
            Trace considered overall poor quality
            Seq primer: -28m13 rev2 ET from Amersham
            Sequence considered overall poor quality.
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="taxon:10090"
                     /clone="IMAGE:1361645"
                     /sex="male"
                     /tissue_type="Thymus"
                     /clone_lib="SAMN00155149 Soares_thymus_2NbMT"
                     /dev_stage="4 weeks"
                     /lab_host="DH10B"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     1st strand cDNA was primed with a Not I - oligo(dT) primer
                     [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCGTTTTTTTTTTTTTTTTTTTTTTTTT
                     3']; double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Not I and cloned into the Not I
                     and Eco RI sites of the modified pT7T3 vector. RNA
                     provided by Dr. Bertrand Jordan. Library went through two
                     rounds of normalization, and was constructed by Bento
                     Soares and M.Fatima Bonaldo."
BASE COUNT           46 a           51 c           77 g           58 t
ORIGIN      
        1 catttcgctg ccgagagatt tgcagagtac gtgctgttca caatggagga gatcatgctc
       61 tttgaaaggg ggcactcctt ggccagtgaa gtcgggttca gatcgtgcgg tcagtgcgtc
      121 accggaggcc ttactgtgtg tgtgggccgg tgtcaagaga gaaggctgct ggtttccggc
      181 gactgtggaa ctgcattttc atggtgccac atgaacacag cctgaagcgt gt
//